The novel 19 nor l α ,25 dihydroxy vitamin D 3 analogues possessing an ethyl at the 2 position(4 and 5), were synthesized by coupling 25 hydroxy Windaus Grundmann ketone derivative 20 with A ring syntho...The novel 19 nor l α ,25 dihydroxy vitamin D 3 analogues possessing an ethyl at the 2 position(4 and 5), were synthesized by coupling 25 hydroxy Windaus Grundmann ketone derivative 20 with A ring synthons(15 and 19) respectively. The enantioselective synthesis of substituted bicyclic hexanes structure A ring synthons, started from all cis 3,5 dihydroxy 4 ethyl 1 (methoxycarbonyl)cyclohexane via lipase catalyzd asymmetrization, was demonstrated.展开更多
The first stereoselective total synthesis of (-)-3β, 4α-dihydrocy-β-dihydroagarofuran (1) and 3α, 4α-oxidoagarofuran (2) has been described. The key step is the expoxidation of α-agarofuran (6)with dimethyldioxi...The first stereoselective total synthesis of (-)-3β, 4α-dihydrocy-β-dihydroagarofuran (1) and 3α, 4α-oxidoagarofuran (2) has been described. The key step is the expoxidation of α-agarofuran (6)with dimethyldioxirane.展开更多
The conditions of color reaction of Aluminium(Ⅲ) with a new organic reagent 2,3,7 trihydroxy 9 [2,4 dihydroxy)phenylazo]phenylfluorone (ARPF) in the presence of cetyltrimethyammonium bromide(CTMAB) were studied....The conditions of color reaction of Aluminium(Ⅲ) with a new organic reagent 2,3,7 trihydroxy 9 [2,4 dihydroxy)phenylazo]phenylfluorone (ARPF) in the presence of cetyltrimethyammonium bromide(CTMAB) were studied. It was found that Aluminium(Ⅲ) reacts with ARPF and CTMAB in pH 6.5 HAc NaAc buffer solution to form a 1∶2 red complex with maximum absorption at 562 nm .The molar absorptivity is 1.10×10 5 L·mol -1 ·cm -1 . Beer's law is obeyed in the range of 0 5.5 μg Al(Ⅲ) per 25 mL. The method has been applied to determination of Aluminium in the limestone with satisfactory results.展开更多
Orychophragmus violaceus is a Brassicaceae species widely cultivated in China,particularly as a winter cover crop in northern China because of its low-temperature tolerance and low water demand.Recently,O.violaceus ha...Orychophragmus violaceus is a Brassicaceae species widely cultivated in China,particularly as a winter cover crop in northern China because of its low-temperature tolerance and low water demand.Recently,O.violaceus has also been cultivated as a potential industrial oilseed crop because of its abundant 24-carbon dihydroxy fatty acids(diOH-FAs),which contribute to superior high-temperature lubricant properties.In this study,we performed de novo assembly of the O.violaceus genome.Whole-genome synteny analysis of the genomes of its relatives demonstrated that O.violaceus is a diploid that has undergone an extrawhole-genome duplication(WGD)after the Brassicaceae-specific a-WGD event,with a basic chromosome number of x=12.Formation of diOH-FAs is hypothesized to have occurred after the WGD event.Based on the genome and the transcriptome data from multiple stages of seed development,we predicted that OvDGAT1-1 and OvDGAT1-2 are candidate genes for the regulation of diOH-FA storage in O.violaceus seeds.These results may greatly facilitate the development of heat-tolerant and eco-friendly plant-based lubricants using O.violaceus seed oil and improve our understanding of the genomic evolution of Brassicaceae.展开更多
Objecfive To investigate the effect of long-term low-dose1α,25-dihydroxy vitamin D3[1,25(OH)2D3] on rat kidney aquaporin(AQP) 2 expression in 5/6 nephrectomized rats.Methods Twelve
The acid dissociation constants of N-carboxymethyl-N-(p-hydroxy phenyl carbamoyl- methyl)-2,3-dihydroxy-5-carbomethoxy benzylamine(CHDCB) and the stability constants of its 1:1 complexes with alkaline earth, Cd(Ⅱ), ...The acid dissociation constants of N-carboxymethyl-N-(p-hydroxy phenyl carbamoyl- methyl)-2,3-dihydroxy-5-carbomethoxy benzylamine(CHDCB) and the stability constants of its 1:1 complexes with alkaline earth, Cd(Ⅱ), Co(Ⅱ), Ni(Ⅱ), Cu(Ⅱ), Zn(Ⅱ), Fe(Ⅲ),Th(Ⅳ)and U(Ⅵ)ions have been determined at 25.0±0.1℃ and at an ionic strength of 0.1(KNO_3)by pH titration method. The probable coordination sites have also been discussed.展开更多
