Plants serve as rich repositories of diverse chemical compounds collectively referred to as specialized metabolites.These compounds are of importance for adaptive processes,including interactions with various microbes...Plants serve as rich repositories of diverse chemical compounds collectively referred to as specialized metabolites.These compounds are of importance for adaptive processes,including interactions with various microbes both beneficial and harmful.Considering microbes as bioreactors,the chemical diversity undergoes dynamic changes when root-derived specialized metabolites(RSMs)and microbes encounter each other in the rhizosphere.Recent advancements in sequencing techniques and molecular biology tools have not only accelerated the elucidation of biosynthetic pathways of RSMs but also unveiled the significance of RSMs in plant-microbe interactions.In this review,we provide a comprehensive description of the effects of RSMs on microbe assembly in the rhizosphere and the influence of corresponding microbial changes on plant health,incorporating the most up-to-date information available.Additionally,we highlight open questions that remain for a deeper understanding of and harnessing the potential of RSM-microbe interactions to enhance plant adaptation to the environment.Finally,we propose a pipeline for investigating the intricate associations between root exometabolites and the rhizomicrobiome.展开更多
[Objective] The aim was to study the genetic diversities between Xiaogan water chestnut and wild chestnut with randomly amplified polymorphic DNA （RAPD） technology. [Method] Genetic diversities of the local cultivat...[Objective] The aim was to study the genetic diversities between Xiaogan water chestnut and wild chestnut with randomly amplified polymorphic DNA （RAPD） technology. [Method] Genetic diversities of the local cultivated water chestnut,wild chestnut,Lepironia articulata and Scirpus planiculmis Fr. Schmidt were analyzed by RAPD technology. [Result] Among the screened random primers 841,842,807 and 840,the polymorphism of amplification product of 841 was evident,and the obtained bands in electrophoresis were clear and showed good repeatability. Cluster analysis result showed that the affinity of cultivated water chestnut and wild water chestnut was nearer than that between Lepironia articulata and Scirpus planiculmis. [Conclusion] The research provides theoretical basis for cultivating high-quality new varieties of water chestnut.展开更多
Based on the analysis of the hydrocarbon geochemical characteristics in the Kuqa petroleum system of the Tarim Basin, this study discusses the causes and controlling factors of the phase diversities and their differen...Based on the analysis of the hydrocarbon geochemical characteristics in the Kuqa petroleum system of the Tarim Basin, this study discusses the causes and controlling factors of the phase diversities and their differences in geochemical features. According to the characteristics and differences in oil and gas phase, the petroleum system can be divided into five categories： oil reservoir, wet gas reservoir, condensate gas-rich reservoir, condensate gas-poor reservoir and dry gas reservoir. The causes for the diversities in oil and gas phases include diversities of the sources of parent material, maturity of natural gas and the process of hydrocarbon accumulation of different hydrocarbon phases. On the whole, the Jurassic and Triassic terrestrial source rocks are the main sources for the hydrocarbon in the Kuqa Depression. The small differences in parent material may cause diversities in oil and gas amount, but the impact is small. The differences in oil and gas phase are mainly affected by maturity and the accumulation process, which closely relates with each other. Oil and gas at different thermal evolution stage can be captured in different accumulation process.展开更多
With Maolan Nature Reserve as an example, its forest landscape characteristics and diversity were studied by utilizing GIS. The results showed that the overall forest landscape in Maolan Nature Reserve was quite good,...With Maolan Nature Reserve as an example, its forest landscape characteristics and diversity were studied by utilizing GIS. The results showed that the overall forest landscape in Maolan Nature Reserve was quite good, but with the increasing natural interferences and human activities, shrub forests and non-woodland landscape types have been multiplied; among the 3 functional zones of the Nature Reserve, landscape fragmentation degree is low in core zone and buffer zone, but high in experimental zone.展开更多
Globalization increases mobilities and multiplies identities in Asia.Singapore,a city state with diverse Asian communities,amplifies this trend.In the 1990s,Singapore recruited large numbers of Western educated Chines...Globalization increases mobilities and multiplies identities in Asia.Singapore,a city state with diverse Asian communities,amplifies this trend.In the 1990s,Singapore recruited large numbers of Western educated Chinese scholars,who formed new diasporas across Asia and became catalyst for further cultural diversities.This multifaceted Asian phenomenon can be best exemplified in Chinese scholar-director Grant Shen’s contemporary productions of the traditional Chinese opera—The West Wing(2008,2016).This study discusses issues of mobilities,identities,and diversities through The West Wing,in which the performers are predominantly from Asian diasporas.The mobility of Grant Shen from China to Singapore via the US diversifies his cultural identity,at once rooted deeply in Chinese culture while openly embracing Western liberalism and Singaporean plurality.His theatre productions,boldly adopting pop music,employing trendy language,and recreating pro-feminist scenes,vividly attest to cultural diversities.Identity issues come to the fore when this well-received play in Singapore toured Shanghai,where it was welcomed by the young but frowned upon by others as subversive to Chinese tradition.This study views the multiplicity of Singapore as a fertile land for productions foretelling the plural future Asia.展开更多
The methods of DAPI staining epifluorescence microscopy and T-RFLP analysis were used to analyze the microbial abundance and diversity in surface seawater sampled from 12 stations inside and outside of the Jiaozhou Ba...The methods of DAPI staining epifluorescence microscopy and T-RFLP analysis were used to analyze the microbial abundance and diversity in surface seawater sampled from 12 stations inside and outside of the Jiaozhou Bay during a survey on 12 and 13 September 2004. The abundance of total microbes is in the range of 10^6～ 10^7 cells/cm^3, similar to those of most semi-enclosed bays in the temperate areas in the world. The highest microbial densities occur in the northeastern part of the Jiaozhou Bay, around the mouths of Loushan and Licun Rivers and the Hongdao aquacultural farming areas, suggesting that the degree and characteristics of pollutions, along with geographical and hydrological effects, may be important determinants affecting the abundance and distribution of bacteria in the Jiaozhou Bay. Bacterial communities inside and outside of the Jiaozhou Bay can be grouped into three classes based on T-RFLP patterns and cluster analyses. Stations at the water channel of the bay mouth and outside, such as D1, D3, D5, D6 and D7, are grouped together to stand for the outside bacterial community interacting with the environment outside of the Jiaozhou Bay. Stations of the innermost side of the Jiaozhou Bay, such as A3, A5, B2 and Y1, are grouped together to stand for the residential bacteria community. Stations C1, C3 and CA are grouped together and may stand for the transitional bacterial assemblage between the residential community and the outside community. However, there is no such a defined relationship for the case of cyanobacterial diversity, indicating the fact that cyanobacteria are more flexible and adaptable to all kinds of conditions.展开更多
