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探讨Array 2905 Digitizer在数字化教学中的作用 被引量:1
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作者 蒋翔 钟国康 冯晓源 《上海生物医学工程》 2005年第2期103-106,共4页
关键词 ARRAY 2905 digitizeR 数字化教学 医学影像胶片 医学教育 数据库 医疗教学科研
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Digitized Outcrop Geomodeling of Ramp Shoals and its Reservoirs: as an Example of Lower Triassic Feixianguan Formation of Eastern Sichuan Basin 被引量:10
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作者 QIAO Zhanfeng SHEN Anjiang +5 位作者 ZHENG Jianfeng Xavier JANSON CHANG Shaoying WANG Xiaofang CHEN Yana LIAO Yisha 《Acta Geologica Sinica(English Edition)》 SCIE CAS CSCD 2017年第4期1395-1412,共18页
Numerous hydrocarbon accumulations are found in ramp crest shoals worldwide and therefore this depositional setting has a high potential of being the hydrocarbon reservoir. In this paper, we combined digital outcrop g... Numerous hydrocarbon accumulations are found in ramp crest shoals worldwide and therefore this depositional setting has a high potential of being the hydrocarbon reservoir. In this paper, we combined digital outcrop geology and traditional geological mapping to build an outcrop-based geocellular model of the ramp-crest shoal complex of the Lower Triassic Feixianguan Formation in the Eastern Sichuan Basin. The outcrop model serves as an analogue for the subsurface reservoir of the Feixianguan Formation and illustrates the complexity of the lithofacies types, stratigraphic architecture, and reservoir heterogeneities at a scale below conventional subsurface data resolution. The studied ramp -crest shoal complex consists of thirteen types of lithofacies that can be grouped into three facies-groups corresponding to subtidal intraclastic shoal, sub- to inter-tidal oolitic shoal, and tidal flat depositional environments respectively. The stratigraphic architecture of the shoal complex shows mostly a strong progradation of the high energy facies associated with an overall decrease of accommodation space associated with relative sea level still stand. Two reservoir facies associations have been recognized. The first one consists of supratidai dolomudstone and upper intertidal partially dolomitized oolitic packstone with anhydrite or nodules. These facies were deposited above the high energy oolitic grainstones and occurs as thin-bedded and laterally continuous layers, characterized by high porosity and low permeability. The second reservoir facies association is composed of intertidal crystalline dolomite and subtidal intraclastic bindstone that occurs stratigraphically below the oolitic grainstones. These deposits consist of massive laterally discontinuously beds, and are characterized by high porosity and high permeability. Both types of reservoir facies tend to be stacked vertically and migrated laterally with the progradation of the shoal complex. The construction of the outcrop-based 3D geological model provide a description and quantification of the facies distribution within a robust stratigraphic framework and the style and amount of reservoir heterogeneities associated with a ramp-crest shoal complex reservoir such as the one found in Lower Triassic Feixianguan Formation and Cambrian Longwangmiao Formation in Sichuan Basin or other ramp-crest reservoir worldwide. 展开更多
关键词 digitized outcrop geomodeling ramp shoal RESERVOIR Feixianguan Formation Sichuan Basin
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Design and offline processing of an ultrafast digitizer based on internal cascaded DRS4 被引量:2
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作者 Ya-Fei Du Jun Wu +4 位作者 Chen Yuan Bo Yang Cen-Ming Ye Chuan-Fei Zhang Yi-Nong Liu 《Nuclear Science and Techniques》 SCIE CAS CSCD 2019年第8期35-41,共7页
In this paper, we present an ultrafast digitizer utilizing the DRS4 switched capacitor array applicationspecific integrated circuit to achieve an ultrafast sampling speed of at most 5 GS/s. We cascaded all eight chann... In this paper, we present an ultrafast digitizer utilizing the DRS4 switched capacitor array applicationspecific integrated circuit to achieve an ultrafast sampling speed of at most 5 GS/s. We cascaded all eight channels(sub-channels) of a single DRS4 chip for increased storage depth. The digitizer contains four DRS4 chips, a quadchannel analog-to-digital converter,a controlling fieldprogrammable gate array, a PXI interface, and an SFP+connector. Consequently, each DRS4 channel has a depth of 8192 points and a vertical resolution of 14 bits. The readout sequences should be broken into several segments and then reordered to obtain the correct sequential data sets, and this offline procedure varies in different readout modes. This paper describes the design and implementation of the hardware;in particular, the respective processing procedures are described in detail. Furthermore, the offset error is calibrated and corrected to improve the precision of the captured waveform in both single-channel and highresolution modes. 展开更多
关键词 ULTRAFAST digitizeR DRS4 CASCADE READOUT
