随着全球森林和农作物病虫害问题的日益严重,传统化学农药防治方法因其环境污染和生物抗性问题而受到公众的质疑。近年来,越来越多的研究者将目光转向RNA干扰(RNAi)技术,利用RNAi的特性控制农林植物病虫害,并减少对环境的负面影响。本...随着全球森林和农作物病虫害问题的日益严重,传统化学农药防治方法因其环境污染和生物抗性问题而受到公众的质疑。近年来,越来越多的研究者将目光转向RNA干扰(RNAi)技术,利用RNAi的特性控制农林植物病虫害,并减少对环境的负面影响。本综述聚焦于RNAi技术的原理、外源性dsRNA的应用,并分析目前面临的机遇和挑战。同时探讨纳米颗粒在dsRNA递送应用方面的研究进展,如壳聚糖纳米颗粒(CNPs)、层状氢氧化物(LDH)和星形阳离子聚合物(SPc)等,这些纳米颗粒可以有效保护dsRNA并提高其传递效率。这些研究为农林业可持续发展提供了新的解决方案,有助于保护森林植被和提高农作物的产量与质量。With the increasing problems of pest and disease of global forests and crops, traditional chemical pesticide control methods are being questioned due to environmental pollution and biological resistance issues. However, recently, more researchers have turned to RNA interference (RNAi) technology, using the properties of RNAi to control plant pests and diseases, reducing the negative impact on the environments. This review focuses on the principles of RNAi technology, the applications of exogenous double-stranded RNA (dsRNA), while analyzing the associated opportunities and challenges. Additionally, we explored the research progress in nanoparticle delivery systems for dsRNA, such as chitosan nanoparticles (CNPs), layered double hydroxide (LDH), and star polycation (SPc), which effectively protect dsRNA and enhance its delivery efficiency. These studies provide innovative solutions for sustainable agricultural and forestry development, contributing to the preservation of forest and the improvement of crop yields and quality.展开更多
Insect pests cause severe crop damage,resulting in substantial economic losses and threats to global food security.Conventional insecticides are low-cost chemical agents that kill the target insects and some non-speci...Insect pests cause severe crop damage,resulting in substantial economic losses and threats to global food security.Conventional insecticides are low-cost chemical agents that kill the target insects and some non-specific beneficial organisms.Due to their toxic and non-biodegradable nature,these conventional insecticides persist in the environment,thus causing pollution and accumulating in the food chain.The development of novel insecticidal products based on double-stranded(dsRNA)-based RNA interference(RNAi)technology is a sustainable tool to effectively control insect pests.The dsRNA-based insecticides are known for their specificity,non-toxicity,and biodegradability.The current review introduces the dsRNA-based RNAi technique as a novel tool to control crop insect pests.The review highlights the mechanism behind dsRNA uptake into insect cells.Furthermore,it discusses the commercial aspects of different dsRNA-based products available in the market,their penetration rates,and public acceptance.The review details the latest developments in the field and the regulatory landscape regarding the technology.The advantages and limitations of dsRNA-based insecticides are discussed,and future research directions to overcome the potential challenges have been briefly suggested.The dsRNA-based insecticidal products may be a better alternative to conventional insecticides,thus delineating the resistance among insects and increasing agricultural productivity.展开更多
dsRNA segment Ⅴ was separated from Bombyx mori cytoplasmic polyhedrosis virus(BmCPV)of Chinese Guangzhou strainAfter ligation with a ssDNA adaptor,the cDNA fragments overlapping full-length segment Ⅴ of BmCPV ...dsRNA segment Ⅴ was separated from Bombyx mori cytoplasmic polyhedrosis virus(BmCPV)of Chinese Guangzhou strainAfter ligation with a ssDNA adaptor,the cDNA fragments overlapping full-length segment Ⅴ of BmCPV were acquired by RT-PCR amplificationSegment Ⅴ is 2852 nucleotides long and possesses a single open reading frame encoding a putative protein of 881 amino acidsComparison of amino acid sequences shows 97% homology with BmCPV Japanese isolate segments Ⅴ,86% and 36% with LdCPV-1 and LdCPV-14 segments Ⅴ respectivelyPart of sequence of BmCPV segment Ⅴ exhibits high homology with 2Apro motif of picornavirus members,which may suggest that there might exist some affinities evolutionally between both of展开更多