The oceans account for 71% of the Earth’s surface and are rich in the most advantageous and characteristic resources of marine microbes. The research on diversity is the key point for exploring and exploiting marine ...The oceans account for 71% of the Earth’s surface and are rich in the most advantageous and characteristic resources of marine microbes. The research on diversity is the key point for exploring and exploiting marine microbial resources. Giving attention to species diversity and genetic diversity of marine microbes, discovering novel metabolites with multiple functions, revealing key secondary metabolic process and the main regulation mechanisms in marine microbes, and developing a new technology of biosynthesis, are the important foundation for discovering innovative drugs and developing functional products with characteristics. The present paper has reviewed the recent advances in the four research areas of marine microbial species diversity, genetic diversity, chemical diversity and metabolic diversity in China.展开更多
Free living marine nematodes were sampled from two sandy beaches in Dalian City,in December of 2015,and April,July,and October of 2016.The spatial and seasonal variations of marine nematode species diversity(based on ...Free living marine nematodes were sampled from two sandy beaches in Dalian City,in December of 2015,and April,July,and October of 2016.The spatial and seasonal variations of marine nematode species diversity(based on species abundance dataset)and functional diversity(based on functional traits dataset:feeding types and life history strategies)were investigated to understand the environmental drivers and how they respond to specific environmental variations.Sediment granularity was revealed to be the main environmental factor causing spatial differences in nematode diversity indices between the two beaches.Species diversity indices,namely species number,Margalef index,Shannon-Wiener diversity index(H’),were higher in fine-grained sediments,while the functional diversity indices,including functional evenness,functional dispersion,and Rao’s quadratic entropy index(RaoQ),were higher in coarse-grained sediments.Nematode species diversity indices also fluctuated with seasonal variations of temperature,dissolved oxygen,pH,salinity,and sediment chlorophyll-a within the study beaches.However,functional diversity indices did not show significant seasonal variations and exhibited weak correlation with the studied environmental variables.Overall,the functional diversity indices were negatively correlated with the species diversity indices,suggesting an inconsistent response to environmental changes.A decrease in nematode species diversity in coarse sands,accompanied by an increase in functional diversity,can be regarded as an early warning signal of environment disturbance.If more biological traits are involved in calculating functional diversity indices,it will be helpful for the future study of the internal connections of species diversity and functional diversity.展开更多
The presence and introduction of new potato cultivars in Kosovo has increased the yield per unit area but reduced the cultivation of native potato population. Indigenous potato is linked to specific adaptations to loc...The presence and introduction of new potato cultivars in Kosovo has increased the yield per unit area but reduced the cultivation of native potato population. Indigenous potato is linked to specific adaptations to local fanning system and specific agro ecological conditions. This research was conducted in Prishtina region which is located in the Kosovo agro ecological region （42°38′97″ latitude and 21°08′45＂ longitude at 574 m.a.s.l ）. Plant material used in this research was 18 potato accessions with different origins. The experimental design was a randomized complete block （RCB） with three replicates, while the plot sizes were 4.20 m^2. All observation and analysis were carried out on 12 plants from each accession. All characters were measured in the field and laboratory at the normal harvest time. Morphological diversity was characterized using standard descriptors. The genetic diversity studies of the investigated potato accessions showed significant genetic variation on 17 physiological and chemical parameters, which could be largely due to that they were of different origins. The genetic diversity of potato cultivars offers a wealth of natural resources from which breeders can select the most desirable genotypes based on their favorable traits.展开更多
Synthetic chemicals have been widely used in modern agriculture as fertilizer,plant growth regulators and pesticides.Although the chemical inputs improved the crop production,it is well known about their negative effe...Synthetic chemicals have been widely used in modern agriculture as fertilizer,plant growth regulators and pesticides.Although the chemical inputs improved the crop production,it is well known about their negative effects,such as environmental residues in water,soil and animals,pest resistance and resurgence and the residues in agricultural produces.The demands for safer food and more friendly environment are now more preferred by humankind in the world.Microbes have already become an alternate for chemical inputs in some aspects in agriculture.The microbes can produce very diverse volatile organic compounds(mVOCs)which play important roles in the interactions between plant and microbes,or among intraspecies or interspecies of microbes.Many mVOCs showed diverse agroactivities,such as insecticidal,bactericidal,fungicidal,herbicidal,plant growth promotion and abiotic stress-tolerance inducing activities.The agroactive mVOCs have diverse structure-types,such as alkane,alkenes,alcohols,aldehydes,ketones,carboxylic acid,esters and lactones,ethers,aromatic ring,terpenes,heterocycles,sulphur-containing VOCs.The promising agroactive properties make it possible to apply mVOCs and their producing microorganisms or synthetic mimics or agroactive mVOCs in crop protection,and so this review focuses on the chemical diversity,agroactivities,and potential application of mVOCs.展开更多
