Objective It is well known that a dual trigger treatment can improve clinical outcomes of in vitro fertilization(IVF)in high or normal ovarian responders.However,it is not clear whether dual triggering also benefits p...Objective It is well known that a dual trigger treatment can improve clinical outcomes of in vitro fertilization(IVF)in high or normal ovarian responders.However,it is not clear whether dual triggering also benefits patients with diminished ovarian reserve(DOR).The aim of this study was to investigate whether a dual trigger treatment of gonadotropin-releasing hormone(GnRH)agonist combined with human chorionic gonadotropin(hCG)for final follicular maturation improves the cumulative live birth rate(CLBR)during the GnRH-antagonist cycle in patients with DOR.Methods This retrospective study included patients with DOR who received a GnRH-antagonist protocol during IVF and intracytoplasmic sperm injection(IVF-ICSI)cycles at Peking University People’s Hospital from January 1,2017 through December 31,2017.Oocyte maturation was triggered by GnRH combined with hCG(n=110)or hCG alone(n=71).Embryos were transferred on the third day after oocyte retrieval or during a subsequent freeze-thaw cycle.Patients were followed up for 3 years.Results The dual trigger treatment did not affect CLBR,which is an overall determinant of the success rate of assisted reproductive technology(ART).Women in the dual trigger group had significantly higher rates of fertilization than those in the hCG group(90.1%vs.83.9%,P=0.040).Conclusion Dual trigger with GnRH agonist and hCG did not improve CLBR in patients with DOR,but did slightly improve fertilization rate,oocyte count,and embryo quality.展开更多
BACKGROUND Borderline form of empty follicle syndrome is a condition in which only a few mature or immature oocytes are recovered after meticulous follicular aspiration,despite adequate ovarian response to stimulation...BACKGROUND Borderline form of empty follicle syndrome is a condition in which only a few mature or immature oocytes are recovered after meticulous follicular aspiration,despite adequate ovarian response to stimulation.It is a rare phenomenon with an unclear cause.Currently,the condition still lacks effective treatment.CASE SUMMARY A patient with secondary infertility who had undergone three cycles of assisted reproductive technique(ART)is described.With regard to good follicular response,two oocytes were obtained in the first two ART cycles,but no embryo was formed.In the third ART cycle,which is the subject of this study,ovulation was induced by dual trigger of a supernormal dose of human chorionic gonadotropin(HCG)combined with a delayed oocyte retrieval approach.The method involved administration of gonadotropin-releasing hormone agonist,recombinant HCG,and urinary HCG 39 h before ovum pick-up.Ten oocytes were recovered,two out of three mature eggs were fertilized after intracytoplasmic sperm injection,resulting in two embryos that were subsequently cryopreserved.The case report guidelines have been used herein to present the first case of this novel dual trigger method.CONCLUSION This approach provides a new treatment option for patients with a similar condition in the future.This study can also inspire further investigation on the effects of variousβ-HCG serum levels 36 h after intramuscular HCG administration.展开更多
