This study aimed to identify the physiological mechanisms enabling low-N-tolerant maize cultivar to maintain higher photosynthesis and yield under low-N,low-light,and combined stress.In a three-year field trial of low...This study aimed to identify the physiological mechanisms enabling low-N-tolerant maize cultivar to maintain higher photosynthesis and yield under low-N,low-light,and combined stress.In a three-year field trial of low-N-tolerant and low-N-sensitive maize cultivars under two N fertilization(normal N:240 kg N ha^(−1);low-N:150 kg N ha^(−1))and two light conditions(normal light;low-light:35%light reduction),the tolerant cultivar showed higher net photosynthetic rate than the sensitive one.Random Forest analysis and Structural Equation Modeling identified PSI donor-side limitation(elevated Y_(ND))as the key photosynthetic constraint.The tolerant cultivar maintained higher D1 and PsaA protein levels and preferentially allocated photosynthetic N to electron transport.This strategy reduced Y_(ND)and sustained photosystem stability,thus improving carboxylation efficiency and resulting in higher photosynthesis.展开更多
以番茄品种辽园多丽为试验材料,研究适当遮光对番茄越夏栽培过程中常遭遇的高温强光(HH, high temperature and highlight)对叶片光系统Ⅱ及光合机构恢复能力的影响。结果表明:HH和HH下适当遮光(HL, HH with proper shading)均导致番茄...以番茄品种辽园多丽为试验材料,研究适当遮光对番茄越夏栽培过程中常遭遇的高温强光(HH, high temperature and highlight)对叶片光系统Ⅱ及光合机构恢复能力的影响。结果表明:HH和HH下适当遮光(HL, HH with proper shading)均导致番茄幼苗叶片的光合作用效率显著降低,非气孔性限制起主导作用。番茄幼苗叶片PSⅡ反应中心活性降低,PSⅡ电子传递受阻,PsbO和PsbA基因相对表达量降低,即HH和HL对番茄幼苗叶片的主要作用位点为PSⅡ供体侧放氧复合体(OEC)和PSⅡ受体侧QA向QB的传递过程,其原因主要与OEC功能的破坏及D1蛋白的降解有关。HH胁迫下PsbA基因相对表达量降低幅度大于PsbO,并且标准化OJIP曲线上2 ms处相对可变荧光(VJ)的增加幅度大于0.3 ms处相对可变荧光(VK);HL胁迫下PsbA和PsbO基因相对表达量均有降低,但幅度小于HH。说明HH胁迫对番茄幼苗PSⅡ受体侧的伤害程度大于供体侧,HL胁迫下番茄PSⅡ供体侧和受体侧均有损伤,但伤害程度较弱。经12 h恢复后,HH和HL的光合作用能力均有不同程度的提高,适当遮光提高的程度较多,且PSⅡ供体侧和受体侧基本可以恢复到对照水平。此外,HH和HL下番茄幼苗PSⅡ反应中心对光能的捕获及利用能力降低,单位面积有活性反应中心的数量和吸收光能用于电子传递的能量比例均有不同程度的下降,PSⅡ反应中心吸收光能多以热能形式进行耗散。HH和HL经12 h恢复后均有不同程度的增加,HL增加的幅度较明显。因此,在番茄设施越夏栽培的种植和推广时,可以考虑通过遮光网适当遮光的方式缓解高温强光对番茄的产量和品质造成的影响。展开更多
We sought to evaluate the efficacy and effects of low-dose tacrolimus （FK506） to recipients with living donor liver transplantation （LDLT）. A total of 66 patients who underwent LDLT between 2001 and 2007 were enro...We sought to evaluate the efficacy and effects of low-dose tacrolimus （FK506） to recipients with living donor liver transplantation （LDLT）. A total of 66 patients who underwent LDLT between 2001 and 2007 were enrolled in this study. According to different doses of tacrolimus, the recipients were randomly divided into two groups： the low-dose tacrolimus group （group A） and the normal-dose tacrolimus group （group B）. The blood concentration of tacrolimus and its side effects including infection, hyperglycemia, hypertension, high blood creatinine and jaundice were monitored once a week at the perioperative period, and once a month thereafter. Besides, the survival rates of the recipients were analyzed at the 1and 3-year time point after operation. Among these patients, no significant acute rejection was detected after LDLT. The incidences of infection, hyperglycemia, liver dysfunction and renal impairment in group A were markedly lower than those in group B. However, no significant differences were detected in the incidence of hypertension between the two groups. Moreover, the recipients in each group had a similar survival rate according to the results of 1and 3-year follow-up. The incidence of side effects that associated with tacrolimus positively correlated with tacrolimus blood concentration. In conclusion, long-term and low-dose administration of tacrolimus is a safe and effective treatment for LDLT recipients.展开更多
基金supported by the Key Program of Natural Science Foundation of Sichuan Province(2022NSFSC0013)National Key Research and Development Program of China(2022YFD1901603,2023YFD2301902).
