Measurement precision of laser displacement sensor is subject to various factors,among which laser jitter and target tilt will directly lead to the position movement and shape variation of the laser spot,resulting in ...Measurement precision of laser displacement sensor is subject to various factors,among which laser jitter and target tilt will directly lead to the position movement and shape variation of the laser spot,resulting in displacement measurement errors,so that researchers have to do a lot of research on the spot centering algorithm to weaken the above effects,which can treat the symptoms but not the root cause.Starting from the source of the problem,this paper proposes a double focus double peak solution,which uses a reflector to change the direction of the optical path,so that the imaging spots of the designed two optical paths focus on the same CMOS,forming a double peak structure.When laser jitter or target tilt occurs,the center of the two laser spots is shifted,but they move in the same direction,while their relative position remains unchanged.Therefore,the displacement can be characterized by the relative position of the two laser spots,so that laser jitter and target tilt are suppressed from the source.However,the two spots imaged on CMOS form a non-Gaussian distributed double peak structure,so the conventional laser spot centering algorithms are no longer applicable.To this end,a double peak adaptive threshold waveform extraction method combined with grayscale gravity method is proposed for spot centering algorithm,which combines the suppression of laser jitter and target tilt from the source and the improvement of spot positioning precision which represents the displacement measurement precision,and is experimentally verified.展开更多
Satellite and terrestrial cellular networks can be integrated together to achieve extended broad-band coverage for,e.g.,maritime communication sce-narios,in the upcoming sixth-generation(6G)era.To counter spectrum sca...Satellite and terrestrial cellular networks can be integrated together to achieve extended broad-band coverage for,e.g.,maritime communication sce-narios,in the upcoming sixth-generation(6G)era.To counter spectrum scarcity,collaborative spectrum sharing is considered for hybrid satellite-terrestrial networks(HSTNs)in this paper.With only slowly-varying large-scale channel state information(CSI),joint power and channel allocation is implemented for terrestrial mobile terminals(MTs)which share the same frequency band with the satellite MTs oppor-tunistically.Specially,strict quality service assurance is adopted for terrestrial MTs under the constraint of leakage interference to satellite MTs.With the tar-get of maximizing both the number of served terres-trial MTs and the average sum transmission rate,a double-target spectrum sharing problem is formulated.To solve the complicated mixed integer programming(MIP)problem efficiently,user-centric channel pools are introduced.Simulations demonstrate that the proposed spectrum sharing scheme could achieve a significant performance gain for the HSTN.展开更多
In recent years, organic-inorganic hybrid nanocarriers are explored for effective drug delivery and preferable disease treatments. In this study, using 5-fluorouracil(5-FU)as electronegative model drug, a new type of ...In recent years, organic-inorganic hybrid nanocarriers are explored for effective drug delivery and preferable disease treatments. In this study, using 5-fluorouracil(5-FU)as electronegative model drug, a new type of organic-inorganic hybrid drug delivery system(LDH/HA-PEG/5-FU)was conceived and manufactured by the adsorption of PEGylated hyaluronic acid(HA-PEG)on the surface of layered double hydroxide(LDH, prepared via hydrothermal method)and the intercalation of 5-FU in the interlamination of LDH via ion exchange strategy. The drug loading amount of LDH/HA-PEG/5-FU achieved as high as 34.2%. LDH, LDH/5-FU and LDH/HA-PEG/5-FU were characterized by FT-IR, XRD, TGA, laser particle size analyzer and SEM. With the benefit of p Hdegradable feature of LDH and enzyme-degradable feature of HA, LDH/HA-PEG/5-FU showed p H-degradable and enzyme-degradable capacity in in vitro drug release. Moreover, the drug carrier LDH/HA-PEG contained biocompatible PEG and tumor-targeted HA, resulting in lower cytotoxicity and better endocytosis compared with LDH in vitro. It was suggested that the organic-inorganic hybrid drug delivery system, which was endowed with the properties of controlled release, low toxicity and tumor-targeting delivery for ameliorative cancer therapy, was advisable and might be applied further to fulfill other treatments.展开更多
