A novel volumetric three-dimensional（3-D） display system is developed based on the human eye persistence and the system fuses a time-series of image slices into a single hologram like 3-D aerial image. The system de...A novel volumetric three-dimensional（3-D） display system is developed based on the human eye persistence and the system fuses a time-series of image slices into a single hologram like 3-D aerial image. The system design is introduced and key components are described. Experimental results show that the 3-D system can guide people freely walk around the display to inspect the true 3-D image without goggles.展开更多
Based on the helix rotating screen and the digital micro-mirror device （DMD）, the former proto of volumetric-swept display system is improved. The 3-D display system adopting a helix rotating screen to construct an ...Based on the helix rotating screen and the digital micro-mirror device （DMD）, the former proto of volumetric-swept display system is improved. The 3-D display system adopting a helix rotating screen to construct an imaging space meliorate the defects, such as the smaller image space, the fewer voxels and the severer voxel overlap dead zone caused by planar rotating screen. DMD with spatial light modular （SLM） technology increases the transmission bandwidth of 3-D data in the voxel activation subsystem and activate multi-voxel once time. The volumetric-swept system based on helix rotating screen and DMD is developed. The experimental results show that the image space, the vision dead zone, the voxels on slice, and the voxel activation capacity of the designed proto are superior to the plane rotating screen system.展开更多
Phage display technology is a unique gene recombination expression technology, and it is also a simple and effective screening tool. Through panning, a protein or peptide with high affinity and selectivity to the targ...Phage display technology is a unique gene recombination expression technology, and it is also a simple and effective screening tool. Through panning, a protein or peptide with high affinity and selectivity to the target is obtained. Antibody phage display has become the first and most widely used <i><span style="font-family:Verdana;">in vitro</span></i><span style="font-family:Verdana;"> screening technology. Phage display derivatives play an important role in the diagnosis and treatment of diseases. This article reviews the phage display system of phage display technology, the size and classification of antibody libraries and their applications, and discusses the application prospects and challenges of phage display technology.</span><span style="font-family:Verdana;"> </span><span style="font-family:Verdana;">This thesis lays the foundation for the theoretical and experimental research of bacteriophages.</span>展开更多
This paper presents the development of numerical prediction products(NPP) correction and display system(NPPCDS) for rapid and effective post-processing and displaying of the T213 NPP(numerical prediction products of t...This paper presents the development of numerical prediction products(NPP) correction and display system(NPPCDS) for rapid and effective post-processing and displaying of the T213 NPP(numerical prediction products of the medium range numerical weather prediction spectral model T213L31) through instant correction method. The NPPCDS consists of two modules: an automatic correction module and a graphical display module. The automatic correction module automatically corrects the T213 NPP at regularly scheduled time intervals, while the graphical display module interacts with users to display the T213 NPP and its correction results. The system helps forecasters extract the most relevant information at a quick glance without extensive post-processing. It is simple, easy to use, and computationally efficient, and has been running stably at Huludao Meteorological Bureau in Liaoning Province of China for the past three years. Because of its low computational costs, it is particularly useful for meteorological departments that lack advanced computing capacity and still need to make short-range weather forecasting.展开更多
To model a true three-dimensional(3D)display system,we introduced the method of voxel molding to obtain the stereoscopic imaging space of the system.For the distribution of each voxel,we proposed a four-dimensional(4D...To model a true three-dimensional(3D)display system,we introduced the method of voxel molding to obtain the stereoscopic imaging space of the system.For the distribution of each voxel,we proposed a four-dimensional(4D)Givone–Roessor(GR)model for state-space representation—that is,we established a local state-space model with the 3D position and one-dimensional time coordi-nates to describe the system.First,we extended the original elementary operation approach to a 4D condition and proposed the implementation steps of the realiza-tion matrix of the 4D GR model.Then,we described the working process of a true 3D display system,analyzed its real-time performance,introduced the fixed-point quantization model to simplify the system matrix,and derived the conditions for the global asymptotic stability of the system after quantization.Finally,we provided an example to prove the true 3D display system’s feasibility by simulation.The GR-model-representation method and its implementation steps proposed in this paper simplified the system’s mathematical expression and facilitated the microcon-troller software implementation.Real-time and stability analyses can be used widely to analyze and design true 3D display systems.展开更多
In this study, a multipurpose M13KE phage display vector was constructed from wild-type M13KE phage for long peptide or protein display libraries without helper phage to expand the scope of targeted high-throughput sc...In this study, a multipurpose M13KE phage display vector was constructed from wild-type M13KE phage for long peptide or protein display libraries without helper phage to expand the scope of targeted high-throughput screening. Based on the relationship between the structure and function of minor coat protein of wild-type MI3KE （wt-plII）, a truncated gene III （tglll） encoding minor coat protein from M13KE phage was cloned. A fusion gene fragment harboring a hw/tac promoter, signal peptide and C-terminal region sequence of gill was assembled with SOEing-PCR （splice-overlapping-extension polymerase chain reaction） method and inserted into M13KE vector. SDS-PAGE and Western blot analysis with anti-M13 pIII moneclonal antibody were employed to detect the expression of re- combinant protein, c-Myc and HA tag sequences were fused into the recombinant protein. The results showed that tglll was inserted into an unessential region of M13KE. According to the results of SDS-PAGE and Western blot with anti-M13 pIII antibody, pIII was expressed by wt-gIII and tgIII, glII harboring two tags ex- pressed both c-Myc and HA peptides using SDS-PAGE and Western blot with the corresponding monoclonal antibodies. In this study, a multipurpose M13KE phage display system was successfully constructed, which could express both short and long peptide libraries without helper phage. In future, the obtained M13KE phage display system may be used for targeted high-throughput screening of long peptide libraries without helper phage.展开更多
