The aim of the present study was to evaluate the effects of baobab seed washing and origin on the chemical composition of the oil extracted by pressing. Six (6) oil samples were obtained from seeds of three (3) geogra...The aim of the present study was to evaluate the effects of baobab seed washing and origin on the chemical composition of the oil extracted by pressing. Six (6) oil samples were obtained from seeds of three (3) geographical origins. The identification and quantification of fatty acids and the polyphenolic profile were carried out by GC-MS and HPLC-UV, respectively. Analysis of fatty acid methyl esters allowed the identification and quantification of 18 fatty acids. Oils from unwashed seeds were richer in palmitic (C16:0), stearic (C18:0), oleic (C18:1) and arachidic (C20:0) acids. In addition, HPLC-UV analysis at 279 nm shows that oils from unwashed seeds are richer in tyrosol, hydroxytyrosol and caffeic acid. With regard to the polyphenolic profile, gallic acid and quercetin were not detected in these baobab oils. Principal component analysis of fatty acid and phenolic compound content showed that oils from unwashed seeds would best preserve their chemical and associated potential bioactive characteristics.展开更多
Adansonia digitata L.(Malvaceae) is commonly known as baobab tree native to Africa.Baobab is a multi-purpose tree which offers protection and provides food,clothing and medicine as well as raw material for many useful...Adansonia digitata L.(Malvaceae) is commonly known as baobab tree native to Africa.Baobab is a multi-purpose tree which offers protection and provides food,clothing and medicine as well as raw material for many useful items.The fruit pulp,seeds,leaves,flowers.roots,and bark of baobab are edible and they have been studied by scientists for their useful properties.The fruit pulp have very high vitamin C.calcium,phosphorus,carbohydrates,fibers,potassium,proteins and lipids content,which can be used in seasoning as an appetizer and also make juices.Seeds contain appreciable quantities of phosphorus,magnesium,zinc,sodium,iron,manganese,whereas they have high levels of lysine,thiamine,calcium and iron.Baobab has numerous biological properties including antimicrobial,anti-malarial,diarrhoea,anaemia,asthma,antiviral,anti-oxidant and anti-inflammatory activities amongst others.Phytochemical investigation revealed the presence of flavonoids.phytosterols.amino acids,fatty acids,vitamins and minerals.The review summarizes the information on various aspects of traditional information,taxonomic description,medicinal properties and importantly nutritional value.展开更多
Objective:To clone,express and purify a putative parasitic nematode specific protein of Setaria digitata(S.digitata),filarial nematode that infects livestock and cause significant economic losses in Far East and Asia ...Objective:To clone,express and purify a putative parasitic nematode specific protein of Setaria digitata(S.digitata),filarial nematode that infects livestock and cause significant economic losses in Far East and Asia to he used for structural and functional analyses.Methods:To characterize uneharacterized gene of,S.digitata(SDUG),the herterologous expression of SDUG was carried out in the pET[cloned into pET45b(+)]expression system initially and co-expression of SDUC using chaperoiie plasmids pG-KJE8,pGro 7,pKJE7,pG-Tf2 and pTf16 containing chapcrone proteins of dnaK-dnaJ-grpE-groES-gro-E,groES-groEL,dnaK-dnaJ-grpE,groES-groEL-tig,and tig respectively,was carried out subsequently.Results:Expression of SDUG was seen when Escherichia coli strain BI.21(DE3)is used,while concentrating protein largely into the insoluble fraction.The co-expression of SDUG using chaperoiie plasmid mediated system indicated a significant increase of the protein in the soluble fraction.Of the chaperon plasniid sets,the highest amount of recombinant SDUP in the soluble fraction was seen when pGro7 was used in the presence of2 mg/mL L-arabinosc and 0.6M IPTG concentration in the culture medium and for 3 h of incubation at the temperature of 28℃.Recombinant SDUG was purified both from soluble and insoluble fractions using Ni affinity chromatography.SDS-PAGE and western blot analyses of these proteins revealed a single band having expected size of^24 kDa.Conclusions:SDUG seems to be more aggregate-prone and hydrophobic in nature and such protein can make soluble by correct selecting the inducer concentrations and induction temperature and its duration.展开更多
