Prostate-specific membrane antigen (PSMA) is a cellsurface glycoprotein expressed predominantly in prostatesecretory acinar epithelium and prostate cancer cells aswell as in several extraprostatic tissues. Mouse monoc...Prostate-specific membrane antigen (PSMA) is a cellsurface glycoprotein expressed predominantly in prostatesecretory acinar epithelium and prostate cancer cells aswell as in several extraprostatic tissues. Mouse monoclonal antibody 4G5 specific to the extracellular domainof PSMA was used to screen two phage displayed peptide libraries (9aa linear and 9aa cys library). Three 4G5reactive phagotopes were identified. Sequence analysis ofisolated clones demonstrated that the interaction motif'VDPA/SK' has high homology to 719-725aa on PSMA.Immunohistochemical staming of the prostate cancer sam ple with the PSMA-mimic phagotope (mimotope) immunized serum antibodies demonstrate that the mimotopeisolated from the phage displayed peptide libraries can induce PSMA specific immune response in vivo.展开更多
BERNARDO Bertolucci’s film, The Last Emperor, drew its Western audience into a mystic, grandiose, oriental palace that was the Forbidden City, leaving them awestruck at the
The primers were designed according to the gene sequence of lipocalin protein family,and the gene sequence containing random mutation protein was obtained by overlapping extension of PCR.The random mutation lipocalin ...The primers were designed according to the gene sequence of lipocalin protein family,and the gene sequence containing random mutation protein was obtained by overlapping extension of PCR.The random mutation lipocalin library was constructed using phagemid expression vector.Lipocalin library was screened by subtracted screening of NSF60 cells and affinity screening of mast cells,and the lipocalin secondary library binding to mast cells was obtained.Then the lipocalin secondary library was enriched and screened with FcεRI-αreceptor protein as target molecule,and specific binding phages were eluted.After three rounds of screening,eight recombinant phage clones were randomly selected from elution clones of the third round.ELISA assay showed that three anticalin molecules could specifically bind to the FcεRI-αreceptor of mast cells.These results may provide some candidate biological molecules for the development of blocking drugs of mast cell FcεRI-αreceptor,and also lay the foundation for the development of biological small molecule drugs to treat Ig E associated allergic diseases.展开更多
A library of 2 ×107 random octapeptides was constructed by use of phagemid-based monovalent phage display system. The randomly synthesized degenerated oligodeoxyribonucleotides (oligos ) were fused to the truncat...A library of 2 ×107 random octapeptides was constructed by use of phagemid-based monovalent phage display system. The randomly synthesized degenerated oligodeoxyribonucleotides (oligos ) were fused to the truncated g Ⅲ (p230-p403). Sequence analysis of 11 randomly chosen clones suggested that the degenerated inserts and its deduced amino acid (aa) sequences are randomly distributed. The library was used to select binding peptides to the monoclonal antibody (mAb) 9E10, which recognizes a continuous decapeptide epi- tope of denatured human c-myc protein. After four to five rounds of panning, most of the eluted clones could bind to 9E10. Sequence analysis of the selected positive clones indicated that the binding sequences could fall into two classes, one class (clone 1) shares a consensus motif, ISE x x L, with c-myc decapeptide; and the sequences of the other class are entirely different. The binding of both classes to 9E10 could be specifically inhibited by free c-myc decapeptide. The immunogenicity of the phage peptide was further investigated by construction of multivalent displayed phage peptides and immunization of animals with or without adjuvant. ELISA and competitive ELISA showed that anti-serum from both mice and rabbit immunized with either clone could bind to the original antigen, c-myc decapeptide. These results denote that in spite of the dissimilarity of the selected peptides with c-myc decapeptide, they are capable of inducing similar immune respones in vivo, thus actually mimicking the antigen epitope.展开更多
As the sunlight scattered on the sea of Guanyinshan in Xiamen,a space that bridges creativity and industry opened its door to the world.On December 15th,following a ribbon-cutting and unveiling ceremony,YKK Xiamen Gua...As the sunlight scattered on the sea of Guanyinshan in Xiamen,a space that bridges creativity and industry opened its door to the world.On December 15th,following a ribbon-cutting and unveiling ceremony,YKK Xiamen Guanyinshan Showroom,the brand's second and largest comprehensive showroom,was officially inaugurated.This showroom is more than a product display window,it is an"Inspiration Hub"integrating exhibition,co-creation,and service.Its launch signifies YKK's progression in the Chinese market from“building an efficient product proposal system”to a new stage of“deep collaborative co-creation.”展开更多