Rotavirus(RV) is a major foodborne pathogen. For RV prevention and control, it is a key to uncover the interaction mechanism between virus and its receptors. However, it is hard to specially purify the viral receptors...Rotavirus(RV) is a major foodborne pathogen. For RV prevention and control, it is a key to uncover the interaction mechanism between virus and its receptors. However, it is hard to specially purify the viral receptors, including histo-blood group antigens(HBGAs). Previously, the protruding domain protein(P protein) of human norovirus(genotype Ⅱ.4) was displayed on the surface of Escherichia coli, and it specifically recognized and captured the viral ligands. In order to further verify the feasibility of the system, P protein was replaced by VP8* of RV(G9 P[8]) in this study. In the system, VP8*could be correctly released by thrombin treatment with antigenicity retaining, which was confirmed by Western blot and Enzyme-Linked Immunosorbent Assays. Type A HBGAs from porcine gastric mucin(PGM) were recognized and captured by this system. From saliva mixture, the captured viral receptor bound with displayed VP8* was confirmed positive with monoclonal antibody against type A HBGAs. It indicated that the target ligands could be easily separated from the complex matrix. These results demonstrate that the bacterial surface display system will be an effective platform to explore viral receptors/ligands from cell lines or food matrix.展开更多
In this paper, a scalable hardware and software architecture for tiled display systems (a.k.a. videowalls), which can be implemented by using low-cost devices, together with a dynamic web-based management and configur...In this paper, a scalable hardware and software architecture for tiled display systems (a.k.a. videowalls), which can be implemented by using low-cost devices, together with a dynamic web-based management and configuration service are proposed. It has been designed to support both stored and live broadcast/broadband content, in mosaic or warp distributions. The displays and devices can be dynamically configured via web in different ways: the displays can create a single display of a larger size;or they can be configured in a customized way in order to playout different media contents in different display combinations. As display renderers, low-cost devices are proposed as the main hardware element to obtain affordable videowall systems. As a proof of concept, two prototypes have been implemented, including an accurate synchronization mechanism based on a Master/Slave control scheme and aggressive and smooth playout adjustment techniques. To evidence the good performance of the prototypes and configuration service, both objective and subjective evaluations have been conducted regarding synchronization accuracy and usability. On the one hand, the mean values of the asynchronies between the video playout processes in each display are kept below 25ms (i.e., frame accuracy). On the other hand, the obtained usability score in the System Usability Scale (SUS) test has been 88.65, which is considered as excellent.展开更多
Holography is an interesting tool in creating real objects and scenes which can be projected anywhere with accurate details and depth impression. It is also found to be more attractive to the artists than other altern...Holography is an interesting tool in creating real objects and scenes which can be projected anywhere with accurate details and depth impression. It is also found to be more attractive to the artists than other alternatives. For that reason, digital holography is being used as a display technology in cartoon movies. Since this application is dependent on the performance and the simplicity of the available display technology, it becomes very useful to improve the display technique in order to become fast, simple, and attractive by being combined with computer graphical effects. This paper discusses a simulation of a digital holographic model as a three dimensional (3D) display system and its application in making cartoon holography.展开更多
Microbial cell surface display technology is a recombinant technology to express target proteins on the cell membrane,which can be used to redesign the cell surface with functional proteins and peptides.Bacterial and ...Microbial cell surface display technology is a recombinant technology to express target proteins on the cell membrane,which can be used to redesign the cell surface with functional proteins and peptides.Bacterial and yeast surface display systems are the most common cell surface display systems of prokaryotic and eukaryotic proteins,that are widely applied as the core elements in the field of biosensors due to their advantages,including enhanced stability,high yield,good safety,expression of larger and more complex proteins.To further promote the performance of biosensors,the biomineralized microbial surface display technology was proposed.This review summarized the different microbial surface display systems and the biomineralized surface display systems,where the mechanisms of surface display and biomineralization were introduced.Then we described the recent progress of their applications on biosensors for different types of detection targets.Finally,the outlooks and tendencies were discussed and forecasted with the expectation to provide some general functions and enlightenments to this aspect of research.展开更多
In order to achieve a clear and steady swept-volume display,the method of swept-volume display based on cylindrical space projection was presented. One projector generated the image volume in π× 70 mm × 70 ...In order to achieve a clear and steady swept-volume display,the method of swept-volume display based on cylindrical space projection was presented. One projector generated the image volume in π× 70 mm × 70 mm × 150 mm space. Experimentally,the resolution of images was 800 pixel × 600 pixel × 360 pixel,which resulted in almost 345 million voxels. In order to achieve space voxels with uniform brightness, curved reflectors were also designed. In addition,the match conditions between triangles and the scanning planes in the volume space were classified and a sweptvolume graphics engine based on embedded platform was designed.The image rendering the hardware foundation for three-dimensional( 3D) dynamic images generation was achieved. Demonstrated in the experiments,light source utilization of the second-generation system based on curved mirror is about three times brighter than the firstgeneration 3D minitor based on flat mirror,and this system is able to display color,clear and well-proportioned 3D images in brighter room light.展开更多