Climate and land-use changes are predicted to impact biodiversity,threatening ecosystem services and functions.However,their combined effects on the functional diversity of mammals at the regional scale remain unclear...Climate and land-use changes are predicted to impact biodiversity,threatening ecosystem services and functions.However,their combined effects on the functional diversity of mammals at the regional scale remain unclear,especially at the beta level.Here,we use projected climate and land-use changes in China to investigate their potential effects on the alpha and beta functional diversities of terrestrial mammals under low-and high-emission scenarios.In the current projection,we showed strong positive spatial correlations between functional richness and species richness.Functional evenness(FEve),functional specialization(FSpe),and functional originality(FOri)decreased with species richness,and functional divergence(FDiv)increased first and then plateaued.Functional beta diversity was dominated by its nestedness component,in contrast to the taxonomic facet.Potential changes in species richness are more strongly influenced by land-use change under the low-emission scenario,while under the high-emission scenario,they are more strongly influenced by climate change.Changes in functional richness(FRic)were inconsistent with those in species richness,with a magnitude of decreases greater than predicted from species richness.Moreover,mammal assemblages showed potential functional differentiation(FD)across the country,and the trends exceeded those towards taxonomic differentiation(TD).Our findings help us understand the processes underlying biodiversity responses to global changes on multiple facets and provide new insight for conservation plans.展开更多
Helicobacter pylori(H.pylori)infection plays a critical role in gastric diseases,impacting the microbiota structure in gastric and duodenal ulcers.In their study,Jin et al utilized metagenomic sequencing to analyze mu...Helicobacter pylori(H.pylori)infection plays a critical role in gastric diseases,impacting the microbiota structure in gastric and duodenal ulcers.In their study,Jin et al utilized metagenomic sequencing to analyze mucosal samples from patients with ulcers and healthy controls,revealing significant changes in microbial diversity and composition.This article reviews their findings,emphasizing H.pylori’s role in gastric ulcers and the need for further research on its impact on duodenal ulcers.We evaluate the study’s strengths and limitations,suggesting future research directions to enhance our understanding of H.pylori’s contribution to ulcerative diseases.展开更多
Paeonia suffruticosa Andr.is an endemic shrub flower in China with 2n=10.This study used 228 cultivars from four populations,i.e.,Jiangnan,Japan,Northwest,and Zhongyuan,as materials to explore the genetic diversity le...Paeonia suffruticosa Andr.is an endemic shrub flower in China with 2n=10.This study used 228 cultivars from four populations,i.e.,Jiangnan,Japan,Northwest,and Zhongyuan,as materials to explore the genetic diversity levels among different populations of tree peony varieties.The results showed that 34 bands were amplified using five pairs of cp SSR primers,with an average of 6.8 bands per primer pair.The average number of different alleles(N_(a)),effective alleles(N_(e)),Shannon's information index(I),diversity(H),and polymorphic information content(PIC)were 3.600,2.053,0.708,0.433,and 0.388,respectively.The PIC value was between 0.250 and 0.500,indicating a moderate level of polymorphism for the five cp SSR primer pairs.The genetic diversity levels of peony cultivars varied among different populations,with the Northwest population showing relatively lower levels(I=0.590,H=0.289,and PIC=0.263).A total of 52 haplotypes were identified in the four examined populations,and the number of haplotypes per population ranged from 11 to 22.Forty-four private haplotypes were detected across populations,and the Northwest population exhibiting the highest count of private haplotypes with 17.The mean number of effective number of haplotypes(N_(eh)),haplotypic richness(R_(h)),and diversity(H)were 8.351,6.824,and 0.893,respectively.Analysis of molecular variance indicated that genetic variation within tree peony germplasm was greater than that between germplasm resources,and the main variation was found within individuals of peony germplasm.Cluster analysis,principal coordinate analysis,and genetic structure analysis classified tree peonies from different origins into two groups,indicating a certain degree of genetic differentiation among these four tree peony cultivation groups.This study provides a theoretical basis for the exploration,utilization,and conservation of peony germplasm resources,as well as for research on the breeding of excellent varieties.展开更多
Addressing the global challenge of uranium(U)-contaminated groundwater requires innovative bioremediation strategies.This study investigates Desulfovibrio desulfuricans,a neutrophilic and mesophilic sulfate-reducing b...Addressing the global challenge of uranium(U)-contaminated groundwater requires innovative bioremediation strategies.This study investigates Desulfovibrio desulfuricans,a neutrophilic and mesophilic sulfate-reducing bacteria(SRB)strain optimized for lowtemperature(15℃)and acidic(initial pH 4)conditions,to validate its bioaugmentation potential for uranium decontamination in groundwater.Our research aimed to assess its efficacy in treating U-contaminated groundwater and elucidate the optimal growth conditions for this strain in acidic and sulfate-enriched environments.We found that D.desulfuricans was phylogenetically distinct from the native microbial community in acidic Ucontaminated groundwater,while it maintained appreciable activity in sulfate reduction under contaminated groundwater conditions after accumulation.Acid-tolerant D.desulfuricans removed 75.87%of uranium and 30.64%of sulfate from acidic U-contaminated groundwater(pH 4.0)at 15℃ within 14 days.Furthermore,we explored the optimal sulfate concentration for bacterial growth,which was found to be 2000 mg/L,and an elevated Fe^(2+) concentration from 100 to 1000 mg/L increasingly stimulated sulfate-reducing activity.These findings provide a novel insight into the application of neutrophilic and mesophilic SRB in bioremediation of acidic and low-temperature groundwater after accumulation and underscore the feasibility of bioremediation by using exogenously pure SRB.展开更多
The burgeoning market for lithium-ion batteries has stimulated a growing need for more reliable battery performance monitoring. Accurate state-of-health(SOH) estimation is critical for ensuring battery operational per...The burgeoning market for lithium-ion batteries has stimulated a growing need for more reliable battery performance monitoring. Accurate state-of-health(SOH) estimation is critical for ensuring battery operational performance. Despite numerous data-driven methods reported in existing research for battery SOH estimation, these methods often exhibit inconsistent performance across different application scenarios. To address this issue and overcome the performance limitations of individual data-driven models,integrating multiple models for SOH estimation has received considerable attention. Ensemble learning(EL) typically leverages the strengths of multiple base models to achieve more robust and accurate outputs. However, the lack of a clear review of current research hinders the further development of ensemble methods in SOH estimation. Therefore, this paper comprehensively reviews multi-model ensemble learning methods for battery SOH estimation. First, existing ensemble methods are systematically categorized into 6 classes based on their combination strategies. Different realizations and underlying connections are meticulously analyzed for each category of EL methods, highlighting distinctions, innovations, and typical applications. Subsequently, these ensemble methods are comprehensively compared in terms of base models, combination strategies, and publication trends. Evaluations across 6 dimensions underscore the outstanding performance of stacking-based ensemble methods. Following this, these ensemble methods are further inspected from the perspectives of weighted ensemble and diversity, aiming to inspire potential approaches for enhancing ensemble performance. Moreover, addressing challenges such as base model selection, measuring model robustness and uncertainty, and interpretability of ensemble models in practical applications is emphasized. Finally, future research prospects are outlined, specifically noting that deep learning ensemble is poised to advance ensemble methods for battery SOH estimation. The convergence of advanced machine learning with ensemble learning is anticipated to yield valuable avenues for research. Accelerated research in ensemble learning holds promising prospects for achieving more accurate and reliable battery SOH estimation under real-world conditions.展开更多