In this study, the effect of extraction processes on the physicochemical characteristics and antioxidant potential of baobab (Adansonia digitata L.) seed oil was evaluated. The oils were extracted, on the one hand, by...In this study, the effect of extraction processes on the physicochemical characteristics and antioxidant potential of baobab (Adansonia digitata L.) seed oil was evaluated. The oils were extracted, on the one hand, by cold pressing, and on the other hand, with three types of organic solvents (acetone, chloroform, n-hexane). The recorded results indicated that the extraction yield of baobab oil was significantly impacted by both the extraction method and the polarity of the solvent used. In addition, chloroform provides the best extraction yield (40.12 ± 0.607). However, extraction by cold pressure preserves at best the physicochemical and bioactive properties of the extracted oils. Indeed, the pressing oil contains a content of phenolic compounds (0.047 ± 0.0024 mgEAG/g of oil) and a very high radical scavenging activity (DPPH) (31.71% ± 0.61%). For the various oils extracted, the minimum and maximum values were 0.50 and 3.17 mEq?kg-1;56.26 and 99.113 mgI2?100 g-1;1.457 and 1.465;205.37 and 233.587 mgKOH/g respectively for the peroxide, iodine, refractive and saponification values. The color parameters (L*, a* and b*) of the oils also differ depending on the nature of the organic solvent used. Principal component analysis (PCA) and correlation analysis were performed on the physicochemical properties and the antioxidant potential of the extracted oils. Therefore, the results suggest the mixed use of acetone and hexane to obtain oil comparable to that extracted by cold pressing.展开更多
The anti-tumor effect of Adansonia digitata on Ehrlich ascites carcinoma cells (EAC) is still novel talk. This study is focusing on the role of the extracts of seeds and the fruit pulp of Adansonia on the antioxidants...The anti-tumor effect of Adansonia digitata on Ehrlich ascites carcinoma cells (EAC) is still novel talk. This study is focusing on the role of the extracts of seeds and the fruit pulp of Adansonia on the antioxidants activity and the molecular changes of pro-apoptic and anti-apoptic genes expression before and after the treatment of EAC cells bearing mice. Adult female BALB/C mice were used in this study;subgrouped randomly into four groups: control group (non-tumorized);EAC tumorized group, mice was i.p. inoculated with 2.5 × 106 of EAC cells;EAC+ extract of seeds group, tumorized mice was inoculated with 2.5 × 106 of EAC cells and i.p. administered with the extract of Adansonia seeds (300 mg/kg b. wt.);EAC+ fruit pulp group, tumorized mice was inoculated with 2.5 × 106 of EAC cells and i.p. administered with the extract of Adansonia fruit pulp (300 mg/kg b. wt.). The antioxidant enzymes were inhibited in EAC cells and in ascetic fluid of tumorized mice. Also the oxidative stress was increased significantly in EAC cells bearing mice. The liver was affected with the transplantation of EAC cells as reflected by the imbalance in the antioxidants and oxidants in the EAC cells bearing mice. Moreover, the molecular changes in p53 and B-cell lymphoma (Bcl-2) genes expression were recorded in EAC cells bearing mice. The extracts of adansonia have a promising role as antioxidant action due to their antioxidant effect as they ameliorate the imbalance in antioxidants and oxidants balance. The plant extract has anti-apoptosis role by restoring the P53 and Bcl-2 genes expression. Also the plant has antitumor action as they restore tumor markers levels such as α-l-fucosidase and arginase to the normal levels.展开更多