By providing real-time updates of essential information, airports not only display and disseminate information but also help control the flow of traffic. In order to maximize available space, particularly in high traf...By providing real-time updates of essential information, airports not only display and disseminate information but also help control the flow of traffic. In order to maximize available space, particularly in high traffic areas, Airport Display Information Systems should be integrated into the overall design of the airport and their positioning should be carefully planned to deliver optimal results. Airport Display Information Systems can help airports maximize space, increase customer satisfaction, and generate new revenue opportunities. The technology is designed not only to comply with environmental regulations, but also to help airports keep budgets in check. This paper discusses airport display systems, their connections and interoperability with other systems and who the key airport users of these airport display systems are.展开更多
The advent of artificial intelligence(AI)has propelled augmented reality(AR)display technology to a pivotal juncture,positioning it as a contender for the next generation of mobile intelligent terminals.However,the pu...The advent of artificial intelligence(AI)has propelled augmented reality(AR)display technology to a pivotal juncture,positioning it as a contender for the next generation of mobile intelligent terminals.However,the pursuit of advanced AR displays,particularly those capable of delivering immersive 3D experiences,is significantly hindered by the performance limitations of current hardware and the complexity of system integration.In this study,we present an innovative multi-focal plane AR display system that integrates a non-orthogonal polarization-multiplexing metasurface,freeform optical elements,and an OLED display screen.All optical elements are integrated into a single solid-state architecture,based on a joint optimization design approach of ray tracing and diffraction theory.The multi-focal plane AR visual effect is realized by the compact and multiplexing metasurface,which performs distinct phase functions across diverse polarization channels.Meanwhile,freeform surfaces offer ample design flexibility for the collaborative optimization of multi-focal plane imaging and the see-through systems.Followed by a mechanical design and prototype assembly,we demonstrate the system's capabilities in real-time and multi-focal plane display.The digital images at all virtual image distances seamlessly integrate with the real environment,fully exhibiting the system's high parallelism and real-time interactivity.With the innovative design concept and joint design method,we believe that our work will spur more innovative and compact intelligent solutions for AR displays and inject new vitality into hybrid optical systems.展开更多
Neutralizing antibodies are essential tools in antiviral therapy and epidemic preparedness,capable of directlyinhibiting viral entry and limiting disease progression.However,traditional antibody discovery strategies—...Neutralizing antibodies are essential tools in antiviral therapy and epidemic preparedness,capable of directlyinhibiting viral entry and limiting disease progression.However,traditional antibody discovery strategies—suchas animal immunization or B cell isolation from infected individuals—are often hindered by biosafety concerns,lengthy development timelines,and limited adaptability during outbreaks.In the present study,we aimed toestablish a robust and rapid in vitro platform for the efficient isolation of neutralizing antibodies targetingconserved viral epitopes.We developed an epitope-guided negative screening strategy that integrates phagedisplay technology with rational antigen mutagenesis to exclude antibodies against variable regions whileenriching for those that recognize functionally constrained epitopes.When applied to the receptor-binding domainof severe acute respiratory syndrome coronavirus 2,this method enabled the identification of six neutralizingantibodies(one IgG and five nanobodies)exhibiting broad-spectrum neutralizing activity across multiple viralvariants.Notably,antibodies recognizing distinct epitopes demonstrated significant synergistic neutralizationwhen used in combination(P<0.05).This screening approach facilitates the rapid discovery of potent andmutation-resistant antibodies and holds promise for application to other emerging pathogens.Our findingsunderscore the potential of epitope-guided,in vitro platforms in expediting therapeutic antibody developmentunder conditions of high biosafety requirements.展开更多
Holographic 3D display technology,widely considered the ultimate solution for real 3D display,has broad applications in fields including advertisement,industrial manufacturing and military.However,it is difficult to s...Holographic 3D display technology,widely considered the ultimate solution for real 3D display,has broad applications in fields including advertisement,industrial manufacturing and military.However,it is difficult to simultaneously realize color holographic 3D display with wide viewing angle and high brightness required for an immersive visual experience.Here,a novel holographic 3D display system based on a customized achromatic liquid crystal grating and a phase-only spatial light modulator is proposed.Thanks to the secondary diffraction performed by the achromatic liquid crystal grating,nine secondary diffraction images of red,green and blue channels overlap in space in time sequence.Additionally,a high brightness hologram encoding method is developed,which introduces a frequency loss function with dynamic weights to ensure that differences of all frequency components in the frequency domain can be learned.The proposed method dramatically enhances light energy utilization by a factor of five,resulting in significantly brighter reconstructed images while substantially attenuating background noise in non-target regions.This groundbreaking system,achieving a remarkable~65°wide viewing angle with good image quality and high brightness,represents a significant advancement in holographic technology,offering a comprehensive solution for wide-viewing-angle,high-brightness,color 3D displays with potential applications across diverse technological domains.展开更多
