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双目立体视觉与特征匹配下机械臂位姿估计
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作者 朱晶晶 翟佳佳 王福忠 《机械设计与制造》 北大核心 2026年第2期238-243,共6页
在高维流形空间中,机械臂运动会呈现出奇异位姿,这种位姿运转产生的振动会传递到摄像头上,导致摄像头发生位移变化,使得采集到的图像中关键位姿角点出现尺度动态变化特性。若不考虑机械臂关键位姿角点与当前状态之间的几何约束一致性,... 在高维流形空间中,机械臂运动会呈现出奇异位姿,这种位姿运转产生的振动会传递到摄像头上,导致摄像头发生位移变化,使得采集到的图像中关键位姿角点出现尺度动态变化特性。若不考虑机械臂关键位姿角点与当前状态之间的几何约束一致性,会导致位姿估计出现偏差。为此,提出双目立体视觉与特征匹配下机械臂位姿估计方法。通过双目系统外参数校正摄像头标定偏差,引入高斯卷积核辨识校正后双目系统采集到的二维机械臂视差图的多尺度空间;引入具有尺度不变特性的DoG算子检测多尺度空间下的关键位姿角点,并利用欧式距离对关键位姿特征角点匹配,有效解决了因尺度动态变化导致的角点匹配失败问题。将特征匹配得到的角点信息与机械臂的运动姿态相结合,利用UKF算法实时更新和估计机械臂的位姿状态,确保关键位姿角点与当前状态之间的几何约束一致性,实现机械臂位姿的有效估计,减少因特征匹配不准确带来的位姿估计偏差。实验表明,所提方法特征匹配精确度较高,位姿输出结果与机械臂实际位姿之间的一致性较高,能够有效地满足机械臂工业应用中对精度和可靠性的要求。 展开更多
关键词 几何变换矩阵 DoG算子 特征点提取 SIFT算法 UKF算法
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Blind Tibetan Opens Horizon to Wide World of Opportunities
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作者 Palden Nyima(Text/Photos) Daqiong(Text/Photos) 《China's Tibet》 2026年第1期56-58,共3页
When Dachung Wochen,a 35-year-old blind Tibetan,walks in the bustling streets of Lhasa,capital of the Xizang Autonomous Region,with his guide dog Fuju,people are amazed by their tacit cooperation.Fuju helps his master... When Dachung Wochen,a 35-year-old blind Tibetan,walks in the bustling streets of Lhasa,capital of the Xizang Autonomous Region,with his guide dog Fuju,people are amazed by their tacit cooperation.Fuju helps his master navigate every obstacle on the road.After spending only a year with him,he understands most of his commands in Tibetan.Dachung Wochen's journey with the four-year-old canine began in April 2024.After 28 days of professional training at the China Guide Dog Training Center in Dalian,Liaoning Province,Dachung,as he is called by friends and family,took Fuju to Xizang by air and rail. 展开更多
关键词 BLIND LHASA TIBETAN Dachung Wochen Xizang Autonomous Region guide dog Fuju China Guide Dog Training Center
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Biocompatibility and healing patterns in experimentally induced canine tibial fractures using Pedicle screw- Rod external fixation
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作者 Mohammad Mahdi Gooran Ramin Mazaheri-Khameneh +1 位作者 Seyed Mohammad Hashemi-Asl Rahim Hobbenaghi 《Animal Models and Experimental Medicine》 2026年第1期31-40,共10页
Background:Biological osteosynthesis preserves blood supply and promotes rapid healing by aligning fracture fragments without direct surgical exposure.Pedicle screws are primarily designed for internal fixation in spi... Background:Biological osteosynthesis preserves blood supply and promotes rapid healing by aligning fracture fragments without direct surgical exposure.Pedicle screws are primarily designed for internal fixation in spinal procedures.A key objec-tive of many orthopedic studies is to assess the biocompatibility of implants with bone and adjacent soft tissue.This study aims to evaluate the biocompatibility and effects of the Pedicle screw-Rod configuration as a novel external fixation method in canine tibial osteotomy.Methods:With ethics approval,eight healthy,intact male