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RNA-seq转录组测序分析青少年MDD患者外周血lncRNA、miRNA、mRNA表达差异
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作者 姜洁 任真奎 +6 位作者 刘志国 张珺杰 尹利君 赵强 涂贵兰 孙建超 何军 《贵州医药》 2025年第2期171-176,共6页
目的RNA-seq转录组测序分析青少年重度抑郁症(major depression disease,MDD)患者外周血差异表达的lncRNA、miRNA、mRNA,为阐明青少年MDD发病机制提供理论补充。方法随机收集样本库4例青少年女性MDD患者与4例正常人对照外周血,TRizol试... 目的RNA-seq转录组测序分析青少年重度抑郁症(major depression disease,MDD)患者外周血差异表达的lncRNA、miRNA、mRNA,为阐明青少年MDD发病机制提供理论补充。方法随机收集样本库4例青少年女性MDD患者与4例正常人对照外周血,TRizol试剂处理后,提取RNA后进行RNA-seq转录组测序,根据P<0.05且|Log2FC|>1计算两组样本的表达差异,绘制差异lncRNA、miRNA、mRNA热图与火山图。通过TargetScan数据库可能预测差异miRNA调控的mRNA,并进行GO功能与KEGG信号通路聚类。结果MDD组和对照组两组间满足P<0.05且|Log2FC|>1差异条件的MDD组中1636个lncRNA上调和825个lncRNA下调,42个miRNAs上调和30个miRNAs下调,2469个mRNA上调和1398个mRNA下调。靶基因主要富集的GO功能:生物学过程(BP)、分子功能(MF)、细胞组分(CC)三个方面。靶基因主要富集的KEGG信号通路:差异表达的lncRNA多富集在Toll-like receptor信号通路、TNF信号通路、Salmonella intection和NF-kappa B信号通路等;差异表达的miRNA多富集在VEGF信号通路、Vasopressin-regulated water reabsorption、Vascular smooth muscle contraction、MAPK信号通路等;差异表达的mRNA靶基因多富集在泛素介导的蛋白质水解、T细胞受体信号通路和MAPK信号通路等。结论本研究结果提供了青少年女性MDD外周血中lncRNA、miRNA、mRNA表达变化的一般情况与可能调控的功能与通路,可能有助于阐明青少年MDD的潜在发病机制。 展开更多
关键词 青少年mdd RNA-seq转录组测序 LncRNA micoRNA mRNA
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Construction of a Maritime Knowledge Graph Using GraphRAG for Entity and Relationship Extraction from Maritime Documents 被引量:1
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作者 Yi Han Tao Yang +2 位作者 Meng Yuan Pinghua Hu Chen Li 《Journal of Computer and Communications》 2025年第2期68-93,共26页
In the international shipping industry, digital intelligence transformation has become essential, with both governments and enterprises actively working to integrate diverse datasets. The domain of maritime and shippi... In the international shipping industry, digital intelligence transformation has become essential, with both governments and enterprises actively working to integrate diverse datasets. The domain of maritime and shipping is characterized by a vast array of document types, filled with complex, large-scale, and often chaotic knowledge and relationships. Effectively managing these documents is crucial for developing a Large Language Model (LLM) in the maritime domain, enabling practitioners to access and leverage valuable information. A Knowledge Graph (KG) offers a state-of-the-art solution for enhancing knowledge retrieval, providing more accurate responses and enabling context-aware reasoning. This paper presents a framework for utilizing maritime and shipping documents to construct a knowledge graph using GraphRAG, a hybrid tool combining graph-based retrieval and generation capabilities. The extraction of entities and relationships from these documents and the KG construction process are detailed. Furthermore, the KG is integrated with an LLM to develop a Q&A system, demonstrating that the system significantly improves answer accuracy compared to traditional LLMs. Additionally, the KG construction process is up to 50% faster than conventional LLM-based approaches, underscoring the efficiency of our method. This study provides a promising approach to digital intelligence in shipping, advancing knowledge accessibility and decision-making. 展开更多
关键词 Maritime Knowledge Graph GraphRAG Entity and Relationship Extraction document Management
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Malicious Document Detection Based on GGE Visualization
