The interaction of Ru(phen) 2(dppz) 2+ (phen=1,10 phenanthroline,dppz=dipyrido[3,2 a:2′,3′ c]phenazine) and the calfthymus DNA were studied with fluorescence and ultraviolet visible absorption spectroscopy....The interaction of Ru(phen) 2(dppz) 2+ (phen=1,10 phenanthroline,dppz=dipyrido[3,2 a:2′,3′ c]phenazine) and the calfthymus DNA were studied with fluorescence and ultraviolet visible absorption spectroscopy. The ultraviolet visible absorption spectrum of Ru(phen) 2(dppz) 2+ , calfthymus DNA, and their interaction demonstrated that Ru(phen) 2(dppz) 2+ intercalated into the double helix of DNA via the ligand dppz. Fe(CN) 6 4- and NaCl can not quench the fluorescence of Ru(phen) 2(dppz) 2+ DNA, which was in agreement with the intercalation mode.展开更多
Because of the high affinity(KB≥10^(6)L≥mol^(-1))between Ru(phen)_(2)(dppz)^(2+)and DNA,the adding of DNA in the solution of Ru(phen)_(2)(dppz)^(2+)-SDS makes the dissociation of Ru(phen)_(2)(dppz)^(2+)-SDS,and resu...Because of the high affinity(KB≥10^(6)L≥mol^(-1))between Ru(phen)_(2)(dppz)^(2+)and DNA,the adding of DNA in the solution of Ru(phen)_(2)(dppz)^(2+)-SDS makes the dissociation of Ru(phen)_(2)(dppz)^(2+)-SDS,and results in decrease of the resonance light scattering(RLS)signals and increase of the absorbance.Based on this,a novel method is proposed for DNA assay.展开更多
文摘The interaction of Ru(phen) 2(dppz) 2+ (phen=1,10 phenanthroline,dppz=dipyrido[3,2 a:2′,3′ c]phenazine) and the calfthymus DNA were studied with fluorescence and ultraviolet visible absorption spectroscopy. The ultraviolet visible absorption spectrum of Ru(phen) 2(dppz) 2+ , calfthymus DNA, and their interaction demonstrated that Ru(phen) 2(dppz) 2+ intercalated into the double helix of DNA via the ligand dppz. Fe(CN) 6 4- and NaCl can not quench the fluorescence of Ru(phen) 2(dppz) 2+ DNA, which was in agreement with the intercalation mode.
基金support from the National Natural Science Foundation of China(20275028)the National Key Basic Research and Development Program(973 Program 2002CB2118).
文摘Because of the high affinity(KB≥10^(6)L≥mol^(-1))between Ru(phen)_(2)(dppz)^(2+)and DNA,the adding of DNA in the solution of Ru(phen)_(2)(dppz)^(2+)-SDS makes the dissociation of Ru(phen)_(2)(dppz)^(2+)-SDS,and results in decrease of the resonance light scattering(RLS)signals and increase of the absorbance.Based on this,a novel method is proposed for DNA assay.