Wetland degradation is an escalating global challenge with profound impacts on animal diversity,particularly during successional processes.Birds,as highly mobile and environmentally sensitive organisms,serve as effect...Wetland degradation is an escalating global challenge with profound impacts on animal diversity,particularly during successional processes.Birds,as highly mobile and environmentally sensitive organisms,serve as effective indicators of ecological change.While previous studies have primarily focused on local community structures and species diversity during a specific season,there is a need to extend the research timeframe and explore broader spatial variations.Additionally,expanding from simple species diversity indices to more multidimensional diversity indices would provide a more comprehensive understanding of wetland health and resilience.To address these gaps,we investigated the effects of wetland degradation on bird diversity across taxonomic,phylogenetic,and functional dimensions in the Zoige Wetland,a plateau meadow wetland biodiversity hotspot.Surveys were conducted during both breeding(summer)and overwintering(winter)seasons across 20 transects in 5 sampling areas,representing 4 degradation levels(pristine,low,medium,and high).Our study recorded a total of 106 bird species from 32 families and 14 orders,revealing distinct seasonal patterns in bird community composition and diversity.Biodiversity indices were significantly higher in pristine and low-degraded wetlands,particularly benefiting waterfowl(Anseriformes,Ciconiiformes)and wading birds(Charadriiformes)in winter,when these areas provided superior food resources and habitat conditions.In contrast,medium and highly degraded wetlands supported increased numbers of terrestrial birds(Passeriformes)and raptors(Accipitriformes,Falconiformes).Seasonal differences in taxonomic,phylogenetic,and functional diversity indices highlighted the contrasting ecological roles of wetlands during breeding and overwintering periods.Furthermore,indicator species analysis revealed key species associated with specific degradation levels and seasons,providing valuable insights into wetland health.This study underscores the importance of spatiotemporal dynamics in understanding avian responses to wetland degradation.By linking seasonal patterns of bird diversity to habitat conditions,our findings contribute to conservation efforts and provide a framework for assessing wetland degradation and its ecological impacts.展开更多
Marine fish diversity in the Beibu Gulf from 2014 to 2022 was analyzed,using various methods including at-sea fisheries resource surveys,fishing port surveys,underwater survey techniques,and data from literature,books...Marine fish diversity in the Beibu Gulf from 2014 to 2022 was analyzed,using various methods including at-sea fisheries resource surveys,fishing port surveys,underwater survey techniques,and data from literature,books,and databases,from which 1059 fish species across 40 orders,166 families,and 503 genera were identified.Among them,Acanthuriformes displayed the highest diversity,followed by Carangiformes and Perciformes.Notably,eight alien species were found in the Beibu Gulf.The International Union for Conservation of Nature(IUCN)Red List assessment revealed 51threatened species,primarily cartilaginous fish.Taxonomic changes affected 88.70%of species due to classification adjustments,misidentifications,and junior synonymy.This study contributed new data of the gulf,including one additional order,six new families,34 new genera,and 81 new species,emphasizing the dynamic nature of marine ecosystems.Fish diversity in the gulf was relatively lower than the broader South China Sea,attributed to limited habitats and survey methods.Different survey methods,such as gill nets,trap nets,and underwater cameras,yielded varying results.Ocean currents may have transported deep-sea species into the Beibu Gulf,leading to accidental records.Research gaps exist in unexplored areas,warranting further investigation.Therefore,the Beibu Gulf hosts significant marine biodiversity,but taxonomic challenges and undiscovered species require new research and conservation efforts.展开更多
Viral infectious diseases,characterized by their intricate nature and wide-ranging diversity,pose substantial challenges in the domain of data management.The vast volume of data generated by these diseases,spanning fr...Viral infectious diseases,characterized by their intricate nature and wide-ranging diversity,pose substantial challenges in the domain of data management.The vast volume of data generated by these diseases,spanning from the molecular mechanisms within cells to large-scale epidemiological patterns,has surpassed the capabilities of traditional analytical methods.In the era of artificial intelligence(AI)and big data,there is an urgent necessity for the optimization of these analytical methods to more effectively handle and utilize the information.Despite the rapid accumulation of data associated with viral infections,the lack of a comprehensive framework for integrating,selecting,and analyzing these datasets has left numerous researchers uncertain about which data to select,how to access it,and how to utilize it most effectively in their research.This review endeavors to fill these gaps by exploring the multifaceted nature of viral infectious diseases and summarizing relevant data across multiple levels,from the molecular details of pathogens to broad epidemiological trends.The scope extends from the micro-scale to the macro-scale,encompassing pathogens,hosts,and vectors.In addition to data summarization,this review thoroughly investigates various dataset sources.It also traces the historical evolution of data collection in the field of viral infectious diseases,highlighting the progress achieved over time.Simultaneously,it evaluates the current limitations that impede data utilization.Furthermore,we propose strategies to surmount these challenges,focusing on the development and application of advanced computational techniques,AI-driven models,and enhanced data integration practices.By providing a comprehensive synthesis of existing knowledge,this review is designed to guide future research and contribute to more informed approaches in the surveillance,prevention,and control of viral infectious diseases,particularly within the context of the expanding big-data landscape.展开更多
基金National Key Research and Development Program of China(2018YFA0900603 to G.W.and 2022YFF1001800 to Y.B.)the National Natural Science Foundation of China(grant No.32000232)to X.W.the State Key Laboratory of Plant Genomics of China(SKLPG2016A-13)to G.W.
