Depression is a common and severely debilitating neuropsychiatric disorder.Multiple studies indicate a strong correlation between the occurrence of immunological inflammation and the presence of depression.The basolat...Depression is a common and severely debilitating neuropsychiatric disorder.Multiple studies indicate a strong correlation between the occurrence of immunological inflammation and the presence of depression.The basolateral amygdala(BLA)is crucial in the cognitive and physiological processing and control of emotion.However,due to the lack of detection tools,the neural activity of the BLA during depression is not well understood.In this study,a microelectrode array(MEA)based on the shape and anatomical location of the BLA in the brain was designed and manufactured.Rats were injected with lipopolysaccharide(LPS)for 7 consecutive days to induce depressive behavior.We used the MEA to detect neural activity in the BLA before modeling,during modeling,and after LPS administration on 7 consecutive days.The results showed that after LPS treatment,the spike firing of neurons in the BLA region of rats gradually became more intense,and the local field potential power also increased progressively.Further analysis revealed that after LPS administration,the spike firing of BLA neurons was predominantly in the theta rhythm,with obvious periodic firing characteristics appearing after the 7 d of LPS administration,and the relative power of the local field potential in the theta band also significantly increased.In summary,our results suggest that the enhanced activity of BLA neurons in the theta band is related to the depressive state of rats,providing valuable guidance for research into the neural mechanisms of depression.展开更多
基金sponsored by the National Natural Science Foundation of China(Nos.61960206012,T2293730,T2293731,62121003,62171434,62333020,62374004,81971348,and 61673024)the National Key Research and Development Program of China(Nos.2022YFB3205602 and 2022YFC2402501)Major Program of Scientific and Technical Innovation 2030(No.2021ZD02016030).
文摘Depression is a common and severely debilitating neuropsychiatric disorder.Multiple studies indicate a strong correlation between the occurrence of immunological inflammation and the presence of depression.The basolateral amygdala(BLA)is crucial in the cognitive and physiological processing and control of emotion.However,due to the lack of detection tools,the neural activity of the BLA during depression is not well understood.In this study,a microelectrode array(MEA)based on the shape and anatomical location of the BLA in the brain was designed and manufactured.Rats were injected with lipopolysaccharide(LPS)for 7 consecutive days to induce depressive behavior.We used the MEA to detect neural activity in the BLA before modeling,during modeling,and after LPS administration on 7 consecutive days.The results showed that after LPS treatment,the spike firing of neurons in the BLA region of rats gradually became more intense,and the local field potential power also increased progressively.Further analysis revealed that after LPS administration,the spike firing of BLA neurons was predominantly in the theta rhythm,with obvious periodic firing characteristics appearing after the 7 d of LPS administration,and the relative power of the local field potential in the theta band also significantly increased.In summary,our results suggest that the enhanced activity of BLA neurons in the theta band is related to the depressive state of rats,providing valuable guidance for research into the neural mechanisms of depression.
