[ Objeetlve] This study aimed to investigate the role of dihydrolipoamide dehydrogenase （DLDH） in the pathopoiesis of Vibrio alginolyticua. [ Method] Using suicide plasmid pRE112 as the vector, d/dh deletion mutant ...[ Objeetlve] This study aimed to investigate the role of dihydrolipoamide dehydrogenase （DLDH） in the pathopoiesis of Vibrio alginolyticua. [ Method] Using suicide plasmid pRE112 as the vector, d/dh deletion mutant strain ZJO3Adldh was constructed with insertion inactivation mutation method by Overlap PCR and homologous recombination technology to compare and analyze the differences in the growth, swimming ability, enzyme activity, biofilm formation and pathogenicity between wild-type strain ZJ03 and deletion mutant strain ZJO3△dldh. [ Result] dldh deletion mutant strain ZJ03 △d/dh exhibited prolonged lag phase, significantly reduced swimming ability （P 〈 0.05 ）, significantly reduced enzyme activity （P 〈 0.05 ）, significantly reduced biofilm formation ability （P 〈 0.05 ） and remarkably reduced pathogenicity to Epinephelus coioides （P 〈0.05） compared to wild-type strain ZJ03. [ Conclusion] This study provided theoretical basis for revealing the pathogenic mechanisms of Vibrio from the perspective of bacterial energy metabolism.展开更多
基金Supported by National Natural Science Foundation of China(No.31402344)Natural Science Foundation of Guangdong Province(S2013040014562)
文摘[ Objeetlve] This study aimed to investigate the role of dihydrolipoamide dehydrogenase （DLDH） in the pathopoiesis of Vibrio alginolyticua. [ Method] Using suicide plasmid pRE112 as the vector, d/dh deletion mutant strain ZJO3Adldh was constructed with insertion inactivation mutation method by Overlap PCR and homologous recombination technology to compare and analyze the differences in the growth, swimming ability, enzyme activity, biofilm formation and pathogenicity between wild-type strain ZJ03 and deletion mutant strain ZJO3△dldh. [ Result] dldh deletion mutant strain ZJ03 △d/dh exhibited prolonged lag phase, significantly reduced swimming ability （P 〈 0.05 ）, significantly reduced enzyme activity （P 〈 0.05 ）, significantly reduced biofilm formation ability （P 〈 0.05 ） and remarkably reduced pathogenicity to Epinephelus coioides （P 〈0.05） compared to wild-type strain ZJ03. [ Conclusion] This study provided theoretical basis for revealing the pathogenic mechanisms of Vibrio from the perspective of bacterial energy metabolism.