<strong><em>Objective</em></strong><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">:<...<strong><em>Objective</em></strong><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">:</span></span></span><span><span><span style="font-family:""><span style="font-family:Verdana;"> To evaluate the benefit of dual trigger (hCG + GnRH agonist) in patients underwent controlled ovarian stimulation for IVF in an antagonist protocol. </span><b><i><span style="font-family:Verdana;">Methods</span></i></b><span style="font-family:Verdana;">:</span><span style="font-family:Verdana;"> A retrospective case control study was performed (January 2017 to March 2019) in a single IVF center. The dual trigger group (n = 17), ovulation trigger was achieved with both hCG and GnRH agonist while in the single trigger group (n = 34), it was achieved by hCG alone. The first endpoint was the number of mature oocytes retrieved;the secondary endpoints were total number of oocytes retrieved, the number of cleaved embryos obtained (day 3) and blastocysts (day 5/day 6), the number of embryos transferred, the ongoing-pregnancy/miscarriage rate. </span><b><i><span style="font-family:Verdana;">Results</span></i></b><span style="font-family:Verdana;">:</span><span style="font-family:Verdana;"> The dual vs. the single group showed the followings. The number of retrieved oocytes of 7.1 vs. 6.4 (p = 0.68);mature oocytes of 4.6 vs. 4.1 (p = 0.62), day-3-embryos of 2.9 vs. 2.0 (p = 0.2), day-5/6-embryos of 0.3 vs. 0.03 (p = 0.13), transferred embryos of 2.1 vs. 1.8 (p = 0.48);ongoing pregnancy of 1 vs. 9 (p = 0.14);miscarriage of 0 vs. 2 (p = 1). </span><b><i><span style="font-family:Verdana;">Conclusion</span></i></b><span style="font-family:Verdana;">:</span><span style="font-family:Verdana;"> A dual trigger showed no additional clinical benefits. Future large studies are needed to demonstrate a real clinical advantage.</span></span></span></span>展开更多
目的探讨促性腺激素激动剂(GnRH-a)联合小剂量人绒毛膜促性腺激素(hCG)诱发排卵对卵巢高反应患者体外受精-胚胎移植的临床结局影响。方法回顾性分析2016年12月至2017年12月在南京医科大学附属妇产医院本中心采取GnRH拮抗剂方案进行控制...目的探讨促性腺激素激动剂(GnRH-a)联合小剂量人绒毛膜促性腺激素(hCG)诱发排卵对卵巢高反应患者体外受精-胚胎移植的临床结局影响。方法回顾性分析2016年12月至2017年12月在南京医科大学附属妇产医院本中心采取GnRH拮抗剂方案进行控制性超促排卵,行体外受精/卵胞浆内单精子注射(IVF/ICSI)助孕的546例患者的临床资料,将因卵巢高反应分别使用GnRH-a联合5000 U hCG促排卵(hCG大剂量组,n=159)及GnRH-a联合2000 U hCG促排卵(hCG小剂量组,n=212)的患者作为研究组,同期采用标准的hCG 10000 U促排卵的患者作为对照组(n=175),比较各组患者的治疗结局。结果三组患者促性腺激素(Gn)启动剂量及用药时间比较,差异均无统计学意义(P>0.05)。hCG大剂量组患者hCG促排卵日血清雌二醇(E2)水平[(17513.52±4846.85)pmol/L]及hCG小剂量组患者[(20384.11±7754.78)pmol/L]显著高于对照组患者[(16678.67±3826.79)pmol/L](P=0.000)。两研究组的获卵数、2PN数、胚胎数、优胚数及优胚率方案高于对照组(P<0.05),同时hCG大剂量组优胚率均高于hCG小剂量组(P<0.05)。hCG大剂量组、小剂量组与对照组三组间卵巢过度刺激综合征(OHSS)率(1.3%、0.5%、2.9%)发生率比较,差异无统计学意义(P>0.05)。结论对于GnRH拮抗剂方案的高反应患者,采用减量联合促排卵方案,可提高优胚率及可移植胚胎数,不增加OHSS发生率。展开更多
基金supported by grants from the Scientific Research Development Fund of Peking University People’s Hospital(No.RDY2020-27)Clinical Medicine Plus X-Young Scholars Project,Peking University,and the Fundamental Research Funds for the Central Universities(No.PKU2021LCXQ020).
文摘Objective It is well known that a dual trigger treatment can improve clinical outcomes of in vitro fertilization(IVF)in high or normal ovarian responders.However,it is not clear whether dual triggering also benefits patients with diminished ovarian reserve(DOR).The aim of this study was to investigate whether a dual trigger treatment of gonadotropin-releasing hormone(GnRH)agonist combined with human chorionic gonadotropin(hCG)for final follicular maturation improves the cumulative live birth rate(CLBR)during the GnRH-antagonist cycle in patients with DOR.Methods This retrospective study included patients with DOR who received a GnRH-antagonist protocol during IVF and intracytoplasmic sperm injection(IVF-ICSI)cycles at Peking University People’s Hospital from January 1,2017 through December 31,2017.Oocyte maturation was triggered by GnRH combined with hCG(n=110)or hCG alone(n=71).Embryos were transferred on the third day after oocyte retrieval or during a subsequent freeze-thaw cycle.Patients were followed up for 3 years.Results The dual trigger treatment did not affect CLBR,which is an overall determinant of the success rate of assisted reproductive technology(ART).Women in the dual trigger group had significantly higher rates of fertilization than those in the hCG group(90.1%vs.83.9%,P=0.040).Conclusion Dual trigger with GnRH agonist and hCG did not improve CLBR in patients with DOR,but did slightly improve fertilization rate,oocyte count,and embryo quality.