The objective of this study was to determine the polyphenol, flavonoid and tannin content and the antioxidant power of methanolic extracts from the different parts of the Senegalese baobab fruit. Phytochemical screeni...The objective of this study was to determine the polyphenol, flavonoid and tannin content and the antioxidant power of methanolic extracts from the different parts of the Senegalese baobab fruit. Phytochemical screening revealed the presence of saponosides, tannins, alkaloids, sterols, flavonoids, coumarins and total sugars in all extracts from the fruit parts. </span><span style="font-family:""><span style="font-family:Verdana;">The total polyphe</span><span style="font-family:Verdana;">nol content was determined by the folin-ciocalteu method. This method is based on the quantification of the total concentration of hydroxyl groups present in the extract. In an alkaline medium, the reagent of folin-ciocalteu, oxidizes the phenols to ion phenolates and partially reduces its hetero-polyacids</span><span style="font-family:Verdana;">, hence the formation of a blue complex. The absorbance is read at 765 nm against</span><span style="font-family:Verdana;"> a control. The Flavonoids vere determined using </span><span style="font-family:Verdana;">aluminum trichloride and sodium hydroxide. Aluminum trichloride forms a yellow complex with flavonoids and sodium hydroxyde forms a pink complex absorbing in the visible range at 510 nm.</span></span><span style="font-family:""> </span><span style="font-family:Verdana;">The alternative colorimetric method based on reactions with vanillin in an acidic medium made it possible to determine the tannin content. Absorbances were measured at 500 nm.</span><span style="font-family:Verdana;"> The results show that the methanolic extracts of the fibers and shell have very high polyphenol, flavonoid and tannin contents. In fact, the polyphenol contents of the fiber (159.00 ± 0.93 μg EAG/mg extract) and shell (155.39 ± 0.89 μg EAG/mg extract) were much higher than those of the pulp (27.21 ± 0.26 μg EAG/mg extract) and seeds (18.36 ± 0.07 μg EAG/mg extract). In addition, the flavonoid contents of the fibers (97.64 ± 0.40 μg EQ/mg) and of the shell (86.18 ± 0.46 μg EQ/mg) were higher than those of the seeds (12.82 ± 0.04 μg EQ/mg) and pulp (5.66 ± 0.18 μg EQ/mg). The tannin contents of the fibers (256.65 ± 1.45 μg EC/mg) and of the shell (196.05 ± 25 μg EC/mg) are higher than those of the pulp (103.09 ± 0.62 μg EC/mg extract) and seeds (1.09 ± 0.04 μg EC/mg extract). The antioxidant activity of extracts from different parts of the baobab fruit has Also been achieved using two different methods (DPPH and FRAP). The trapping capacity of the DPPH radical is very advantageous for the fibers (IC50 = 2.27 μg/mL) and the shell (IC50 = 1.52 μg/mL). The FRAP test has shown that the extracts from the shell (18.47 μg/mL) and fibers (20.00 μg/mL) have a greater iron reduction capacity than that of the standard ascorbic acid (45.64 μg/mL).展开更多
Besides（-）-epicatechin,epicatechin-（4β-8 ）-epicatechin（procyanidin B2）,epicatechin-（4β-6 ）-epicatechin （procyanidin B5）,epicatechin-（4β-8,2β-O-7）-epicatechin（proanthocyanidin A2） and epicatechin- （4...Besides（-）-epicatechin,epicatechin-（4β-8 ）-epicatechin（procyanidin B2）,epicatechin-（4β-6 ）-epicatechin （procyanidin B5）,epicatechin-（4β-8,2β-O-7）-epicatechin（proanthocyanidin A2） and epicatechin- （4β-8）-epicatechin-（4β-8）-epicatechin（procyanidin C1）,which were isolated before from Adansonia digitata, in this work an A-type proanthocyanidin trimer,i.e.epicatechin-（4β-8）-epicatechin-（4β-8,2β-0-7）- epicatechin,tetrameric procyanidin D1,i.e.epicatechin-（4β-8）-epicatechin-（4β-8）-epicatechin-（4β-8）- epicatechin and a polymeric compound were isolated from the pericarp（fruit wall） of the fruits for the first time from this plant.The antioxidant activity of different fractions and pure compounds was experimentally evaluated in the DPPH<sup>-</sup> assay.The ethyl acetate fraction,and most of the isolated compounds displayed a high activity(IC<sub>50</sub> 2.40-9.60μg/ml) compared with the reference antioxidant Trolox(IC<sub>50</sub> 12.18μg/ml) as a standard.展开更多
