A novel composite of aptamer-based Ti_(3)C_(2)T_(x)and molecularly imprinted polymer(Apt-Ti_(3)C_(2)T_(x)-MIP)with double recognition property was prepared for sensitive electrochemical detection of ofloxacin(OFL).Fir...A novel composite of aptamer-based Ti_(3)C_(2)T_(x)and molecularly imprinted polymer(Apt-Ti_(3)C_(2)T_(x)-MIP)with double recognition property was prepared for sensitive electrochemical detection of ofloxacin(OFL).First,Ti_(3)C_(2)T_(x)@SiO_(2)was synthesized using multi-layer Ti_(3)C_(2)T_(x)and tetraethyl orthosilicate(TEOS)in alkaline conditions.Amino Ti_(3)C_(2)T_(x)@SiO_(2)was then prepared through Ti_(3)C_(2)T_(x)@SiO_(2)in the presence of(3-aminopropyl)triethoxysilane.Carboxyl OFL aptamers were then grafted onto amino Ti_(3)C_(2)T_(x)@SiO_(2)to prepare aptamer-modified Ti_(3)C_(2)T_(x)@SiO_(2).A novel composite of Apt-Ti_(3)C_(2)T_(x)-MIP was prepared with sol–gel method in the presence of Ti_(3)C_(2)T_(x)@SiO_(2)-Apt-OFL and TEOS.The Apt-Ti_(3)C_(2)T_(x)-MIP sensor exhibited double recognition for OFL because of aptamer specificity and imprinted binding sites.The electrochemical response of Apt-Ti_(3)C_(2)T_(x)-MIP sensor for OFL was 2.29 and 2.75 times that of Apt-Ti_(3)C_(2)T_(x)-NIP and Ti_(3)C_(2)T_(x)-MIP sensors,respectively,demonstrating the double recognition of Apt-Ti_(3)C_(2)T_(x)-MIP for OFL.The Apt-Ti_(3)C_(2)T_(x)-MIP sensor with double recognition displayed a linear peak current to OFL centration from 0.025 to 10μM with a detection limit of 10 nM.The Apt-Ti_(3)C_(2)T_(x)-MIP sensor had excellent selectivity for OFL and has been successfully applied to the detection of OFL in a wastewater sample.展开更多
基金supported by the National Natural Science Foundation of China(21507041,21677060)the Zhejiang Province Public Welfare Technology Application Research Project(LGF21B050004)+1 种基金Scientific Research Fund of Zhejiang Provincial Education Department(Y202352065)Jiaxing Municipal Public Welfare Research Project(2022AY10015)。
文摘A novel composite of aptamer-based Ti_(3)C_(2)T_(x)and molecularly imprinted polymer(Apt-Ti_(3)C_(2)T_(x)-MIP)with double recognition property was prepared for sensitive electrochemical detection of ofloxacin(OFL).First,Ti_(3)C_(2)T_(x)@SiO_(2)was synthesized using multi-layer Ti_(3)C_(2)T_(x)and tetraethyl orthosilicate(TEOS)in alkaline conditions.Amino Ti_(3)C_(2)T_(x)@SiO_(2)was then prepared through Ti_(3)C_(2)T_(x)@SiO_(2)in the presence of(3-aminopropyl)triethoxysilane.Carboxyl OFL aptamers were then grafted onto amino Ti_(3)C_(2)T_(x)@SiO_(2)to prepare aptamer-modified Ti_(3)C_(2)T_(x)@SiO_(2).A novel composite of Apt-Ti_(3)C_(2)T_(x)-MIP was prepared with sol–gel method in the presence of Ti_(3)C_(2)T_(x)@SiO_(2)-Apt-OFL and TEOS.The Apt-Ti_(3)C_(2)T_(x)-MIP sensor exhibited double recognition for OFL because of aptamer specificity and imprinted binding sites.The electrochemical response of Apt-Ti_(3)C_(2)T_(x)-MIP sensor for OFL was 2.29 and 2.75 times that of Apt-Ti_(3)C_(2)T_(x)-NIP and Ti_(3)C_(2)T_(x)-MIP sensors,respectively,demonstrating the double recognition of Apt-Ti_(3)C_(2)T_(x)-MIP for OFL.The Apt-Ti_(3)C_(2)T_(x)-MIP sensor with double recognition displayed a linear peak current to OFL centration from 0.025 to 10μM with a detection limit of 10 nM.The Apt-Ti_(3)C_(2)T_(x)-MIP sensor had excellent selectivity for OFL and has been successfully applied to the detection of OFL in a wastewater sample.