Detector and event visualization are crucial components of high-energy physics(HEP)experimental software.Virtual reality(VR)technologies and multimedia development platforms,such as Unity,offer enhanced display effect...Detector and event visualization are crucial components of high-energy physics(HEP)experimental software.Virtual reality(VR)technologies and multimedia development platforms,such as Unity,offer enhanced display effects and flexible extensibility for visualization in HEP experiments.In this study,we present a VR-based method for detector and event displays in the Jiangmen Underground Neutrino Observatory(JUNO)experiment.This method shares the same detector geometry descriptions and event data model as those in the offline software and provides the necessary data conversion interfaces.The VR methodology facilitates an immersive exploration of the virtual environment in JUNO,enabling users to investigate the detector geometry,visualize event data,and tune the detector simulation and event reconstruction algorithms.Additionally,this approach supports applications in data monitoring,physics data analysis,and public outreach initiatives.展开更多
China’s 30 plus provinces,municipalities and autonomous regions take turns showcasing their culture and customs at the ongoing World Expo in Shanghai. Beijing took the lead by hosting Beijing Week in May. Since then,...China’s 30 plus provinces,municipalities and autonomous regions take turns showcasing their culture and customs at the ongoing World Expo in Shanghai. Beijing took the lead by hosting Beijing Week in May. Since then,many local arts have been featured,such as the New Year paintings of Tianjin’ s Yangliuqing Town,the pastoral songs of the Inner Mongolia Autonomous Region,the song-and-dance duet popular in northeast China and the acrobatics of Hebei’ s Wuqiao County.展开更多
Ford Motor,for the first time,displayed its globalproducts at the 8th Beijing Intemational Motor Show on June8 with its new generation Focus concept car making a globaldebut. The exhibits included the vehicles designe...Ford Motor,for the first time,displayed its globalproducts at the 8th Beijing Intemational Motor Show on June8 with its new generation Focus concept car making a globaldebut. The exhibits included the vehicles designed andmanufactured in North America,Europe and Australia.Ford,展开更多
Hepatitis B immunoglobulin （HBIG） is important in the management of hepatitis B virus （HBV） infection. Aiming to develop recombinant monoclona# antibodies as an alternative to HBIG, we report the successful identi...Hepatitis B immunoglobulin （HBIG） is important in the management of hepatitis B virus （HBV） infection. Aiming to develop recombinant monoclona# antibodies as an alternative to HBIG, we report the successful identification of HBV surface antigen （HBsAg）-specific antibodies from a full-length human antibody library displayed on mammalian cell surface. Using total RNA of peripheral blood mononuclear cells of a natively immunized donor as template, the antibody repertoire was amplified. Combining four-way ligation and the FIp recombinase-mediated integration （FIp-ln） system, we constructed a mammalian cell-based, fully human, full-length antibody display library in which each cell displayed only one kind of antibody molecule. By screening the cell library using fluorescence-activated cell sorting （FACS）, eight cell clones that displayed HBsAg-specific antibodies on cell surfaces were identified. DNA sequence analysis of the antibody genes revealed three unique antibodies. FACS data indicated that fluorescent strength of expression （FSE）, fluorescent strength of binding （FSB） and relative binding ability （RBA） were all different among them. These results demonstrated that by using our antibody mammalian display and screening platform, we can successfully identify antigen-specific antibodies from an immunized full-length antibody library. Therefore, this platform is very useful for the development of therapeutic antibodies.展开更多
Broad-spectrum antibacterial drugs often lack specificity,leading to indiscriminate bactericidal activity,which can disrupt the normal microbial balance of the host flora and cause unnecessary cytotoxicity during syst...Broad-spectrum antibacterial drugs often lack specificity,leading to indiscriminate bactericidal activity,which can disrupt the normal microbial balance of the host flora and cause unnecessary cytotoxicity during systemic administration.In this study,we constructed a specifically targeted antimicrobial peptide against Staphylococcus aureus by introducing a phage-displayed peptide onto a broad-spectrum antimicrobial peptide and explored its structure–function relationship through one-factor modification.SFK2 obtained by screening based on the selectivity index and the targeting index showed specific killing ability against S.aureus.Moreover,SFK2 showed excellent biocompatibility in mice and piglet,and demonstrated significant therapeutic efficacy against S.aureus infection.In conclusion,our screening of phage-derived heptapeptides effectively enhances the specific bactericidal ability of the antimicrobial peptides against S.aureus,providing a theoretical basis for developing targeted antimicrobial peptides.展开更多