dogs,aged 10-12 months and weighing between 20 and 22 kg,underwent a minimally invasive medial tibial approach for surgical fixation of tibial osteotomy using a Pedicle screw-Rod configu-ration.Postoperative evaluations included ultrasound assessments at the osteotomy site and histological evaluations at the bone-screw interface.Results:B-mode ultrasound evaluation indicated healing progress at all osteotomy sites.The color Doppler examination revealed an initial increase in signals in the sur-rounding soft tissue during the first 4 weeks post-operation,followed by a decrease in signals within the adjacent soft tissue between the 5th and 8th weeks.During this latter period,the signals were primarily concentrated on the bone surface and the callus.The bone-screw interface at various screw sites exhibited similar histological changes,indicating effective integration of the newly formed woven bone into the screw threads.Conclusions:Fixation of non-articular tibial osteotomy with Pedicle screw-Rod con-figuration resulted in secondary bone healing,characterized by abundant callus for-mation and neovascularization.This implant demonstrated favorable biocompatibility with bone and surrounding soft tissue,without significant complications. 展开更多
关键词 dog fracture NEOVASCULARIZATION OSTEOINTEGRATION Pedicle screw-Rod TIBIA
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Genetic Evolution Analysis of Rabies Virus in Jiangsu Province,China
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作者 Minghui Zhang Yuanfang Qin +8 位作者 Na zhang Yuqiao Liu Jun Yang Xiaonuo Xu Pengcheng Yu Shuqing Liu Qian Liu Xiaoyan Tao Wuyang Zhu 《Biomedical and Environmental Sciences》 2026年第2期245-250,共6页
Rabies is a zoonotic disease with an estimated global mortality of 59,000 people annually and a burden of more than 3.7 million disability-adjusted life years(DALYs)that is caused by a neurotropic lyssavirus[1].Dogs a... Rabies is a zoonotic disease with an estimated global mortality of 59,000 people annually and a burden of more than 3.7 million disability-adjusted life years(DALYs)that is caused by a neurotropic lyssavirus[1].Dogs are the primary source of human rabies,as more than 95%of human cases can be traced to dogs[2,3].China faces a substantial burden of rabies,having endured three major human rabies epidemics,which occurred in the 1950s,1981,and 2007[4].Implementation of various prevention and control measures has decreased the number of human rabies cases from 3,300 in 2007 to 167 in 2024.In China. 展开更多
关键词 rabies virus Jiangsu province neurotropic lyssavirus dogs neurotropic lyssavirus prevention control measures China genetic evolution zoonotic disease
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Sex with Strangers Dogging English Parks 被引量:1
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作者 Jason Hopps 王颖 《当代外语研究》 2003年第11期8-9,共2页