We present a gain adaptive tuning method for fiber Raman amplifier(FRA) using two-stage neural networks(NNs) and double weights updates. After training the connection weights of two-stage NNs separately in training ph...We present a gain adaptive tuning method for fiber Raman amplifier(FRA) using two-stage neural networks(NNs) and double weights updates. After training the connection weights of two-stage NNs separately in training phase, the connection weights of the unified NN are updated again in verification phase according to error between the predicted and target gains to eliminate the inherent error of the NNs. The simulation results show that the mean of root mean square error(RMSE) and maximum error of gains are 0.131 d B and 0.281 d B, respectively. It shows that the method can realize adaptive adjustment function of FRA gain with high accuracy.展开更多
The CRISPR/Cas9 system has been tailored to a revolutionary genetic tool because of its remarkable simplicity and efficacy.While complex genome editing in the mouse since the 1990 s has been dominated by the use of em...The CRISPR/Cas9 system has been tailored to a revolutionary genetic tool because of its remarkable simplicity and efficacy.While complex genome editing in the mouse since the 1990 s has been dominated by the use of embryonic stem（ES） cells,CRISPR/Cas9 now offers a versatile and fast approach to precisely modify virtually any DNA regions directly in mouse zygotes.Yet,this relative simplicity does not preclude a conscientious preparatory work that is often neglected when initiating a project.Here,we describe the key steps leading to successful generation of a double knockout（KO） mouse by simultaneously targeting two homolog genes,Tmem176 a and Tmem176 b,which are located in the same genomic locus.Additionally,we show that similar efficiency can be obtained in a mixed genetic background or directly in the C57BL/6 inbred strain.Thus,presented as a detailed case study that should be helpful to the non-specialists,we focus on the genotyping strategy to anticipate the various possibilities.展开更多
Interaction between high-intensity pulsed ion beam （HIPIB） and a double-layer target with titanium film on top of aluminum substrate was simulated. The two-dimensional nonlinear thermal conduction equations, with th...Interaction between high-intensity pulsed ion beam （HIPIB） and a double-layer target with titanium film on top of aluminum substrate was simulated. The two-dimensional nonlinear thermal conduction equations, with the deposited energy in the target taken as source term, were derived and solved by finite differential method. As a result, the two-dimensional spatial and temporal evolution profiles of temperature were obtained for a titanium/aluminum double-layer target irradiated by a pulse of HIPIB. The effects of ion beam current density on the phase state of the target materials near the film and substrate interface were analyzed. Both titanium and aluminum were melted near the interface after a shot when the ion beam current density fell in the range of 100 A/cm2 to 200 A/cm2.展开更多
Gene targeting technology is an important means to investigate gene functions, but its efficiency of gene targeting is very low, especially for somatic cell targeting. Artificially induced double-strand breaks （DSB）...Gene targeting technology is an important means to investigate gene functions, but its efficiency of gene targeting is very low, especially for somatic cell targeting. Artificially induced double-strand breaks （DSB） and triplex forming oligonucleotide （TFO） are currently developed methods to improve the targeting efficiency. This paper summarized the basic principles, design ideas and application in gene targeting efficiency improvement of these two methods, analyzed and com- pared their characteristics, and finally proposed prospects for their future development.展开更多
In order to develop green good, reduce the use of chemical fertilizer and pesticide, realize ＂double reduction＂ and synergism, improve safety and quality of products, protect the ecological environment and promote t...In order to develop green good, reduce the use of chemical fertilizer and pesticide, realize ＂double reduction＂ and synergism, improve safety and quality of products, protect the ecological environment and promote the healthy and steady development of strawberry industry, the goals and key technology of fertilizer-pesticide ＂double reduction＂ and synergism for greenhouse strawberry are summed up in this paper targeting at the current situation of strawberry production and combined with the new technology achievements at home and abroad. The key technology includes six items： healthy seedling cultivating technique, soil improvement and continuous cropping obstacle treatment technique, agricultural ecological prevention tech- nique, physicochemical trap of pest control technique, biological control technique and low residual risk chemical control technique.展开更多
