AIM: To investigate the diagnostic utility of beta 2 microglobulin (B2-M) levels and analyze this correlation with the activity of inflammatory bowel disease (IBD).
The possibility of an infectious origin in inflammatory bowel disease(IBD)has been postulated since the first description of Crohn’s disease(CD).Many observations implicate bacteria as a trigger for the development o...The possibility of an infectious origin in inflammatory bowel disease(IBD)has been postulated since the first description of Crohn’s disease(CD).Many observations implicate bacteria as a trigger for the development of CD:lesions occur in regions with higher bacterial concentrations;aphthous ulcers occur in Peyer’s patches;inflammation resolves when the fecal stream is diverted and is reactivated following reinfusion ofbowel contents;severity of the disease is correlated with bacterial density in the mucosa;granulomas can contain bacteria;and susceptible mice raised in germfree conditions develop inflammation when bacteria are introduced in the 1990’s,several studies sought to establish a relationship with viral infections and the onset of IBD,finally concluding that no direct link had been demonstrated.In the past fifteen years,evidence relating IBD pathogenesis to Mycobacterium avium paratuberculosis,salmonella,campylobacter,etc.,has been found.The tendency now under discussion to regard microbiota as the primary catalyst has led to the latest studies on microbiota as pathogens,focusing on Escherichia coli,mainly in ileal CD.The present review discusses the literature available on these"bugs".展开更多
Oxidative stress is caused by an imbalance between the production of reactive oxygen(free radicals) and the body's ability(antioxidant capacity) to readily detoxify the reactive intermediates or easily repair the ...Oxidative stress is caused by an imbalance between the production of reactive oxygen(free radicals) and the body's ability(antioxidant capacity) to readily detoxify the reactive intermediates or easily repair the resulting damage. An adequate diet, characterized by daily intake of foods associated with improvementsin the total antioxidant capacity of individuals and reduced incidence of diseases related to oxidation, can modulate the degree of oxidative stress. In fact, dietderived micronutrients may be direct antioxidants, or are components of antioxidant enzymes, leading to improvement of some indicators of hepatic function. However, although their increased dietary intake might be beneficial, literature data are still controversial. This review summarizes what is known about the effects of diet nutrients on oxidative stress, inflammation and liver function. Moreover, we have analyzed:(1) the main nutritional components involved in the production and/or removal of free radicals; and(2) the role of free radicals in the pathogenesis of several hepatic diseases and related comorbidities.展开更多
There are several reports of anti-tumor necrosis factor(TNF)-induced lung disease,especially in patients with rheumatologic diseases.Adalimumab is an antiTNF drug used to induce and maintain remission in patients with...There are several reports of anti-tumor necrosis factor(TNF)-induced lung disease,especially in patients with rheumatologic diseases.Adalimumab is an antiTNF drug used to induce and maintain remission in patients with immune-mediated diseases,such as Crohn’s disease.Although pulmonary disorders could be an extra-intestinal manifestation of inflammatory bowel disease,biologic therapy could also be a cause of lung injury.Only few cases of adalimumab-induced lung toxicity have been reported,and the majority of them were in patients with rheumatologic diseases.Lung injury secondary to anti-TNF therapy should,after ruling out other etiologies,be considered in patients who have a temporal association between the onset of respiratory symptoms and the exposure to these drugs.A compatible pattern in the biopsy and the clinical improvement after discontinuation of the anti-TNF drug would strongly support the diagnosis.展开更多