英语词汇如何获得新的词义,本文似乎作了清晰的演绎。本文标题中的dogging,显然是“一直跟踪;尾随”的意思,如:A stranger then is still dogging us.(一个陌生人仍在跟踪我们。)但是,文中出现了另一个dogging,作者对它的释义是:The ter... 英语词汇如何获得新的词义,本文似乎作了清晰的演绎。本文标题中的dogging,显然是“一直跟踪;尾随”的意思,如:A stranger then is still dogging us.(一个陌生人仍在跟踪我们。)但是,文中出现了另一个dogging,作者对它的释义是:The term“dogging”apparently comes from those who claim only to be“tak-ing the dog for a walk”-but are actually on the prowl(徘徊)for somethingmore.而这里的something more,即指标题中的Sex with Strangers。文章不乏此类隐晦的表达,另如:…and online message boards list the best parks and car-parks to watch people express themselves.其中的express themselves的真实含义,就值得我们三思玩味。 展开更多
关键词 Sex with Strangers dogging English Parks
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基于球面DOG小波框架的骨料形状重构研究
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作者 李景哲 高鹏 +3 位作者 詹炳根 胡焱博 沙慧玲 余其俊 《工程力学》 北大核心 2025年第6期195-202,共8页
基于高斯差分小波(Difference of Gaussians,DOG)向球面上拓展产生的球面DOG小波函数,提出了基于球面DOG小波框架重构骨料形状的新方法。为探讨该方法的优势,采用该方法和传统球谐重构法,对采用三维扫描仪获取机制砂的表面点云数据进行... 基于高斯差分小波(Difference of Gaussians,DOG)向球面上拓展产生的球面DOG小波函数,提出了基于球面DOG小波框架重构骨料形状的新方法。为探讨该方法的优势,采用该方法和传统球谐重构法,对采用三维扫描仪获取机制砂的表面点云数据进行重构,分析了球面DOG小波框架方法的参数设置,以及准确性。结果显示,随着球面DOG小波框架qmax的增大,重构骨料的形貌逐渐接近真实形态。且当qmax达到4时,支撑集足够小的球面DOG小波可有效避免高阶次球谐级数重构多棱角骨料时由于振铃效应导致的重构精度下降问题。 展开更多
关键词 球面DOG小波框架 球谐函数 三维扫描 机制砂 颗粒形状
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三七休眠调控因子DOG基因家族的全基因组鉴定及表达模式
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作者 黄敏 李朝林 +4 位作者 葛娜 贾金山 王清艳 张金燕 陈军文 《中草药》 北大核心 2025年第8期2898-2913,共16页
目的研究三七Panax notoginseng DOG(PnDOG)基因家族结构特征和表达模式及其在三七种子后熟休眠和萌发的作用机制。方法基于三七的全基因组和转录组数据,利用生物信息手段分析PnDOG家族的理化性质、基因结构、系统发育关系、保守结构域... 目的研究三七Panax notoginseng DOG(PnDOG)基因家族结构特征和表达模式及其在三七种子后熟休眠和萌发的作用机制。方法基于三七的全基因组和转录组数据,利用生物信息手段分析PnDOG家族的理化性质、基因结构、系统发育关系、保守结构域、顺式作用元件和表达模式;探讨了外施赤霉素(gibberellins,GA)和脱落酸(abscisic acid,ABA)激素对三七种子的影响,以及PnDOG基因家族对这些外源激素的响应机制。结果PnDOG基因家族包含16个成员,不均匀地分布在8条染色体上。PnDOG基因都含有外显子,编码216~514个氨基酸,均为亲水性蛋白,亚细胞定位预测显示均位于细胞核中。系统进化将PnDOG基因分为3个亚组,不同亚组成员的基因结构存在差异。共线性分析显示,串联重复是PnDOG基因扩增的主要驱动因素。启动子顺式作用元件分析显示,PnDOG基因的启动子区含有大量激素响应、胁迫响应以及少量胚发育响应等元件。基因本体(geneontology,GO)和京都基因与基因组百科全书(Kyoto encyclopedia of genes and genomes,KEGG)分析显示,PnDOG基因可能在非生物应激和激素诱导中起作用。基因表达分析和qRT-PCR分析显示,PnDOG基因在三七不同组织中均有表达,并具有明显的组织特异性。部分PnDOG基因在三七种子不同后熟阶段和激素处理下的表达量均存在显著差异。发现PnDOG1和PnDOG14在种子中高表达且受激素强烈诱导,其表达量随后熟时间的增加逐渐下降,推测PnDOG1和PnDOG14是调控三七种子后熟休眠的关键基因。结论大多数PnDOG基因的高表达可能与三七种子的后熟休眠形成有关,在后熟过程中通过响应激素信号调控种子休眠解除。 展开更多
关键词 三七 种子休眠 DOG基因家族 表达模式 外源激素
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种子休眠及其调控机制研究进展
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作者 段若昕 陈盈盈 +1 位作者 林金星 李瑞丽 《生物技术通报》 北大核心 2025年第12期16-26,共11页
种子休眠是指种子在适宜环境条件下暂时无法萌发的现象,这一特性可以防止作物的收获前萌发。在林业中,研究种子休眠有利于森林培育,因此具有重要应用价值。研究表明植物激素脱落酸(abscisic acid, ABA)诱导和维持种子休眠,赤霉素(gibber... 种子休眠是指种子在适宜环境条件下暂时无法萌发的现象,这一特性可以防止作物的收获前萌发。在林业中,研究种子休眠有利于森林培育,因此具有重要应用价值。研究表明植物激素脱落酸(abscisic acid, ABA)诱导和维持种子休眠,赤霉素(gibberellin, GA)解除休眠,二者以拮抗作用的形式调控种子的休眠和萌发。尽管已经有一些在激素调控种子休眠和萌发方面的研究,但是缺少对种子休眠分子机制的系统总结。因此,本文综述了种子休眠的类型、光温信号对种子休眠的影响,重点阐述了ABA、GA及激素间相互作用对种子休眠的调控机制,并对休眠特异性调控因子(如DOG1、DOG18、Sdr4)及表观遗传调控机制进行了归纳总结,以期加深对种子休眠复杂调控网络的理解,为植物性状的改良和分子设计育种提供理论参考。 展开更多
关键词 种子休眠 脱落酸 赤霉素 DOG1 表观遗传调控
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基于SIFT算法的计算机图形自适应匹配研究 被引量:1
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作者 郭林 沈东义 毛火明 《电子设计工程》 2025年第15期38-43,共6页