文摘Plants serve as rich repositories of diverse chemical compounds collectively referred to as specialized metabolites.These compounds are of importance for adaptive processes,including interactions with various microbes both beneficial and harmful.Considering microbes as bioreactors,the chemical diversity undergoes dynamic changes when root-derived specialized metabolites(RSMs)and microbes encounter each other in the rhizosphere.Recent advancements in sequencing techniques and molecular biology tools have not only accelerated the elucidation of biosynthetic pathways of RSMs but also unveiled the significance of RSMs in plant-microbe interactions.In this review,we provide a comprehensive description of the effects of RSMs on microbe assembly in the rhizosphere and the influence of corresponding microbial changes on plant health,incorporating the most up-to-date information available.Additionally,we highlight open questions that remain for a deeper understanding of and harnessing the potential of RSM-microbe interactions to enhance plant adaptation to the environment.Finally,we propose a pipeline for investigating the intricate associations between root exometabolites and the rhizomicrobiome.
基金Supported by Natural Science Foundation of Hubei Province(2005ABA084)Major Projects of Hubei Provincial Department of Education (04Z002)~~
文摘[Objective] The aim was to study the genetic diversities between Xiaogan water chestnut and wild chestnut with randomly amplified polymorphic DNA （RAPD） technology. [Method] Genetic diversities of the local cultivated water chestnut,wild chestnut,Lepironia articulata and Scirpus planiculmis Fr. Schmidt were analyzed by RAPD technology. [Result] Among the screened random primers 841,842,807 and 840,the polymorphism of amplification product of 841 was evident,and the obtained bands in electrophoresis were clear and showed good repeatability. Cluster analysis result showed that the affinity of cultivated water chestnut and wild water chestnut was nearer than that between Lepironia articulata and Scirpus planiculmis. [Conclusion] The research provides theoretical basis for cultivating high-quality new varieties of water chestnut.
基金supported by the Tarim Oil Field Research InstituteNational Natural Science Foundation (sanctified number:40602016)National Key Basic Research and Development Projects (Itemnumber:113404GJ0003)
文摘Based on the analysis of the hydrocarbon geochemical characteristics in the Kuqa petroleum system of the Tarim Basin, this study discusses the causes and controlling factors of the phase diversities and their differences in geochemical features. According to the characteristics and differences in oil and gas phase, the petroleum system can be divided into five categories： oil reservoir, wet gas reservoir, condensate gas-rich reservoir, condensate gas-poor reservoir and dry gas reservoir. The causes for the diversities in oil and gas phases include diversities of the sources of parent material, maturity of natural gas and the process of hydrocarbon accumulation of different hydrocarbon phases. On the whole, the Jurassic and Triassic terrestrial source rocks are the main sources for the hydrocarbon in the Kuqa Depression. The small differences in parent material may cause diversities in oil and gas amount, but the impact is small. The differences in oil and gas phase are mainly affected by maturity and the accumulation process, which closely relates with each other. Oil and gas at different thermal evolution stage can be captured in different accumulation process.
基金Supported by National Natural Science Foundation(40971160)Project of Guizhou Provincial Natural Science Foundation(2007-2049)+2 种基金Guizhou Provincial High-grade Talents Project(TZJF-2008-40)Supporting Project for Guizhou Provincial Outstanding Young S&T Talents in 2009Key Project of Guizhou Normal University Students'Tasks~~
文摘With Maolan Nature Reserve as an example, its forest landscape characteristics and diversity were studied by utilizing GIS. The results showed that the overall forest landscape in Maolan Nature Reserve was quite good, but with the increasing natural interferences and human activities, shrub forests and non-woodland landscape types have been multiplied; among the 3 functional zones of the Nature Reserve, landscape fragmentation degree is low in core zone and buffer zone, but high in experimental zone.
文摘Globalization increases mobilities and multiplies identities in Asia.Singapore,a city state with diverse Asian communities,amplifies this trend.In the 1990s,Singapore recruited large numbers of Western educated Chinese scholars,who formed new diasporas across Asia and became catalyst for further cultural diversities.This multifaceted Asian phenomenon can be best exemplified in Chinese scholar-director Grant Shen’s contemporary productions of the traditional Chinese opera—The West Wing(2008,2016).This study discusses issues of mobilities,identities,and diversities through The West Wing,in which the performers are predominantly from Asian diasporas.The mobility of Grant Shen from China to Singapore via the US diversifies his cultural identity,at once rooted deeply in Chinese culture while openly embracing Western liberalism and Singaporean plurality.His theatre productions,boldly adopting pop music,employing trendy language,and recreating pro-feminist scenes,vividly attest to cultural diversities.Identity issues come to the fore when this well-received play in Singapore toured Shanghai,where it was welcomed by the young but frowned upon by others as subversive to Chinese tradition.This study views the multiplicity of Singapore as a fertile land for productions foretelling the plural future Asia.
基金financially supported by the Pilot Projects of Knowledge Innovation Project,Chinese Academy of Sciences under contract Nos KZCX3-SW-214,KZCX3-SW-223 and KZCX3-SW-233the National Natural Science Foundation of China under contract Nos 40476058 and 40576069.
