Objective: The purpose of this study was to develop a novel colorimetric system for direct detection of hydrogen sulfide on the tongue dorsum from subjects with complaints of halitosis. Method: An assay system compris...Objective: The purpose of this study was to develop a novel colorimetric system for direct detection of hydrogen sulfide on the tongue dorsum from subjects with complaints of halitosis. Method: An assay system comprised of a cotton swab impregnated with zinc sulfate, attached chromatography paper, stabilizing solution, and coloring reagents for the methylene blue technique was developed. Using this system we investigated on the visualization of the hydrogen sulfide on the solid phase and possibility for evaluating the origin of oral malodor on the tongue dorsum. Results: The assay system made possible visualization of a linear curve ranging from 5.3 ng to 85 ng of hydrogen sulfide, with low reagent consumption and miniaturization. However, the sensitivity was different for gaseous and fluid samples. The biofilm of the tongue dorsum from subjects with complaints of halitosis showed different shades of methylene blue depending on the concentration of hydrogen sulfide. A positive correlation was found between hydrogen sulfide on the tongue dorsum and the concentration of VSC hydrogen sulfide in mouth breath as measured using an Oral Chroma semiconductor gas sensor. Conclusion: The results of this study suggest that the novel assay system can be used as a visual warning sensor for halitosis by measuring hydrogen sulfide on the tongue dorsum.展开更多
Background There are few reports of microsurgical treatment of dorsum sellae meningiomas--which, because of location, size, and differences in growth direction, clinical presentations, degree of surgical difficulty, h...Background There are few reports of microsurgical treatment of dorsum sellae meningiomas--which, because of location, size, and differences in growth direction, clinical presentations, degree of surgical difficulty, have varied posttreatment sequelae. In pursuit of an optimal microsurgical treatment option for dorsum sellae meningioma patients, we performed a retrospective analysis of eight microsurgery-treated patients in our set up. Methods Clinical data of eight microsurgery-treated dorsum sellae meningioma patients were analyzed. Dorsum sellae meningiomas were classified into 2 types based on tumor location, size, and direction of growth. Type Ⅰ tumors （dorsum sellae-inferior third ventricle type, four cases） were resected by craniotomy via the frontotemporal or orbitozygomatic approach. Type Ⅱ tumors （dorsum sellae-third ventricle type, 4 cases） were resected by frontal craniotomy via the transcallosal-interforniceal approach. Results Complete tumor resection was achieved in all the eight patients. In Type Ⅰ tumor patients, the only postoperative complication was oculomotor nerve palsy. In Type Ⅱ tumor patients, the postoperative complications included hyperthermia, electrolyte imbalances, endocrinologic disturbances, and hydrocephalus. The mean follow-up was 2.1 years. Four patients returned to normal life and found a job, two were able to live independently, one required assistance, while one died. Conclusions Dorsum sellae meningioma surgery is challenging, and resection of Type Ⅱ tumors is more difficult than Type Ⅰ tumors. The selection of a suitable microsurgical approach based on tumor type, and the active treatment of postoperative complications are important means of increasing therapeutic efficacy.展开更多
文摘Objective: The purpose of this study was to develop a novel colorimetric system for direct detection of hydrogen sulfide on the tongue dorsum from subjects with complaints of halitosis. Method: An assay system comprised of a cotton swab impregnated with zinc sulfate, attached chromatography paper, stabilizing solution, and coloring reagents for the methylene blue technique was developed. Using this system we investigated on the visualization of the hydrogen sulfide on the solid phase and possibility for evaluating the origin of oral malodor on the tongue dorsum. Results: The assay system made possible visualization of a linear curve ranging from 5.3 ng to 85 ng of hydrogen sulfide, with low reagent consumption and miniaturization. However, the sensitivity was different for gaseous and fluid samples. The biofilm of the tongue dorsum from subjects with complaints of halitosis showed different shades of methylene blue depending on the concentration of hydrogen sulfide. A positive correlation was found between hydrogen sulfide on the tongue dorsum and the concentration of VSC hydrogen sulfide in mouth breath as measured using an Oral Chroma semiconductor gas sensor. Conclusion: The results of this study suggest that the novel assay system can be used as a visual warning sensor for halitosis by measuring hydrogen sulfide on the tongue dorsum.
文摘Background There are few reports of microsurgical treatment of dorsum sellae meningiomas--which, because of location, size, and differences in growth direction, clinical presentations, degree of surgical difficulty, have varied posttreatment sequelae. In pursuit of an optimal microsurgical treatment option for dorsum sellae meningioma patients, we performed a retrospective analysis of eight microsurgery-treated patients in our set up. Methods Clinical data of eight microsurgery-treated dorsum sellae meningioma patients were analyzed. Dorsum sellae meningiomas were classified into 2 types based on tumor location, size, and direction of growth. Type Ⅰ tumors （dorsum sellae-inferior third ventricle type, four cases） were resected by craniotomy via the frontotemporal or orbitozygomatic approach. Type Ⅱ tumors （dorsum sellae-third ventricle type, 4 cases） were resected by frontal craniotomy via the transcallosal-interforniceal approach. Results Complete tumor resection was achieved in all the eight patients. In Type Ⅰ tumor patients, the only postoperative complication was oculomotor nerve palsy. In Type Ⅱ tumor patients, the postoperative complications included hyperthermia, electrolyte imbalances, endocrinologic disturbances, and hydrocephalus. The mean follow-up was 2.1 years. Four patients returned to normal life and found a job, two were able to live independently, one required assistance, while one died. Conclusions Dorsum sellae meningioma surgery is challenging, and resection of Type Ⅱ tumors is more difficult than Type Ⅰ tumors. The selection of a suitable microsurgical approach based on tumor type, and the active treatment of postoperative complications are important means of increasing therapeutic efficacy.