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作者 Youhe Wang Yi Sun +1 位作者 Yujie Li Chuanqi Zhou 《Computers, Materials & Continua》 SCIE EI 2025年第1期1233-1254,共22页
With the development of anti-virus technology,malicious documents have gradually become the main pathway of Advanced Persistent Threat(APT)attacks,therefore,the development of effective malicious document classifiers ... With the development of anti-virus technology,malicious documents have gradually become the main pathway of Advanced Persistent Threat(APT)attacks,therefore,the development of effective malicious document classifiers has become particularly urgent.Currently,detection methods based on document structure and behavioral features encounter challenges in feature engineering,these methods not only have limited accuracy,but also consume large resources,and usually can only detect documents in specific formats,which lacks versatility and adaptability.To address such problems,this paper proposes a novel malicious document detection method-visualizing documents as GGE images(Grayscale,Grayscale matrix,Entropy).The GGE method visualizes the original byte sequence of the malicious document as a grayscale image,the information entropy sequence of the document as an entropy image,and at the same time,the grayscale level co-occurrence matrix and the texture and spatial information stored in it are converted into grayscale matrix image,and fuses the three types of images to get the GGE color image.The Convolutional Block Attention Module-EfficientNet-B0(CBAM-EfficientNet-B0)model is then used for classification,combining transfer learning and applying the pre-trained model on the ImageNet dataset to the feature extraction process of GGE images.As shown in the experimental results,the GGE method has superior performance compared with other methods,which is suitable for detecting malicious documents in different formats,and achieves an accuracy of 99.44%and 97.39%on Portable Document Format(PDF)and office datasets,respectively,and consumes less time during the detection process,which can be effectively applied to the task of detecting malicious documents in real-time. 展开更多
关键词 Malicious document VISUALIZATION EfficientNet-B0 convolutional block attention module GGE image
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Chinese Documentaries
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《China Today》 2025年第1期73-73,共1页
This video series is the first experimental psychology documentary made in China.It focuses on analyzing professional theories to raise people’s general understanding of basic psychology.By combining innovative audio... This video series is the first experimental psychology documentary made in China.It focuses on analyzing professional theories to raise people’s general understanding of basic psychology.By combining innovative audiovisual narrative with psychological experiments,it zooms in on real human nature through discussing social hotspots from the perspectives of social psychology,cognitive psychology,and personality psychology,in order to help people find answers for their current psychological difficulties. 展开更多
关键词 China. document INNOVATIVE
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Artificial Intelligence-Powered Legal Document Processing for Medical Negligence Cases: A Critical Review
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作者 Gobind Naidu Vicknesh Krishnan 《International Journal of Intelligence Science》 2025年第1期10-55,共46页