文摘The methods of DAPI staining epifluorescence microscopy and T-RFLP analysis were used to analyze the microbial abundance and diversity in surface seawater sampled from 12 stations inside and outside of the Jiaozhou Bay during a survey on 12 and 13 September 2004. The abundance of total microbes is in the range of 10^6～ 10^7 cells/cm^3, similar to those of most semi-enclosed bays in the temperate areas in the world. The highest microbial densities occur in the northeastern part of the Jiaozhou Bay, around the mouths of Loushan and Licun Rivers and the Hongdao aquacultural farming areas, suggesting that the degree and characteristics of pollutions, along with geographical and hydrological effects, may be important determinants affecting the abundance and distribution of bacteria in the Jiaozhou Bay. Bacterial communities inside and outside of the Jiaozhou Bay can be grouped into three classes based on T-RFLP patterns and cluster analyses. Stations at the water channel of the bay mouth and outside, such as D1, D3, D5, D6 and D7, are grouped together to stand for the outside bacterial community interacting with the environment outside of the Jiaozhou Bay. Stations of the innermost side of the Jiaozhou Bay, such as A3, A5, B2 and Y1, are grouped together to stand for the residential bacteria community. Stations C1, C3 and CA are grouped together and may stand for the transitional bacterial assemblage between the residential community and the outside community. However, there is no such a defined relationship for the case of cyanobacterial diversity, indicating the fact that cyanobacteria are more flexible and adaptable to all kinds of conditions.
文摘The oceans account for 71% of the Earth’s surface and are rich in the most advantageous and characteristic resources of marine microbes. The research on diversity is the key point for exploring and exploiting marine microbial resources. Giving attention to species diversity and genetic diversity of marine microbes, discovering novel metabolites with multiple functions, revealing key secondary metabolic process and the main regulation mechanisms in marine microbes, and developing a new technology of biosynthesis, are the important foundation for discovering innovative drugs and developing functional products with characteristics. The present paper has reviewed the recent advances in the four research areas of marine microbial species diversity, genetic diversity, chemical diversity and metabolic diversity in China.
基金This study was supported by the National Natural Science Foundation of China(Nos.41976100,41576153).
文摘Free living marine nematodes were sampled from two sandy beaches in Dalian City,in December of 2015,and April,July,and October of 2016.The spatial and seasonal variations of marine nematode species diversity(based on species abundance dataset)and functional diversity(based on functional traits dataset:feeding types and life history strategies)were investigated to understand the environmental drivers and how they respond to specific environmental variations.Sediment granularity was revealed to be the main environmental factor causing spatial differences in nematode diversity indices between the two beaches.Species diversity indices,namely species number,Margalef index,Shannon-Wiener diversity index(H’),were higher in fine-grained sediments,while the functional diversity indices,including functional evenness,functional dispersion,and Rao’s quadratic entropy index(RaoQ),were higher in coarse-grained sediments.Nematode species diversity indices also fluctuated with seasonal variations of temperature,dissolved oxygen,pH,salinity,and sediment chlorophyll-a within the study beaches.However,functional diversity indices did not show significant seasonal variations and exhibited weak correlation with the studied environmental variables.Overall,the functional diversity indices were negatively correlated with the species diversity indices,suggesting an inconsistent response to environmental changes.A decrease in nematode species diversity in coarse sands,accompanied by an increase in functional diversity,can be regarded as an early warning signal of environment disturbance.If more biological traits are involved in calculating functional diversity indices,it will be helpful for the future study of the internal connections of species diversity and functional diversity.
文摘The presence and introduction of new potato cultivars in Kosovo has increased the yield per unit area but reduced the cultivation of native potato population. Indigenous potato is linked to specific adaptations to local fanning system and specific agro ecological conditions. This research was conducted in Prishtina region which is located in the Kosovo agro ecological region （42°38′97″ latitude and 21°08′45＂ longitude at 574 m.a.s.l ）. Plant material used in this research was 18 potato accessions with different origins. The experimental design was a randomized complete block （RCB） with three replicates, while the plot sizes were 4.20 m^2. All observation and analysis were carried out on 12 plants from each accession. All characters were measured in the field and laboratory at the normal harvest time. Morphological diversity was characterized using standard descriptors. The genetic diversity studies of the investigated potato accessions showed significant genetic variation on 17 physiological and chemical parameters, which could be largely due to that they were of different origins. The genetic diversity of potato cultivars offers a wealth of natural resources from which breeders can select the most desirable genotypes based on their favorable traits.
基金the Foundation of Hubei Hongshan Labororaty(No.2021hskf016)Hubei Agricultural Science and Technology Innovation Center(2021-620-000-001-027)for financial support for this research.
文摘Synthetic chemicals have been widely used in modern agriculture as fertilizer,plant growth regulators and pesticides.Although the chemical inputs improved the crop production,it is well known about their negative effects,such as environmental residues in water,soil and animals,pest resistance and resurgence and the residues in agricultural produces.The demands for safer food and more friendly environment are now more preferred by humankind in the world.Microbes have already become an alternate for chemical inputs in some aspects in agriculture.The microbes can produce very diverse volatile organic compounds(mVOCs)which play important roles in the interactions between plant and microbes,or among intraspecies or interspecies of microbes.Many mVOCs showed diverse agroactivities,such as insecticidal,bactericidal,fungicidal,herbicidal,plant growth promotion and abiotic stress-tolerance inducing activities.The agroactive mVOCs have diverse structure-types,such as alkane,alkenes,alcohols,aldehydes,ketones,carboxylic acid,esters and lactones,ethers,aromatic ring,terpenes,heterocycles,sulphur-containing VOCs.The promising agroactive properties make it possible to apply mVOCs and their producing microorganisms or synthetic mimics or agroactive mVOCs in crop protection,and so this review focuses on the chemical diversity,agroactivities,and potential application of mVOCs.
基金supported by the National Natural Science Foundation of China(32225033,32071496)Ministry of Science and Technology of China(2022YFF1301500)。
文摘Climate and land-use changes are predicted to impact biodiversity,threatening ecosystem services and functions.However,their combined effects on the functional diversity of mammals at the regional scale remain unclear,especially at the beta level.Here,we use projected climate and land-use changes in China to investigate their potential effects on the alpha and beta functional diversities of terrestrial mammals under low-and high-emission scenarios.In the current projection,we showed strong positive spatial correlations between functional richness and species richness.Functional evenness(FEve),functional specialization(FSpe),and functional originality(FOri)decreased with species richness,and functional divergence(FDiv)increased first and then plateaued.Functional beta diversity was dominated by its nestedness component,in contrast to the taxonomic facet.Potential changes in species richness are more strongly influenced by land-use change under the low-emission scenario,while under the high-emission scenario,they are more strongly influenced by climate change.Changes in functional richness(FRic)were inconsistent with those in species richness,with a magnitude of decreases greater than predicted from species richness.Moreover,mammal assemblages showed potential functional differentiation(FD)across the country,and the trends exceeded those towards taxonomic differentiation(TD).Our findings help us understand the processes underlying biodiversity responses to global changes on multiple facets and provide new insight for conservation plans.