The near-eye display feature in emerging spatial computing systems produces a distinctive visual effect of mixing virtual and real worlds.However,its application for all-day wear is greatly limited by the bulky struct...The near-eye display feature in emerging spatial computing systems produces a distinctive visual effect of mixing virtual and real worlds.However,its application for all-day wear is greatly limited by the bulky structure,energy expenditure,and continuous battery heating.Here,we propose a lightweight holographic near-eye display system that takes advantage of solar energy for self-charging.To guarantee the collection of solar energy and near-eye display without crosstalk,we implement holographic optical elements(HOEs)to diffract sunlight and signal light into a common waveguide.Then,small-area solar cells convert the collected solar energy and power the system.Compact power supply components replace heavy batteries,thus contributing to the lightweight design.The simple acquisition and management of solar energy provide the system with sustainable self-charging capability.We believe that the lightweight design and continuous energy input solution will significantly promote the popularity of near-eye display in our daily lives.展开更多
In an era dominated by visual information,the display interface serves as a critical gateway between the human and digital worlds.The relentless pursuit of visual immersion has driven display technology from cinema sc...In an era dominated by visual information,the display interface serves as a critical gateway between the human and digital worlds.The relentless pursuit of visual immersion has driven display technology from cinema screens to smart-phones and now to virtual and augmented reality(VR/AR)headsets,progressively moving closer to the human eye.This evolution places unprecedented demands on pixel density,power efficiency,and form factor,pushing up against funda-mental physical and physiological limits.展开更多
Head-up displays(HUDs)are emerging as key components of intelligent vehicles,requiring wide-depth,large-area,and high-efficiency dynamic imaging,which remains difficult to realize with traditional refractive optics.Co...Head-up displays(HUDs)are emerging as key components of intelligent vehicles,requiring wide-depth,large-area,and high-efficiency dynamic imaging,which remains difficult to realize with traditional refractive optics.Computer-generated holography(CGH)with diffraction optics offers a promising solution to these technical demands.However,CGH optimization based on the fast Fourier transform(FFT)faces limitations such as zero-padding redundancy,coupled sampling intervals,and incompatible near-and farfield propagation models.Here,we report a holography-based multiplane HUD using a matrix multiplication(MM)-assisted diffraction algorithm that restructures the Fresnel integral into two sequential matrix operations,thus eliminating zero-padding and enabling fully decoupled sampling between object and image planes.Compared with FFT-based angular spectrum methods,the MM approach significantly improves computational speed and memory efficiency for hologram design,which is validated by demonstrating dual-plane holography with a size ratio exceeding 100:1 and unified reconstruction across Fresnel and Fraunhofer regimes within a single computation.A prototype HUD system is demonstrated successfully to exhibit multiple-plane holographic virtual images that can be mixed with real-world objects at three independent planes.The technique might be one of the potential candidates for next-generation intelligent vehicle displays.展开更多
White Cyphochilus insulanus beetles,exhibiting both environmental camouflage display and radiative cooling functions,serve as a good prototype for biomimetic fabrication.As inspired,this work presents a femtosecond(fs...White Cyphochilus insulanus beetles,exhibiting both environmental camouflage display and radiative cooling functions,serve as a good prototype for biomimetic fabrication.As inspired,this work presents a femtosecond(fs)laser-based biomimetic fabrication strategy that takes full use of the synthesized radiative cooling nanomaterials for a groundbreaking stimuli-responsive infrared(IR)impressionistic camouflage display.The proposed technique is capable of readily transforming various substrates(quartz glass and metals including Ti,Al,Zr,and W)into self-assembled porous networks(aerogels)consisting of oxygen-vacancy-rich oxide nanoparticles.Surprisingly,the emissions of all as-prepared porous particle-networks in the radiative-cooling long-wavelength infrared(LWIR)band are above 95%,with the SiO_(2) aerogels reaching a maximum of 99.6%.Benefiting from the far-from-equilibrium thermodynamic kinetics,metastable phases of anatase TiO_(2),tetragonal zirconia(t-ZrO_(2)),and monoclinic WO_(3)(Pc)are synthesizable,opening up opportunities for exploring their optical applications.Taking the low-temperature metastable phase WO_(3)(Pc)as representative for systematic studies,it is found that(1)the ratio WO_(3)(Pc)phase to that of room-temperature phase of WO_(3)(P2_(1)/n)can be tailored by modulation of processing parameters;(2)laser synthesized aerogels with hybrid phases of WO_(3)(Pc)and WO_(3)(P2_(1)/n)have a brighter visible whiteness,higher visible/nearinfrared(NIR)spectral selectivity than the natural prototype of white Cyphochilus insulanus beetles but with comparable LWIR emittance.White WO_(3) aerogel in situ deposited during flexibly fs laser artistic patterning can blur the painting features due to its radiative cooling effect,allowing a colorful impressionistic IR display in the heating mode.What's more,invisible painting features concealed by the white deposited WO_(3) aerogel are clearly/faintly distinguishable by introducing external stimuli of a human hand and sample heating,respectively,catalyzing progress in optical encryption and selectively stimuli-responsive decryption display in the infrared band.展开更多
This paper describes a virtual environment, which can present dynamic force transformation during the control of objects. A 5-DOF haptic interface with the capability to generate kinesthetic effect is combined. In thi...This paper describes a virtual environment, which can present dynamic force transformation during the control of objects. A 5-DOF haptic interface with the capability to generate kinesthetic effect is combined. In this system, the operator manipulates an object in a virtual environment by using the 5-DOF master arm. When contacting with the virtual object, the contact force can be calculated and shown in the graphic interface. The contact response and deformation of the virtual object, which are usually called haptic rendering, also can be performed. The study supplies an approach to improve the operator’s immersion and can be used in many tele-robot control fields.展开更多
文摘A novel volumetric three-dimensional（3-D） display system is developed based on the human eye persistence and the system fuses a time-series of image slices into a single hologram like 3-D aerial image. The system design is introduced and key components are described. Experimental results show that the 3-D system can guide people freely walk around the display to inspect the true 3-D image without goggles.