AIM: To study the chemical constituents of Lomatogonium carinthiacum(Wulfen) Rchb. METHOD: The CHCl3-soluble fraction was separated by chromatography and the structures of the new compounds were elucidated by spectral...AIM: To study the chemical constituents of Lomatogonium carinthiacum(Wulfen) Rchb. METHOD: The CHCl3-soluble fraction was separated by chromatography and the structures of the new compounds were elucidated by spectral experiments. RESULTS: Two new xanthones, 1, 8-dihydroxy-4, 5-dimethoxy-6, 7-methylenedioxyxanthone(1), 1, 4, 8-trimethoxyxanthone-6-O-β-D-glucoronyl-(1→6)O-β-D-glucoside(2) were isolated from the whole plant of Lomatogonium carinthiacum. CONCLUSION: Compounds 1 and 2 are new natural products.展开更多
Sixty-six children with hyperkinesia were treated with the Yizhi (wit-increasing) syrup, af-ter which their scores on behavior dropped, their school records improved, and the rate of appearance of soft neurotic signs ...Sixty-six children with hyperkinesia were treated with the Yizhi (wit-increasing) syrup, af-ter which their scores on behavior dropped, their school records improved, and the rate of appearance of soft neurotic signs lowered, all three changes being significant, giving a total effectiveness rate of 84.8%. After the treatment, examination of the 24-hour urine showed significant increases in its content of norepinephrine (NE), dopamine (DA), 3-4 dihydroxy phenylacetic acid (DOPAC), cyclic adenosine monophosphate (cAMP), and creatinine (Cr).展开更多
Objective:To investigate the essential biochemical indices like 1-hydroxylase and hypocalcaemia in the rats with severe acute pancreatitis and explore the correlation between them.Methods:A total of 120 SPF grade Wist...Objective:To investigate the essential biochemical indices like 1-hydroxylase and hypocalcaemia in the rats with severe acute pancreatitis and explore the correlation between them.Methods:A total of 120 SPF grade Wistar male rats which were in similar physiological status were selected and randomly divided into two groups:sham group(SO group) and severe acute pancreatitis group(SAP group).Then they were divided into 1 h,3 h,6 h,and 12 h subgroups according to the killing lime.The severe acute pancreatitis model was established by retrograde injection of 5%sodium taurocholate.Serum calcium,serum creatinine,serum urea nitrogen and serum amylase were measured at different time.Serum 1,25 dihydroxy vitamin D3 level was determined by enzyme linked immunosorbentassay.The expression of 1-hydroxylase protein in the kidney tissue was determined with Western blotting and immunohistochemistry to observe its location.The pathologic features of the kidney tissue section was observed under light microscope and submicroscopic structure of the proximal convoluted tubule epithelial cell was observed under transmission electron microscope.Results:Compared with the SO group,rats in the SAP group showed continuous pathological injury as time went by.There was significant increase in serum creatinine,serum urea nitrogen and serum amylase in SAP group compared with the SO group 1,3,6,12 hours after the operation(P<0.05).There was significant decrease in serum calcium and 1,25 dihydroxy vitamin D3 3.6,12 hours after the operation(P<0.05).It also showed that the expression of the 1-hydroxylase protein in kidney tissues was upregulated at 1 h.3 h and decreased at 6h,12 h compared with the SO group.The serum calcium,1,25 dihydroxy vitamin D3 and the expression of the 1-hydroxylase protein in kidney tissues of the SAP group showed sustaining decrease.Western blotting showed positive correlation between the 1-hydroxylase expression and serum calcium at 3 h.6 h and 12 h(r=0.976,P<0.001;r=0.948.P<0.001;r=0.742,P=0.001) and also positive correlation between the 1-hydroxylase expression and serum1,25 dihydroxy vitamin D3 at 1 h,3 h,6 h and 12 h(r=0.935,P<0.001;r=0.952,P<0.001;r=0.917.P<0.001:r=0.874,P<0.001).Conclusions:At the early stage of the kidney injury,the expression of 1-hydroxylase in the kidney tissue is reduced with the progress of the disease and the decrease in its activity has a correlation with the hypocalcaemia.展开更多