文摘Helicobacter pylori(H.pylori)infection plays a critical role in gastric diseases,impacting the microbiota structure in gastric and duodenal ulcers.In their study,Jin et al utilized metagenomic sequencing to analyze mucosal samples from patients with ulcers and healthy controls,revealing significant changes in microbial diversity and composition.This article reviews their findings,emphasizing H.pylori’s role in gastric ulcers and the need for further research on its impact on duodenal ulcers.We evaluate the study’s strengths and limitations,suggesting future research directions to enhance our understanding of H.pylori’s contribution to ulcerative diseases.
基金supported by Innovation Scientists and Technicians Troop Construction Projects of Henan Province(Grant No.212101510003)the Central Plains Scholar Workstation Project(Grant No.224400510002)+1 种基金the Youth Science Foundation of Henan Province(Grant No.202300410136)the Experimental Development Foundation of Henan University of Science and Technology(Grant No.SY2324004)。
文摘Paeonia suffruticosa Andr.is an endemic shrub flower in China with 2n=10.This study used 228 cultivars from four populations,i.e.,Jiangnan,Japan,Northwest,and Zhongyuan,as materials to explore the genetic diversity levels among different populations of tree peony varieties.The results showed that 34 bands were amplified using five pairs of cp SSR primers,with an average of 6.8 bands per primer pair.The average number of different alleles(N_(a)),effective alleles(N_(e)),Shannon's information index(I),diversity(H),and polymorphic information content(PIC)were 3.600,2.053,0.708,0.433,and 0.388,respectively.The PIC value was between 0.250 and 0.500,indicating a moderate level of polymorphism for the five cp SSR primer pairs.The genetic diversity levels of peony cultivars varied among different populations,with the Northwest population showing relatively lower levels(I=0.590,H=0.289,and PIC=0.263).A total of 52 haplotypes were identified in the four examined populations,and the number of haplotypes per population ranged from 11 to 22.Forty-four private haplotypes were detected across populations,and the Northwest population exhibiting the highest count of private haplotypes with 17.The mean number of effective number of haplotypes(N_(eh)),haplotypic richness(R_(h)),and diversity(H)were 8.351,6.824,and 0.893,respectively.Analysis of molecular variance indicated that genetic variation within tree peony germplasm was greater than that between germplasm resources,and the main variation was found within individuals of peony germplasm.Cluster analysis,principal coordinate analysis,and genetic structure analysis classified tree peonies from different origins into two groups,indicating a certain degree of genetic differentiation among these four tree peony cultivation groups.This study provides a theoretical basis for the exploration,utilization,and conservation of peony germplasm resources,as well as for research on the breeding of excellent varieties.
基金supported by the Centralized R&D Project of China National Nuclear Corporation(CNNC[2021]No.144)the Key Research and Development Program of Hunan Province(Nos.2022SK2076 and 2020WK2022)+2 种基金the Natural Science Foundation of Changsha(No.kq2202089)the Postdoctoral Fellowship Program of CPSF(No.BX20230437)the Natural Science Foundation of Hunan Province(No.2023JJ30658).
文摘Addressing the global challenge of uranium(U)-contaminated groundwater requires innovative bioremediation strategies.This study investigates Desulfovibrio desulfuricans,a neutrophilic and mesophilic sulfate-reducing bacteria(SRB)strain optimized for lowtemperature(15℃)and acidic(initial pH 4)conditions,to validate its bioaugmentation potential for uranium decontamination in groundwater.Our research aimed to assess its efficacy in treating U-contaminated groundwater and elucidate the optimal growth conditions for this strain in acidic and sulfate-enriched environments.We found that D.desulfuricans was phylogenetically distinct from the native microbial community in acidic Ucontaminated groundwater,while it maintained appreciable activity in sulfate reduction under contaminated groundwater conditions after accumulation.Acid-tolerant D.desulfuricans removed 75.87%of uranium and 30.64%of sulfate from acidic U-contaminated groundwater(pH 4.0)at 15℃ within 14 days.Furthermore,we explored the optimal sulfate concentration for bacterial growth,which was found to be 2000 mg/L,and an elevated Fe^(2+) concentration from 100 to 1000 mg/L increasingly stimulated sulfate-reducing activity.These findings provide a novel insight into the application of neutrophilic and mesophilic SRB in bioremediation of acidic and low-temperature groundwater after accumulation and underscore the feasibility of bioremediation by using exogenously pure SRB.
基金National Natural Science Foundation of China (52075420)Fundamental Research Funds for the Central Universities (xzy022023049)National Key Research and Development Program of China (2023YFB3408600)。
文摘The burgeoning market for lithium-ion batteries has stimulated a growing need for more reliable battery performance monitoring. Accurate state-of-health(SOH) estimation is critical for ensuring battery operational performance. Despite numerous data-driven methods reported in existing research for battery SOH estimation, these methods often exhibit inconsistent performance across different application scenarios. To address this issue and overcome the performance limitations of individual data-driven models,integrating multiple models for SOH estimation has received considerable attention. Ensemble learning(EL) typically leverages the strengths of multiple base models to achieve more robust and accurate outputs. However, the lack of a clear review of current research hinders the further development of ensemble methods in SOH estimation. Therefore, this paper comprehensively reviews multi-model ensemble learning methods for battery SOH estimation. First, existing ensemble methods are systematically categorized into 6 classes based on their combination strategies. Different realizations and underlying connections are meticulously analyzed for each category of EL methods, highlighting distinctions, innovations, and typical applications. Subsequently, these ensemble methods are comprehensively compared in terms of base models, combination strategies, and publication trends. Evaluations across 6 dimensions underscore the outstanding performance of stacking-based ensemble methods. Following this, these ensemble methods are further inspected from the perspectives of weighted ensemble and diversity, aiming to inspire potential approaches for enhancing ensemble performance. Moreover, addressing challenges such as base model selection, measuring model robustness and uncertainty, and interpretability of ensemble models in practical applications is emphasized. Finally, future research prospects are outlined, specifically noting that deep learning ensemble is poised to advance ensemble methods for battery SOH estimation. The convergence of advanced machine learning with ensemble learning is anticipated to yield valuable avenues for research. Accelerated research in ensemble learning holds promising prospects for achieving more accurate and reliable battery SOH estimation under real-world conditions.
