Labroides dimidiatus has been proven to remove ectoparasites and monogeneans from client fishes and studies showed that they were not infected with the parasite. Due to this, there is a possibility that a defense mech...Labroides dimidiatus has been proven to remove ectoparasites and monogeneans from client fishes and studies showed that they were not infected with the parasite. Due to this, there is a possibility that a defense mechanism against pathogen and parasitic invasion exist in the epidermal mucus which serves as a mechanical as well as biochemical barrier. The study was performed to identify the amino acid and fatty acid components using GC （gas chromatography） and HPLC （high performance liquid chromatography） in epidermal mucus ofL. dimidiatus. The present study revealed 16 components of amino acid and 22 types of fatty acid in epidermal mucus ofL. dimidiatzts. Linoleic acid （C18：2n6c） was the most prominent PUFA （polyunsaturated fatty acid） which contributed approximately 11.69% of total fatty acids. The other major fatty acids are palmitic acid （C 16：0）, oleic acid （C 18： l n9c）, linoledaidic acid （C18：2n6t）, arachidic acid （C20：0）, Gamma-Linoleic acid （C18：3n6） and gadoleic acid （C20：1） which contained reasonable amounts of 9.52%, 8.06%, 6.26%, 8.33%, 6.21% and 9.05% of total fatty acids, respectively. This present study also demonstrated the presence of various amino acids in skin extract. Glycine, glutamine, arginine, asparagin and alanine were found at high concentration of 8.09%, 6.95%, 5.73%, 4.74%, 4.58% respectively. The most abundance percentage of linoleic acid （C18：2n6c） was found to be the metabolic precursor of arachidonic acid （AA） which inducing platelet aggregation, facilitate the blood clotting process and adhesion in endothelial cells during wound healing and might be responsible for rapid tissue growth in L. dimidiatus. It can be concluded that the amino acid and fatty acid profile from the epidermal mucus ofL. dimidiatus contains most of the essential components required to play a possible role in its defense mechanism. Understanding the biochemical properties of L. dimidiatus epidermal mucus in defense mechanism would enable to determine how this fish protect itself from parasitic infection.展开更多
Dear Editor,BCUDCCo African spiny mouse,Acomys dimidiatus,is a mammalian model for regeneration studies because of its ability to functionally regenerate several tissues.As limited regenerative abilities in mammals ar...Dear Editor,BCUDCCo African spiny mouse,Acomys dimidiatus,is a mammalian model for regeneration studies because of its ability to functionally regenerate several tissues.As limited regenerative abilities in mammals are viewed as an antitumor strategy,it is intriguing how Acomys balances regeneration and tumor suppression.In this study,we investigated if Acomys is susceptible to carcinogenesis.We report that,like in mice,two oncogenic hits-activation of oncogenic RasG12v and inactivation of p53 or pRb-are sufficient to malignantly transform skin fibroblasts from Acomys.However,in viuo,Acomys showed resistance to DMBA/TPA-induced skin carcinogenesis.展开更多
文摘Labroides dimidiatus has been proven to remove ectoparasites and monogeneans from client fishes and studies showed that they were not infected with the parasite. Due to this, there is a possibility that a defense mechanism against pathogen and parasitic invasion exist in the epidermal mucus which serves as a mechanical as well as biochemical barrier. The study was performed to identify the amino acid and fatty acid components using GC （gas chromatography） and HPLC （high performance liquid chromatography） in epidermal mucus ofL. dimidiatus. The present study revealed 16 components of amino acid and 22 types of fatty acid in epidermal mucus ofL. dimidiatzts. Linoleic acid （C18：2n6c） was the most prominent PUFA （polyunsaturated fatty acid） which contributed approximately 11.69% of total fatty acids. The other major fatty acids are palmitic acid （C 16：0）, oleic acid （C 18： l n9c）, linoledaidic acid （C18：2n6t）, arachidic acid （C20：0）, Gamma-Linoleic acid （C18：3n6） and gadoleic acid （C20：1） which contained reasonable amounts of 9.52%, 8.06%, 6.26%, 8.33%, 6.21% and 9.05% of total fatty acids, respectively. This present study also demonstrated the presence of various amino acids in skin extract. Glycine, glutamine, arginine, asparagin and alanine were found at high concentration of 8.09%, 6.95%, 5.73%, 4.74%, 4.58% respectively. The most abundance percentage of linoleic acid （C18：2n6c） was found to be the metabolic precursor of arachidonic acid （AA） which inducing platelet aggregation, facilitate the blood clotting process and adhesion in endothelial cells during wound healing and might be responsible for rapid tissue growth in L. dimidiatus. It can be concluded that the amino acid and fatty acid profile from the epidermal mucus ofL. dimidiatus contains most of the essential components required to play a possible role in its defense mechanism. Understanding the biochemical properties of L. dimidiatus epidermal mucus in defense mechanism would enable to determine how this fish protect itself from parasitic infection.
基金supported by grants from the US National Institute on Aging to V.G.and A.S.All institutional guidelines for the care and use of laboratory animals were followedAll animal experiments were approved and performed in accordance with guidelines set forth by the University of Rochester Committee on Animal Resources with protocol number 2017-033(mouse)and 2017-027(Acomys dimidiatus,African spiny mice)All authors have read and approved the manuscript for publication,The data,analytic methods,and study materials will be made available to other researchers for purposes of reproducing the results or replicating the procedure.The RNA-seq raw data and count tables have been deposited in GEO(GSE287010).
文摘Dear Editor,BCUDCCo African spiny mouse,Acomys dimidiatus,is a mammalian model for regeneration studies because of its ability to functionally regenerate several tissues.As limited regenerative abilities in mammals are viewed as an antitumor strategy,it is intriguing how Acomys balances regeneration and tumor suppression.In this study,we investigated if Acomys is susceptible to carcinogenesis.We report that,like in mice,two oncogenic hits-activation of oncogenic RasG12v and inactivation of p53 or pRb-are sufficient to malignantly transform skin fibroblasts from Acomys.However,in viuo,Acomys showed resistance to DMBA/TPA-induced skin carcinogenesis.