文摘This study aimed to identify the physiological mechanisms enabling low-N-tolerant maize cultivar to maintain higher photosynthesis and yield under low-N,low-light,and combined stress.In a three-year field trial of low-N-tolerant and low-N-sensitive maize cultivars under two N fertilization(normal N:240 kg N ha^(−1);low-N:150 kg N ha^(−1))and two light conditions(normal light;low-light:35%light reduction),the tolerant cultivar showed higher net photosynthetic rate than the sensitive one.Random Forest analysis and Structural Equation Modeling identified PSI donor-side limitation(elevated Y_(ND))as the key photosynthetic constraint.The tolerant cultivar maintained higher D1 and PsaA protein levels and preferentially allocated photosynthetic N to electron transport.This strategy reduced Y_(ND)and sustained photosystem stability,thus improving carboxylation efficiency and resulting in higher photosynthesis.
文摘以番茄品种辽园多丽为试验材料,研究适当遮光对番茄越夏栽培过程中常遭遇的高温强光(HH, high temperature and highlight)对叶片光系统Ⅱ及光合机构恢复能力的影响。结果表明:HH和HH下适当遮光(HL, HH with proper shading)均导致番茄幼苗叶片的光合作用效率显著降低,非气孔性限制起主导作用。番茄幼苗叶片PSⅡ反应中心活性降低,PSⅡ电子传递受阻,PsbO和PsbA基因相对表达量降低,即HH和HL对番茄幼苗叶片的主要作用位点为PSⅡ供体侧放氧复合体(OEC)和PSⅡ受体侧QA向QB的传递过程,其原因主要与OEC功能的破坏及D1蛋白的降解有关。HH胁迫下PsbA基因相对表达量降低幅度大于PsbO,并且标准化OJIP曲线上2 ms处相对可变荧光(VJ)的增加幅度大于0.3 ms处相对可变荧光(VK);HL胁迫下PsbA和PsbO基因相对表达量均有降低,但幅度小于HH。说明HH胁迫对番茄幼苗PSⅡ受体侧的伤害程度大于供体侧,HL胁迫下番茄PSⅡ供体侧和受体侧均有损伤,但伤害程度较弱。经12 h恢复后,HH和HL的光合作用能力均有不同程度的提高,适当遮光提高的程度较多,且PSⅡ供体侧和受体侧基本可以恢复到对照水平。此外,HH和HL下番茄幼苗PSⅡ反应中心对光能的捕获及利用能力降低,单位面积有活性反应中心的数量和吸收光能用于电子传递的能量比例均有不同程度的下降,PSⅡ反应中心吸收光能多以热能形式进行耗散。HH和HL经12 h恢复后均有不同程度的增加,HL增加的幅度较明显。因此,在番茄设施越夏栽培的种植和推广时,可以考虑通过遮光网适当遮光的方式缓解高温强光对番茄的产量和品质造成的影响。
文摘We sought to evaluate the efficacy and effects of low-dose tacrolimus （FK506） to recipients with living donor liver transplantation （LDLT）. A total of 66 patients who underwent LDLT between 2001 and 2007 were enrolled in this study. According to different doses of tacrolimus, the recipients were randomly divided into two groups： the low-dose tacrolimus group （group A） and the normal-dose tacrolimus group （group B）. The blood concentration of tacrolimus and its side effects including infection, hyperglycemia, hypertension, high blood creatinine and jaundice were monitored once a week at the perioperative period, and once a month thereafter. Besides, the survival rates of the recipients were analyzed at the 1and 3-year time point after operation. Among these patients, no significant acute rejection was detected after LDLT. The incidences of infection, hyperglycemia, liver dysfunction and renal impairment in group A were markedly lower than those in group B. However, no significant differences were detected in the incidence of hypertension between the two groups. Moreover, the recipients in each group had a similar survival rate according to the results of 1and 3-year follow-up. The incidence of side effects that associated with tacrolimus positively correlated with tacrolimus blood concentration. In conclusion, long-term and low-dose administration of tacrolimus is a safe and effective treatment for LDLT recipients.