基金Supported by the National High Technology Research and Development Program of China(″863″Program)(2007AA01Z338)the National Science Foundation for Post-doctoral Scientists of China(20080441051)the Jiangsu Province Science Foundation for Post-doctoral Scientists(0802014c)~~
文摘Based on the helix rotating screen and the digital micro-mirror device （DMD）, the former proto of volumetric-swept display system is improved. The 3-D display system adopting a helix rotating screen to construct an imaging space meliorate the defects, such as the smaller image space, the fewer voxels and the severer voxel overlap dead zone caused by planar rotating screen. DMD with spatial light modular （SLM） technology increases the transmission bandwidth of 3-D data in the voxel activation subsystem and activate multi-voxel once time. The volumetric-swept system based on helix rotating screen and DMD is developed. The experimental results show that the image space, the vision dead zone, the voxels on slice, and the voxel activation capacity of the designed proto are superior to the plane rotating screen system.
文摘Phage display technology is a unique gene recombination expression technology, and it is also a simple and effective screening tool. Through panning, a protein or peptide with high affinity and selectivity to the target is obtained. Antibody phage display has become the first and most widely used <i><span style="font-family:Verdana;">in vitro</span></i><span style="font-family:Verdana;"> screening technology. Phage display derivatives play an important role in the diagnosis and treatment of diseases. This article reviews the phage display system of phage display technology, the size and classification of antibody libraries and their applications, and discusses the application prospects and challenges of phage display technology.</span><span style="font-family:Verdana;"> </span><span style="font-family:Verdana;">This thesis lays the foundation for the theoretical and experimental research of bacteriophages.</span>
基金Under the auspices of National Natural Science Foundation of China(No.91125010)
文摘This paper presents the development of numerical prediction products(NPP) correction and display system(NPPCDS) for rapid and effective post-processing and displaying of the T213 NPP(numerical prediction products of the medium range numerical weather prediction spectral model T213L31) through instant correction method. The NPPCDS consists of two modules: an automatic correction module and a graphical display module. The automatic correction module automatically corrects the T213 NPP at regularly scheduled time intervals, while the graphical display module interacts with users to display the T213 NPP and its correction results. The system helps forecasters extract the most relevant information at a quick glance without extensive post-processing. It is simple, easy to use, and computationally efficient, and has been running stably at Huludao Meteorological Bureau in Liaoning Province of China for the past three years. Because of its low computational costs, it is particularly useful for meteorological departments that lack advanced computing capacity and still need to make short-range weather forecasting.
基金This work was supported by the Key Research and Development Projects of Science and Technology Development Plan of Jilin Provincial Department of Science and Technology(20180201090gx).
文摘To model a true three-dimensional(3D)display system,we introduced the method of voxel molding to obtain the stereoscopic imaging space of the system.For the distribution of each voxel,we proposed a four-dimensional(4D)Givone–Roessor(GR)model for state-space representation—that is,we established a local state-space model with the 3D position and one-dimensional time coordi-nates to describe the system.First,we extended the original elementary operation approach to a 4D condition and proposed the implementation steps of the realiza-tion matrix of the 4D GR model.Then,we described the working process of a true 3D display system,analyzed its real-time performance,introduced the fixed-point quantization model to simplify the system matrix,and derived the conditions for the global asymptotic stability of the system after quantization.Finally,we provided an example to prove the true 3D display system’s feasibility by simulation.The GR-model-representation method and its implementation steps proposed in this paper simplified the system’s mathematical expression and facilitated the microcon-troller software implementation.Real-time and stability analyses can be used widely to analyze and design true 3D display systems.
基金Supported by Youth Fund of Suzhou Chien-shiung Institute of Technology(2013QNJJ38)
文摘In this study, a multipurpose M13KE phage display vector was constructed from wild-type M13KE phage for long peptide or protein display libraries without helper phage to expand the scope of targeted high-throughput screening. Based on the relationship between the structure and function of minor coat protein of wild-type MI3KE （wt-plII）, a truncated gene III （tglll） encoding minor coat protein from M13KE phage was cloned. A fusion gene fragment harboring a hw/tac promoter, signal peptide and C-terminal region sequence of gill was assembled with SOEing-PCR （splice-overlapping-extension polymerase chain reaction） method and inserted into M13KE vector. SDS-PAGE and Western blot analysis with anti-M13 pIII moneclonal antibody were employed to detect the expression of re- combinant protein, c-Myc and HA tag sequences were fused into the recombinant protein. The results showed that tglll was inserted into an unessential region of M13KE. According to the results of SDS-PAGE and Western blot with anti-M13 pIII antibody, pIII was expressed by wt-gIII and tgIII, glII harboring two tags ex- pressed both c-Myc and HA peptides using SDS-PAGE and Western blot with the corresponding monoclonal antibodies. In this study, a multipurpose M13KE phage display system was successfully constructed, which could express both short and long peptide libraries without helper phage. In future, the obtained M13KE phage display system may be used for targeted high-throughput screening of long peptide libraries without helper phage.
基金the National Key Research and Development Program of China(2017YFF0210200)the National Natural Science Foundation of China(31772078).