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Resolution of Digitized Conjugate Tooth-Face Surface Based on the Theory of Digitized Conjugate Surfaces 被引量:1
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作者 XIAOLai-yuan LIAODao-xun YIChuan-yun 《International Journal of Plant Engineering and Management》 2004年第1期1-7,共7页
According to the principle of meshing engagement and the theory of the digitized conjugate surface, this paper applies the software Conjugater-1.0 that is developed by ourselves to compute, respectively, the digitized... According to the principle of meshing engagement and the theory of the digitized conjugate surface, this paper applies the software Conjugater-1.0 that is developed by ourselves to compute, respectively, the digitized conjugate curved surfaces of the straight-tooth surface and drum-tooth surface, which will establish the theoretical and technical foundation for digitized engaging analysis, simulation, and digitized manufacturing technology of the diversified gears. 展开更多
关键词 digitized conjugate surface generating method SIMULATION tooth-face
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Graph Digitizer在计量检定工作中的应用探讨 被引量:1
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作者 黎晓玲 任昀 陈扬 《计量与测试技术》 2014年第2期1-2,共2页
以"液相色谱仪"梯度误差计量检定数据处理为例,介绍应用GetData Graph Digitizer对目标图解析,获取原始数据,后运用Excel进行数据处理,得到梯度误差及判定结果。这不仅能提高计算结果的正确性,还能广泛运用到其他检定项目以... 以"液相色谱仪"梯度误差计量检定数据处理为例,介绍应用GetData Graph Digitizer对目标图解析,获取原始数据,后运用Excel进行数据处理,得到梯度误差及判定结果。这不仅能提高计算结果的正确性,还能广泛运用到其他检定项目以及数据复核等质量监控工作中去。 展开更多
关键词 GRAPH digitizeR 计量检定 数据处理
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Theory of Digitized Conjugate Surface and Solution to Conjugate Surface
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作者 XiaoLai-yuan LiaoDao-xun YiChuan-yun 《Wuhan University Journal of Natural Sciences》 EI CAS 2004年第2期183-187,共5页
In order to meet the needs of designing and processing digitized surfaces, the method to spreading digitized surface has been proposed. The key technique is to solve the problem of digitized conjugate surface. In the ... In order to meet the needs of designing and processing digitized surfaces, the method to spreading digitized surface has been proposed. The key technique is to solve the problem of digitized conjugate surface. In the paper, the digitized conjugate surface was theoretically investigated, and the solution of conjugate surface based on digitized surface was also studied. The digitized conjugate surface theory was then proposed, and applied to build the model of solving conjugate surface based on digitized surface. A corresponding algorithm was developed. This paper applies the software Conjugater-1.0 that is developed by ourselves to compute the digitized conjugate surfaces of the drum-tooth surface. This study provides theoretical and technical bases for analyzing engagement of digitized surface, simulation and numerical processing technique. Key words digitized conjugate surface - generating method - simulation CLC number O 186.1 - TH 122 Foundation item: Supported by the National Natural Science Foundation of China (50075031)Biography: Xiao Lai-yuan (1957-), male, Professor, Ph. D candidate, research direction: machanical design & theory, digital technology 展开更多
关键词 digitized conjugate surface generating method SIMULATION
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Mobile mini-fluorimeter for antibiotic aptasensing based on surface-plasmonic effect of burlike nanogolds enhanced by digitized imaging diagnosis
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作者 Tiantian Man Fulin Zhu +4 位作者 Yaqi Huang Yuhao Piao Yan Su Shengyuan Deng Ying Wan 《Chinese Chemical Letters》 SCIE CAS CSCD 2024年第5期418-422,共5页
Antibiotic abuse now poses a grave threat to global ecology and bestirs public concerns about the residue issue in daily necessities.The traceability measurements along supply chain or logistic circulation have become... Antibiotic abuse now poses a grave threat to global ecology and bestirs public concerns about the residue issue in daily necessities.The traceability measurements along supply chain or logistic circulation have become increasingly essential given the labile nature of diverse synthetic residuals on site.In an attempt to answer this urgency,here a miniaturized fluorometric aptasensor prototype was contrived that catered to the point-of-care screening norm for two typical additives:chloramphenicol and enrofloxacin.The key target-indicating module worked in vitro based on the competitive binding-induced fluorescence recovery of fluorescein-labeled aptamers,which were photobleached beforehand in the format of double helix on burlike nanogold carriers.The“prickly”geometry of the latter not just enriched the capture probes at preferentially substrate-accessible spires;but also contributed to a tip-enhanced surface plasmon effect,sensitizing the signal-on during the duplex dissociation even at nanomolar threshold of the analytes.On the other hand,to encompass a full portable,a set of optical devices were mounted within a 