文摘BACKGROUND Borderline form of empty follicle syndrome is a condition in which only a few mature or immature oocytes are recovered after meticulous follicular aspiration,despite adequate ovarian response to stimulation.It is a rare phenomenon with an unclear cause.Currently,the condition still lacks effective treatment.CASE SUMMARY A patient with secondary infertility who had undergone three cycles of assisted reproductive technique(ART)is described.With regard to good follicular response,two oocytes were obtained in the first two ART cycles,but no embryo was formed.In the third ART cycle,which is the subject of this study,ovulation was induced by dual trigger of a supernormal dose of human chorionic gonadotropin(HCG)combined with a delayed oocyte retrieval approach.The method involved administration of gonadotropin-releasing hormone agonist,recombinant HCG,and urinary HCG 39 h before ovum pick-up.Ten oocytes were recovered,two out of three mature eggs were fertilized after intracytoplasmic sperm injection,resulting in two embryos that were subsequently cryopreserved.The case report guidelines have been used herein to present the first case of this novel dual trigger method.CONCLUSION This approach provides a new treatment option for patients with a similar condition in the future.This study can also inspire further investigation on the effects of variousβ-HCG serum levels 36 h after intramuscular HCG administration.
文摘<strong><em>Objective</em></strong><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">:</span></span></span><span><span><span style="font-family:""><span style="font-family:Verdana;"> To evaluate the benefit of dual trigger (hCG + GnRH agonist) in patients underwent controlled ovarian stimulation for IVF in an antagonist protocol. </span><b><i><span style="font-family:Verdana;">Methods</span></i></b><span style="font-family:Verdana;">:</span><span style="font-family:Verdana;"> A retrospective case control study was performed (January 2017 to March 2019) in a single IVF center. The dual trigger group (n = 17), ovulation trigger was achieved with both hCG and GnRH agonist while in the single trigger group (n = 34), it was achieved by hCG alone. The first endpoint was the number of mature oocytes retrieved;the secondary endpoints were total number of oocytes retrieved, the number of cleaved embryos obtained (day 3) and blastocysts (day 5/day 6), the number of embryos transferred, the ongoing-pregnancy/miscarriage rate. </span><b><i><span style="font-family:Verdana;">Results</span></i></b><span style="font-family:Verdana;">:</span><span style="font-family:Verdana;"> The dual vs. the single group showed the followings. The number of retrieved oocytes of 7.1 vs. 6.4 (p = 0.68);mature oocytes of 4.6 vs. 4.1 (p = 0.62), day-3-embryos of 2.9 vs. 2.0 (p = 0.2), day-5/6-embryos of 0.3 vs. 0.03 (p = 0.13), transferred embryos of 2.1 vs. 1.8 (p = 0.48);ongoing pregnancy of 1 vs. 9 (p = 0.14);miscarriage of 0 vs. 2 (p = 1). </span><b><i><span style="font-family:Verdana;">Conclusion</span></i></b><span style="font-family:Verdana;">:</span><span style="font-family:Verdana;"> A dual trigger showed no additional clinical benefits. Future large studies are needed to demonstrate a real clinical advantage.</span></span></span></span>
文摘目的探讨促性腺激素激动剂(GnRH-a)联合小剂量人绒毛膜促性腺激素(hCG)诱发排卵对卵巢高反应患者体外受精-胚胎移植的临床结局影响。方法回顾性分析2016年12月至2017年12月在南京医科大学附属妇产医院本中心采取GnRH拮抗剂方案进行控制性超促排卵,行体外受精/卵胞浆内单精子注射(IVF/ICSI)助孕的546例患者的临床资料,将因卵巢高反应分别使用GnRH-a联合5000 U hCG促排卵(hCG大剂量组,n=159)及GnRH-a联合2000 U hCG促排卵(hCG小剂量组,n=212)的患者作为研究组,同期采用标准的hCG 10000 U促排卵的患者作为对照组(n=175),比较各组患者的治疗结局。结果三组患者促性腺激素(Gn)启动剂量及用药时间比较,差异均无统计学意义(P>0.05)。hCG大剂量组患者hCG促排卵日血清雌二醇(E2)水平[(17513.52±4846.85)pmol/L]及hCG小剂量组患者[(20384.11±7754.78)pmol/L]显著高于对照组患者[(16678.67±3826.79)pmol/L](P=0.000)。两研究组的获卵数、2PN数、胚胎数、优胚数及优胚率方案高于对照组(P<0.05),同时hCG大剂量组优胚率均高于hCG小剂量组(P<0.05)。hCG大剂量组、小剂量组与对照组三组间卵巢过度刺激综合征(OHSS)率(1.3%、0.5%、2.9%)发生率比较,差异无统计学意义(P>0.05)。结论对于GnRH拮抗剂方案的高反应患者,采用减量联合促排卵方案,可提高优胚率及可移植胚胎数,不增加OHSS发生率。