Brain-derived neurotrophic factor (BDNF) shows potential in the treatment of neurode-generative diseases, but the therapeutic application of BDNF has been greatly limited because it is too large in molecular size to p...Brain-derived neurotrophic factor (BDNF) shows potential in the treatment of neurode-generative diseases, but the therapeutic application of BDNF has been greatly limited because it is too large in molecular size to permeate blood-brain barrier. To develop low-molecular-weight BDNF-like peptides, we selected a phage-displayed random peptide library using trkB expressed on NIH 3T3 cells as target in the study. With the strategy of peptide library incubation with NIH 3T3 cells and competitive elution with 1 mg/mL of BDNF in the last round of selection, the specific phages able to bind to the natural conformation of trkB and antagonize BDNF binding to trkB were enriched effectively. Five trkB-binding peptides were obtained, in which a core sequence of CRA/TXfXXfXXC (X represents the random amino acids, f represents T, L or I) was identified. The BDNF-like activity of these five peptides displayed on phages was not observed, though all of them antagonized the activity of BDNF in a dose-dependent manner. Similar results were obtained with the synthetic peptide of C1 clone, indicating that the 5 phage-derived peptides were trkB antagonists. These low-molecular-weight antagonists of trkB may be of potential application in the treatment of neuroblastoma and chronic pain. Meanwhile, the obtained core sequence also could be used as the base to construct the secondary phage-displayed peptide library for further devel-opment of small peptides mimicking BDNF activity.展开更多
The bacteriophage clones which can bind with shiga toxin B subunit (StxB) and inhibit cytotoxicity of shiga toxin were obtained by using antibody capturing method from a 15-mer random peptide library displayed on the ...The bacteriophage clones which can bind with shiga toxin B subunit (StxB) and inhibit cytotoxicity of shiga toxin were obtained by using antibody capturing method from a 15-mer random peptide library displayed on the surface of bacteriophage fd. Among them, one peptide encoded by the random DNA region of a selected bacteriophage (A12) was synthesized and tested in vitro and in vivo, where the peptide competed with the receptor of shiga toxin to bind StxB, and inhibited the cytotoxicity and enterotoxicity of shiga toxin. The peptide can also block other apparently unrelated StxB binding bacteriophage (A3), which suggests that there are overlapping StxB interaction sites for those ligands with different sequences. The results provide a demonstration of bacteriophage display to screen peptide ligands for a small and/or unable biotinylated molecule by antibodies-capturing strategy, and take the lead for the development of receptor antagonists for shiga toxin.展开更多
High-resolution non-emissive displays based on electrochromic tungsten oxides(WOx)are crucial for future near-eye virtual/augmented reality interactions,given their impressive attributes such as high environmental sta...High-resolution non-emissive displays based on electrochromic tungsten oxides(WOx)are crucial for future near-eye virtual/augmented reality interactions,given their impressive attributes such as high environmental stability,ideal outdoor readability,and low energy consumption.However,the limited intrinsic structure of inorganic materials has presented a significant challenge in achieving precise patterning/pixelation at the micron scale.Here,we successfully developed the direct photolithography for WOx nanoparticles based on in situ photo-induced ligand exchange.This strategy enabled us to achieve ultra-high resolution efficiently(line width<4μm,the best resolution for reported inorganic electrochromic materials).Additionally,the resulting device exhibited impressive electrochromic performance,such as fast response(<1 s at 0 V),high coloration efficiency(119.5 cm^(2) C^(−1)),good optical modulation(55.9%),and durability(>3600 cycles),as well as promising applications in electronic logos,pixelated displays,flexible electronics,etc.The success and advancements presented here are expected to inspire and accelerate research and development(R&D)in high-resolution non-emissive displays and other ultra-fine micro-electronics.展开更多
It is of great scientific significance to construct a 3D dynamic structural color with a special color effect based on the microlens array.However,the problems of imperfect mechanisms and poor color quality need to be...It is of great scientific significance to construct a 3D dynamic structural color with a special color effect based on the microlens array.However,the problems of imperfect mechanisms and poor color quality need to be solved.A method of 3D structural color turning on periodic metasurfaces fabricated by the microlens array and self-assembly technology was proposed in this study.In the experiment,Polydimethylsiloxane(PDMS)flexible film was used as a substrate,and SiO2 microspheres were scraped into grooves of the PDMS film to form 3D photonic crystal structures.By adjusting the number of blade-coated times and microsphere concentrations,high-saturation structural color micropatterns were obtained.These films were then matched with microlens arrays to produce dynamic graphics with iridescent effects.The results showed that by blade-coated two times and SiO2 microsphere concentrations of 50%are the best conditions.This method demonstrates the potential for being widely applied in the anticounterfeiting printing and ultra-high-resolution display.展开更多
文摘Prostate-specific membrane antigen (PSMA) is a cellsurface glycoprotein expressed predominantly in prostatesecretory acinar epithelium and prostate cancer cells aswell as in several extraprostatic tissues. Mouse monoclonal antibody 4G5 specific to the extracellular domainof PSMA was used to screen two phage displayed peptide libraries (9aa linear and 9aa cys library). Three 4G5reactive phagotopes were identified. Sequence analysis ofisolated clones demonstrated that the interaction motif'VDPA/SK' has high homology to 719-725aa on PSMA.Immunohistochemical staming of the prostate cancer sam ple with the PSMA-mimic phagotope (mimotope) immunized serum antibodies demonstrate that the mimotopeisolated from the phage displayed peptide libraries can induce PSMA specific immune response in vivo.