基金supported by the Southwest Minzu University Research Startup Funds (No.16011221038,RQD2022021)Double World-Class Project (No.CX2023010)。
文摘Wetland degradation is an escalating global challenge with profound impacts on animal diversity,particularly during successional processes.Birds,as highly mobile and environmentally sensitive organisms,serve as effective indicators of ecological change.While previous studies have primarily focused on local community structures and species diversity during a specific season,there is a need to extend the research timeframe and explore broader spatial variations.Additionally,expanding from simple species diversity indices to more multidimensional diversity indices would provide a more comprehensive understanding of wetland health and resilience.To address these gaps,we investigated the effects of wetland degradation on bird diversity across taxonomic,phylogenetic,and functional dimensions in the Zoige Wetland,a plateau meadow wetland biodiversity hotspot.Surveys were conducted during both breeding(summer)and overwintering(winter)seasons across 20 transects in 5 sampling areas,representing 4 degradation levels(pristine,low,medium,and high).Our study recorded a total of 106 bird species from 32 families and 14 orders,revealing distinct seasonal patterns in bird community composition and diversity.Biodiversity indices were significantly higher in pristine and low-degraded wetlands,particularly benefiting waterfowl(Anseriformes,Ciconiiformes)and wading birds(Charadriiformes)in winter,when these areas provided superior food resources and habitat conditions.In contrast,medium and highly degraded wetlands supported increased numbers of terrestrial birds(Passeriformes)and raptors(Accipitriformes,Falconiformes).Seasonal differences in taxonomic,phylogenetic,and functional diversity indices highlighted the contrasting ecological roles of wetlands during breeding and overwintering periods.Furthermore,indicator species analysis revealed key species associated with specific degradation levels and seasons,providing valuable insights into wetland health.This study underscores the importance of spatiotemporal dynamics in understanding avian responses to wetland degradation.By linking seasonal patterns of bird diversity to habitat conditions,our findings contribute to conservation efforts and provide a framework for assessing wetland degradation and its ecological impacts.
基金Supported by the Regional Innovation Development Joint Fund Project of the National Natural Science Foundation of China(No.U20A2087)the Shiptime Sharing Project of National Natural Science Foundation of China(No.42249911)。
文摘Marine fish diversity in the Beibu Gulf from 2014 to 2022 was analyzed,using various methods including at-sea fisheries resource surveys,fishing port surveys,underwater survey techniques,and data from literature,books,and databases,from which 1059 fish species across 40 orders,166 families,and 503 genera were identified.Among them,Acanthuriformes displayed the highest diversity,followed by Carangiformes and Perciformes.Notably,eight alien species were found in the Beibu Gulf.The International Union for Conservation of Nature(IUCN)Red List assessment revealed 51threatened species,primarily cartilaginous fish.Taxonomic changes affected 88.70%of species due to classification adjustments,misidentifications,and junior synonymy.This study contributed new data of the gulf,including one additional order,six new families,34 new genera,and 81 new species,emphasizing the dynamic nature of marine ecosystems.Fish diversity in the gulf was relatively lower than the broader South China Sea,attributed to limited habitats and survey methods.Different survey methods,such as gill nets,trap nets,and underwater cameras,yielded varying results.Ocean currents may have transported deep-sea species into the Beibu Gulf,leading to accidental records.Research gaps exist in unexplored areas,warranting further investigation.Therefore,the Beibu Gulf hosts significant marine biodiversity,but taxonomic challenges and undiscovered species require new research and conservation efforts.
基金supported by the National Natural Science Foundation of China(32370703)the CAMS Innovation Fund for Medical Sciences(CIFMS)(2022-I2M-1-021,2021-I2M-1-061)the Major Project of Guangzhou National Labora-tory(GZNL2024A01015).
文摘Viral infectious diseases,characterized by their intricate nature and wide-ranging diversity,pose substantial challenges in the domain of data management.The vast volume of data generated by these diseases,spanning from the molecular mechanisms within cells to large-scale epidemiological patterns,has surpassed the capabilities of traditional analytical methods.In the era of artificial intelligence(AI)and big data,there is an urgent necessity for the optimization of these analytical methods to more effectively handle and utilize the information.Despite the rapid accumulation of data associated with viral infections,the lack of a comprehensive framework for integrating,selecting,and analyzing these datasets has left numerous researchers uncertain about which data to select,how to access it,and how to utilize it most effectively in their research.This review endeavors to fill these gaps by exploring the multifaceted nature of viral infectious diseases and summarizing relevant data across multiple levels,from the molecular details of pathogens to broad epidemiological trends.The scope extends from the micro-scale to the macro-scale,encompassing pathogens,hosts,and vectors.In addition to data summarization,this review thoroughly investigates various dataset sources.It also traces the historical evolution of data collection in the field of viral infectious diseases,highlighting the progress achieved over time.Simultaneously,it evaluates the current limitations that impede data utilization.Furthermore,we propose strategies to surmount these challenges,focusing on the development and application of advanced computational techniques,AI-driven models,and enhanced data integration practices.By providing a comprehensive synthesis of existing knowledge,this review is designed to guide future research and contribute to more informed approaches in the surveillance,prevention,and control of viral infectious diseases,particularly within the context of the expanding big-data landscape.
