To date, complex components of available reagents have been used for directional induction of neural stem cells into dopaminergic neurons, resulting in a poor ability to repeat experiments. This study sought to invest...To date, complex components of available reagents have been used for directional induction of neural stem cells into dopaminergic neurons, resulting in a poor ability to repeat experiments. This study sought to investigate whether a homogenate of the substantia nigra of adult rats and/or basic fibroblast growth factor could directionally induce neural stem cells derived from the subventricular zone of embryonic rats to differentiate into dopaminergic neurons. Tyrosine hydroxylase-positive cells were observed exclusively after induction with the homogenate supernatant of the substantia nigra from adult rats and basic fibroblast growth factor for 48 hours in vitro. However, in the groups treated with homogenate supernatant or basic fibroblast growth factor alone, tyrosine hydroxylase expression was not observed. Moreover, the content of dopamine in the culture medium of subventricular zone neurons was significantly increased at 48 hours after induction with the homogenate supernatant of the substantia nigra from adult rats and basic fibroblast growth factor. Experimental findings indicate that the homogenate supernatant of the substantia nigra from adult rats and basic fibroblast growth factor could directionally induce neural stem cells derived from the subventricular zone of embryonic rats to differentiate into dopaminergic neurons in the substantia nigra with the ability to secrete dopamine.展开更多
Event relation extraction plays a crucial role in constructing an event knowledge graph.However,current models only extract trigger words as event ontology representations,and do not consider node type during informat...Event relation extraction plays a crucial role in constructing an event knowledge graph.However,current models only extract trigger words as event ontology representations,and do not consider node type during information aggregation,resulting in low accuracy in event relation extraction.To address these challenges,we propose an event relation extraction model based on heterogeneous graph attention networks and event ontology direction induction.To enhance the completeness of event information,we incorporate argument role information,in addition to trigger words,into the input text.A novel heterogeneous graph attention framework is proposed to reasonably allocate weights to trigger words,argument roles,and text information,and then perform two levels of aggregation,node-level and semantic-level,in sequence.To improve the accuracy of event direction discrimination,we construct an event ontology subgraph that includes trigger words and arguments to aggregate complete event structure information during direction induction.Finally,we evaluate our model on three datasets,TimeBank-Dense,MATRES,and HiEve,and demonstrate that our model outperforms state-of-the-art models by 1.2%,0.5%,and 0.8%,respectively,in terms of the Micro-F1 score.Our proposed model provides a promising solution for event relation extraction and can be applied in various natural language processing applications.展开更多
基金sponsored by the General Program of Yunnan Provincial Application Basic Research, No. 2008ZC109M
文摘To date, complex components of available reagents have been used for directional induction of neural stem cells into dopaminergic neurons, resulting in a poor ability to repeat experiments. This study sought to investigate whether a homogenate of the substantia nigra of adult rats and/or basic fibroblast growth factor could directionally induce neural stem cells derived from the subventricular zone of embryonic rats to differentiate into dopaminergic neurons. Tyrosine hydroxylase-positive cells were observed exclusively after induction with the homogenate supernatant of the substantia nigra from adult rats and basic fibroblast growth factor for 48 hours in vitro. However, in the groups treated with homogenate supernatant or basic fibroblast growth factor alone, tyrosine hydroxylase expression was not observed. Moreover, the content of dopamine in the culture medium of subventricular zone neurons was significantly increased at 48 hours after induction with the homogenate supernatant of the substantia nigra from adult rats and basic fibroblast growth factor. Experimental findings indicate that the homogenate supernatant of the substantia nigra from adult rats and basic fibroblast growth factor could directionally induce neural stem cells derived from the subventricular zone of embryonic rats to differentiate into dopaminergic neurons in the substantia nigra with the ability to secrete dopamine.
基金supported by the National Natural Science Foundation of China(No.62071240)the Innovation Program for Quantum Science and Technology(No.2021ZD0302901)+1 种基金the Natural Science Foundation of Jiangsu Province(Nos.BK20220804 and BK20231142)the Priority Academic Program Development(PAPD)of Jiangsu Higher Education Institutions.
文摘Event relation extraction plays a crucial role in constructing an event knowledge graph.However,current models only extract trigger words as event ontology representations,and do not consider node type during information aggregation,resulting in low accuracy in event relation extraction.To address these challenges,we propose an event relation extraction model based on heterogeneous graph attention networks and event ontology direction induction.To enhance the completeness of event information,we incorporate argument role information,in addition to trigger words,into the input text.A novel heterogeneous graph attention framework is proposed to reasonably allocate weights to trigger words,argument roles,and text information,and then perform two levels of aggregation,node-level and semantic-level,in sequence.To improve the accuracy of event direction discrimination,we construct an event ontology subgraph that includes trigger words and arguments to aggregate complete event structure information during direction induction.Finally,we evaluate our model on three datasets,TimeBank-Dense,MATRES,and HiEve,and demonstrate that our model outperforms state-of-the-art models by 1.2%,0.5%,and 0.8%,respectively,in terms of the Micro-F1 score.Our proposed model provides a promising solution for event relation extraction and can be applied in various natural language processing applications.