This critical review looks at the assessment of the application of artificial intelligence in handling legal documents with specific reference to medical negligence cases with a view of identifying its transformative ... This critical review looks at the assessment of the application of artificial intelligence in handling legal documents with specific reference to medical negligence cases with a view of identifying its transformative potentialities, issues and ethical concerns. The review consolidates findings that show the impact of AI in improving the efficiency, accuracy and justice delivery in the legal profession. The studies show increased efficiency in speed of document review and enhancement of the accuracy of the reviewed documents, with time efficiency estimates of 60% reduction of time. However, the review also outlines some of the problems that continue to characterize AI, such as data quality problems, biased algorithms and the problem of the opaque decision-making system. This paper assesses ethical issues related to patient autonomy, justice and non-malignant suffering, with particular focus on patient privacy and fair process, and on potential unfairness to patients. This paper’s review of AI innovations finds that regulations lag behind AI developments, leading to unsettled issues regarding legal responsibility for AI and user control over AI-generated results and findings in legal proceedings. Some of the future avenues that are presented in the study are the future of XAI for legal purposes, utilizing federated learning for resolving privacy issues, and the need to foster adaptive regulation. Finally, the review advocates for Legal Subject Matter Experts to collaborate with legal informatics experts, ethicists, and policy makers to develop the best solutions to implement AI in medical negligence claims. It reasons that there is great potential for AI to have a deep impact on the practice of law but when done, it must do so in a way that respects justice and on the Rights of Individuals. 展开更多
关键词 Artificial Intelligence Medical Negligence Legal document Processing Ethical Implications Regulatory Frameworks
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Chinese Documentaries
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《China Today》 2025年第3期65-65,共1页
What Are You Up To Today?Chief Director:Wu Zijuan Length:12 Episodes Producer:bilibili Broadcasting Platform:bilibili Produced by China’s YouTube-like video sharing platform bilibili,the film is a series of short doc... What Are You Up To Today?Chief Director:Wu Zijuan Length:12 Episodes Producer:bilibili Broadcasting Platform:bilibili Produced by China’s YouTube-like video sharing platform bilibili,the film is a series of short documentaries presenting people's daily life in different jobs.It follows 12 individuals in their respective jobs and trades that keep society functioning.By focusing on their daily lives,the documentary films capture the hustle and bustle of the days that make up a hopeful life. 展开更多
关键词 DIRECTOR PRODUCER document
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The Critical Role and Practice of Medical Device Design and Development Documentation in Quality Systems
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作者 Meiting He 《Journal of Clinical and Nursing Research》 2025年第8期108-115,共8页