为了提升计算机图形自适应匹配方法匹配效率、匹配准确度,实现计算机图形特征的精准匹配,该文提出基于SIFT算法的计算机图形自适应匹配方法。通过高斯卷积核构建尺度空间,在尺度空间引入DoG极值算子,提取计算机图形位于尺度空间的极值... 为了提升计算机图形自适应匹配方法匹配效率、匹配准确度,实现计算机图形特征的精准匹配,该文提出基于SIFT算法的计算机图形自适应匹配方法。通过高斯卷积核构建尺度空间,在尺度空间引入DoG极值算子,提取计算机图形位于尺度空间的极值特征点,计算准确插值并以梯度幅值为基础,确定计算机图形极值特征点的主要方向,将特征点所在相邻区间分割成小区间,获取不同小区间的方位与向量特征描述因子,作为提取到的计算机图形极值特征点;自适应寻找最优化的距离比阈值,结合位置方向欧氏距离(POED)函数对所获取特征描述因子进行匹配,实现计算机图形的自适应匹配。实验表明,该方法可以实现对计算机图形自适应匹配,匹配用时较少,匹配准确度较高;对有噪声干扰的计算机图形依然可实现匹配,具有较高鲁棒性。 展开更多
关键词 SIFT算法 计算机图形 自适应匹配 高斯卷积核 DoG算子 POED函数
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The love and wisdom of a mother dog
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作者 周露云 《疯狂英语(新读写)》 2025年第5期54-55,77,78,共4页
A veterinary(兽医的)clinic in Türkiye recently shared the touching story of a stray dog who carried her unconscious puppy in her mouth and left it at the clinic door,as if begging for help.If not for the viral vi... A veterinary(兽医的)clinic in Türkiye recently shared the touching story of a stray dog who carried her unconscious puppy in her mouth and left it at the clinic door,as if begging for help.If not for the viral video footage captured by CCTV cameras,most people would probably think this story too heart⁃warming to be true.A veterinary clinic in the Adnan Kahveci neighborhood of Beylikdüzü,in Türkiye's Istanbul Province,recently reported one of its most incredible cases.On January 13,an animal lover brought a young puppy he had found on the street,claiming it was the only survivor of a stray's litter.The clinic rushed to treat the puppy only to then find that its mother showed up on the doorstep with another unconscious puppy in her mouth.A technician noticed the desperate animal and rushed to tend to her offspring,which suffered from hypothermia(体温过低)and had a very low heart rate. 展开更多
关键词 her unconscious puppy veterinary clinic animal lover unconscious puppy viral video footage cctv camerasmost stray dog mother dog
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When the earth shook,our world changed
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作者 宋鹭 《疯狂英语(新悦读)》 2025年第10期4-8,76,共6页
I never thought much about earthquakes until that morning.I was making coffee in my kitchen,still half asleep.My dog Max was acting strangely,whining(哀鸣)and running around in circles.Then my coffee cup started shaki... I never thought much about earthquakes until that morning.I was making coffee in my kitchen,still half asleep.My dog Max was acting strangely,whining(哀鸣)and running around in circles.Then my coffee cup started shaking.At first I thought I was just tired,but soon the whole kitchen was moving.The floor felt like jelly under my feet. 展开更多
关键词 SHAKING coffee cup FLOOR earthquake KITCHEN DOG
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A Special Friendship
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作者 张超 张素芬 《中学生英语》 2025年第13期6-6,共1页
One sunny day,a little dog with the name Max met a little cat named Lily in the backyard.Max:Hi,Lily!I'm looking for something interesting to do.Do you want to play with me?Lily:Sure,Max!But how 1 can we play?Max:... One sunny day,a little dog with the name Max met a little cat named Lily in the backyard.Max:Hi,Lily!I'm looking for something interesting to do.Do you want to play with me?Lily:Sure,Max!But how 1 can we play?Max:How about hide-and-seek?Lily:Sound②great!You count first,and I'll go hide. 展开更多