文摘BERNARDO Bertolucci’s film, The Last Emperor, drew its Western audience into a mystic, grandiose, oriental palace that was the Forbidden City, leaving them awestruck at the
基金The Science and Technology Project Foundation of Guangzhou,China(Grant No.201604020106)Natural Science Foundation of Guangdong Province(Grant No.1814050002837)
文摘The primers were designed according to the gene sequence of lipocalin protein family,and the gene sequence containing random mutation protein was obtained by overlapping extension of PCR.The random mutation lipocalin library was constructed using phagemid expression vector.Lipocalin library was screened by subtracted screening of NSF60 cells and affinity screening of mast cells,and the lipocalin secondary library binding to mast cells was obtained.Then the lipocalin secondary library was enriched and screened with FcεRI-αreceptor protein as target molecule,and specific binding phages were eluted.After three rounds of screening,eight recombinant phage clones were randomly selected from elution clones of the third round.ELISA assay showed that three anticalin molecules could specifically bind to the FcεRI-αreceptor of mast cells.These results may provide some candidate biological molecules for the development of blocking drugs of mast cell FcεRI-αreceptor,and also lay the foundation for the development of biological small molecule drugs to treat Ig E associated allergic diseases.
文摘A library of 2 ×107 random octapeptides was constructed by use of phagemid-based monovalent phage display system. The randomly synthesized degenerated oligodeoxyribonucleotides (oligos ) were fused to the truncated g Ⅲ (p230-p403). Sequence analysis of 11 randomly chosen clones suggested that the degenerated inserts and its deduced amino acid (aa) sequences are randomly distributed. The library was used to select binding peptides to the monoclonal antibody (mAb) 9E10, which recognizes a continuous decapeptide epi- tope of denatured human c-myc protein. After four to five rounds of panning, most of the eluted clones could bind to 9E10. Sequence analysis of the selected positive clones indicated that the binding sequences could fall into two classes, one class (clone 1) shares a consensus motif, ISE x x L, with c-myc decapeptide; and the sequences of the other class are entirely different. The binding of both classes to 9E10 could be specifically inhibited by free c-myc decapeptide. The immunogenicity of the phage peptide was further investigated by construction of multivalent displayed phage peptides and immunization of animals with or without adjuvant. ELISA and competitive ELISA showed that anti-serum from both mice and rabbit immunized with either clone could bind to the original antigen, c-myc decapeptide. These results denote that in spite of the dissimilarity of the selected peptides with c-myc decapeptide, they are capable of inducing similar immune respones in vivo, thus actually mimicking the antigen epitope.
文摘As the sunlight scattered on the sea of Guanyinshan in Xiamen,a space that bridges creativity and industry opened its door to the world.On December 15th,following a ribbon-cutting and unveiling ceremony,YKK Xiamen Guanyinshan Showroom,the brand's second and largest comprehensive showroom,was officially inaugurated.This showroom is more than a product display window,it is an"Inspiration Hub"integrating exhibition,co-creation,and service.Its launch signifies YKK's progression in the Chinese market from“building an efficient product proposal system”to a new stage of“deep collaborative co-creation.”