AIM: To analyze the cellular immune response towards microsatellite-instability(MSI)-induced frameshift-peptides(FSPs) in patients suffering from inflammatory bowel disease(IBD) with and without thiopurine-based immun...AIM: To analyze the cellular immune response towards microsatellite-instability(MSI)-induced frameshift-peptides(FSPs) in patients suffering from inflammatory bowel disease(IBD) with and without thiopurine-based immunosuppressive treatment.METHODS: Frequencies of peripheral blood T cell responses of IBD patients(n = 75) against FSPs derived from 14 microsatellite-containing candidate genes were quantified by interferon-γ enzyme-linked immunospot.T cells derived from 20 healthy individuals served as controls.RESULTS: Significant T cell reactivities against MSIinduced FSPs were observed in 59 of 75 IBD patients(78.7%).This was significantly more as we could observe in 20 healthy controls(P = 0.001).Overall,the reactivity was significantly influenced by thiopurine treatment(P = 0.032) and duration of disease(P = 0.002) but not by duration or cumulative amount of thiopurine therapy(P = 0.476).Unexpected,15 of 24(62.5%) IBD patients without prior thiopurine treatment also showed increased FSP-specific immune responses(P = 0.001).CONCLUSION: These findings propose FSPs as potential novel class of inflammation-associated antigens and this in turn may have implications for screening,diagnosis as well as clinical management of patients suffering from IBD and other inflammatory conditions.展开更多
DISE learning model refers to the one which is dialogue-based using scientific approach containing edutainment element. DISE is the acronym of dialog, scientific, and edutainment. It is a learning model which is devel...DISE learning model refers to the one which is dialogue-based using scientific approach containing edutainment element. DISE is the acronym of dialog, scientific, and edutainment. It is a learning model which is developed for the fifth graders of elementary school particularly for teaching writing of personal recount. This article described DISE learning model from the theoretical basis, meaning, and syntax. DISE was the product of Research and Development design conducted in Banyumas Regency, Central Java Province, Indonesia. DISE learning model can serve as a reference for elementary school teachers for teaching writing.展开更多
DUBBED a“paradise on earth”for its picturesque West Lake,Hangzhou in east China’s Zhejiang Province is also known as a business center and culinary hub.Historically,it was the capital of the Southern Song Dynasty(1...DUBBED a“paradise on earth”for its picturesque West Lake,Hangzhou in east China’s Zhejiang Province is also known as a business center and culinary hub.Historically,it was the capital of the Southern Song Dynasty(1127-1279),when numerous literati residing there helped create a unique charm of the Hangzhou cuisine featuring the combination of culinary essence from north and south China.展开更多
To study the role of interleukin(IL)-10 in acute-graft-versus-host disease (aGVHD) and graft rejection Methods Serum concentrations of IL-10 in 28 patients who underwent allogeneic hematopoietic stem cell transpla...To study the role of interleukin(IL)-10 in acute-graft-versus-host disease (aGVHD) and graft rejection Methods Serum concentrations of IL-10 in 28 patients who underwent allogeneic hematopoietic stem cell transplantation (allo-HSCT) were measured by enzymed-linked immunosorbent assay (ELISA) IL-10 gene expression in peripheral mononuclear cells was measured by reverse-transcriptase polymerase chain reaction (RT-PCR) after transplantation Results Seven patients developed grade Ⅰ GVHD, 7 patients developed grade Ⅱ-Ⅳ GVHD, 4 patients had graft rejection Before transplantation, the concentrations of IL-10 were higher in patients who later did not developed aGVHD After transplantation, IL-10 levels increased in patients without aGVHD, but decreased in patients with aGVHD or graft rejection And IL-10mRNA was more frequent in patients without aGVHD compared to those with aGVHD Conclusions IL-10 plays a negative role in the development of aGVHD and graft rejection展开更多
Living on a tropical island,with waves lappingup on thesand and the sun shining-sounds like a dream come true,right?Well,for me,it was a 100-percent no.After seven years in China and always wondering what it would be ...Living on a tropical island,with waves lappingup on thesand and the sun shining-sounds like a dream come true,right?Well,for me,it was a 100-percent no.After seven years in China and always wondering what it would be like to live in paradise,I decided to take the plunge and move to another country,destination Thailand.展开更多
AIM: To examine the effect of farnesoid X receptor (FXR) activation by GW4064 on endotoxin-induced hepatic inflammation in nonalcoholic fatty liver disease (NAFLD) and the underlying mechanism.
