AIM:To investigate the efficacy and mechanisms of indirect intense pulsed light(IPL)irradiation on meibomian gland dysfunction(MGD).METHODS:A total of 60 MGD patients was included in this prospective randomized contro...AIM:To investigate the efficacy and mechanisms of indirect intense pulsed light(IPL)irradiation on meibomian gland dysfunction(MGD).METHODS:A total of 60 MGD patients was included in this prospective randomized controlled trial.Patients were randomly assigned 1:1 into two groups(3-mm group and 10-mm group)in which IPL was applied at distances from the lower eyelid margin of 3 and 10 mm,respectively.Both groups received three times treatment with 3-week interval.Meibomian gland yield secretion score(MGYSS),standard patient evaluation of eye dryness(SPEED)questionnaire,tear break-up time(TBUT),corneal fluorescein staining(CFS),and in vivo confocal microscopy were performed at baseline and after every treatment.RESULTS:After three IPL treatments,both groups had significant improvement in MGYSS(both P<0.05).The noninferiority test showed that improvement in 10-mm group was not inferior to that in 3-mm group(P<0.001).In both groups,temporal regions of both upper and lower eyelids showed significant improvement in MGYSS.Scores of SPEED questionnaire in both groups declined significantly(both P<0.001)and changes of SPEED had no difference between two groups(P=0.57).Density of central corneal subepithelial nerves and TBUTs showed no statistically significant changes.The 3-mm group had improvement on corneal fluorescein staining(P=0.048)and meibomian gland morphology(acini wall thickness P=0.003,hyperreflective points P=0.024)while the 10-mm group had not.CONCLUSION:The efficacy of IPL indirect irradiation in improving meibomian gland secretion and alleviating dry eye symptoms remains unchanged with increase in treatment distance.IPL may primarily act on the functional improvement of the meibomian glands and corneal nerves.展开更多
Dengue fever(DF) and dengue haemorrhagic fever(DHF) are important mosquito-borne viral diseases of humans and recognized as important emerging infectious diseases in the tropics and subtopics.Compared to nine reportin...Dengue fever(DF) and dengue haemorrhagic fever(DHF) are important mosquito-borne viral diseases of humans and recognized as important emerging infectious diseases in the tropics and subtopics.Compared to nine reporting countries in the 1950s,today the geographic distribution includes more than 100 countries worldwide.Dengue viral infections are known to present a diverse clinical spectrum,ranging from asymptomatic illness to fatal dengue shock syndrome. Mild hepatic dysfunction in dengue haemorrhagic fever is usual.However,its presentation as acute liver failure(ALF) is unusual.We report a patient with dengue shock syndrome who presented with acute liver failure and hepatic encephalopathy in a recent outbreak of dengue fever in Delhi,India.展开更多
We present the case of an 86-year-old woman referred to us from Orthopaedics Division after surgical treatment for femur fracture without personal or family history of ischemic cardiopathy. During hospitalization the ...We present the case of an 86-year-old woman referred to us from Orthopaedics Division after surgical treatment for femur fracture without personal or family history of ischemic cardiopathy. During hospitalization the patient had stomach-ache and the electrocardiogram demonstrated ST-segment elevation in anterior leads. The coronarography showed haziness, but no critical stenosis;ventricolography revealed apical ballooning of the left ventricle with severe systolic dysfunction. She was treated with dopamine in renal dose, beta-blockers and warfarin. When she was in fairly good condition, she started physiotherapy for endoprosthesis for the femur fracture. Clinicians should consider takotsubo cardiomyopathy in the differential diagnosis of patients presenting with chest pain, especially in women with a recent history of physical stress.展开更多