The purpose of initial orbit determination,especially in the case of angles-only data for observation,is to obtain an initial estimate that is close enough to the true orbit to enable subsequent precision orbit determ...The purpose of initial orbit determination,especially in the case of angles-only data for observation,is to obtain an initial estimate that is close enough to the true orbit to enable subsequent precision orbit determination processing to be successful.However,the classical angles-only initial orbit determination methods cannot deal with the observation data whose Earth-central angle is larger than 360°.In this paper,an improved double r-iteration initial orbit determination method to deal with the above case is presented to monitor geosynchronous Earth orbit objects for a spacebased surveillance system.Simulation results indicate that the improved double r-iteration method is feasible,and the accuracy of the obtained initial orbit meets the requirements of re-acquiring the object.展开更多
In the most recent decades,a major number of image encryption plans have been proposed.The vast majority of these plans reached a highsecurity level;however,their moderate speeds because of their complicated processes...In the most recent decades,a major number of image encryption plans have been proposed.The vast majority of these plans reached a highsecurity level;however,their moderate speeds because of their complicated processes made them of no use in real-time applications.Inspired by this,we propose another efficient and rapid image encryption plan dependent on the Trigonometric chaotic guide.In contrast to the most of current plans,we utilize this basic map to create just a couple of arbitrary rows and columns.Moreover,to additionally speed up,we raise the processing unit from the pixel level to the row/column level.The security of the new plot is accomplished through a substitution permutation network,where we apply a circular shift of rows and columns to break the solid connection of neighboring pixels.At that point,we join the XOR operation with modulo function to cover the pixels values and forestall any leaking of data.High-security tests and simulation analyses are carried out to exhibit that the scheme is very secure and exceptionally quick for real-time image processing at 80 fps(frames per second).展开更多
Breast cancer is a significant global concern,with limited effective treatment options.Therefore,therapies with high efficacy and low complications,unlike the existing chemotherapies,are urgently required.To address t...Breast cancer is a significant global concern,with limited effective treatment options.Therefore,therapies with high efficacy and low complications,unlike the existing chemotherapies,are urgently required.To address this issue,advances have been made in therapies targeting molecular pathways related to the murine double minute 2 protooncogene(MDM2)-tumor proteinp53(TP53)interaction.This review aims to investigate the efficacy of MDM2 inhibition in restoring TP53 activity in breast cancer cells,as evidenced by clinical studies,reviews,and trials.TP53 is a tumor suppressor and MDM2 facilitates proteasomal degradation of TP53.MDM2 and TP53 activity is tightly regulated.However,cancerous breast cells overexpress MDM2 through five hypothesized mechanisms.Consequently,TP53 levels decrease with increased tumor cell proliferation.Three strategies have been identified for controlling MDM2 upregulation in cells with wild-type or mutated TP53.MDM2 inhibitors(MDM2i)are administered in combination with existing chemotherapies to reduce their effects on healthy cells.Few clinical and preclinical studies have been conducted using MDM2i,which necessitates high-quality clinical trials to support their therapeutic potential in breast cancer therapy.展开更多
In this paper, the shortages of Wigner-Ville Distribution(WVD) double-linearity in extracting the Doppler parameters of moving-targets are discussed, especially in multi-point moving-target imaging processing based on...In this paper, the shortages of Wigner-Ville Distribution(WVD) double-linearity in extracting the Doppler parameters of moving-targets are discussed, especially in multi-point moving-target imaging processing based on the spectrum characteristics of moving-target echo signals in Synthetic Aperture Radar (SAR) imaging processing and the properties of WVD. Combined with the characteristics of Continuous Wavelet Transform (CWT), the responsibility and advantages of CWT in multi-point moving-target Doppler parameter extraction are analyzed. Finally a kind of multi-point moving-target Doppler parameter extracting algorithm based on CWT are developed, and the computer stimulating results demonstrate the correctness of the algorithm.展开更多
气候问题日趋严重,“双碳”目标引起学者的广泛关注。为分析当前国内外“双碳”领域研究前沿与热点差异,本文利用科学计量技术,针对知网和Web of Science核心数据集自2009—2022年有关“双碳”目标的文献进行时空维度内文献分布特征分...气候问题日趋严重,“双碳”目标引起学者的广泛关注。为分析当前国内外“双碳”领域研究前沿与热点差异,本文利用科学计量技术,针对知网和Web of Science核心数据集自2009—2022年有关“双碳”目标的文献进行时空维度内文献分布特征分析和研究前沿与热点动态演化分析。结果表明:“双碳”领域正处于研究浪潮阶段,自2020年后“双碳”领域发文量呈爆发式增长,研究力量集中于西方发达国家。研究侧重点存在差异,国外“双碳”领域从生物质和太阳能利用等技术角度展开研究,国内则侧重政策治理层面,低碳转型成为研究热点。本文提出“双碳”领域未来发展方向将聚焦于低碳技术和模式创新,以创新助推低碳转型。展开更多