This paper highlights the critical role of medical device design and development documents within the quality system,including their compliance with regulatory standards,their function as a traceable record,their supp... This paper highlights the critical role of medical device design and development documents within the quality system,including their compliance with regulatory standards,their function as a traceable record,their support for all stages,and their use in risk and change management.It also covers document template creation,review record association,information management,adverse event traceability,and the reconciliation of differences in international declarations. 展开更多
关键词 Medical devices Design and development documents Quality system
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IEC releases 3 technical documents for electrical energy storage
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《China Standardization》 2025年第4期14-14,共1页
In the process of building a new power system dominated by new energy sources,power storage is a key supporting technology that ensures the safe and stable operation of the power grid,enables the flexible regulation o... In the process of building a new power system dominated by new energy sources,power storage is a key supporting technology that ensures the safe and stable operation of the power grid,enables the flexible regulation of the system,and raises the level of new energy consumption.It is also key to achieving carbon peak and neutrality as well as energy transformation. 展开更多
关键词 carbon peak neutrality new energy sourcespower storage raises level new energy consumptionit technical documents power gridenables flexible regulation systemand electrical energy storage building new power system
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分子鉴别诊断(MDD)技术在呼吸道病原体检测中的应用 被引量:10
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作者 韩卫宁 张正姬 +5 位作者 雅雪蓉 季伟 衡伟 夏瑜 许元根 史智扬 《细胞与分子免疫学杂志》 CAS CSCD 北大核心 2010年第11期1146-1148,1151,共4页
目的:以靶序列富集多重PCR(Tem-PCR)和液相芯片(xMAP)检测技术结合,建立常见呼吸道病原体的分子鉴别诊断(MDD)平台,对其可靠性进行评估,并用于临床样品的检测。方法:采集苏州大学附属儿童医院呼吸科住院病例呼吸道灌洗液样品22例和苏州... 目的:以靶序列富集多重PCR(Tem-PCR)和液相芯片(xMAP)检测技术结合,建立常见呼吸道病原体的分子鉴别诊断(MDD)平台,对其可靠性进行评估,并用于临床样品的检测。方法:采集苏州大学附属儿童医院呼吸科住院病例呼吸道灌洗液样品22例和苏州大学附属第一医院呼吸内科门诊病例咽拭子样品20例,使用Tem-PCR技术对样品的核酸进行多重扩增,以液相芯片技术进行高通量的多重检测。结果:对已知样品的检测表明该平台具有高度的特异性和敏感性。对42例临床样品进行检测的结果显示,22例呼吸道灌洗液的阳性检出率为63.6%。呼吸道灌洗液阳性检出率高于门诊病例咽拭子样品的阳性检出率,RNA病原体(以病毒为主)的阳性率高于DNA病原体(以细菌为主)的阳性率。结论:建立了呼吸道病原体的MDD平台,具备了对呼吸道传染性疾病的高通量快速诊断能力。 展开更多
关键词 tem-PCR xMAP 分子鉴别诊断(mdd)
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MDD法和裂变径迹法相结合模拟样品的低温热历史--以柴达木盆地北缘赛什腾山中新生代构造演化为例 被引量:14
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作者 万景林 郑德文 +1 位作者 郑文俊 王伟涛 《地震地质》 EI CSCD 北大核心 2011年第2期369-382,共14页
MDD法和裂变径迹法是2种最常用的低温热年代学方法。通过对它们的测试结果的模拟,揭示出样品经历的热历史,可以有效地延伸这2种方法的研究范围。但是由于模拟的多解性,使得如何制约模拟过程,获得合理的模拟结果显得尤为重要。通过柴达... MDD法和裂变径迹法是2种最常用的低温热年代学方法。通过对它们的测试结果的模拟,揭示出样品经历的热历史,可以有效地延伸这2种方法的研究范围。但是由于模拟的多解性,使得如何制约模拟过程,获得合理的模拟结果显得尤为重要。通过柴达木盆地北缘的模拟过程,展示了模拟中可能存在的问题及提高模拟结果的方法。同时揭示了柴达木盆地北缘中新生代可能存在的3次冷却事件,分别发生在距今约130~150、30~40和5~10Ma。 展开更多
关键词 新生代构造 钾长石mdd 裂变径迹 快速冷却 青藏高原东北缘
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青藏高原北缘中生代伸展构造^(40)Ar/^(39)Ar测年和MDD模拟 被引量:30
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作者 陈宣华 尹安 +5 位作者 George E.Gehrels 王小凤 Eric S.Cowgill Marty Grove T.Mark Harrison 陈正乐 《地球学报》 EI CAS CSCD 北大核心 2002年第4期305-310,共6页