Backround: Erectile dysfunction (ED) is the inability of male reproductive organs within sexual intercourse caused by neurogenic and hormonal disorders. There are some causes of ED such as hypertension, stress, neurol...Backround: Erectile dysfunction (ED) is the inability of male reproductive organs within sexual intercourse caused by neurogenic and hormonal disorders. There are some causes of ED such as hypertension, stress, neurological disorders, stroke, diabetes, atherosclerosis, lifestyle, alcohol, smoking, and age-related hormonal decline can cause infertility. The natural treatment of sexual dysfunction through aphrodisiac activity of the plant is to increase sexual hormones, spermatogenesis activity and through PDE5 inhibitors such as sildenafil, verdenafil, and tadalafil which can inhibit the hydrolysis of second messenger cGMP of penis smooth muscle cells. Primer Design for amplification of several PDE5 gene nucleotide sequences obtained from NCBI gen banks and tested directly through explosions at NCBI and also using MEGA 6, primer 3 plus, and fast PCR software. The natural treatment of sexual dysfunction through aphrodisiac activity from plants to increase sexual hormone, spermatogenesis activity and inhibitor PDE5 such as sildenafil, vardenafil, and tadalafil can inhibit hydrolysis second messenger of cells cGMP smooth penis muscles. Method: Primer design stages for several sequences are data supply, multiple sequence alignment, sequence trimming, primer design (fastPCR input), in silico PCR analysis, and primer evaluation (Primer Test, OligoCalc and BLAST). Results: Primer of PDE5 that is choosen is with reverse sequence 5-TGCATTGACCATGTCTCTCGTT-3, forward 5-CGCCGATCTGGGCTGAACTA-3 able to amplify template DNA at temperature 67.2°C, 65.8°C, 63.7°C, however, the DNA band fragment looks not very clear, while it is more clearly seen at Tm temperature 61.2°C, 59.1°C, 57.8°C and 57°C. Conclusion: PDE5 primers can be amplified well at temperature 61.2°C, 59.1°C, 57.8°C and 57°C. PDE5 primer succeeded in amplifying DNA with a product length of 402 bp.展开更多
Polycystic ovary syndrome(PCOS)is the predominant cause of subfertility in reproductive-aged women;however,its pathophysiology remains unknown.Neurotensin(NTS)is a member of the gut–brain peptide family and is involv...Polycystic ovary syndrome(PCOS)is the predominant cause of subfertility in reproductive-aged women;however,its pathophysiology remains unknown.Neurotensin(NTS)is a member of the gut–brain peptide family and is involved in ovulation;its relationship with PCOS is unclear.Here,we found that NTS expression in ovarian granulosa cells and follicular fluids was markedly decreased in patients with PCOS.In the in vitro culture of cumulus–oocyte complexes,the neurotensin receptor 1(NTSR1)antagonist SR48692 blocked cumulus expansion and oocyte meiotic maturation by inhibiting metabolic cooperation and damaging the mitochondrial structure in oocytes and surrounding cumulus cells.Furthermore,the ERK1/2-early growth response 1 pathway was found to be a key downstream mediator of NTS/NTSR1 in the ovulatory process.Animal studies showed that in vivo injection of SR48692 in mice reduced ovulation efficiency and contributed to irregular estrus cycles and polycystic ovary morphology.By contrast,NTS partially ameliorated the ovarian abnormalities in mice with dehydroepiandrosterone-induced PCOS.Our findings highlighted the critical role of NTS reduction and consequent abnormal NTSR1 signaling in the ovulatory dysfunction of PCOS,suggesting a potential strategy for PCOS treatment.展开更多
The dysfunction of the lysosome and autophagy-lysosome system serves as a driving force for neurodegenerative diseases,metabolic disorders,inflammatory conditions,and other related diseases,closely influencing their o...The dysfunction of the lysosome and autophagy-lysosome system serves as a driving force for neurodegenerative diseases,metabolic disorders,inflammatory conditions,and other related diseases,closely influencing their onset and progression.Therefore,restoring the function of the lysosome or autophagy-lysosome system has become an increasingly crucial therapeutic strategy in disease management.In this review,we will introduce the lysosomal biogenesis,structure,and function,as well as the biological process of the autophagy-lysosome system.Various diseases closely associated with lysosomal/autophagic dysfunction are also reviewed,emphasizing the significance of targeting the function of the lysosome or autophagy-lysosome system in disease treatment.Finally,we focus on engineered nanomaterials that have the capabilities to restore the function of the lysosome or autophagy-lysosome system,and summarize different strategies and methods for achieving this goal.This review aims to elucidate the latest progress in the field of nanomedicine for lysosomal/autophagic defect-related diseases and inspire the development of innovative and clinically valuable nanomedicines.展开更多
基金Supported by the National Natural Science Foundation of China(No.81870629).