3D-printed cartridge(adaptor)to converge the light beam and route it towards the detector,for which the smartphone camera came up in handy with a home-developed App for calibrating the emissive brightness.Enlightened by the high-dynamic-range compression,an imaging diagnostic algorithm was built in to grid and digitize each slide in the album for augmented detection performance.Thus,a novel bio-to-silico integration was invented that capable of in situ rapid reporting on the antibiotic presence with high sensitivity and selectivity.Further field practices in spiked milk on sales proved the precision and rudimentary feasibility of the well-assembled model of appliance,thus holding nice prospects in nonexpert(e.g.,family and local community)utilities for foodborne antibiotic identification. 展开更多
关键词 Chloramphenicol and enrofloxacin APTASENSOR Gold nanoburs Smartphone fluorimetric suite digitized image postprocessing
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A New Method to Digitize DNA Sequence
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作者 Xiaofei Xu Feng Zhu 《Journal of Biosciences and Medicines》 2017年第3期7-12,共6页
The global description uses composition, transition and distribution to describe an amino acid sequence and has been widely adopted in various fields. Here we integrate it with properties of nucleic acid and form a ne... The global description uses composition, transition and distribution to describe an amino acid sequence and has been widely adopted in various fields. Here we integrate it with properties of nucleic acid and form a new method to digitize DNA sequence. Through this method we can use a 39-dimession vector to represent a DNA sequence. We use the exon-1 of β-Globin genes of eight species to verify this method and compare with other methods. A similar result with other method proves that this method is persuading. This method provides a new strategy to digitize DNA sequence and generates DNA sequence descriptor vector. It is different from other methods and this method only produces a 39-dimession vector and not depends on the length of DNA sequence. 展开更多
关键词 DNA SEQUENCE CHARACTERISTIC SEQUENCE Global DESCRIPTION digitize DNA
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Neural network approach for modification and fitting of digitized data in reverse engineering~
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作者 鞠华 王文 +1 位作者 谢金 陈子辰 《Journal of Zhejiang University Science》 EI CSCD 2004年第1期75-80,共6页
Reverse engineering in the manufacturing field is a process in which the digitized data are obtained from an existing object model or a part of it, and then the CAD model is reconstructed. This paper presents an RBF n... Reverse engineering in the manufacturing field is a process in which the digitized data are obtained from an existing object model or a part of it, and then the CAD model is reconstructed. This paper presents an RBF neural network approach to modify and fit the digitized data. The centers for the RBF are selected by using the orthogonal least squares learning algorithm. A mathematically known surface is used for generating a number of samples for training the networks. The trained networks then generated a number of new points which were compared with the calculating points from the equations. Moreover, a series of practice digitizing curves are used to test the approach. The results showed that this approach is effective in modifying and fitting digitized data and generating data points to reconstruct the surface model. 展开更多
关键词 Reverse engineering digitized data Neural network modification and fitting
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National Standard Prepared to Be Established for the Field of Digitized Architecture
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《China Standardization》 2004年第3期11-,共1页
关键词 BE National Standard Prepared to Be Established for the Field of digitized Architecture
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Getdata Graph Digitizer在物理教学中的应用——以自由落体运动为例
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作者 高小燕 《物理通报》 2018年第6期55-58,共4页
频闪照相技术能直观便捷地展现物体的运动特征,在物理教学中的使用越来越普遍.以自由落体运动为例,用数码相机高速拍摄运动物体的频闪照片,然后利用Getdata Graph Digitizer等图像分析软件能从这些频闪照片中迅速准确地提取运动过程的... 频闪照相技术能直观便捷地展现物体的运动特征,在物理教学中的使用越来越普遍.以自由落体运动为例,用数码相机高速拍摄运动物体的频闪照片,然后利用Getdata Graph Digitizer等图像分析软件能从这些频闪照片中迅速准确地提取运动过程的详细数据,以便于利用计算机软件对该运动特征进行定量分析,可以显著提高实验教学课堂效率. 展开更多
关键词 频闪照片 Getdata GRAPH digitizer软件 自由落体运动 物理教学
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A LINEAR APPROACH TO METRIC CIRCUMFERENCE COMPUTATIO FOR DIGITIZED CONVEX SHAPES 被引量:3
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作者 Chen Ken Zhao Pan Yang Rener 《Journal of Electronics(China)》 2008年第4期572-575,共4页