基金the Biomedical Science and Technology Support Special Project of Shanghai Science and Technology Committee(No.20S31908300)。
文摘Measurement precision of laser displacement sensor is subject to various factors,among which laser jitter and target tilt will directly lead to the position movement and shape variation of the laser spot,resulting in displacement measurement errors,so that researchers have to do a lot of research on the spot centering algorithm to weaken the above effects,which can treat the symptoms but not the root cause.Starting from the source of the problem,this paper proposes a double focus double peak solution,which uses a reflector to change the direction of the optical path,so that the imaging spots of the designed two optical paths focus on the same CMOS,forming a double peak structure.When laser jitter or target tilt occurs,the center of the two laser spots is shifted,but they move in the same direction,while their relative position remains unchanged.Therefore,the displacement can be characterized by the relative position of the two laser spots,so that laser jitter and target tilt are suppressed from the source.However,the two spots imaged on CMOS form a non-Gaussian distributed double peak structure,so the conventional laser spot centering algorithms are no longer applicable.To this end,a double peak adaptive threshold waveform extraction method combined with grayscale gravity method is proposed for spot centering algorithm,which combines the suppression of laser jitter and target tilt from the source and the improvement of spot positioning precision which represents the displacement measurement precision,and is experimentally verified.
基金supported in part by the National Natural Science Foundation of China under Grant 62425110 and Grant U22A2002in part by the National Key Research and Development Program of China under Grant 2020YFA0711301+2 种基金in part by the Leading Project of Minzu University of China under Grant 2023QNYL23in part by the Key Research and Development Project of Nantong(Special Project for Prospective Technology Innovation)under Grant GZ2024002in part by the Suzhou Science and Technology Project,and in part by the FAW Jiefang Automotive Co.,Ltd.
文摘Satellite and terrestrial cellular networks can be integrated together to achieve extended broad-band coverage for,e.g.,maritime communication sce-narios,in the upcoming sixth-generation(6G)era.To counter spectrum scarcity,collaborative spectrum sharing is considered for hybrid satellite-terrestrial networks(HSTNs)in this paper.With only slowly-varying large-scale channel state information(CSI),joint power and channel allocation is implemented for terrestrial mobile terminals(MTs)which share the same frequency band with the satellite MTs oppor-tunistically.Specially,strict quality service assurance is adopted for terrestrial MTs under the constraint of leakage interference to satellite MTs.With the tar-get of maximizing both the number of served terres-trial MTs and the average sum transmission rate,a double-target spectrum sharing problem is formulated.To solve the complicated mixed integer programming(MIP)problem efficiently,user-centric channel pools are introduced.Simulations demonstrate that the proposed spectrum sharing scheme could achieve a significant performance gain for the HSTN.
基金Supported by the National Natural Science Foundation of China(No.81371667,No.31271073)
文摘In recent years, organic-inorganic hybrid nanocarriers are explored for effective drug delivery and preferable disease treatments. In this study, using 5-fluorouracil(5-FU)as electronegative model drug, a new type of organic-inorganic hybrid drug delivery system(LDH/HA-PEG/5-FU)was conceived and manufactured by the adsorption of PEGylated hyaluronic acid(HA-PEG)on the surface of layered double hydroxide(LDH, prepared via hydrothermal method)and the intercalation of 5-FU in the interlamination of LDH via ion exchange strategy. The drug loading amount of LDH/HA-PEG/5-FU achieved as high as 34.2%. LDH, LDH/5-FU and LDH/HA-PEG/5-FU were characterized by FT-IR, XRD, TGA, laser particle size analyzer and SEM. With the benefit of p Hdegradable feature of LDH and enzyme-degradable feature of HA, LDH/HA-PEG/5-FU showed p H-degradable and enzyme-degradable capacity in in vitro drug release. Moreover, the drug carrier LDH/HA-PEG contained biocompatible PEG and tumor-targeted HA, resulting in lower cytotoxicity and better endocytosis compared with LDH in vitro. It was suggested that the organic-inorganic hybrid drug delivery system, which was endowed with the properties of controlled release, low toxicity and tumor-targeting delivery for ameliorative cancer therapy, was advisable and might be applied further to fulfill other treatments.