文摘随着全球森林和农作物病虫害问题的日益严重,传统化学农药防治方法因其环境污染和生物抗性问题而受到公众的质疑。近年来,越来越多的研究者将目光转向RNA干扰(RNAi)技术,利用RNAi的特性控制农林植物病虫害,并减少对环境的负面影响。本综述聚焦于RNAi技术的原理、外源性dsRNA的应用,并分析目前面临的机遇和挑战。同时探讨纳米颗粒在dsRNA递送应用方面的研究进展,如壳聚糖纳米颗粒(CNPs)、层状氢氧化物(LDH)和星形阳离子聚合物(SPc)等,这些纳米颗粒可以有效保护dsRNA并提高其传递效率。这些研究为农林业可持续发展提供了新的解决方案,有助于保护森林植被和提高农作物的产量与质量。With the increasing problems of pest and disease of global forests and crops, traditional chemical pesticide control methods are being questioned due to environmental pollution and biological resistance issues. However, recently, more researchers have turned to RNA interference (RNAi) technology, using the properties of RNAi to control plant pests and diseases, reducing the negative impact on the environments. This review focuses on the principles of RNAi technology, the applications of exogenous double-stranded RNA (dsRNA), while analyzing the associated opportunities and challenges. Additionally, we explored the research progress in nanoparticle delivery systems for dsRNA, such as chitosan nanoparticles (CNPs), layered double hydroxide (LDH), and star polycation (SPc), which effectively protect dsRNA and enhance its delivery efficiency. These studies provide innovative solutions for sustainable agricultural and forestry development, contributing to the preservation of forest and the improvement of crop yields and quality.
文摘Insect pests cause severe crop damage,resulting in substantial economic losses and threats to global food security.Conventional insecticides are low-cost chemical agents that kill the target insects and some non-specific beneficial organisms.Due to their toxic and non-biodegradable nature,these conventional insecticides persist in the environment,thus causing pollution and accumulating in the food chain.The development of novel insecticidal products based on double-stranded(dsRNA)-based RNA interference(RNAi)technology is a sustainable tool to effectively control insect pests.The dsRNA-based insecticides are known for their specificity,non-toxicity,and biodegradability.The current review introduces the dsRNA-based RNAi technique as a novel tool to control crop insect pests.The review highlights the mechanism behind dsRNA uptake into insect cells.Furthermore,it discusses the commercial aspects of different dsRNA-based products available in the market,their penetration rates,and public acceptance.The review details the latest developments in the field and the regulatory landscape regarding the technology.The advantages and limitations of dsRNA-based insecticides are discussed,and future research directions to overcome the potential challenges have been briefly suggested.The dsRNA-based insecticidal products may be a better alternative to conventional insecticides,thus delineating the resistance among insects and increasing agricultural productivity.
文摘dsRNA segment Ⅴ was separated from Bombyx mori cytoplasmic polyhedrosis virus(BmCPV)of Chinese Guangzhou strainAfter ligation with a ssDNA adaptor,the cDNA fragments overlapping full-length segment Ⅴ of BmCPV were acquired by RT-PCR amplificationSegment Ⅴ is 2852 nucleotides long and possesses a single open reading frame encoding a putative protein of 881 amino acidsComparison of amino acid sequences shows 97% homology with BmCPV Japanese isolate segments Ⅴ,86% and 36% with LdCPV-1 and LdCPV-14 segments Ⅴ respectivelyPart of sequence of BmCPV segment Ⅴ exhibits high homology with 2Apro motif of picornavirus members,which may suggest that there might exist some affinities evolutionally between both of