基金supported by the National Natural Science Foundation of China(Nos.12175321,W2443004,11975021,11675275,U1932101)Strategic Priority Research Program of the Chinese Academy of Sciences(No.XDA10010900)+2 种基金National Key Research and Development Program of China(Nos.2023YFA1606000 and 2020YFA0406400)National College Students Science and Technology Innovation ProjectUndergraduate Base Scientific Research Project of Sun Yat-sen University。
文摘Detector and event visualization are crucial components of high-energy physics(HEP)experimental software.Virtual reality(VR)technologies and multimedia development platforms,such as Unity,offer enhanced display effects and flexible extensibility for visualization in HEP experiments.In this study,we present a VR-based method for detector and event displays in the Jiangmen Underground Neutrino Observatory(JUNO)experiment.This method shares the same detector geometry descriptions and event data model as those in the offline software and provides the necessary data conversion interfaces.The VR methodology facilitates an immersive exploration of the virtual environment in JUNO,enabling users to investigate the detector geometry,visualize event data,and tune the detector simulation and event reconstruction algorithms.Additionally,this approach supports applications in data monitoring,physics data analysis,and public outreach initiatives.
文摘China’s 30 plus provinces,municipalities and autonomous regions take turns showcasing their culture and customs at the ongoing World Expo in Shanghai. Beijing took the lead by hosting Beijing Week in May. Since then,many local arts have been featured,such as the New Year paintings of Tianjin’ s Yangliuqing Town,the pastoral songs of the Inner Mongolia Autonomous Region,the song-and-dance duet popular in northeast China and the acrobatics of Hebei’ s Wuqiao County.
文摘Ford Motor,for the first time,displayed its globalproducts at the 8th Beijing Intemational Motor Show on June8 with its new generation Focus concept car making a globaldebut. The exhibits included the vehicles designed andmanufactured in North America,Europe and Australia.Ford,
文摘Hepatitis B immunoglobulin （HBIG） is important in the management of hepatitis B virus （HBV） infection. Aiming to develop recombinant monoclona# antibodies as an alternative to HBIG, we report the successful identification of HBV surface antigen （HBsAg）-specific antibodies from a full-length human antibody library displayed on mammalian cell surface. Using total RNA of peripheral blood mononuclear cells of a natively immunized donor as template, the antibody repertoire was amplified. Combining four-way ligation and the FIp recombinase-mediated integration （FIp-ln） system, we constructed a mammalian cell-based, fully human, full-length antibody display library in which each cell displayed only one kind of antibody molecule. By screening the cell library using fluorescence-activated cell sorting （FACS）, eight cell clones that displayed HBsAg-specific antibodies on cell surfaces were identified. DNA sequence analysis of the antibody genes revealed three unique antibodies. FACS data indicated that fluorescent strength of expression （FSE）, fluorescent strength of binding （FSB） and relative binding ability （RBA） were all different among them. These results demonstrated that by using our antibody mammalian display and screening platform, we can successfully identify antigen-specific antibodies from an immunized full-length antibody library. Therefore, this platform is very useful for the development of therapeutic antibodies.
基金supported by the National Key R&D Program of China(2022YFD1300404)the National Natural Science Foundation of China(31930106 and U22A20514)+1 种基金the 2115 Talent Development Program of China Agricultural University(1041-00109019)the Pinduoduo-China Agricultural University Research Fund(PC2023A01001).
文摘Broad-spectrum antibacterial drugs often lack specificity,leading to indiscriminate bactericidal activity,which can disrupt the normal microbial balance of the host flora and cause unnecessary cytotoxicity during systemic administration.In this study,we constructed a specifically targeted antimicrobial peptide against Staphylococcus aureus by introducing a phage-displayed peptide onto a broad-spectrum antimicrobial peptide and explored its structure–function relationship through one-factor modification.SFK2 obtained by screening based on the selectivity index and the targeting index showed specific killing ability against S.aureus.Moreover,SFK2 showed excellent biocompatibility in mice and piglet,and demonstrated significant therapeutic efficacy against S.aureus infection.In conclusion,our screening of phage-derived heptapeptides effectively enhances the specific bactericidal ability of the antimicrobial peptides against S.aureus,providing a theoretical basis for developing targeted antimicrobial peptides.