文摘Rotavirus(RV) is a major foodborne pathogen. For RV prevention and control, it is a key to uncover the interaction mechanism between virus and its receptors. However, it is hard to specially purify the viral receptors, including histo-blood group antigens(HBGAs). Previously, the protruding domain protein(P protein) of human norovirus(genotype Ⅱ.4) was displayed on the surface of Escherichia coli, and it specifically recognized and captured the viral ligands. In order to further verify the feasibility of the system, P protein was replaced by VP8* of RV(G9 P[8]) in this study. In the system, VP8*could be correctly released by thrombin treatment with antigenicity retaining, which was confirmed by Western blot and Enzyme-Linked Immunosorbent Assays. Type A HBGAs from porcine gastric mucin(PGM) were recognized and captured by this system. From saliva mixture, the captured viral receptor bound with displayed VP8* was confirmed positive with monoclonal antibody against type A HBGAs. It indicated that the target ligands could be easily separated from the complex matrix. These results demonstrate that the bacterial surface display system will be an effective platform to explore viral receptors/ligands from cell lines or food matrix.
基金partially funded by "Vicerrectorado de Investigación de la Universitat Politècnica de València" under projects with references PAID-1121 and PAID-12-21
文摘In this paper, a scalable hardware and software architecture for tiled display systems (a.k.a. videowalls), which can be implemented by using low-cost devices, together with a dynamic web-based management and configuration service are proposed. It has been designed to support both stored and live broadcast/broadband content, in mosaic or warp distributions. The displays and devices can be dynamically configured via web in different ways: the displays can create a single display of a larger size;or they can be configured in a customized way in order to playout different media contents in different display combinations. As display renderers, low-cost devices are proposed as the main hardware element to obtain affordable videowall systems. As a proof of concept, two prototypes have been implemented, including an accurate synchronization mechanism based on a Master/Slave control scheme and aggressive and smooth playout adjustment techniques. To evidence the good performance of the prototypes and configuration service, both objective and subjective evaluations have been conducted regarding synchronization accuracy and usability. On the one hand, the mean values of the asynchronies between the video playout processes in each display are kept below 25ms (i.e., frame accuracy). On the other hand, the obtained usability score in the System Usability Scale (SUS) test has been 88.65, which is considered as excellent.
文摘Holography is an interesting tool in creating real objects and scenes which can be projected anywhere with accurate details and depth impression. It is also found to be more attractive to the artists than other alternatives. For that reason, digital holography is being used as a display technology in cartoon movies. Since this application is dependent on the performance and the simplicity of the available display technology, it becomes very useful to improve the display technique in order to become fast, simple, and attractive by being combined with computer graphical effects. This paper discusses a simulation of a digital holographic model as a three dimensional (3D) display system and its application in making cartoon holography.
基金the National Natural Science Foundation of China(Grant No.21705087)Youth Innovation Team Project for Talent Introduction and Cultivation in Universities of Shandong Province(096-1622002)+2 种基金Research Foundation for Distinguished Scholars of Qingdao Agricultural University(663-1117015)the Postgraduate Innovation Program of Qingdao Agricultural University(QNYCX21069)the National Innovation Training Program for College Students(No.202210435030).
文摘Microbial cell surface display technology is a recombinant technology to express target proteins on the cell membrane,which can be used to redesign the cell surface with functional proteins and peptides.Bacterial and yeast surface display systems are the most common cell surface display systems of prokaryotic and eukaryotic proteins,that are widely applied as the core elements in the field of biosensors due to their advantages,including enhanced stability,high yield,good safety,expression of larger and more complex proteins.To further promote the performance of biosensors,the biomineralized microbial surface display technology was proposed.This review summarized the different microbial surface display systems and the biomineralized surface display systems,where the mechanisms of surface display and biomineralization were introduced.Then we described the recent progress of their applications on biosensors for different types of detection targets.Finally,the outlooks and tendencies were discussed and forecasted with the expectation to provide some general functions and enlightenments to this aspect of research.
基金Shanghai Committee of Science and Technology,China(No.14511108200)
文摘In order to achieve a clear and steady swept-volume display,the method of swept-volume display based on cylindrical space projection was presented. One projector generated the image volume in π× 70 mm × 70 mm × 150 mm space. Experimentally,the resolution of images was 800 pixel × 600 pixel × 360 pixel,which resulted in almost 345 million voxels. In order to achieve space voxels with uniform brightness, curved reflectors were also designed. In addition,the match conditions between triangles and the scanning planes in the volume space were classified and a sweptvolume graphics engine based on embedded platform was designed.The image rendering the hardware foundation for three-dimensional( 3D) dynamic images generation was achieved. Demonstrated in the experiments,light source utilization of the second-generation system based on curved mirror is about three times brighter than the firstgeneration 3D minitor based on flat mirror,and this system is able to display color,clear and well-proportioned 3D images in brighter room light.
文摘By providing real-time updates of essential information, airports not only display and disseminate information but also help control the flow of traffic. In order to maximize available space, particularly in high traffic areas, Airport Display Information Systems should be integrated into the overall design of the airport and their positioning should be carefully planned to deliver optimal results. Airport Display Information Systems can help airports maximize space, increase customer satisfaction, and generate new revenue opportunities. The technology is designed not only to comply with environmental regulations, but also to help airports keep budgets in check. This paper discusses airport display systems, their connections and interoperability with other systems and who the key airport users of these airport display systems are.