沿着青藏高原北缘的阿尔金山脉东段 ,发育了长度大于 30 0km、EW走向的拉配泉断裂。中美合作阿尔金课题组的地质填图结果表明 ,该断层实际上是一条南倾的正断层 ,局部倾角可以低至 30°以下。沉积学和热年代学研究控制了拉配泉断裂... 沿着青藏高原北缘的阿尔金山脉东段 ,发育了长度大于 30 0km、EW走向的拉配泉断裂。中美合作阿尔金课题组的地质填图结果表明 ,该断层实际上是一条南倾的正断层 ,局部倾角可以低至 30°以下。沉积学和热年代学研究控制了拉配泉断裂的活动时代 :早—中侏罗统地层可以解释为断裂上盘的伸展盆地沉积 ;下盘岩石中钾长石40 Ar/ 3 9Ar测年和MDD模拟给出 2个阶段的冷却事件 ,早期事件出现在约 2 2 0~ 187Ma之间 ,晚期事件出现在早白垩世晚期 (约 10 0Ma)。早期事件代表了拉配泉断裂正断作用的主要阶段。 展开更多
关键词 青藏高原 阿尔金山 拉配泉断裂 ^40Ar/^39Ar测年 mdd模拟 氩同位素 中生代
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构造非平稳运动定量研究的MDD模式制约 被引量:4
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作者 陈文寄 李齐 马宗晋 《地球科学(中国地质大学学报)》 EI CAS CSCD 北大核心 1996年第3期277-279,共3页
利用MDD模式得到的连续冷却曲线,可以定量地描述地质体的冷却(隆升)过程;采用我国7个不同地区中38个钾长石MDD模式研究结果证明,快速冷却事件存在的普遍性,不同的快速冷却速度可能反映了不同的构造事件;突变因子(δv... 利用MDD模式得到的连续冷却曲线,可以定量地描述地质体的冷却(隆升)过程;采用我国7个不同地区中38个钾长石MDD模式研究结果证明,快速冷却事件存在的普遍性,不同的快速冷却速度可能反映了不同的构造事件;突变因子(δvi)可以定量地描述地质体非平稳运动以及突变程度。 展开更多
关键词 mdd模式 突变因子 构造运动 定量研究 地质构造
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MDD模式与快速冷却事件 被引量:11
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作者 陈文寄 李齐 《地学前缘》 EI CAS CSCD 1999年第4期371-377,共7页
MDD 模式(Multiple Diffusion Domain model) 是Dodson 模式的扩展,它可以从一个钾长石样品分析中,得到温度在≤350 ~150 ℃之间的一条连续的冷却曲线,因此,MDD 模式的一个突出... MDD 模式(Multiple Diffusion Domain model) 是Dodson 模式的扩展,它可以从一个钾长石样品分析中,得到温度在≤350 ~150 ℃之间的一条连续的冷却曲线,因此,MDD 模式的一个突出贡献是对快速冷却事件的定量制约。冈底斯岩带与哀牢山- 红河剪切带中新世的快速冷却事件与构造含义是利用这一模式进行研究的成功实例,它不仅定量厘定了快速冷却事件的时间,证实了其存在的普遍性,而且明确指出了中新世印度和欧亚板块碰撞后岩石圈质量转移过程的时间序列,定量地指出了冈底斯岩带逆冲抬升导致的地壳增厚与沿哀牢山- 展开更多
关键词 mdd模式 快速冷却事件 冈底斯岩带 剪切带
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MDD模式应用中若干问题的讨论Ⅱ——活化能选取的重要性 被引量:7
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作者 李齐 陈文寄 《地震地质》 EI CSCD 北大核心 1999年第1期48-50,共3页
活化能取值在MDD模式中对冷却曲线的温度有较大的影响,其影响度大约为7~8℃/kcal·mol-1,但它并不改变冷却曲线的形状。导致活化能取值偏低的影响因素包括低温阶段的加热时间、K-长石的纯度和部分斜长石、石英... 活化能取值在MDD模式中对冷却曲线的温度有较大的影响,其影响度大约为7~8℃/kcal·mol-1,但它并不改变冷却曲线的形状。导致活化能取值偏低的影响因素包括低温阶段的加热时间、K-长石的纯度和部分斜长石、石英的混入等。 展开更多
关键词 热历史 地质年代测定 活化能 mdd模式 长石
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Documentation Concordance,Sharing and Utilization of Tea Germplasm Resources in Yunnan 被引量:3
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作者 刘本英 宋维希 +6 位作者 孙雪梅 蒋会兵 马玲 矣兵 季鹏章 汪云刚 王平盛 《Agricultural Science & Technology》 CAS 2011年第12期1842-1848,共7页
In this paper,the research achievements and progress of Yunnan tea germplasm resource in past sixty years are systematically reviewed from the following aspects:exploration,collecting,conservation,protection,identifi... In this paper,the research achievements and progress of Yunnan tea germplasm resource in past sixty years are systematically reviewed from the following aspects:exploration,collecting,conservation,protection,identification,evaluation and shared utilization.Simultaneously,the current problems and the suggestions about subsequent development of tea germplasm resources in Yunnan were discussed,including superior and rare germplasm collection,tea genetic diversity research,biotechnology utilization in tea germplasm innovation,super gene exploration and function,the construction of utilization platform,biological base of species and population conservation. 展开更多
关键词 YUNNAN Tea germplasm resource documentation Concordance SHARING UTILIZATION
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大规模图数据的k^2-MDD表示方法与操作研究 被引量:4
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作者 董荣胜 张新凯 +1 位作者 刘华东 古天龙 《计算机研究与发展》 EI CSCD 北大核心 2016年第12期2783-2792,共10页