The origin,introduction,cultivation,active ingredients,use and advances in propagation methods of Adansonia digitata are reviewed in this paper,with focus on the rapid propagation techniques of plant tissues,to provid...The origin,introduction,cultivation,active ingredients,use and advances in propagation methods of Adansonia digitata are reviewed in this paper,with focus on the rapid propagation techniques of plant tissues,to provide theoretical basis for seedling breeding and promotion of A.digitata.展开更多
Objective: To investigate the effect of different fractions from the aqueous methanolic root bark of Adansonia digitata (Linn) on the estrous cycle of female Wistar rats and to elucidate their phytochemical content. M...Objective: To investigate the effect of different fractions from the aqueous methanolic root bark of Adansonia digitata (Linn) on the estrous cycle of female Wistar rats and to elucidate their phytochemical content. Methods: Crude aqueous methanol extract of Adansonia digitata (Linn) root bark was fractionated by column chromatography using various ratios of hexane, acetyl acetate, methanol and distilled water. Fractions with similar trails on a thin layer chromatography were pooled into four fractions. Each fraction was then screened for its phytochemical content. Forty mature Wistar female rats weighing (135±28) g with regular estrous cycles were given each fraction at two dose levels while a control group received distilled water of equal volume. Treatments were given per os for 15 days starting from the day of estrus. Vaginal smears were observed daily for the duration of each cycle, frequency of occurrence of each stage of the estrous cycle and number of cycles for 15 days before and during treatment. At the end of treatment, rats were humanely sacrificed. Ovaries and uteri were weighed and processed for histopathology. Results: Hexane acetyl acetate and acetyl acetate methanol fractions at 150 mg/kg body weight and 300 mg/kg body weight prolonged the occurrence of proestrus and diestrus and produced atretic cyst-like ovaries at 300 mg/kg body weight. Acetyl acetate methanol treated rats also had low relative ovarian weights when compared to the control. Conclusions: Sterols and triterpenes in hexane acetyl acetate and acetyl acetate methanol fractions of the root bark may be responsible for the disruption of estrous cycle in female Wistar rats and may have a contraceptive effect.展开更多
Objective:To evaluate the effects of aqueous leaf extract of Adansonia(A.)digitata L on dexamethasone-induced testicular damage in male Wistar rats.Methods:Twenty adult male Wistar rats weighing 170-190 g were divided...Objective:To evaluate the effects of aqueous leaf extract of Adansonia(A.)digitata L on dexamethasone-induced testicular damage in male Wistar rats.Methods:Twenty adult male Wistar rats weighing 170-190 g were divided into four groups.GroupⅠreceived 0.5 mL of phosphate buffer orally for 28 days and served as the normal control group;groupⅡreceived 10 mg/kg of dexamethasone(a synthetic glucocorticoid)intraperitoneally for 7 days and 0.5 mL of phosphate buffer orally for 21 days,groupⅢreceived 10 mg/kg of dexamethasone for 7 days and 800 mg/kg of A.digitata extract orally for 21 days;groupⅣreceived 10 mg/kg of dexamethasone for 7 days and 300 mg/kg of vitamin-E orally for 21 days.Dexamethasone was administered intra-peritoneally for 7 days and all administration lasted for 28 days.The rats were sacrificed by anesthesia with diethyl ether and the testes of each animal were harvested.The testis was homogenized in 0.25 M sucrose at 4℃for biochemical and histological analyses.Results:Administration of dexamethasone significantly decreased body weight,glucose-6-phosphate dehydrogenase(G6PDH),lactate dehydrogenase(LDH),superoxide dismutase(SOD),and glutathione peroxidase(GPx)(P<0.05),and significantly increased malondialdehyde(MDA)activities(P<0.05).The degeneration in the population of spermatogonia and vacuolation and abnormal widening of the interstitial spaces were observed in the rats treated with dexamethasone.However,administration of A.digitata significantly increased SOD,GPx,G6PDH,and LDH levels,significantly decreased MDA activities and improved the histoarchitecture of the testis(P<0.05).Conclusions:A.digitata may have an ameliorative effect on dexamethasone-induced testicular damage in Wistar rats because of its anti-inflammatory and antioxidant properties.展开更多
