OBJECTIVE:To investigate the antimicrobial activity of commercially available honey and raw honey samples in Khyber Pakhtunkhwa,Pakistan,against pathogenic bacterial strains.METHODS:Well diffusion assays were performe...OBJECTIVE:To investigate the antimicrobial activity of commercially available honey and raw honey samples in Khyber Pakhtunkhwa,Pakistan,against pathogenic bacterial strains.METHODS:Well diffusion assays were performed to screen pure and diluted honey samples for antibacterial activity against six Gram-negative and six Gram-positive bacterial strains.Zones of inhibition were measured and compared with 10 mg Gentamycin.RESULTS:When honey samples were diluted to20%-70%,the honey samples showed no activity to mild antibacterial activity.The highest antibacterial activity was recorded when 90%and pure undiluted honey samples were tested and compared with a control Gentamycin disc(10 mg).CONCLUSION:Commercially processed honey and raw honey samples from north-west of Pakistan possess good antimicrobial potential.展开更多
Magnesium(Mg)stands out in temporary biomaterial applications due to its biocompatibility,biodegradability,and low Young’s modulus.However,controlling its corrosion through next-generation polymer-based functional co...Magnesium(Mg)stands out in temporary biomaterial applications due to its biocompatibility,biodegradability,and low Young’s modulus.However,controlling its corrosion through next-generation polymer-based functional coatings is crucial due to the rapid degradation behavior of Mg.In this study,the function of 2D lamellar Ti_(3)C_(2)T_(x)(MXene)in Hydroxyapatite(HA)and Halloysite nanotube(HNT)hybrid coatings in biodegradable poly-(lactic acid)(PLA)was investigated.The morphological and structural characterizations of the coatings on Mg were revealed through HRTEM,XPS,SEM-EDX,XRD,FTIR,and contact angle analyses/tests.Electrochemical in vitro corrosion tests(OCP,PDS,and EIS-Nyquist)were conducted for evaluate corrosion resistance under simulated body fluid(SBF)conditions.The bioactivity of the coatings in SBF have been revealed in accordance with the ISO 23,317 standard.Finally,antibacterial disk diffusion tests were conducted to investigate the functional effect of MXene in coatings.It has been determined that the presence of MXene in the coating increased not only surface wettability(131°,85°,77°,and 74°for uncoated,pH,PHH,and PHH/MXene coatings,respectively)but also increased corrosion resistance(1857.850,42.357,1.593,and 0.085×10^(-6),A/cm^(2) for uncoated,pH,PHH,and PHH/MXene coatings,respectively).It has been proven that the in vitro bioactivity of PLA-HA coatings is further enhanced by adding HNT and MXene,along with SEM morphologies after SBF.Finally,2D lamellar MXene-filled coating exhibits antibacterial behavior against both E.coli and S.aureus bacteria.展开更多
Green nanotechnology involves the synthesis of nanoparticles without the involvement of toxic chemicals. The present study reports the green synthesis of silver nanoparticles from Annona squamosa leaf extract rapidly ...Green nanotechnology involves the synthesis of nanoparticles without the involvement of toxic chemicals. The present study reports the green synthesis of silver nanoparticles from Annona squamosa leaf extract rapidly within 20 min. High throughput characterization such as UV-vis spectroscopy, Fourier Transform-Infrared Spectroscopy (FT-IR), Transmission Electron Microscopy (TEM), Field Emission-Scanning Electron Microscopy (FE-SEM), X-ray diffraction (XRD) and Zeta potential measurements disclose the existence of silver nanoparticles. The phenolic compounds present in the aqueous leaves extract paves way for the possible reduction of silver to nano-silver. Electron microscopic studies revealed the average size of nanoparticles were 52 nm. The nanoparticles are highly stable by reaching a zeta potential value of −33.6 mV. Comparative antibacterial efficacy of silver nanoparticles was investigated by disk diffusion and microtitre broth dilution method against Escherichia coli. The result showed that silver nanoparticles are toxic to E. coli cells at higher concentration. Overall, we suggest microtitre broth dilution method is more reliable to determine the antimicrobial activity of silver nanoparticles than disk diffusion method.展开更多
Objective:To assess ethanolic extract of the leaves of Melia azedarach L.(Family-Meliaceae)for its possible antioxidant,analgesic and antibacterial activities in association with performing phytochemical evaluation.Me...Objective:To assess ethanolic extract of the leaves of Melia azedarach L.(Family-Meliaceae)for its possible antioxidant,analgesic and antibacterial activities in association with performing phytochemical evaluation.Methods:A number of phytochemical tests of this extract were utilized to distinguish the existence of different sorts of compounds.Qualitative and quantitative antioxidant activities were assessed by TLC and DPPH scavenging assay respectively.Acetic acid induced writhing test in mice and disk diffusion assay of the leaves extract were carried out to demonstrate the analgesic and antibacterial activities respectively.Results:The phytochemical assessment revealed the existence of alkaloids,flavonoids,glycosides,saponins and tannins like compounds.Ethanolic extract of the leaves demonstrated antioxidant,analgesic and antibacterial activities in vitro.Conclusion:Considering the study,this could justify the leaves extract’s bioactivities but,to substantiate the activity of individual compound further investigation is necessary.展开更多
文摘OBJECTIVE:To investigate the antimicrobial activity of commercially available honey and raw honey samples in Khyber Pakhtunkhwa,Pakistan,against pathogenic bacterial strains.METHODS:Well diffusion assays were performed to screen pure and diluted honey samples for antibacterial activity against six Gram-negative and six Gram-positive bacterial strains.Zones of inhibition were measured and compared with 10 mg Gentamycin.RESULTS:When honey samples were diluted to20%-70%,the honey samples showed no activity to mild antibacterial activity.The highest antibacterial activity was recorded when 90%and pure undiluted honey samples were tested and compared with a control Gentamycin disc(10 mg).CONCLUSION:Commercially processed honey and raw honey samples from north-west of Pakistan possess good antimicrobial potential.