Benzo(a)pyrene metabolism in human placental microsomes fromsmokers was studied.Benzo(a)pyrene metabolites were separatedusing high pressure liquid chromatographic technique. Reactionof benzo(a)pyrene with microsomal ...Benzo(a)pyrene metabolism in human placental microsomes fromsmokers was studied.Benzo(a)pyrene metabolites were separatedusing high pressure liquid chromatographic technique. Reactionof benzo(a)pyrene with microsomal fraction of placenta fromindividuals who smoke cigarette during pregnency yields 7。展开更多
[Objective] This study aimed to investigate the chemical constituents of Dendrobium thyrsiflorum and determine its bioactive compounds. [Method] The compounds were extracted by 95% alcohol and isolated by column chrom...[Objective] This study aimed to investigate the chemical constituents of Dendrobium thyrsiflorum and determine its bioactive compounds. [Method] The compounds were extracted by 95% alcohol and isolated by column chromatography on silica gel and Sephadex LH-20. Their structures were identified by spectroscopic analysis(1H NMR and13 C NMR). [Result] Seven compounds were obtained and identified as 4, 7-dihydroxy-2-methoxy-9, 10-dihydrophenan-threne(1), syringaldehyde(2),moscatin(3), gigantol(4), batatasin III(5), tristin(6) and stigmasterol(7). [Conclusion] Compounds 1 and 2 were isolated from this medicinal plant for the first time.展开更多
Squaric acid (3,4-dihydroxy-3-cyclobutene-1,2-dione) is a four-membered ring oxocarbon compound. The complexes of squaric acid with some divalent and trivalent metals have been investigated. No molybdenum and tungsten...Squaric acid (3,4-dihydroxy-3-cyclobutene-1,2-dione) is a four-membered ring oxocarbon compound. The complexes of squaric acid with some divalent and trivalent metals have been investigated. No molybdenum and tungsten squarate complexes, however, have been reported. We have found that molybdenum and tungsten squarate complexes can be prepared under selected conditions. Three mmol of sodium tungstate dihydrate in 5 ml of water was mixed with展开更多
The polarographie adsorptive complex waves of rare earth-ALG, indium-ALC and gallium-ALG have been reported. The reaction between germanium and ALC has been studied by Nazarenko et al. using spectrophotometric method....The polarographie adsorptive complex waves of rare earth-ALG, indium-ALC and gallium-ALG have been reported. The reaction between germanium and ALC has been studied by Nazarenko et al. using spectrophotometric method. In the present paper, the polarographic behaviors of germanium-ALC are studied. Three adsorptive complex waves of germanium-ALG have been obtained in NH3-NH4Cl buffer solution. The P4（Ep= -1.06V） is the most sensitive one among them. The limit of detection is 1 × 10-7M for germanium and the sensitivity is higher than photometry by one order.展开更多
The synthesis and characterization of polysulfone (PSF)-polyester (PE) block copolymers were studied. Polysulfone-polyester block copolymers containing segments of PSF and PE (i.e. PPT, PBT, PHT, PDET, PTET) were synt...The synthesis and characterization of polysulfone (PSF)-polyester (PE) block copolymers were studied. Polysulfone-polyester block copolymers containing segments of PSF and PE (i.e. PPT, PBT, PHT, PDET, PTET) were synthesized by interaction of dihydroxy-alcohol (propanediol, butanediol, hexanediol, diethylene-glycol, triethylene-glycol)展开更多