The evolution of some quality markers of baobab (Adansonia digitata L.) was studied. Baobab oil extracted by pressing was packaged in 30 mL non-amber glass bottles and stored at different temperatures: 20°C, 30&#...The evolution of some quality markers of baobab (Adansonia digitata L.) was studied. Baobab oil extracted by pressing was packaged in 30 mL non-amber glass bottles and stored at different temperatures: 20°C, 30°C and 45°C. The physicochemical parameters (acid, peroxide, iodine, saponification, refraction and color indices) were determined during three months’ storage. A significant 5% increase in acid, peroxide and saponification value was observed over time. However, the iodine value decreased. The yellowing and refractive index remained stable while storage at 45°C caused the most significant changes in chemical parameters. The results obtained show that oils stored at 20°C had the lowest acid (2.42 ± 0.26 mg KOH/g) and peroxide (0.82 ± 0.25 mEqO<sub>2</sub>/Kg) value after three months of follow-up.展开更多
Background:The goal of the current study was to identify the potential therapeutic targets by analyzing the production of oxidants and their effects on the antioxidant system of Setaria digitata,a filarial worm that w...Background:The goal of the current study was to identify the potential therapeutic targets by analyzing the production of oxidants and their effects on the antioxidant system of Setaria digitata,a filarial worm that was collected from the peritoneal cavity of recently butchered calves.Methods:S.digitata was collected and the mitochondria-like particles(MLP),somatic isolates and the post-nuclear fraction were prepared for investigation.The radical intensity and the antioxidant enzyme activity in these fractions were evaluated.The effect of diethylcarbamazine(DEC),Q0,omeprazole,and L-methyl arginine on NOS production in the worm was also evaluated.Results:Experiments showed that coelomic fluid and whole worm showed significant levels of Nitric oxide compared to other fractions.The cytosol of the worm showed higher NOS activity than MLP.Superoxide dismutase and nitric oxide(NO)levels were found to be higher in MLP fraction of the worm.It was also found that DEC did not have any effect on the prevention of NO,while Q0,omeprazole,and L-methyl arginine treatment showed a decrease in NO production significantly.Conclusion:The results showed that by using the existing drugs we could reduce the propagation of S.digitata by inhibiting the production of NO.展开更多
Inert surrogates can avoid husbandry and adaptation problems of live vegetation in laboratories. Surrogates are generally used for experiments on vegetation-hydrodynamics interactions, but it is unclear how well they ...Inert surrogates can avoid husbandry and adaptation problems of live vegetation in laboratories. Surrogates are generally used for experiments on vegetation-hydrodynamics interactions, but it is unclear how well they replicate field conditions. Here, surrogates for the brown macroalgae Laminaria digitata were developed to reproduce its hydraulic roughness. Plant shape, stiffness and buoyancy of L. digitata were evaluated and compared to the properties of inert materials. Different surrogate materials and shapes were exposed to unidirectional flow. It is concluded that buoyancy is an important factor in low flow conditions and a basic shape might be sufficient to model complex shaped plants resulting in the same streamlined shape.展开更多