对包含亿万个顶点和边的图数据进行高效、紧凑的表示和操作是大规模图数据分析处理的基础.针对该问题提出了基于决策图的大规模图数据的一种表示方法——k^2-MDD,给出了k^2-MDD的构造过程以及图的边查询、外(内)邻查询、出(入)度查询、... 对包含亿万个顶点和边的图数据进行高效、紧凑的表示和操作是大规模图数据分析处理的基础.针对该问题提出了基于决策图的大规模图数据的一种表示方法——k^2-MDD,给出了k^2-MDD的构造过程以及图的边查询、外(内)邻查询、出(入)度查询、添加(删除)边等基本操作.该表示方法在k^2树的基础上进行优化与改进,对图的邻接矩阵进行k^2划分后,采用多值决策图进行存储,从而达到存储结构更为紧凑的目的.通过对来自米兰大学LAW实验室的一系列真实网页图和社交网络图数据的实验结果可以看出,k^2-MDD结构在节点数上仅为k^2树的2.59%~4.51%,达到了预期效果.通过对随机图的实验结果可以看出,k^2-MDD结构不仅适用于稀疏图,同样也适用于稠密图.图数据的k^2-MDD表示,既具有k^2树表示的紧凑型和查询的高效性,又能实现符号决策图表示下图模式的高效操作,从而实现了描述和计算能力的统一. 展开更多
关键词 图数据 存储优化 々2_ M D D 々2 决策图
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冈底斯岩带热演化史的MDD模式新证据Ⅰ 被引量:2
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作者 陈文寄 郝杰 《地球学报(中国地质科学院院报)》 CSCD 北大核心 1997年第A00期86-88,共3页
本文首次报道在我国实验室利用特定的^40Ar/^39Ar阶段加热程序和MDD模式处理得到的钾长石冷却曲线,加之黑云线和磷灰石的年龄测定结果,进一步讨论了冈底斯岩带的热演化史。
关键词 西藏 热演化史 mdd模式 花岗岩 磷灰石
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Document classification approach by rough-set-based corner classification neural network 被引量:1
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作者 张卫丰 徐宝文 +1 位作者 崔自峰 徐峻岭 《Journal of Southeast University(English Edition)》 EI CAS 2006年第3期439-444,共6页
A rough set based corner classification neural network, the Rough-CC4, is presented to solve document classification problems such as document representation of different document sizes, document feature selection and... A rough set based corner classification neural network, the Rough-CC4, is presented to solve document classification problems such as document representation of different document sizes, document feature selection and document feature encoding. In the Rough-CC4, the documents are described by the equivalent classes of the approximate words. By this method, the dimensions representing the documents can be reduced, which can solve the precision problems caused by the different document sizes and also blur the differences caused by the approximate words. In the Rough-CC4, a binary encoding method is introduced, through which the importance of documents relative to each equivalent class is encoded. By this encoding method, the precision of the Rough-CC4 is improved greatly and the space complexity of the Rough-CC4 is reduced. The Rough-CC4 can be used in automatic classification of documents. 展开更多
关键词 document classification neural network rough set meta search engine
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论学界对“record”与“document”翻译之争 被引量:1
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作者 周莉莉 张伟斌 《办公室业务》 2017年第10期114-115,共2页
随着我国档案学界的发展,我们越来越注重国内外档案学界的融合。故本文通过对学界"record"和"document"观点的梳理,以期加强我国与国外档案学界的融合,从而促使我国档案事业的进一步发展。
关键词 RECORD document 文件 文档
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DYNAMIC ENGINEERING DOCUMENT MANAGEMENT BASED ON XML TECHNOLOGY
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作者 翟建军 陈文亮 丁秋林 《Transactions of Nanjing University of Aeronautics and Astronautics》 EI 2005年第1期38-41,共4页
The eXtensible markup language (XML) is a kind of new meta language for replacing HTML and has many advantages. Traditional engineering documents have too many expression forms to be expediently managed and have no dy... The eXtensible markup language (XML) is a kind of new meta language for replacing HTML and has many advantages. Traditional engineering documents have too many expression forms to be expediently managed and have no dynamic correlation functions. This paper introduces a new method and uses XML to store and manage engineering documents to realize the format unity of engineering documents and their dynamic correlations. 展开更多
关键词 XML dynamic engineering document MANAGEMENT
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