Recently, the research of the antitumor effect of platinum compounds has aroused wide attention. Cis-dichloro-diamine-platinum (DDP) is now used in clinical practice with good results. The antitumor and pharmacologica...Recently, the research of the antitumor effect of platinum compounds has aroused wide attention. Cis-dichloro-diamine-platinum (DDP) is now used in clinical practice with good results. The antitumor and pharmacological studies of DDP produced in China were carried out by us in 1975, and clinical trials were achieved in some other cities of China. After its identification and qualification, the drug was put in produc-展开更多
I. INTRODUCTION In our previous articles, we reported the synthesis of diol monomers such as 1,3-dihydroxy-2-methyl-2-propyl and β, β’-dihydroxyethyl derivatives of thymine and uracil and their polycondensation wit...I. INTRODUCTION In our previous articles, we reported the synthesis of diol monomers such as 1,3-dihydroxy-2-methyl-2-propyl and β, β’-dihydroxyethyl derivatives of thymine and uracil and their polycondensation with the active diamides of benzotriazole such as 1, 1’-(isoph-thaloyl)bisbenzotriazole (IPBBT) and 1, 1’-(sebacoyl)bisbenzotriazole (SeBBT),展开更多
文摘The novel 19 nor l α ,25 dihydroxy vitamin D 3 analogues possessing an ethyl at the 2 position(4 and 5), were synthesized by coupling 25 hydroxy Windaus Grundmann ketone derivative 20 with A ring synthons(15 and 19) respectively. The enantioselective synthesis of substituted bicyclic hexanes structure A ring synthons, started from all cis 3,5 dihydroxy 4 ethyl 1 (methoxycarbonyl)cyclohexane via lipase catalyzd asymmetrization, was demonstrated.
文摘The first stereoselective total synthesis of (-)-3β, 4α-dihydrocy-β-dihydroagarofuran (1) and 3α, 4α-oxidoagarofuran (2) has been described. The key step is the expoxidation of α-agarofuran (6)with dimethyldioxirane.
文摘The conditions of color reaction of Aluminium(Ⅲ) with a new organic reagent 2,3,7 trihydroxy 9 [2,4 dihydroxy)phenylazo]phenylfluorone (ARPF) in the presence of cetyltrimethyammonium bromide(CTMAB) were studied. It was found that Aluminium(Ⅲ) reacts with ARPF and CTMAB in pH 6.5 HAc NaAc buffer solution to form a 1∶2 red complex with maximum absorption at 562 nm .The molar absorptivity is 1.10×10 5 L·mol -1 ·cm -1 . Beer's law is obeyed in the range of 0 5.5 μg Al(Ⅲ) per 25 mL. The method has been applied to determination of Aluminium in the limestone with satisfactory results.
基金supported by the National Natural Science Foundation of China(U20A2034 and 31871659)the China Agriculture Research System(CARS-12)(to C.Z.)E.B.C.was supported by funding from the National Science Foundation(Plant Genome IOS-13-39385).
文摘Orychophragmus violaceus is a Brassicaceae species widely cultivated in China,particularly as a winter cover crop in northern China because of its low-temperature tolerance and low water demand.Recently,O.violaceus has also been cultivated as a potential industrial oilseed crop because of its abundant 24-carbon dihydroxy fatty acids(diOH-FAs),which contribute to superior high-temperature lubricant properties.In this study,we performed de novo assembly of the O.violaceus genome.Whole-genome synteny analysis of the genomes of its relatives demonstrated that O.violaceus is a diploid that has undergone an extrawhole-genome duplication(WGD)after the Brassicaceae-specific a-WGD event,with a basic chromosome number of x=12.Formation of diOH-FAs is hypothesized to have occurred after the WGD event.Based on the genome and the transcriptome data from multiple stages of seed development,we predicted that OvDGAT1-1 and OvDGAT1-2 are candidate genes for the regulation of diOH-FA storage in O.violaceus seeds.These results may greatly facilitate the development of heat-tolerant and eco-friendly plant-based lubricants using O.violaceus seed oil and improve our understanding of the genomic evolution of Brassicaceae.