文摘AIM:To investigate the efficacy and mechanisms of indirect intense pulsed light(IPL)irradiation on meibomian gland dysfunction(MGD).METHODS:A total of 60 MGD patients was included in this prospective randomized controlled trial.Patients were randomly assigned 1:1 into two groups(3-mm group and 10-mm group)in which IPL was applied at distances from the lower eyelid margin of 3 and 10 mm,respectively.Both groups received three times treatment with 3-week interval.Meibomian gland yield secretion score(MGYSS),standard patient evaluation of eye dryness(SPEED)questionnaire,tear break-up time(TBUT),corneal fluorescein staining(CFS),and in vivo confocal microscopy were performed at baseline and after every treatment.RESULTS:After three IPL treatments,both groups had significant improvement in MGYSS(both P<0.05).The noninferiority test showed that improvement in 10-mm group was not inferior to that in 3-mm group(P<0.001).In both groups,temporal regions of both upper and lower eyelids showed significant improvement in MGYSS.Scores of SPEED questionnaire in both groups declined significantly(both P<0.001)and changes of SPEED had no difference between two groups(P=0.57).Density of central corneal subepithelial nerves and TBUTs showed no statistically significant changes.The 3-mm group had improvement on corneal fluorescein staining(P=0.048)and meibomian gland morphology(acini wall thickness P=0.003,hyperreflective points P=0.024)while the 10-mm group had not.CONCLUSION:The efficacy of IPL indirect irradiation in improving meibomian gland secretion and alleviating dry eye symptoms remains unchanged with increase in treatment distance.IPL may primarily act on the functional improvement of the meibomian glands and corneal nerves.
文摘Dengue fever(DF) and dengue haemorrhagic fever(DHF) are important mosquito-borne viral diseases of humans and recognized as important emerging infectious diseases in the tropics and subtopics.Compared to nine reporting countries in the 1950s,today the geographic distribution includes more than 100 countries worldwide.Dengue viral infections are known to present a diverse clinical spectrum,ranging from asymptomatic illness to fatal dengue shock syndrome. Mild hepatic dysfunction in dengue haemorrhagic fever is usual.However,its presentation as acute liver failure(ALF) is unusual.We report a patient with dengue shock syndrome who presented with acute liver failure and hepatic encephalopathy in a recent outbreak of dengue fever in Delhi,India.
文摘We present the case of an 86-year-old woman referred to us from Orthopaedics Division after surgical treatment for femur fracture without personal or family history of ischemic cardiopathy. During hospitalization the patient had stomach-ache and the electrocardiogram demonstrated ST-segment elevation in anterior leads. The coronarography showed haziness, but no critical stenosis;ventricolography revealed apical ballooning of the left ventricle with severe systolic dysfunction. She was treated with dopamine in renal dose, beta-blockers and warfarin. When she was in fairly good condition, she started physiotherapy for endoprosthesis for the femur fracture. Clinicians should consider takotsubo cardiomyopathy in the differential diagnosis of patients presenting with chest pain, especially in women with a recent history of physical stress.
文摘Backround: Erectile dysfunction (ED) is the inability of male reproductive organs within sexual intercourse caused by neurogenic and hormonal disorders. There are some causes of ED such as hypertension, stress, neurological disorders, stroke, diabetes, atherosclerosis, lifestyle, alcohol, smoking, and age-related hormonal decline can cause infertility. The natural treatment of sexual dysfunction through aphrodisiac activity of the plant is to increase sexual hormones, spermatogenesis activity and through PDE5 inhibitors such as sildenafil, verdenafil, and tadalafil which can inhibit the hydrolysis of second messenger cGMP of penis smooth muscle cells. Primer Design for amplification of several PDE5 gene nucleotide sequences obtained from NCBI gen banks and tested directly through explosions at NCBI and also using MEGA 6, primer 3 plus, and fast PCR software. The natural treatment of sexual dysfunction through aphrodisiac activity from plants to increase sexual hormone, spermatogenesis activity and inhibitor PDE5 such as sildenafil, vardenafil, and tadalafil can inhibit hydrolysis second messenger of cells cGMP smooth penis muscles. Method: Primer design stages for several sequences are data supply, multiple sequence alignment, sequence trimming, primer design (fastPCR input), in silico PCR analysis, and primer evaluation (Primer Test, OligoCalc and BLAST). Results: Primer of PDE5 that is choosen is with reverse sequence 5-TGCATTGACCATGTCTCTCGTT-3, forward 5-CGCCGATCTGGGCTGAACTA-3 able to amplify template DNA at temperature 67.2°C, 65.8°C, 63.7°C, however, the DNA band fragment looks not very clear, while it is more clearly seen at Tm temperature 61.2°C, 59.1°C, 57.8°C and 57°C. Conclusion: PDE5 primers can be amplified well at temperature 61.2°C, 59.1°C, 57.8°C and 57°C. PDE5 primer succeeded in amplifying DNA with a product length of 402 bp.