科学研究在经历了实验科学、理论科学、计算科学阶段后,进入了数据密集型科学阶段,与之相伴的是大数据时代的到来.大数据泛指规模达到几百TB,甚至PB级的数据①,其典型的特征是分布、异构、低质量等.尽管传统数据库管理技术(特别是商业...科学研究在经历了实验科学、理论科学、计算科学阶段后,进入了数据密集型科学阶段,与之相伴的是大数据时代的到来.大数据泛指规模达到几百TB,甚至PB级的数据①,其典型的特征是分布、异构、低质量等.尽管传统数据库管理技术(特别是商业关系型数据库)在过去40年间取得了巨大成功,但是这些技术和系统无法有效管理支持数据密集型科学与工程(Data-Intensive Science and Engineering,DISE)的大数据.文中探讨数据密集型科学与工程的具体需求和现实挑战.它涵盖的内容表现在4个层面,包括数据存储与组织、计算方法、数据分析以及用户接口技术等.同时,数据质量、数据安全、数据监护等内容也需要在各层面得到重视.文中尝试梳理了数据密集型科学与工程的整体架构,回顾了相关领域的新近发展,分析了面临的挑战,探讨了未来的研究方向.展开更多
文摘AIM: To investigate the diagnostic utility of beta 2 microglobulin (B2-M) levels and analyze this correlation with the activity of inflammatory bowel disease (IBD).
文摘The possibility of an infectious origin in inflammatory bowel disease(IBD)has been postulated since the first description of Crohn’s disease(CD).Many observations implicate bacteria as a trigger for the development of CD:lesions occur in regions with higher bacterial concentrations;aphthous ulcers occur in Peyer’s patches;inflammation resolves when the fecal stream is diverted and is reactivated following reinfusion ofbowel contents;severity of the disease is correlated with bacterial density in the mucosa;granulomas can contain bacteria;and susceptible mice raised in germfree conditions develop inflammation when bacteria are introduced in the 1990’s,several studies sought to establish a relationship with viral infections and the onset of IBD,finally concluding that no direct link had been demonstrated.In the past fifteen years,evidence relating IBD pathogenesis to Mycobacterium avium paratuberculosis,salmonella,campylobacter,etc.,has been found.The tendency now under discussion to regard microbiota as the primary catalyst has led to the latest studies on microbiota as pathogens,focusing on Escherichia coli,mainly in ileal CD.The present review discusses the literature available on these"bugs".
文摘Oxidative stress is caused by an imbalance between the production of reactive oxygen(free radicals) and the body's ability(antioxidant capacity) to readily detoxify the reactive intermediates or easily repair the resulting damage. An adequate diet, characterized by daily intake of foods associated with improvementsin the total antioxidant capacity of individuals and reduced incidence of diseases related to oxidation, can modulate the degree of oxidative stress. In fact, dietderived micronutrients may be direct antioxidants, or are components of antioxidant enzymes, leading to improvement of some indicators of hepatic function. However, although their increased dietary intake might be beneficial, literature data are still controversial. This review summarizes what is known about the effects of diet nutrients on oxidative stress, inflammation and liver function. Moreover, we have analyzed:(1) the main nutritional components involved in the production and/or removal of free radicals; and(2) the role of free radicals in the pathogenesis of several hepatic diseases and related comorbidities.
基金Supported by CIBEREHD is founded by Instituto de Salud Carlos Ⅲ
文摘There are several reports of anti-tumor necrosis factor(TNF)-induced lung disease,especially in patients with rheumatologic diseases.Adalimumab is an antiTNF drug used to induce and maintain remission in patients with immune-mediated diseases,such as Crohn’s disease.Although pulmonary disorders could be an extra-intestinal manifestation of inflammatory bowel disease,biologic therapy could also be a cause of lung injury.Only few cases of adalimumab-induced lung toxicity have been reported,and the majority of them were in patients with rheumatologic diseases.Lung injury secondary to anti-TNF therapy should,after ruling out other etiologies,be considered in patients who have a temporal association between the onset of respiratory symptoms and the exposure to these drugs.A compatible pattern in the biopsy and the clinical improvement after discontinuation of the anti-TNF drug would strongly support the diagnosis.