基金the National Natural Science Foundation of China (Grant Nos. 39825116 & 30070168). References
文摘Brain-derived neurotrophic factor (BDNF) shows potential in the treatment of neurode-generative diseases, but the therapeutic application of BDNF has been greatly limited because it is too large in molecular size to permeate blood-brain barrier. To develop low-molecular-weight BDNF-like peptides, we selected a phage-displayed random peptide library using trkB expressed on NIH 3T3 cells as target in the study. With the strategy of peptide library incubation with NIH 3T3 cells and competitive elution with 1 mg/mL of BDNF in the last round of selection, the specific phages able to bind to the natural conformation of trkB and antagonize BDNF binding to trkB were enriched effectively. Five trkB-binding peptides were obtained, in which a core sequence of CRA/TXfXXfXXC (X represents the random amino acids, f represents T, L or I) was identified. The BDNF-like activity of these five peptides displayed on phages was not observed, though all of them antagonized the activity of BDNF in a dose-dependent manner. Similar results were obtained with the synthetic peptide of C1 clone, indicating that the 5 phage-derived peptides were trkB antagonists. These low-molecular-weight antagonists of trkB may be of potential application in the treatment of neuroblastoma and chronic pain. Meanwhile, the obtained core sequence also could be used as the base to construct the secondary phage-displayed peptide library for further devel-opment of small peptides mimicking BDNF activity.
文摘The bacteriophage clones which can bind with shiga toxin B subunit (StxB) and inhibit cytotoxicity of shiga toxin were obtained by using antibody capturing method from a 15-mer random peptide library displayed on the surface of bacteriophage fd. Among them, one peptide encoded by the random DNA region of a selected bacteriophage (A12) was synthesized and tested in vitro and in vivo, where the peptide competed with the receptor of shiga toxin to bind StxB, and inhibited the cytotoxicity and enterotoxicity of shiga toxin. The peptide can also block other apparently unrelated StxB binding bacteriophage (A3), which suggests that there are overlapping StxB interaction sites for those ligands with different sequences. The results provide a demonstration of bacteriophage display to screen peptide ligands for a small and/or unable biotinylated molecule by antibodies-capturing strategy, and take the lead for the development of receptor antagonists for shiga toxin.
基金supported by the National Key R&D Program of China(2022YFB3606501,2022YFB3602902)the Key projects of National Natural Science Foundation of China(62234004)+8 种基金the National Natural Science Foundation of China(U23A2092)Pioneer and Leading Goose R&D Program of Zhejiang(2024C01191,2024C01092)Innovation and Entrepreneurship Team of Zhejiang Province(2021R01003)Ningbo Key Technologies R&D Program(2022Z085),Ningbo 3315 Programme(2020A-01-B)YONGJIANG Talent Introduction Programme(2021A-038-B,2021A-159-G)“Innovation Yongjiang 2035”Key R&D Programme(2024Z146)Ningbo JiangBei District public welfare science and technology project(2022C07)the China National Postdoctoral Program for Innovative Talents(grant no.BX20240391)the China Postdoctoral Science Foundation(grant no.2023M743623).
文摘High-resolution non-emissive displays based on electrochromic tungsten oxides(WOx)are crucial for future near-eye virtual/augmented reality interactions,given their impressive attributes such as high environmental stability,ideal outdoor readability,and low energy consumption.However,the limited intrinsic structure of inorganic materials has presented a significant challenge in achieving precise patterning/pixelation at the micron scale.Here,we successfully developed the direct photolithography for WOx nanoparticles based on in situ photo-induced ligand exchange.This strategy enabled us to achieve ultra-high resolution efficiently(line width<4μm,the best resolution for reported inorganic electrochromic materials).Additionally,the resulting device exhibited impressive electrochromic performance,such as fast response(<1 s at 0 V),high coloration efficiency(119.5 cm^(2) C^(−1)),good optical modulation(55.9%),and durability(>3600 cycles),as well as promising applications in electronic logos,pixelated displays,flexible electronics,etc.The success and advancements presented here are expected to inspire and accelerate research and development(R&D)in high-resolution non-emissive displays and other ultra-fine micro-electronics.
文摘It is of great scientific significance to construct a 3D dynamic structural color with a special color effect based on the microlens array.However,the problems of imperfect mechanisms and poor color quality need to be solved.A method of 3D structural color turning on periodic metasurfaces fabricated by the microlens array and self-assembly technology was proposed in this study.In the experiment,Polydimethylsiloxane(PDMS)flexible film was used as a substrate,and SiO2 microspheres were scraped into grooves of the PDMS film to form 3D photonic crystal structures.By adjusting the number of blade-coated times and microsphere concentrations,high-saturation structural color micropatterns were obtained.These films were then matched with microlens arrays to produce dynamic graphics with iridescent effects.The results showed that by blade-coated two times and SiO2 microsphere concentrations of 50%are the best conditions.This method demonstrates the potential for being widely applied in the anticounterfeiting printing and ultra-high-resolution display.