基金supported by grants from the National Key Research and Development Program of China(Nos.2022YFC2703204 and 2021YFC2701104)the National Natural Science Foundation of China(Nos.81971343 and 82171623)+3 种基金the National Institutes of Health Project(No.1R01HD085527),the Innovative research team of high-level local universities in Shanghai(No.SHSMU-ZLCX20210200)Shanghai Commission of Science and Technology(Nos.21XD1401900 and 20DZ2270900)Three-Year Action Plan for Strengthening the Construction of the Public Health System in Shanghai(No.GWVI-11.1-36)Shanghai’s Top Priority Research Center Construction Project(No.2023ZZ02002).
文摘Polycystic ovary syndrome(PCOS)is the predominant cause of subfertility in reproductive-aged women;however,its pathophysiology remains unknown.Neurotensin(NTS)is a member of the gut–brain peptide family and is involved in ovulation;its relationship with PCOS is unclear.Here,we found that NTS expression in ovarian granulosa cells and follicular fluids was markedly decreased in patients with PCOS.In the in vitro culture of cumulus–oocyte complexes,the neurotensin receptor 1(NTSR1)antagonist SR48692 blocked cumulus expansion and oocyte meiotic maturation by inhibiting metabolic cooperation and damaging the mitochondrial structure in oocytes and surrounding cumulus cells.Furthermore,the ERK1/2-early growth response 1 pathway was found to be a key downstream mediator of NTS/NTSR1 in the ovulatory process.Animal studies showed that in vivo injection of SR48692 in mice reduced ovulation efficiency and contributed to irregular estrus cycles and polycystic ovary morphology.By contrast,NTS partially ameliorated the ovarian abnormalities in mice with dehydroepiandrosterone-induced PCOS.Our findings highlighted the critical role of NTS reduction and consequent abnormal NTSR1 signaling in the ovulatory dysfunction of PCOS,suggesting a potential strategy for PCOS treatment.
基金supported by the Taishan Scholars Program of Shandong Province(No.tsqn202103112)the Shandong Provincial Natural Science Foundation(No.ZR2021QB202)+2 种基金the Development Plan of Youth Innovation Team in Colleges and Universities of Shandong Province(No.2021KJ052)the Shandong-Chongqing Science and Technology Cooperation Project for Technological Innovation and Application Development(No.CSTB2023TIAD-LDX0015)the Supporting Fund for Leading Talents above Provincial Level in Yantai,China.
文摘The dysfunction of the lysosome and autophagy-lysosome system serves as a driving force for neurodegenerative diseases,metabolic disorders,inflammatory conditions,and other related diseases,closely influencing their onset and progression.Therefore,restoring the function of the lysosome or autophagy-lysosome system has become an increasingly crucial therapeutic strategy in disease management.In this review,we will introduce the lysosomal biogenesis,structure,and function,as well as the biological process of the autophagy-lysosome system.Various diseases closely associated with lysosomal/autophagic dysfunction are also reviewed,emphasizing the significance of targeting the function of the lysosome or autophagy-lysosome system in disease treatment.Finally,we focus on engineered nanomaterials that have the capabilities to restore the function of the lysosome or autophagy-lysosome system,and summarize different strategies and methods for achieving this goal.This review aims to elucidate the latest progress in the field of nanomedicine for lysosomal/autophagic defect-related diseases and inspire the development of innovative and clinically valuable nanomedicines.
