Objective:To explore and verify the mechanism of Gan Dou Ling in improving liver fibrosis in Wilson disease(WD)by network pharmacology and copper loaded mice experiments.Methods:The main chemical components and corres...Objective:To explore and verify the mechanism of Gan Dou Ling in improving liver fibrosis in Wilson disease(WD)by network pharmacology and copper loaded mice experiments.Methods:The main chemical components and corresponding gene targets of each drug in Gan Dou Ling were obtained by using TCMSP database.The database of gene mutation and disease related gene was searched through the GeneCards database,DrugBank database,PharmGKB database and the DisGeNET database.After the intersection of drug and disease target genes.The STRING website was used to analyze the protein-protein interaction degree of target genes,and import the data to Cytoscape software 38.2 to analyze protein interaction network.The GO databases and KEGG databases were obtained in R language for enrichment analysis.On this basis,Masson staining were used to observe the degree of liver fibrosis in copper loaded mouse model,and the results of network pharmacological analysis were verified by Western Blot(WB).Results:A total of 108 drug disease intersection genes were analyzed by network pharmacology.Through PPI network analysis,JUN was found to be the key genes.The enrichment analysis of KEGG pathway showed that MAPK signal pathway was the important potential target pathways.Animal experiments showed that Gan Dou Ling could reduce liver fibrosis and inhibit the phosphorylation of P38,JNK and C-JUN in copper loaded mice.Conclusion:Gan Dou Ling may achieve the effect of treating WD liver fibrosis by inhibiting P38/JNK signaling pathway.展开更多
THE ancient town of Huangyao located in Zhaoping County,Hezhou City,south China’s Guangxi Zhuang Autonomous Region,is renown for its time-honored Douchi processing techniques.Douchi is a kind of popular Chinese food ...THE ancient town of Huangyao located in Zhaoping County,Hezhou City,south China’s Guangxi Zhuang Autonomous Region,is renown for its time-honored Douchi processing techniques.Douchi is a kind of popular Chinese food made from fermented and salted black beans,which is commonly used as a condiment or seasoning for a variety of dishes.The history of Huangyao’s Douchi processing techniques can be traced back to the late Yuan Dynasty(1271-1368)and the early Ming Dynasty(1368-1644).According to a historical record of the Qing Dynasty(1644-1911),“Huangyao is famous for its Douchi and has no rivals.”展开更多
At 19:45 Beijing time on November 5,a LM-3B/Yuanzheng 1 launch vehicle lifted off from the Xichang Satellite Launch Center,putting the first two Bei Dou 3 satellites into orbit after the separation of the rocket upper...At 19:45 Beijing time on November 5,a LM-3B/Yuanzheng 1 launch vehicle lifted off from the Xichang Satellite Launch Center,putting the first two Bei Dou 3 satellites into orbit after the separation of the rocket upper stage and the satellites.The launch opens up a new era for the global networking of the Bei Dou Navigation Satellite system(Bei Dou system),which is the final step of the"three-step development strategy"of the system.展开更多
The octagonal star pattern painted pottery dou vessel of the Dawenkou Culture has long been an important representative of prehistoric painted pottery art because of its unique shape and decorative patterns.Scholars h...The octagonal star pattern painted pottery dou vessel of the Dawenkou Culture has long been an important representative of prehistoric painted pottery art because of its unique shape and decorative patterns.Scholars have put forward many explanations for the meaning of the octagonal star pattern.Among them,the"sun worship theory"is the most common.But most related studies infer it only by the similar shape of the image.This paper takes the octagonal star pattern painted pottery dou vessel of the Dawenkou Culture collected in Shandong Museum as the research object.On the basis of sorting out the existing research views,it re-discusses the symbolic meaning of the octagonal star pattern by combining archaeological discoveries,the function of the artifacts and related remains of the same period.This study thinks that directly equating the decorative pattern with the sun pattern may lead to over-interpretation;it is more likely to be a symbolic image formed by the method of"forming symbolic images through the observation of objects",which comprehensively reflects the prehistoric ancestors'understanding of natural environment,agricultural production and group identity.The octagonal star pattern may have a conceptual connection with natural beliefs such as sun worship,but it is not their direct image correspondence,and should be understood in a more complex cultural context.This paper aims to reflect on the applicability of different interpretation approaches in the study of prehistoric decorative patterns through this specific case,and provide a more cautious research perspective for the interpretation of the meaning of related images.展开更多
Some writers take no prisoners.In his first book translated into English,satirist Diao Dou wows us with a heady stew of literary allusion,controlled anger and human frustration.