关键词 PLAY FRIENDSHIP cat BACKYARD hide seek DOG
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Dogs
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作者 黄刚 张超(指导) 《中学生英语》 2025年第13期7-7,共1页
I love dogs because they are so friendly and lovely.With bright eyes,dogs can see things in the dark.Their noses are very sensitive,and this helps them find things easily.My pet dog is named Dahu.He is white with long... I love dogs because they are so friendly and lovely.With bright eyes,dogs can see things in the dark.Their noses are very sensitive,and this helps them find things easily.My pet dog is named Dahu.He is white with long ears.Every day when I come back home from school,he runs to me happily and wags his tail.We often play games together.He likes to chase a ball,and I throw the ball far,then he runs quickly to bring it back. 展开更多
关键词 friendly PETS chase balland sensitivity DOGS APPEARANCE find things noses
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The most expensive dog in the world
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作者 贺黎玮 《疯狂英语(新读写)》 2025年第8期54-55,79,共3页
Activity 1 Think about the following questions and write down your answers before reading the text.1.What factors might contribute to the high price of a rare dog?2.How do you think the ownership of such an expensive ... Activity 1 Think about the following questions and write down your answers before reading the text.1.What factors might contribute to the high price of a rare dog?2.How do you think the ownership of such an expensive dog might impact the owner's lifestyle or responsibilities? 展开更多
关键词 price factors ownership impact responsibilities rare dogs THINK LIFESTYLE
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Why My Favourite Pet Is Special
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作者 张盼 陈传光 《中学生英语》 2025年第14期6-6,共1页
My favourite pet is a lovely dog.I bought him last year in the pet market.He has long ears,a long body and brown fur.He is very small.He weights①about one kilogram.He is very active.He wants to catch everything.Every... My favourite pet is a lovely dog.I bought him last year in the pet market.He has long ears,a long body and brown fur.He is very small.He weights①about one kilogram.He is very active.He wants to catch everything.Every day he runs here and there.But if you want him to do something,he will be a very good boy. 展开更多
关键词 brown fur active weight long ears small long body FAVOURITE DOG
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Magnamosis for rectal reconstruction in canines
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作者 Shi-Qi Liu Hong-Ke Zhang +3 位作者 Yi Lv Xiang-Hua Xu Yu-Feng Li Dong-Wen Quan 《World Journal of Gastrointestinal Surgery》 2025年第2期227-236,共10页