文摘Objecfive To investigate the effect of long-term low-dose1α,25-dihydroxy vitamin D3[1,25(OH)2D3] on rat kidney aquaporin(AQP) 2 expression in 5/6 nephrectomized rats.Methods Twelve
文摘The acid dissociation constants of N-carboxymethyl-N-(p-hydroxy phenyl carbamoyl- methyl)-2,3-dihydroxy-5-carbomethoxy benzylamine(CHDCB) and the stability constants of its 1:1 complexes with alkaline earth, Cd(Ⅱ), Co(Ⅱ), Ni(Ⅱ), Cu(Ⅱ), Zn(Ⅱ), Fe(Ⅲ),Th(Ⅳ)and U(Ⅵ)ions have been determined at 25.0±0.1℃ and at an ionic strength of 0.1(KNO_3)by pH titration method. The probable coordination sites have also been discussed.
基金supported by the National Natural Science Foundation of China(No.81360673)
文摘AIM: To study the chemical constituents of Lomatogonium carinthiacum(Wulfen) Rchb. METHOD: The CHCl3-soluble fraction was separated by chromatography and the structures of the new compounds were elucidated by spectral experiments. RESULTS: Two new xanthones, 1, 8-dihydroxy-4, 5-dimethoxy-6, 7-methylenedioxyxanthone(1), 1, 4, 8-trimethoxyxanthone-6-O-β-D-glucoronyl-(1→6)O-β-D-glucoside(2) were isolated from the whole plant of Lomatogonium carinthiacum. CONCLUSION: Compounds 1 and 2 are new natural products.
文摘Sixty-six children with hyperkinesia were treated with the Yizhi (wit-increasing) syrup, af-ter which their scores on behavior dropped, their school records improved, and the rate of appearance of soft neurotic signs lowered, all three changes being significant, giving a total effectiveness rate of 84.8%. After the treatment, examination of the 24-hour urine showed significant increases in its content of norepinephrine (NE), dopamine (DA), 3-4 dihydroxy phenylacetic acid (DOPAC), cyclic adenosine monophosphate (cAMP), and creatinine (Cr).
基金supported by Natural Science And Technology Fund of Sichuan Province(sc2812345)
文摘Objective:To investigate the essential biochemical indices like 1-hydroxylase and hypocalcaemia in the rats with severe acute pancreatitis and explore the correlation between them.Methods:A total of 120 SPF grade Wistar male rats which were in similar physiological status were selected and randomly divided into two groups:sham group(SO group) and severe acute pancreatitis group(SAP group).Then they were divided into 1 h,3 h,6 h,and 12 h subgroups according to the killing lime.The severe acute pancreatitis model was established by retrograde injection of 5%sodium taurocholate.Serum calcium,serum creatinine,serum urea nitrogen and serum amylase were measured at different time.Serum 1,25 dihydroxy vitamin D3 level was determined by enzyme linked immunosorbentassay.The expression of 1-hydroxylase protein in the kidney tissue was determined with Western blotting and immunohistochemistry to observe its location.The pathologic features of the kidney tissue section was observed under light microscope and submicroscopic structure of the proximal convoluted tubule epithelial cell was observed under transmission electron microscope.Results:Compared with the SO group,rats in the SAP group showed continuous pathological injury as time went by.There was significant increase in serum creatinine,serum urea nitrogen and serum amylase in SAP group compared with the SO group 1,3,6,12 hours after the operation(P<0.05).There was significant decrease in serum calcium and 1,25 dihydroxy vitamin D3 3.6,12 hours after the operation(P<0.05).It also showed that the expression of the 1-hydroxylase protein in kidney tissues was upregulated at 1 h.3 h and decreased at 6h,12 h compared with the SO group.The serum calcium,1,25 dihydroxy vitamin D3 and the expression of the 1-hydroxylase protein in kidney tissues of the SAP group showed sustaining decrease.Western blotting showed positive correlation between the 1-hydroxylase expression and serum calcium at 3 h.6 h and 12 h(r=0.976,P<0.001;r=0.948.P<0.001;r=0.742,P=0.001) and also positive correlation between the 1-hydroxylase expression and serum1,25 dihydroxy vitamin D3 at 1 h,3 h,6 h and 12 h(r=0.935,P<0.001;r=0.952,P<0.001;r=0.917.P<0.001:r=0.874,P<0.001).Conclusions:At the early stage of the kidney injury,the expression of 1-hydroxylase in the kidney tissue is reduced with the progress of the disease and the decrease in its activity has a correlation with the hypocalcaemia.