Metric measurement of digitized shapes is commonly applied in optical measuring systems.In this letter,three shape-related factors defined by the authors are used in the construction of amultiple linear regression mod... Metric measurement of digitized shapes is commonly applied in optical measuring systems.In this letter,three shape-related factors defined by the authors are used in the construction of amultiple linear regression model which is utilized to compute the circumference of the convex shapes inmillimeter unit.The model is first built upon the relationship hypothesis and then its adequacy ismathematically validated.The results of applying the developed model to the given number of convexshapes in a finite circumferential length range suggest that,in terms of percent error,the model pre-cision is to satisfaction by being within±4%.The test also shows the model’s robustness against theshape’s orientation anisotropy. 展开更多
关键词 Metric measurement Circumference computation Digital image analysis Modeling
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Multimodal Deep Neural Networks for Digitized Document Classification
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作者 Aigerim Baimakhanova Ainur Zhumadillayeva +4 位作者 Bigul Mukhametzhanova Natalya Glazyrina Rozamgul Niyazova Nurseit Zhunissov Aizhan Sambetbayeva 《Computer Systems Science & Engineering》 2024年第3期793-811,共19页
As digital technologies have advanced more rapidly,the number of paper documents recently converted into a digital format has exponentially increased.To respond to the urgent need to categorize the growing number of d... As digital technologies have advanced more rapidly,the number of paper documents recently converted into a digital format has exponentially increased.To respond to the urgent need to categorize the growing number of digitized documents,the classification of digitized documents in real time has been identified as the primary goal of our study.A paper classification is the first stage in automating document control and efficient knowledge discovery with no or little human involvement.Artificial intelligence methods such as Deep Learning are now combined with segmentation to study and interpret those traits,which were not conceivable ten years ago.Deep learning aids in comprehending input patterns so that object classes may be predicted.The segmentation process divides the input image into separate segments for a more thorough image study.This study proposes a deep learning-enabled framework for automated document classification,which can be implemented in higher education.To further this goal,a dataset was developed that includes seven categories:Diplomas,Personal documents,Journal of Accounting of higher education diplomas,Service letters,Orders,Production orders,and Student orders.Subsequently,a deep learning model based on Conv2D layers is proposed for the document classification process.In the final part of this research,the proposed model is evaluated and compared with other machine-learning techniques.The results demonstrate that the proposed deep learning model shows high results in document categorization overtaking the other machine learning models by reaching 94.84%,94.79%,94.62%,94.43%,94.07%in accuracy,precision,recall,F-score,and AUC-ROC,respectively.The achieved results prove that the proposed deep model is acceptable to use in practice as an assistant to an office worker. 展开更多
关键词 Document categorization deep learning machine learning CLASSIFICATION DIGITIZATION
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Digitizing the Undigitized: Converting Traditional Archaeological Records into Computerized, Three-Dimensional Site Reconstruction
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作者 Nira Alperson-Afil 《Journal of Geographic Information System》 2019年第6期747-765,共19页
Archaeological excavation involves disintegration, removal, and reassembly of the archaeological record;as such it is considered by many to be an unrepeatable, destructive activity. This perception has contributed to ... Archaeological excavation involves disintegration, removal, and reassembly of the archaeological record;as such it is considered by many to be an unrepeatable, destructive activity. This perception has contributed to an advancement in archaeological practice, namely, the development of computerized recording systems that digitally record archaeological excavations spatially and volumetrically during fieldwork. This paper is concerned with those archaeological sites where digital field recording has not been done. These sites, recorded by traditional methods, should not be excluded from attempts to restructure the spatial, volumetric, and stratigraphic archaeological data. A thorough methodology for the conversion of traditional records into digitized data is presented, including the detailed procedures required for three-dimensional plotting of recorded data—both the excavated material and the drawn site maps and cross-sections. Finally, the use of these methods is demonstrated on a complex Early to Middle Pleistocene site, illustrating the benefits of digitization and three-dimensional reconstruction in resolving stratigraphic and spatial questions. 展开更多
关键词 Digital ARCHAEOLOGY GEOGRAPHIC Information Systems Archaeological Recording Methods THREE-DIMENSIONAL RECONSTRUCTION Model