BACKGROUND The magnamosis,a minimal invasive,suture-free procedure,has been used for digestive tract or vessel reconstruction,such as gastrointestinal anastomosis,bilioenteric anastomosis,and coronary artery bypass.Al... BACKGROUND The magnamosis,a minimal invasive,suture-free procedure,has been used for digestive tract or vessel reconstruction,such as gastrointestinal anastomosis,bilioenteric anastomosis,and coronary artery bypass.Although some case reports have demonstrated the potential of magnamosis for the treatment of congenital rectal atresia(RA),they cannot provide strong evidence for its widespread application.AIM To assess the feasibility and safety of magnamosis in treating RA in dogs as compared to suturing anastomosis.The findings of this study can be beneficial in guiding the clinical application of magnamosis.METHODS Thirty-six dogs were randomly assigned to the magnamosis group(n=18)and the suturing anastomosis group(n=18).The rectum was freed laparoscopically in all dogs.In the magnamosis group,rectal anastomosis was performed using a pair of magnetic rings,while the suturing anastomosis group underwent a straight-sighted end-to-end rectal anastomosis with 4-0 absorbable sutures.The anastomosis time was recorded,and abdominal plain film examination was performed to locate the magnets until they were expelled postoperatively.Specimens of the anastomosis were evaluated at one month,three months,and six months after surgery.RESULTS The mean time for rectal anastomosis was significantly shorter in the magnamosis group(12.22±2.78 minutes)than the suturing anastomosis group(18.11±1.68 minutes).There was one incidence of anastomotic bleeding in the suturing anastomosis group,whereas no complication was recorded in the magnamosis group.The magnets were discharged post-surgery in 7.17±1.30 days in all the dogs.The histopathological examination revealed a smoother healing of anastomotic mucosa in the magnamosis group as compared to that in the suturing anastomosis group.Moreover,the fiber alignment was also more natural in the magnamosis group with minimal inflammation.CONCLUSION Rectal reconstruction using magnamosis is a feasible,safe,and effective alternative to suturing anastomosis in dogs,with the added benefit of faster and more natural healing of the anastomosis. 展开更多
关键词 Rectal reconstruction CANINES Magnamosis DOGS
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善意时刻
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作者 Joseph J.Mazzella 《疯狂英语(新读写)》 2025年第6期34-36,76,共4页
简单的善举不仅温暖了他人,也让施助者感受到内心的充盈与喜悦,既彰显出爱的力量,更传递出分享的真谛。1 I have no idea why some stores make shelves so high that only a professional basketball player can reach them,but they d... 简单的善举不仅温暖了他人,也让施助者感受到内心的充盈与喜悦,既彰显出爱的力量,更传递出分享的真谛。1 I have no idea why some stores make shelves so high that only a professional basketball player can reach them,but they do.I experienced one of them just the other day.I had just picked up some toothpaste and deodorant and was heading to the dog food section when I took a short cut through the toy section. 展开更多
关键词 TOYS toy section STORES short cut dog food shelves
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WORLD NEWS
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《ChinAfrica》 2025年第10期12-13,共2页
ITALY A crescent moon rises at sunset behind Monte Camicia in Teramo on 3 September US A dog rides the waves with its trainer during the 20th annual Surf Dog Surf-a-Thon in Del Mar,California,on 7 September.