Yin Aihong,aged 45,has been working on Hanzheng Street since she was a teenager.Between 8 p.m.and 11 p.m.,she has a frequent change of costumes during her livestreaming on Douyin,the Chinese version of TikTok.
Some writers take no prisoners.In his first book translated into English,sati-rist Diao Dou wows us with a heady stew of literary allusion,controlled anger and human frustration.
基金supported by the National Natural Science Foundation of China(No.81973825)the Natural Science Research Foundation of Anhui Province Universities(No.KJ2020A0436)。
文摘Objective:To explore and verify the mechanism of Gan Dou Ling in improving liver fibrosis in Wilson disease(WD)by network pharmacology and copper loaded mice experiments.Methods:The main chemical components and corresponding gene targets of each drug in Gan Dou Ling were obtained by using TCMSP database.The database of gene mutation and disease related gene was searched through the GeneCards database,DrugBank database,PharmGKB database and the DisGeNET database.After the intersection of drug and disease target genes.The STRING website was used to analyze the protein-protein interaction degree of target genes,and import the data to Cytoscape software 38.2 to analyze protein interaction network.The GO databases and KEGG databases were obtained in R language for enrichment analysis.On this basis,Masson staining were used to observe the degree of liver fibrosis in copper loaded mouse model,and the results of network pharmacological analysis were verified by Western Blot(WB).Results:A total of 108 drug disease intersection genes were analyzed by network pharmacology.Through PPI network analysis,JUN was found to be the key genes.The enrichment analysis of KEGG pathway showed that MAPK signal pathway was the important potential target pathways.Animal experiments showed that Gan Dou Ling could reduce liver fibrosis and inhibit the phosphorylation of P38,JNK and C-JUN in copper loaded mice.Conclusion:Gan Dou Ling may achieve the effect of treating WD liver fibrosis by inhibiting P38/JNK signaling pathway.
文摘THE ancient town of Huangyao located in Zhaoping County,Hezhou City,south China’s Guangxi Zhuang Autonomous Region,is renown for its time-honored Douchi processing techniques.Douchi is a kind of popular Chinese food made from fermented and salted black beans,which is commonly used as a condiment or seasoning for a variety of dishes.The history of Huangyao’s Douchi processing techniques can be traced back to the late Yuan Dynasty(1271-1368)and the early Ming Dynasty(1368-1644).According to a historical record of the Qing Dynasty(1644-1911),“Huangyao is famous for its Douchi and has no rivals.”
文摘At 19:45 Beijing time on November 5,a LM-3B/Yuanzheng 1 launch vehicle lifted off from the Xichang Satellite Launch Center,putting the first two Bei Dou 3 satellites into orbit after the separation of the rocket upper stage and the satellites.The launch opens up a new era for the global networking of the Bei Dou Navigation Satellite system(Bei Dou system),which is the final step of the"three-step development strategy"of the system.
文摘The octagonal star pattern painted pottery dou vessel of the Dawenkou Culture has long been an important representative of prehistoric painted pottery art because of its unique shape and decorative patterns.Scholars have put forward many explanations for the meaning of the octagonal star pattern.Among them,the"sun worship theory"is the most common.But most related studies infer it only by the similar shape of the image.This paper takes the octagonal star pattern painted pottery dou vessel of the Dawenkou Culture collected in Shandong Museum as the research object.On the basis of sorting out the existing research views,it re-discusses the symbolic meaning of the octagonal star pattern by combining archaeological discoveries,the function of the artifacts and related remains of the same period.This study thinks that directly equating the decorative pattern with the sun pattern may lead to over-interpretation;it is more likely to be a symbolic image formed by the method of"forming symbolic images through the observation of objects",which comprehensively reflects the prehistoric ancestors'understanding of natural environment,agricultural production and group identity.The octagonal star pattern may have a conceptual connection with natural beliefs such as sun worship,but it is not their direct image correspondence,and should be understood in a more complex cultural context.This paper aims to reflect on the applicability of different interpretation approaches in the study of prehistoric decorative patterns through this specific case,and provide a more cautious research perspective for the interpretation of the meaning of related images.
文摘Some writers take no prisoners.In his first book translated into English,satirist Diao Dou wows us with a heady stew of literary allusion,controlled anger and human frustration.
文摘Yin Aihong,aged 45,has been working on Hanzheng Street since she was a teenager.Between 8 p.m.and 11 p.m.,she has a frequent change of costumes during her livestreaming on Douyin,the Chinese version of TikTok.
文摘Some writers take no prisoners.In his first book translated into English,sati-rist Diao Dou wows us with a heady stew of literary allusion,controlled anger and human frustration.
