Bud dormancy represents a critical adaptation mechanism in perennial fruit plants,enabling survival during unfavorable environmental conditions.While this adaptive strategy is essential for plant survival and reproduc...Bud dormancy represents a critical adaptation mechanism in perennial fruit plants,enabling survival during unfavorable environmental conditions.While this adaptive strategy is essential for plant survival and reproduction,its molecular mechanisms remain incompletely understood.This study investigated two grape transcription factors:EARLY BUD BREAK(VvEBB)and SHORT VEGETATIVE PHASE 4(VvSVP4).Through heterologous transformation in poplar,the research demonstrated that VvSVP4 functions as a negative regulator of bud break,while VvEBB serves as a positive regulator.Transcriptome analysis revealed significant enrichment of plant hormone signaling pathways particularly ABA,IAA,and CK in plants overexpressing VvSVP4(VvSVP4-OE)and VvEBB(VvEBB-OE)compared to controls.Endogenous hormone level changes correlated positively with transcriptome data.During endodormancy,VvSVP4 directly and positively regulated the ABA receptor gene Vv PYL9 expression,maintaining bud dormancy.Conversely,during ecodormancy,rapid upregulation of VvEBB negatively regulated the expression of sucrose nonfermenting 1-related protein kinase 2 gene(Vv SAPK2),facilitating dormancy release.This study provides comprehensive insights into how VvSVP4 and VvEBB genes regulate dormancy and bud break through cell cycle regulation and multiple hormone signaling pathways.展开更多
A study was conducted to describe the progression of bud dormancy in 1-year-old apple （Malus x domestica Borkh） shoots grown at two contrasting climatic conditions （Belgium, temperate and Ethiopia, tropics）. The e...A study was conducted to describe the progression of bud dormancy in 1-year-old apple （Malus x domestica Borkh） shoots grown at two contrasting climatic conditions （Belgium, temperate and Ethiopia, tropics）. The experiment was carried out on ＂Golden＂ and ＂Gala＂ cultivars for two consecutive years （2010/2011 and 2011/2012）. Moreover, a validation experiment was conducted on ＂MM106＂ apple rootstock during 2010/2011 only in Ethiopia. Variations in inverse of time to 50% budburst were interpreted in terms of evolution of growth capacity of the buds. Despite differences observed with chilling accumulation later in winter or early in spring, depending on environments, depth of endodormancy intensity during winter can be summarized as follows： buds from pruned shoots were less endodormant than terminal buds of the intact shoots and terminal buds were more endodormant than the dormancy intensity of upper buds of the disbudded shoots, suggesting proximal buds can grow more readily than does terminal ones. Our results provide evidence for the existence of a considerably strong paradormancy inhibition by distal shoot parts and buds, which was more pronounced in Ethiopia than in Belgium, highlighting the importance of designing and applying appropriate pruning and dormancy avoidance strategies in mild-winter climates. Finally, as still there is knowledge gap on bud dormancy progression and its control mechanism especially under mild climates, our study highlights the need for further in-depth research using biological and biochemical tests.展开更多
In grapevine,previous studies have suggested that,gibberellin(GA)inhibits bud break before dormancy release while enhanced after dormancy release;the capacity of GA accumulation shows a trend of first inhibition and t...In grapevine,previous studies have suggested that,gibberellin(GA)inhibits bud break before dormancy release while enhanced after dormancy release;the capacity of GA accumulation shows a trend of first inhibition and then upregulation.However,the regulatory mechanism of GA metabolism genes expression is as yet unclear during the process of dormancy release.In this study,we further validated the effect of GA3 and hydrogen cyanamide(HC)on bud break of‘Red Globe’grape,confirmed inhibition and promotion effect,respectively.Restricted GA biosynthetic genes'expression and enhanced GA catabolic gene's expression were observed in the early stage after HC treatment,while opposite expression trend showed in the late stage.VvSVP1,a MADS-box transcription factor gene,was downregulated in the late stage,which might play an important role in regulating GA metabolism genes'expression.It was shown that,VvSVP1 could bind to the promoter regions of GA biosynthetic gene VvGA20ox6 and catabolic gene VvGA2ox3,negatively and positively regulated the corresponding genes'expression,respectively;the contents of GAs related to GA20ox were significantly reduced in the grape callus overexpressed VvSVP1,while the ratio of GAs related to GA2ox were significantly increased.Taken together,VvSVP1 can regulate the endogenous GAs level by manipulating the expression of GA metabolism genes before dormant bud break induced by HC.Our findings may provide some new theoretical insights for the study of bud dormancy regulation in the perennial woody fruit trees.展开更多