基金supported by the Natural Science Research Project of Colleges and Universities in Anhui Province (No.KJ2021A0479)the Science Research Program of Anhui University of Finance and Economics (No.ACKYC22082)。
文摘We present a gain adaptive tuning method for fiber Raman amplifier(FRA) using two-stage neural networks(NNs) and double weights updates. After training the connection weights of two-stage NNs separately in training phase, the connection weights of the unified NN are updated again in verification phase according to error between the predicted and target gains to eliminate the inherent error of the NNs. The simulation results show that the mean of root mean square error(RMSE) and maximum error of gains are 0.131 d B and 0.281 d B, respectively. It shows that the method can realize adaptive adjustment function of FRA gain with high accuracy.
基金supported by the Labex IGO project(n°ANR11-LABX-0016-01)funded by the "Investissements d'Avenir" French Government program,managed by the French National Research Agency(ANR)+1 种基金the context of the IHU-Cesti project(EXT173947) which received French government financial support managed by the National Research Agency via the "Investment Into The Future program" ANR-10-IBHU-005supported by Nantes Metropole and Region Pays de la Loire. C.L.was supported by Fondation Progreffe
文摘The CRISPR/Cas9 system has been tailored to a revolutionary genetic tool because of its remarkable simplicity and efficacy.While complex genome editing in the mouse since the 1990 s has been dominated by the use of embryonic stem（ES） cells,CRISPR/Cas9 now offers a versatile and fast approach to precisely modify virtually any DNA regions directly in mouse zygotes.Yet,this relative simplicity does not preclude a conscientious preparatory work that is often neglected when initiating a project.Here,we describe the key steps leading to successful generation of a double knockout（KO） mouse by simultaneously targeting two homolog genes,Tmem176 a and Tmem176 b,which are located in the same genomic locus.Additionally,we show that similar efficiency can be obtained in a mixed genetic background or directly in the C57BL/6 inbred strain.Thus,presented as a detailed case study that should be helpful to the non-specialists,we focus on the genotyping strategy to anticipate the various possibilities.
基金supported by National Natural Science Foundation of China (No.10975026)
文摘Interaction between high-intensity pulsed ion beam （HIPIB） and a double-layer target with titanium film on top of aluminum substrate was simulated. The two-dimensional nonlinear thermal conduction equations, with the deposited energy in the target taken as source term, were derived and solved by finite differential method. As a result, the two-dimensional spatial and temporal evolution profiles of temperature were obtained for a titanium/aluminum double-layer target irradiated by a pulse of HIPIB. The effects of ion beam current density on the phase state of the target materials near the film and substrate interface were analyzed. Both titanium and aluminum were melted near the interface after a shot when the ion beam current density fell in the range of 100 A/cm2 to 200 A/cm2.
基金Supported by Shandong Swine Industry Technology System and Science and Technology Planning Program for Basic Research in Qingdao City(12-1-4-14-jch)
文摘Gene targeting technology is an important means to investigate gene functions, but its efficiency of gene targeting is very low, especially for somatic cell targeting. Artificially induced double-strand breaks （DSB） and triplex forming oligonucleotide （TFO） are currently developed methods to improve the targeting efficiency. This paper summarized the basic principles, design ideas and application in gene targeting efficiency improvement of these two methods, analyzed and com- pared their characteristics, and finally proposed prospects for their future development.
基金Supported by Demonstration and Promotion Project of Shanghai Municipal Committee of Agriculture[HNKT(2015)2-7]Jiangsu Agricultural"Three New Engineering"Project[SXGC(2017)208]Jiangsu Agricultural Science and Technology Innovation Fund[CX(15)1029]~~
文摘In order to develop green good, reduce the use of chemical fertilizer and pesticide, realize ＂double reduction＂ and synergism, improve safety and quality of products, protect the ecological environment and promote the healthy and steady development of strawberry industry, the goals and key technology of fertilizer-pesticide ＂double reduction＂ and synergism for greenhouse strawberry are summed up in this paper targeting at the current situation of strawberry production and combined with the new technology achievements at home and abroad. The key technology includes six items： healthy seedling cultivating technique, soil improvement and continuous cropping obstacle treatment technique, agricultural ecological prevention tech- nique, physicochemical trap of pest control technique, biological control technique and low residual risk chemical control technique.