关键词 surf dog surf thon monte camicia WAVES teramo crescent moon sunset
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Head-Body Guided Deep Learning Framework for Dog Breed Recognition
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作者 Noman Khan Afnan +1 位作者 Mi Young Lee Jakyoung Min 《Computers, Materials & Continua》 2025年第11期2935-2958,共24页
Fine-grained dog breed classification presents significant challenges due to subtle inter-class differences,pose variations,and intra-class diversity.To address these complexities and limitations of traditional handcr... Fine-grained dog breed classification presents significant challenges due to subtle inter-class differences,pose variations,and intra-class diversity.To address these complexities and limitations of traditional handcrafted approaches,a novel and efficient two-stageDeep Learning(DL)framework tailored for robust fine-grained classification is proposed.In the first stage,a lightweight object detector,YOLO v8N(You Only Look Once Version 8 Nano),is finetuned to localize both the head and full body of the dog from each image.In the second stage,a dual-stream Vision Transformer(ViT)architecture independently processes the detected head and body regions,enabling the extraction of region-specific,complementary features.This dual-path approach improves feature discriminability by capturing localized cues that are vital for distinguishing visually similar breeds.The proposed framework introduces several key innovations:(1)a modular and lightweight head–body detection pipeline that balances accuracy with computational efficiency,(2)a region-awareViT model that leverages spatial attention for enhanced fine-grained recognition,and(3)a training scheme incorporating advanced augmentations and structured supervision to maximize generalization.These contributions collectively enhancemodel performancewhilemaintaining deployment efficiency.Extensive experiments conducted on the Tsinghua Dogs dataset validate the effectiveness of the approach.The model achieves an accuracy of 90.04%,outperforming existing State-of-the-Art(SOTA)methods across all key evaluation metrics.Furthermore,statistical significance testing confirms the robustness of the observed improvements over multiple baselines.The proposed method presents an effective solution for breed recognition tasks and shows strong potential for broader applications,including pet surveillance,veterinary diagnostics,and cross-species classification.Notably,it achieved an accuracy of 96.85% on the Oxford-IIIT Pet dataset,demonstrating its robustness across different species and breeds. 展开更多
关键词 Animal science computer vision dog breed deep learning RECOGNITION
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Molecular Identification of Echinococcus spp. and Other Taeniid Tapeworms Using Next-Generation Sequence Analysis of PCR Amplified 18s rRNA Gene
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作者 Rasmi Abu-Helu George Kokaly +3 位作者 Sajeda Nojoum Imad Matouk Murad Ibrahim Ibrahim Abbasi 《American Journal of Molecular Biology》 2025年第1期75-87,共13页
Cystic echinococcosis (CE) is a prevalent zoonotic disease caused by Echinococcus granulosus, with a cosmopolitan distribution. The parasite is transmitted cyclically between canines and numerous intermediate herbivor... Cystic echinococcosis (CE) is a prevalent zoonotic disease caused by Echinococcus granulosus, with a cosmopolitan distribution. The parasite is transmitted cyclically between canines and numerous intermediate herbivorous livestock animals. Also, other Taeniid tapeworms could infect domestic dogs and they pose significant veterinary and public health concerns worldwide. This study aimed to develop a sensitive molecular method for detecting Echinococcus spp. DNA in dog fecal samples using next-generation sequencing (NGS). A set of PCR primers targeting conserved regions of Taeniid tapeworms’ 18s rRNA genes was designed and tested for amplifying genomic DNA from various tapeworm species. The PCR system demonstrated high sensitivity, amplifying DNA from all tested tapeworm species, with differences observed in amplified band sizes. The primers were adapted for NGS analysis by adding forward and reverse adapters, enabling the sequencing of amplified DNA fragments. Application of the developed PCR system to dog fecal samples collected from Yatta town, Palestine, revealed the presence of E. granulosus DNA in five out of 50 samples. NGS analysis confirmed the specificity of the amplified DNA fragments, showing 98% - 99% similarity with the 18s rDNA gene of E. granulosus. This study demonstrates the utility of NGS-based molecular methods for accurate and sensitive detection of Echinococcus spp. in dog fecal samples, providing valuable insights for epidemiological surveillance and control programs of echinococcosis in endemic regions. 展开更多
关键词 Cystic Echinococcosis Taeniid Tapeworms Next-Generation Sequencing Molecular Detection Dog Fecal Samples
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