文摘Benzo(a)pyrene metabolism in human placental microsomes fromsmokers was studied.Benzo(a)pyrene metabolites were separatedusing high pressure liquid chromatographic technique. Reactionof benzo(a)pyrene with microsomal fraction of placenta fromindividuals who smoke cigarette during pregnency yields 7。
基金Supported by Grants from the National Natural Science Foundation of China(No.21202066)the Natural Science Foundation of Yunnan Province,China(No.2012FB156)the Open Research Foundation of Yunnan Key Laboratory of Pharmacology for Natural Products(No.2014G011)
文摘[Objective] This study aimed to investigate the chemical constituents of Dendrobium thyrsiflorum and determine its bioactive compounds. [Method] The compounds were extracted by 95% alcohol and isolated by column chromatography on silica gel and Sephadex LH-20. Their structures were identified by spectroscopic analysis(1H NMR and13 C NMR). [Result] Seven compounds were obtained and identified as 4, 7-dihydroxy-2-methoxy-9, 10-dihydrophenan-threne(1), syringaldehyde(2),moscatin(3), gigantol(4), batatasin III(5), tristin(6) and stigmasterol(7). [Conclusion] Compounds 1 and 2 were isolated from this medicinal plant for the first time.
文摘Squaric acid (3,4-dihydroxy-3-cyclobutene-1,2-dione) is a four-membered ring oxocarbon compound. The complexes of squaric acid with some divalent and trivalent metals have been investigated. No molybdenum and tungsten squarate complexes, however, have been reported. We have found that molybdenum and tungsten squarate complexes can be prepared under selected conditions. Three mmol of sodium tungstate dihydrate in 5 ml of water was mixed with
文摘The polarographie adsorptive complex waves of rare earth-ALG, indium-ALC and gallium-ALG have been reported. The reaction between germanium and ALC has been studied by Nazarenko et al. using spectrophotometric method. In the present paper, the polarographic behaviors of germanium-ALC are studied. Three adsorptive complex waves of germanium-ALG have been obtained in NH3-NH4Cl buffer solution. The P4（Ep= -1.06V） is the most sensitive one among them. The limit of detection is 1 × 10-7M for germanium and the sensitivity is higher than photometry by one order.
文摘The synthesis and characterization of polysulfone (PSF)-polyester (PE) block copolymers were studied. Polysulfone-polyester block copolymers containing segments of PSF and PE (i.e. PPT, PBT, PHT, PDET, PTET) were synthesized by interaction of dihydroxy-alcohol (propanediol, butanediol, hexanediol, diethylene-glycol, triethylene-glycol)
文摘Recently, the research of the antitumor effect of platinum compounds has aroused wide attention. Cis-dichloro-diamine-platinum (DDP) is now used in clinical practice with good results. The antitumor and pharmacological studies of DDP produced in China were carried out by us in 1975, and clinical trials were achieved in some other cities of China. After its identification and qualification, the drug was put in produc-
文摘I. INTRODUCTION In our previous articles, we reported the synthesis of diol monomers such as 1,3-dihydroxy-2-methyl-2-propyl and β, β’-dihydroxyethyl derivatives of thymine and uracil and their polycondensation with the active diamides of benzotriazole such as 1, 1’-(isoph-thaloyl)bisbenzotriazole (IPBBT) and 1, 1’-(sebacoyl)bisbenzotriazole (SeBBT),
