Bacterin was prepared by formalin-inactivating the virulent strain of Vibrio hollisae isolated from diseased Seriola dumerili （amberjack） suffering from vibriosis. Healthy S. dumeriIi were vaccinated by respective p...Bacterin was prepared by formalin-inactivating the virulent strain of Vibrio hollisae isolated from diseased Seriola dumerili （amberjack） suffering from vibriosis. Healthy S. dumeriIi were vaccinated by respective procedures of intramuscular injection, immersion, and orally administration. Results of the three different vaccinations were compared. Blood was drawn from the vaccinated fish every 7 days, and the antibody titers and lysozyme activities of the sera were determined. The antibody titer of injected fish was 1：40 at 7 d, and reached its peak of 1：320 at 28 d, while the fish vaccinated by immersion and orally administration exhibited weak antibody responses, the antibody titres of 〈1：10, 1：20, 1：160 were observed at 7 d, 14 d, 35 d respectively. Compared with the control, the vaccinated fish exhibited significantly higher lysozyme activities （P〈0.05）. Upon challenge with virulent strain, the relative percent survival （RPS） of injected, immersed and oral administrated fish were 75%, 45%, and 40% respectively, and the injected fish showed significantly higher RPS than immersed and oral administrated fish. The results suggested that vaccination of S. dumerili by the injection would be the best strategy to prevent the vibriosis in S. dumerili farm.展开更多
Sex-biased microRNAs(miRNAs)influence gonadal development in fish by directly targeting genes associated with estrogen production pathways.WW domain-binding protein 2(WBP2)functions as a crucial transcriptional co-act...Sex-biased microRNAs(miRNAs)influence gonadal development in fish by directly targeting genes associated with estrogen production pathways.WW domain-binding protein 2(WBP2)functions as a crucial transcriptional co-activator of the estrogen and progesterone receptors(PGR).This study investigates the direct modulation of a sex-biased miR-133b on wbp2 and its regulatory role in gonadal development in fish,the greater amberjack(Seriola dumerili).Using dual-luciferase reporter assays,we demonstrate that wbp2 is a direct target of miR-133b,with miR-133b-3p binding to the 3'untranslated region(3'UTR)of wbp2.In vitro,miR-133b mimic significantly downregulate wbp2 expression,while the miR-133b inhibitor increase wbp2 levels.Consistently,in vivo,wbp2 expression is upregulated following antagomir-133b treatment and downregulated following agomir-133b.RNA fluorescence in situ hybridization(RNA-FISH)results reveal miR-133b and wbp2 co-localization in ovarian interstitial cells.Notably,phylogenetic analysis indicates that miR-133b-3p and wbp2 are highly conserved among bony fish species.Additionally,dual-luciferase assays in other bony fish species including Oreochromis niloticus and Danio rerio also confirm the targeting effect of miR-133b-3p on wbp2,suggesting that this regulatory mechanism is conserved across bony fish.This research provides a theoretical foundation for further exploration of non-coding RNA-mediated regulation in gonadal development in teleost.展开更多
基金Supported by NSFC (No. B9910030)Key Technologies Program of Fujian Province (No. 2000z080)
文摘Bacterin was prepared by formalin-inactivating the virulent strain of Vibrio hollisae isolated from diseased Seriola dumerili （amberjack） suffering from vibriosis. Healthy S. dumeriIi were vaccinated by respective procedures of intramuscular injection, immersion, and orally administration. Results of the three different vaccinations were compared. Blood was drawn from the vaccinated fish every 7 days, and the antibody titers and lysozyme activities of the sera were determined. The antibody titer of injected fish was 1：40 at 7 d, and reached its peak of 1：320 at 28 d, while the fish vaccinated by immersion and orally administration exhibited weak antibody responses, the antibody titres of 〈1：10, 1：20, 1：160 were observed at 7 d, 14 d, 35 d respectively. Compared with the control, the vaccinated fish exhibited significantly higher lysozyme activities （P〈0.05）. Upon challenge with virulent strain, the relative percent survival （RPS） of injected, immersed and oral administrated fish were 75%, 45%, and 40% respectively, and the injected fish showed significantly higher RPS than immersed and oral administrated fish. The results suggested that vaccination of S. dumerili by the injection would be the best strategy to prevent the vibriosis in S. dumerili farm.
基金The Fund of Southern Marine Science and Engineering Guangdong Laboratory(Zhanjiang)under contract No.ZJW-2023-01the Guangdong Basic and Applied Basic Research Foundation under contract Nos 2023A1515010576 and 2024A1515012859the Marine Youth Talent Project of Zhanjiang under contract No.2023E0006.
文摘Sex-biased microRNAs(miRNAs)influence gonadal development in fish by directly targeting genes associated with estrogen production pathways.WW domain-binding protein 2(WBP2)functions as a crucial transcriptional co-activator of the estrogen and progesterone receptors(PGR).This study investigates the direct modulation of a sex-biased miR-133b on wbp2 and its regulatory role in gonadal development in fish,the greater amberjack(Seriola dumerili).Using dual-luciferase reporter assays,we demonstrate that wbp2 is a direct target of miR-133b,with miR-133b-3p binding to the 3'untranslated region(3'UTR)of wbp2.In vitro,miR-133b mimic significantly downregulate wbp2 expression,while the miR-133b inhibitor increase wbp2 levels.Consistently,in vivo,wbp2 expression is upregulated following antagomir-133b treatment and downregulated following agomir-133b.RNA fluorescence in situ hybridization(RNA-FISH)results reveal miR-133b and wbp2 co-localization in ovarian interstitial cells.Notably,phylogenetic analysis indicates that miR-133b-3p and wbp2 are highly conserved among bony fish species.Additionally,dual-luciferase assays in other bony fish species including Oreochromis niloticus and Danio rerio also confirm the targeting effect of miR-133b-3p on wbp2,suggesting that this regulatory mechanism is conserved across bony fish.This research provides a theoretical foundation for further exploration of non-coding RNA-mediated regulation in gonadal development in teleost.