基金supported by the China Agriculture Research System of MOF and MARA(CARS-29)the Xinjiang Uygur Autonomous Region Tianchi Talent Introduction Program,Chinathe Key R&D Technology Commissioner Projects in Hainan Province,China(ZDYF2024KJTPY008)。
文摘Bud dormancy represents a critical adaptation mechanism in perennial fruit plants,enabling survival during unfavorable environmental conditions.While this adaptive strategy is essential for plant survival and reproduction,its molecular mechanisms remain incompletely understood.This study investigated two grape transcription factors:EARLY BUD BREAK(VvEBB)and SHORT VEGETATIVE PHASE 4(VvSVP4).Through heterologous transformation in poplar,the research demonstrated that VvSVP4 functions as a negative regulator of bud break,while VvEBB serves as a positive regulator.Transcriptome analysis revealed significant enrichment of plant hormone signaling pathways particularly ABA,IAA,and CK in plants overexpressing VvSVP4(VvSVP4-OE)and VvEBB(VvEBB-OE)compared to controls.Endogenous hormone level changes correlated positively with transcriptome data.During endodormancy,VvSVP4 directly and positively regulated the ABA receptor gene Vv PYL9 expression,maintaining bud dormancy.Conversely,during ecodormancy,rapid upregulation of VvEBB negatively regulated the expression of sucrose nonfermenting 1-related protein kinase 2 gene(Vv SAPK2),facilitating dormancy release.This study provides comprehensive insights into how VvSVP4 and VvEBB genes regulate dormancy and bud break through cell cycle regulation and multiple hormone signaling pathways.
文摘A study was conducted to describe the progression of bud dormancy in 1-year-old apple （Malus x domestica Borkh） shoots grown at two contrasting climatic conditions （Belgium, temperate and Ethiopia, tropics）. The experiment was carried out on ＂Golden＂ and ＂Gala＂ cultivars for two consecutive years （2010/2011 and 2011/2012）. Moreover, a validation experiment was conducted on ＂MM106＂ apple rootstock during 2010/2011 only in Ethiopia. Variations in inverse of time to 50% budburst were interpreted in terms of evolution of growth capacity of the buds. Despite differences observed with chilling accumulation later in winter or early in spring, depending on environments, depth of endodormancy intensity during winter can be summarized as follows： buds from pruned shoots were less endodormant than terminal buds of the intact shoots and terminal buds were more endodormant than the dormancy intensity of upper buds of the disbudded shoots, suggesting proximal buds can grow more readily than does terminal ones. Our results provide evidence for the existence of a considerably strong paradormancy inhibition by distal shoot parts and buds, which was more pronounced in Ethiopia than in Belgium, highlighting the importance of designing and applying appropriate pruning and dormancy avoidance strategies in mild-winter climates. Finally, as still there is knowledge gap on bud dormancy progression and its control mechanism especially under mild climates, our study highlights the need for further in-depth research using biological and biochemical tests.
基金supported by the National Natural Science Foundation of China(Grant No.32172514).
文摘In grapevine,previous studies have suggested that,gibberellin(GA)inhibits bud break before dormancy release while enhanced after dormancy release;the capacity of GA accumulation shows a trend of first inhibition and then upregulation.However,the regulatory mechanism of GA metabolism genes expression is as yet unclear during the process of dormancy release.In this study,we further validated the effect of GA3 and hydrogen cyanamide(HC)on bud break of‘Red Globe’grape,confirmed inhibition and promotion effect,respectively.Restricted GA biosynthetic genes'expression and enhanced GA catabolic gene's expression were observed in the early stage after HC treatment,while opposite expression trend showed in the late stage.VvSVP1,a MADS-box transcription factor gene,was downregulated in the late stage,which might play an important role in regulating GA metabolism genes'expression.It was shown that,VvSVP1 could bind to the promoter regions of GA biosynthetic gene VvGA20ox6 and catabolic gene VvGA2ox3,negatively and positively regulated the corresponding genes'expression,respectively;the contents of GAs related to GA20ox were significantly reduced in the grape callus overexpressed VvSVP1,while the ratio of GAs related to GA2ox were significantly increased.Taken together,VvSVP1 can regulate the endogenous GAs level by manipulating the expression of GA metabolism genes before dormant bud break induced by HC.Our findings may provide some new theoretical insights for the study of bud dormancy regulation in the perennial woody fruit trees.