基金funding provided by National Natural Science Foundation of China(U21A20140)National Key Research and Development Program of China(2021YFA1401200)+2 种基金Beijing Natural Science Foundation(JQ24028)Beijing Nova Program(20240484557)Synergetic Extreme Condition User Facility(SECUF).
文摘The advent of artificial intelligence(AI)has propelled augmented reality(AR)display technology to a pivotal juncture,positioning it as a contender for the next generation of mobile intelligent terminals.However,the pursuit of advanced AR displays,particularly those capable of delivering immersive 3D experiences,is significantly hindered by the performance limitations of current hardware and the complexity of system integration.In this study,we present an innovative multi-focal plane AR display system that integrates a non-orthogonal polarization-multiplexing metasurface,freeform optical elements,and an OLED display screen.All optical elements are integrated into a single solid-state architecture,based on a joint optimization design approach of ray tracing and diffraction theory.The multi-focal plane AR visual effect is realized by the compact and multiplexing metasurface,which performs distinct phase functions across diverse polarization channels.Meanwhile,freeform surfaces offer ample design flexibility for the collaborative optimization of multi-focal plane imaging and the see-through systems.Followed by a mechanical design and prototype assembly,we demonstrate the system's capabilities in real-time and multi-focal plane display.The digital images at all virtual image distances seamlessly integrate with the real environment,fully exhibiting the system's high parallelism and real-time interactivity.With the innovative design concept and joint design method,we believe that our work will spur more innovative and compact intelligent solutions for AR displays and inject new vitality into hybrid optical systems.
基金supported by the Natural Science Foundation of the Jiangsu Higher Education Institutions of China(Grant No.22KJB310001 to W.G.)the Special Project of the Jiangsu Provincial Department of Science and Technology(Grant No.BE2023603 to W.G.)the Nanjing Medical University Science and Technology Development Foundation(Grant No.NMUB20210006 to W.G.).
文摘Neutralizing antibodies are essential tools in antiviral therapy and epidemic preparedness,capable of directlyinhibiting viral entry and limiting disease progression.However,traditional antibody discovery strategies—suchas animal immunization or B cell isolation from infected individuals—are often hindered by biosafety concerns,lengthy development timelines,and limited adaptability during outbreaks.In the present study,we aimed toestablish a robust and rapid in vitro platform for the efficient isolation of neutralizing antibodies targetingconserved viral epitopes.We developed an epitope-guided negative screening strategy that integrates phagedisplay technology with rational antigen mutagenesis to exclude antibodies against variable regions whileenriching for those that recognize functionally constrained epitopes.When applied to the receptor-binding domainof severe acute respiratory syndrome coronavirus 2,this method enabled the identification of six neutralizingantibodies(one IgG and five nanobodies)exhibiting broad-spectrum neutralizing activity across multiple viralvariants.Notably,antibodies recognizing distinct epitopes demonstrated significant synergistic neutralizationwhen used in combination(P<0.05).This screening approach facilitates the rapid discovery of potent andmutation-resistant antibodies and holds promise for application to other emerging pathogens.Our findingsunderscore the potential of epitope-guided,in vitro platforms in expediting therapeutic antibody developmentunder conditions of high biosafety requirements.
基金supported by the National Natural Science Foundation of China under Grant Nos:U22A2079,U21B2034,62275009,623B2008 and 62175006.
文摘Holographic 3D display technology,widely considered the ultimate solution for real 3D display,has broad applications in fields including advertisement,industrial manufacturing and military.However,it is difficult to simultaneously realize color holographic 3D display with wide viewing angle and high brightness required for an immersive visual experience.Here,a novel holographic 3D display system based on a customized achromatic liquid crystal grating and a phase-only spatial light modulator is proposed.Thanks to the secondary diffraction performed by the achromatic liquid crystal grating,nine secondary diffraction images of red,green and blue channels overlap in space in time sequence.Additionally,a high brightness hologram encoding method is developed,which introduces a frequency loss function with dynamic weights to ensure that differences of all frequency components in the frequency domain can be learned.The proposed method dramatically enhances light energy utilization by a factor of five,resulting in significantly brighter reconstructed images while substantially attenuating background noise in non-target regions.This groundbreaking system,achieving a remarkable~65°wide viewing angle with good image quality and high brightness,represents a significant advancement in holographic technology,offering a comprehensive solution for wide-viewing-angle,high-brightness,color 3D displays with potential applications across diverse technological domains.
基金National Natural Science Foundation of China(U22A2079,62035003,61975014)Beijing Municipal Science and Technology Commission,Adminitrative Commission of Zhongguancun Science Park(Z211100004821012).
文摘The near-eye display feature in emerging spatial computing systems produces a distinctive visual effect of mixing virtual and real worlds.However,its application for all-day wear is greatly limited by the bulky structure,energy expenditure,and continuous battery heating.Here,we propose a lightweight holographic near-eye display system that takes advantage of solar energy for self-charging.To guarantee the collection of solar energy and near-eye display without crosstalk,we implement holographic optical elements(HOEs)to diffract sunlight and signal light into a common waveguide.Then,small-area solar cells convert the collected solar energy and power the system.Compact power supply components replace heavy batteries,thus contributing to the lightweight design.The simple acquisition and management of solar energy provide the system with sustainable self-charging capability.We believe that the lightweight design and continuous energy input solution will significantly promote the popularity of near-eye display in our daily lives.