文摘Magnesium(Mg)stands out in temporary biomaterial applications due to its biocompatibility,biodegradability,and low Young’s modulus.However,controlling its corrosion through next-generation polymer-based functional coatings is crucial due to the rapid degradation behavior of Mg.In this study,the function of 2D lamellar Ti_(3)C_(2)T_(x)(MXene)in Hydroxyapatite(HA)and Halloysite nanotube(HNT)hybrid coatings in biodegradable poly-(lactic acid)(PLA)was investigated.The morphological and structural characterizations of the coatings on Mg were revealed through HRTEM,XPS,SEM-EDX,XRD,FTIR,and contact angle analyses/tests.Electrochemical in vitro corrosion tests(OCP,PDS,and EIS-Nyquist)were conducted for evaluate corrosion resistance under simulated body fluid(SBF)conditions.The bioactivity of the coatings in SBF have been revealed in accordance with the ISO 23,317 standard.Finally,antibacterial disk diffusion tests were conducted to investigate the functional effect of MXene in coatings.It has been determined that the presence of MXene in the coating increased not only surface wettability(131°,85°,77°,and 74°for uncoated,pH,PHH,and PHH/MXene coatings,respectively)but also increased corrosion resistance(1857.850,42.357,1.593,and 0.085×10^(-6),A/cm^(2) for uncoated,pH,PHH,and PHH/MXene coatings,respectively).It has been proven that the in vitro bioactivity of PLA-HA coatings is further enhanced by adding HNT and MXene,along with SEM morphologies after SBF.Finally,2D lamellar MXene-filled coating exhibits antibacterial behavior against both E.coli and S.aureus bacteria.
文摘Green nanotechnology involves the synthesis of nanoparticles without the involvement of toxic chemicals. The present study reports the green synthesis of silver nanoparticles from Annona squamosa leaf extract rapidly within 20 min. High throughput characterization such as UV-vis spectroscopy, Fourier Transform-Infrared Spectroscopy (FT-IR), Transmission Electron Microscopy (TEM), Field Emission-Scanning Electron Microscopy (FE-SEM), X-ray diffraction (XRD) and Zeta potential measurements disclose the existence of silver nanoparticles. The phenolic compounds present in the aqueous leaves extract paves way for the possible reduction of silver to nano-silver. Electron microscopic studies revealed the average size of nanoparticles were 52 nm. The nanoparticles are highly stable by reaching a zeta potential value of −33.6 mV. Comparative antibacterial efficacy of silver nanoparticles was investigated by disk diffusion and microtitre broth dilution method against Escherichia coli. The result showed that silver nanoparticles are toxic to E. coli cells at higher concentration. Overall, we suggest microtitre broth dilution method is more reliable to determine the antimicrobial activity of silver nanoparticles than disk diffusion method.
基金the authorities of Pharmacy Discipline,Life Science School,Khulna University for supporting fund(Grant No.KU:PHRM:2011/April-071112).
文摘Objective:To assess ethanolic extract of the leaves of Melia azedarach L.(Family-Meliaceae)for its possible antioxidant,analgesic and antibacterial activities in association with performing phytochemical evaluation.Methods:A number of phytochemical tests of this extract were utilized to distinguish the existence of different sorts of compounds.Qualitative and quantitative antioxidant activities were assessed by TLC and DPPH scavenging assay respectively.Acetic acid induced writhing test in mice and disk diffusion assay of the leaves extract were carried out to demonstrate the analgesic and antibacterial activities respectively.Results:The phytochemical assessment revealed the existence of alkaloids,flavonoids,glycosides,saponins and tannins like compounds.Ethanolic extract of the leaves demonstrated antioxidant,analgesic and antibacterial activities in vitro.Conclusion:Considering the study,this could justify the leaves extract’s bioactivities but,to substantiate the activity of individual compound further investigation is necessary.