文摘The purpose of initial orbit determination,especially in the case of angles-only data for observation,is to obtain an initial estimate that is close enough to the true orbit to enable subsequent precision orbit determination processing to be successful.However,the classical angles-only initial orbit determination methods cannot deal with the observation data whose Earth-central angle is larger than 360°.In this paper,an improved double r-iteration initial orbit determination method to deal with the above case is presented to monitor geosynchronous Earth orbit objects for a spacebased surveillance system.Simulation results indicate that the improved double r-iteration method is feasible,and the accuracy of the obtained initial orbit meets the requirements of re-acquiring the object.
基金This research work was partially funded by the Chiang Mai University.
文摘In the most recent decades,a major number of image encryption plans have been proposed.The vast majority of these plans reached a highsecurity level;however,their moderate speeds because of their complicated processes made them of no use in real-time applications.Inspired by this,we propose another efficient and rapid image encryption plan dependent on the Trigonometric chaotic guide.In contrast to the most of current plans,we utilize this basic map to create just a couple of arbitrary rows and columns.Moreover,to additionally speed up,we raise the processing unit from the pixel level to the row/column level.The security of the new plot is accomplished through a substitution permutation network,where we apply a circular shift of rows and columns to break the solid connection of neighboring pixels.At that point,we join the XOR operation with modulo function to cover the pixels values and forestall any leaking of data.High-security tests and simulation analyses are carried out to exhibit that the scheme is very secure and exceptionally quick for real-time image processing at 80 fps(frames per second).
文摘Breast cancer is a significant global concern,with limited effective treatment options.Therefore,therapies with high efficacy and low complications,unlike the existing chemotherapies,are urgently required.To address this issue,advances have been made in therapies targeting molecular pathways related to the murine double minute 2 protooncogene(MDM2)-tumor proteinp53(TP53)interaction.This review aims to investigate the efficacy of MDM2 inhibition in restoring TP53 activity in breast cancer cells,as evidenced by clinical studies,reviews,and trials.TP53 is a tumor suppressor and MDM2 facilitates proteasomal degradation of TP53.MDM2 and TP53 activity is tightly regulated.However,cancerous breast cells overexpress MDM2 through five hypothesized mechanisms.Consequently,TP53 levels decrease with increased tumor cell proliferation.Three strategies have been identified for controlling MDM2 upregulation in cells with wild-type or mutated TP53.MDM2 inhibitors(MDM2i)are administered in combination with existing chemotherapies to reduce their effects on healthy cells.Few clinical and preclinical studies have been conducted using MDM2i,which necessitates high-quality clinical trials to support their therapeutic potential in breast cancer therapy.
文摘In this paper, the shortages of Wigner-Ville Distribution(WVD) double-linearity in extracting the Doppler parameters of moving-targets are discussed, especially in multi-point moving-target imaging processing based on the spectrum characteristics of moving-target echo signals in Synthetic Aperture Radar (SAR) imaging processing and the properties of WVD. Combined with the characteristics of Continuous Wavelet Transform (CWT), the responsibility and advantages of CWT in multi-point moving-target Doppler parameter extraction are analyzed. Finally a kind of multi-point moving-target Doppler parameter extracting algorithm based on CWT are developed, and the computer stimulating results demonstrate the correctness of the algorithm.
文摘气候问题日趋严重,“双碳”目标引起学者的广泛关注。为分析当前国内外“双碳”领域研究前沿与热点差异,本文利用科学计量技术,针对知网和Web of Science核心数据集自2009—2022年有关“双碳”目标的文献进行时空维度内文献分布特征分析和研究前沿与热点动态演化分析。结果表明:“双碳”领域正处于研究浪潮阶段,自2020年后“双碳”领域发文量呈爆发式增长,研究力量集中于西方发达国家。研究侧重点存在差异,国外“双碳”领域从生物质和太阳能利用等技术角度展开研究,国内则侧重政策治理层面,低碳转型成为研究热点。本文提出“双碳”领域未来发展方向将聚焦于低碳技术和模式创新,以创新助推低碳转型。