基金supported by the National Natural Science Foundation of China(Grant No.22105106)the Jiangsu Youth Science and Technology Talent Support Program(Grant No.JSTJ-2025-063)+1 种基金Nanjing Science and Technology Innovation Project for Overseas Students(Grant No.NJKCZYZZ2022-05)Start-up Funding from NUPTSF(Grant No.NY221003).
文摘In an era dominated by visual information,the display interface serves as a critical gateway between the human and digital worlds.The relentless pursuit of visual immersion has driven display technology from cinema screens to smart-phones and now to virtual and augmented reality(VR/AR)headsets,progressively moving closer to the human eye.This evolution places unprecedented demands on pixel density,power efficiency,and form factor,pushing up against funda-mental physical and physiological limits.
基金supported by the National Key Research and Development Program of China(Grant No.2022YFB3607300)the National Natural Science Foundation of China(Grant Nos.62322512,62225506,and 12134013)+7 种基金the Fundamental Research Funds for the Central Universities(Grant Nos.WK2030000108 and WK2030000090)the CAS Project for Young Scientists in Basic Research(Grant No.YSBR-049)supported by the National Natural Science Foundation of China(Grant Nos.12174260 and 12574326)the Shanghai Rising-Star Program(Grant No.21QA1406400)the Shanghai Science and Technology Development Fund(Grant Nos.21ZR1443500 and 21ZR1443600)the support from the China Postdoctoral Science Foundation(Grant No.2023M743364)support from the Center for Micro and Nanoscale Research and Fabrication,University of Science and Technology of Chinasupported by the UPOLabs,which provided the experimental and technical support。
文摘Head-up displays(HUDs)are emerging as key components of intelligent vehicles,requiring wide-depth,large-area,and high-efficiency dynamic imaging,which remains difficult to realize with traditional refractive optics.Computer-generated holography(CGH)with diffraction optics offers a promising solution to these technical demands.However,CGH optimization based on the fast Fourier transform(FFT)faces limitations such as zero-padding redundancy,coupled sampling intervals,and incompatible near-and farfield propagation models.Here,we report a holography-based multiplane HUD using a matrix multiplication(MM)-assisted diffraction algorithm that restructures the Fresnel integral into two sequential matrix operations,thus eliminating zero-padding and enabling fully decoupled sampling between object and image planes.Compared with FFT-based angular spectrum methods,the MM approach significantly improves computational speed and memory efficiency for hologram design,which is validated by demonstrating dual-plane holography with a size ratio exceeding 100:1 and unified reconstruction across Fresnel and Fraunhofer regimes within a single computation.A prototype HUD system is demonstrated successfully to exhibit multiple-plane holographic virtual images that can be mixed with real-world objects at three independent planes.The technique might be one of the potential candidates for next-generation intelligent vehicle displays.
基金financial support received from the Shanghai Pujiang Program(23PJ1406500)。
文摘White Cyphochilus insulanus beetles,exhibiting both environmental camouflage display and radiative cooling functions,serve as a good prototype for biomimetic fabrication.As inspired,this work presents a femtosecond(fs)laser-based biomimetic fabrication strategy that takes full use of the synthesized radiative cooling nanomaterials for a groundbreaking stimuli-responsive infrared(IR)impressionistic camouflage display.The proposed technique is capable of readily transforming various substrates(quartz glass and metals including Ti,Al,Zr,and W)into self-assembled porous networks(aerogels)consisting of oxygen-vacancy-rich oxide nanoparticles.Surprisingly,the emissions of all as-prepared porous particle-networks in the radiative-cooling long-wavelength infrared(LWIR)band are above 95%,with the SiO_(2) aerogels reaching a maximum of 99.6%.Benefiting from the far-from-equilibrium thermodynamic kinetics,metastable phases of anatase TiO_(2),tetragonal zirconia(t-ZrO_(2)),and monoclinic WO_(3)(Pc)are synthesizable,opening up opportunities for exploring their optical applications.Taking the low-temperature metastable phase WO_(3)(Pc)as representative for systematic studies,it is found that(1)the ratio WO_(3)(Pc)phase to that of room-temperature phase of WO_(3)(P2_(1)/n)can be tailored by modulation of processing parameters;(2)laser synthesized aerogels with hybrid phases of WO_(3)(Pc)and WO_(3)(P2_(1)/n)have a brighter visible whiteness,higher visible/nearinfrared(NIR)spectral selectivity than the natural prototype of white Cyphochilus insulanus beetles but with comparable LWIR emittance.White WO_(3) aerogel in situ deposited during flexibly fs laser artistic patterning can blur the painting features due to its radiative cooling effect,allowing a colorful impressionistic IR display in the heating mode.What's more,invisible painting features concealed by the white deposited WO_(3) aerogel are clearly/faintly distinguishable by introducing external stimuli of a human hand and sample heating,respectively,catalyzing progress in optical encryption and selectively stimuli-responsive decryption display in the infrared band.
文摘This paper describes a virtual environment, which can present dynamic force transformation during the control of objects. A 5-DOF haptic interface with the capability to generate kinesthetic effect is combined. In this system, the operator manipulates an object in a virtual environment by using the 5-DOF master arm. When contacting with the virtual object, the contact force can be calculated and shown in the graphic interface. The contact response and deformation of the virtual object, which are usually called haptic rendering, also can be performed. The study supplies an approach to improve the operator’s immersion and can be used in many tele-robot control fields.