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Study of Tea Digitized Chromatographic Fingerprint Spectra Using Micellar Electrokinetic Chromatography 被引量:3
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作者 宋冠群 林金明 +1 位作者 屈锋 董文举 《Chinese Journal of Chemistry》 SCIE CAS CSCD 2003年第10期1325-1329,共5页
This paper described the principle of digitized chromatographic fingerprint spectrum and established digitized chromatographic fingerprint spectra of ten brands of Chinese famous tea by the micellar electrokinetic chr... This paper described the principle of digitized chromatographic fingerprint spectrum and established digitized chromatographic fingerprint spectra of ten brands of Chinese famous tea by the micellar electrokinetic chromatography. This work was done using a 25 mmol·L -1 sodium dodecylsulfate in a 20 mmol·L -1 borate (pH 7 0) solution as running buffer, 20 kV applied potential and detection at 280 nm. The chromatographic fingerprint spectra were digitized by the relative retention value ( α ) and the relative area ( S r), and were analyzed to identify the tea samples. In the absence of the standard samples, the present method was easy setup and inexpensive, and provided the applicable information for the quality assessment of teas. 展开更多
关键词 tealeaves micellar electrokinetic chromatography digitized chromatographic fingerprint spectrum quality assessment
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EU’s DPP countdown begins! South Asian companies in fast lane, Chinese fifirms gear up
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作者 Zhao Xinhua 《China Textile》 2026年第1期22-25,共4页
Background In July 2024,the European Union's Ecodesign for Sustainable Products Regulation,or ESPR,officially came into effect.This regulation mandates that products entering the EU market must use a Digital Produ... Background In July 2024,the European Union's Ecodesign for Sustainable Products Regulation,or ESPR,officially came into effect.This regulation mandates that products entering the EU market must use a Digital Product Passport,known as DPP,to disclose their compliance certifications,environmental impact,and supply chain information.Failure to comply may result in exclusion from the EU market. 展开更多
关键词 eu chinese firms digital product passportknown espr south asian companies DPP sustainable products digital product passport
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Mapping research trends and competency domains in nursing-related digital and artificial intelligence technologies:A bibliometric analysis
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作者 Kanjanee Phanphairoj Wasinee Wisesrith Sutthisan Chumwichan 《International Journal of Nursing Sciences》 2026年第1期36-44,I0003,I0004,共11页
Objectives This study aimed to explore the research trends,thematic structures,and core competency domains in the field of nursing-related digital and artificial intelligence(AI)technologies.Methods A bibliometric ana... Objectives This study aimed to explore the research trends,thematic structures,and core competency domains in the field of nursing-related digital and artificial intelligence(AI)technologies.Methods A bibliometric analysis was conducted in accordance with the PRISMA 2020 statement.Peer-reviewed articles published in English from 2015 to 2025 were retrieved from Scopus,Web of Science,and PubMed.Thematic clustering was conducted using the Louvain algorithm and cosine similarity.A subset of 66 frequently cited articles was then qualitatively synthesized to capture core competencies across clusters.Results A total of 83,807 articles were included for bibliometric analysis.Of these,66 articles were chosen for thematic analysis.Five major thematic clusters were identified:remote care in primary settings,oncology and palliative care,nurse education and training,safety and quality in nursing practice,and geriatric and dementia care.Additionally,four competency domains were identified:telehealth and remote communication,health systems and informatics,digital tools in practice,and AI-powered decision support.A clear shift in research focus was observed,with the emphasis transitioning from foundational digital skills before the COVID-19 pandemic to more advanced competencies during the post-pandemic digital transformation,encompassing ethical reasoning,immersive technology use,and AI integration.Conclusions Integrating digital and AI technologies is reshaping nursing practice across various thematic areas and competency domains,highlighting a transition from foundational digital tasks to AI-supported decision-making and ethically informed technology use.This study provides a structured overview of evolving competencies in digital nursing and synthesizes evidence to support future research,curriculum design,and policy planning. 展开更多
关键词 Artificial intelligence competence Bibliometric analysis Digital competence Nursing competency domains Post-pandemic digital transformation
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Neutron time-of-flight spectroscopy measurement using a waveform digitizer