基金Supported by University of Rostock(FORUN program)
文摘AIM: To analyze the cellular immune response towards microsatellite-instability(MSI)-induced frameshift-peptides(FSPs) in patients suffering from inflammatory bowel disease(IBD) with and without thiopurine-based immunosuppressive treatment.METHODS: Frequencies of peripheral blood T cell responses of IBD patients(n = 75) against FSPs derived from 14 microsatellite-containing candidate genes were quantified by interferon-γ enzyme-linked immunospot.T cells derived from 20 healthy individuals served as controls.RESULTS: Significant T cell reactivities against MSIinduced FSPs were observed in 59 of 75 IBD patients(78.7%).This was significantly more as we could observe in 20 healthy controls(P = 0.001).Overall,the reactivity was significantly influenced by thiopurine treatment(P = 0.032) and duration of disease(P = 0.002) but not by duration or cumulative amount of thiopurine therapy(P = 0.476).Unexpected,15 of 24(62.5%) IBD patients without prior thiopurine treatment also showed increased FSP-specific immune responses(P = 0.001).CONCLUSION: These findings propose FSPs as potential novel class of inflammation-associated antigens and this in turn may have implications for screening,diagnosis as well as clinical management of patients suffering from IBD and other inflammatory conditions.
文摘DISE learning model refers to the one which is dialogue-based using scientific approach containing edutainment element. DISE is the acronym of dialog, scientific, and edutainment. It is a learning model which is developed for the fifth graders of elementary school particularly for teaching writing of personal recount. This article described DISE learning model from the theoretical basis, meaning, and syntax. DISE was the product of Research and Development design conducted in Banyumas Regency, Central Java Province, Indonesia. DISE learning model can serve as a reference for elementary school teachers for teaching writing.
文摘DUBBED a“paradise on earth”for its picturesque West Lake,Hangzhou in east China’s Zhejiang Province is also known as a business center and culinary hub.Historically,it was the capital of the Southern Song Dynasty(1127-1279),when numerous literati residing there helped create a unique charm of the Hangzhou cuisine featuring the combination of culinary essence from north and south China.
基金ThisstudywassupportedbyagrantfromNationalNaturalSciencesFoundationofChina (No 39870 71 0 )
文摘To study the role of interleukin(IL)-10 in acute-graft-versus-host disease (aGVHD) and graft rejection Methods Serum concentrations of IL-10 in 28 patients who underwent allogeneic hematopoietic stem cell transplantation (allo-HSCT) were measured by enzymed-linked immunosorbent assay (ELISA) IL-10 gene expression in peripheral mononuclear cells was measured by reverse-transcriptase polymerase chain reaction (RT-PCR) after transplantation Results Seven patients developed grade Ⅰ GVHD, 7 patients developed grade Ⅱ-Ⅳ GVHD, 4 patients had graft rejection Before transplantation, the concentrations of IL-10 were higher in patients who later did not developed aGVHD After transplantation, IL-10 levels increased in patients without aGVHD, but decreased in patients with aGVHD or graft rejection And IL-10mRNA was more frequent in patients without aGVHD compared to those with aGVHD Conclusions IL-10 plays a negative role in the development of aGVHD and graft rejection
文摘Living on a tropical island,with waves lappingup on thesand and the sun shining-sounds like a dream come true,right?Well,for me,it was a 100-percent no.After seven years in China and always wondering what it would be like to live in paradise,I decided to take the plunge and move to another country,destination Thailand.
文摘AIM: To examine the effect of farnesoid X receptor (FXR) activation by GW4064 on endotoxin-induced hepatic inflammation in nonalcoholic fatty liver disease (NAFLD) and the underlying mechanism.
文摘科学研究在经历了实验科学、理论科学、计算科学阶段后,进入了数据密集型科学阶段,与之相伴的是大数据时代的到来.大数据泛指规模达到几百TB,甚至PB级的数据①,其典型的特征是分布、异构、低质量等.尽管传统数据库管理技术(特别是商业关系型数据库)在过去40年间取得了巨大成功,但是这些技术和系统无法有效管理支持数据密集型科学与工程(Data-Intensive Science and Engineering,DISE)的大数据.文中探讨数据密集型科学与工程的具体需求和现实挑战.它涵盖的内容表现在4个层面,包括数据存储与组织、计算方法、数据分析以及用户接口技术等.同时,数据质量、数据安全、数据监护等内容也需要在各层面得到重视.文中尝试梳理了数据密集型科学与工程的整体架构,回顾了相关领域的新近发展,分析了面临的挑战,探讨了未来的研究方向.