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作者 刘龙祥 王宏伟 +8 位作者 马余刚 曹喜光 蔡翔舟 陈金根 张桂林 韩建龙 张国强 胡继峰 王小鹤 《Chinese Physics C》 SCIE CAS CSCD 2016年第5期70-74,共5页
The photoneutron source (PNS, phase 1), an electron linear accelerator (linac)-based pulsed neutron facility that uses the time-of-flight (TOF) technique, was constructed for the acquisition of nuclear data from... The photoneutron source (PNS, phase 1), an electron linear accelerator (linac)-based pulsed neutron facility that uses the time-of-flight (TOF) technique, was constructed for the acquisition of nuclear data from the Thorium Molten Salt Reactor (TMSR) at the Shanghai Institute of Applied Physics (SINAP). The neutron detector signal used for TOF calculation, with information on the pulse arrival time, pulse shape, and pulse height, was recorded by using a waveform digitizer (WFD). By using the pulse height and pulse-shape discrimination (PSD) analysis to identify neutrons and "y-rays, the neutron TOF spectrum was obtained by employing a simple electronic design, and a new WFD-based DAQ system was developed and tested in this commissioning experiment. The DAQ system developed is characterized by a very high efficiency with respect to millisecond neutron TOF spectroscopy. 展开更多
关键词 neutron spectroscopy scintillator waveform digitizer pulse-shape discrimination (PSD) time-of-flight (TOF)
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Theoretical Logic and Practical Pathways for Distance Open Education Empowering the Enhancement of Digital Literacy among Rural Cadres
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作者 Ming LU Ji TANG Yuxiao ZHANG 《Asian Agricultural Research》 2026年第2期37-41,共5页
At present, enhancing the digital literacy of village cadres faces practical constraints such as governance capability gaps induced by the second-level digital divide, the absence of a systematic cultivation system, i... At present, enhancing the digital literacy of village cadres faces practical constraints such as governance capability gaps induced by the second-level digital divide, the absence of a systematic cultivation system, incomplete last-kilometer implementation for translating skills into practice, and inadequate incentive mechanisms. As a novel educational form deeply integrating digital information technology with teaching and learning, distance open education provides multi-directional empowerment for enhancing village cadres digital literacy. This is achieved through open learning methods, the application of modern digital technologies, tailored learning programs, and the establishment of "overpass bridges" for translating learning outcomes into practice. Based on this, it is essential to further streamline the pathways for educating and cultivating village cadres, vigorously promote the digitalization of teaching, practice, and support services, develop systematic and localized digital education resources, and actively explore and establish a complementary credit bank system for their digital literacy cultivation. 展开更多
关键词 Distance open education Digital literacy Village cadres Rural digital governance
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Detection Sensitivity of Chip Digital PCR for Staphylococcus aureus
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作者 Yunxia WANG Xiaxia HOU +1 位作者 Xiaoli WU Wang LIU 《Asian Agricultural Research》 2026年第1期46-50,60,共6页
[Objectives]This study aimed to evaluate the detection sensitivity of Staphylococcus aureus in dairy products utilizing the chip digital PCR(cdPCR)technique.[Methods]Specific primers and probes were designed and synth... [Objectives]This study aimed to evaluate the detection sensitivity of Staphylococcus aureus in dairy products utilizing the chip digital PCR(cdPCR)technique.[Methods]Specific primers and probes were designed and synthesized based on the conserved sequence of the heat-resistant nuclease gene nuc of S.aureus.cdPCR was employed to detect S.aureus,and the sensitivity of this technique was systematically assessed in samples exhibiting low levels of contamination.[Results]cdPCR demonstrated precise quantification when the initial concentration of the sample enrichment solution was equal to or greater than 50 CFU/mL.The detection dynamic range extended across at least five orders of magnitude,with a minimum DNA detection limit of 0.2304 pg/μL.In artificially contaminated cheese samples,the method s lower limit of quantification for detecting S.aureus was 8×10^(2) CFU/g.Regression analysis demonstrated that the gene copy number concentration measured by cdPCR exhibited a strong linear correlation with bacterial contamination concentration across a broad range.[Conclusions]The cdPCR method developed in this study demonstrates high sensitivity and robust quantitative capabilities,offering a reliable technical approach for the precise detection of low-level S.aureus contamination in dairy products. 展开更多
关键词 STAPHYLOCOCCUS AUREUS CHIP DIGITAL PCR (cdPCR) Sensitivity
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