The baobab, Adansonia digitata L., plays an important role in the economy of local populations. Nowadays, baobab seed oil is highly prized for its many cosmetic and therapeutic applications, and for its composition of...The baobab, Adansonia digitata L., plays an important role in the economy of local populations. Nowadays, baobab seed oil is highly prized for its many cosmetic and therapeutic applications, and for its composition of unsaturated fatty acids, sterols, and tocopherols. However, it undergoes numerous reactions during production, processing, transport, and storage, leading to undesirable products that make it unstable. The aim of this study was to provide local processors with innovative solutions for the treatment of unrefined vegetable oils. To this end, an experimental device for filtering crude oil on activated carbon made from fruit capsules was designed. The results obtained after the treatment show a significant decrease at (p < 5%) in acid value (1.62 to 0.58 mg KOH/g), peroxide value (4.40a to 0.50c mEqO<sub>2</sub>/Kg), chlorophyll concentration (1.81 to 0.50 mg/Kg) and primary and secondary oxidation products. According to these results, activated carbon’s adsorptive power eliminates oxidation products and certain pro-oxidants such as chlorophyll, resulting in a cleaner, more stable and better storable oil.展开更多
文摘The aim of the present study was to evaluate the effects of baobab seed washing and origin on the chemical composition of the oil extracted by pressing. Six (6) oil samples were obtained from seeds of three (3) geographical origins. The identification and quantification of fatty acids and the polyphenolic profile were carried out by GC-MS and HPLC-UV, respectively. Analysis of fatty acid methyl esters allowed the identification and quantification of 18 fatty acids. Oils from unwashed seeds were richer in palmitic (C16:0), stearic (C18:0), oleic (C18:1) and arachidic (C20:0) acids. In addition, HPLC-UV analysis at 279 nm shows that oils from unwashed seeds are richer in tyrosol, hydroxytyrosol and caffeic acid. With regard to the polyphenolic profile, gallic acid and quercetin were not detected in these baobab oils. Principal component analysis of fatty acid and phenolic compound content showed that oils from unwashed seeds would best preserve their chemical and associated potential bioactive characteristics.
基金Supported by University Grant Commission,New Delhi,India[Grant No.F.14-2(SC)/2010(SA-Ⅲ)]
文摘Adansonia digitata L.(Malvaceae) is commonly known as baobab tree native to Africa.Baobab is a multi-purpose tree which offers protection and provides food,clothing and medicine as well as raw material for many useful items.The fruit pulp,seeds,leaves,flowers.roots,and bark of baobab are edible and they have been studied by scientists for their useful properties.The fruit pulp have very high vitamin C.calcium,phosphorus,carbohydrates,fibers,potassium,proteins and lipids content,which can be used in seasoning as an appetizer and also make juices.Seeds contain appreciable quantities of phosphorus,magnesium,zinc,sodium,iron,manganese,whereas they have high levels of lysine,thiamine,calcium and iron.Baobab has numerous biological properties including antimicrobial,anti-malarial,diarrhoea,anaemia,asthma,antiviral,anti-oxidant and anti-inflammatory activities amongst others.Phytochemical investigation revealed the presence of flavonoids.phytosterols.amino acids,fatty acids,vitamins and minerals.The review summarizes the information on various aspects of traditional information,taxonomic description,medicinal properties and importantly nutritional value.
基金supported by a grant(SIDA/2006/BT/04) awarded by National Science Foundation of Sri Lanka
文摘Objective:To clone,express and purify a putative parasitic nematode specific protein of Setaria digitata(S.digitata),filarial nematode that infects livestock and cause significant economic losses in Far East and Asia to he used for structural and functional analyses.Methods:To characterize uneharacterized gene of,S.digitata(SDUG),the herterologous expression of SDUG was carried out in the pET[cloned into pET45b(+)]expression system initially and co-expression of SDUC using chaperoiie plasmids pG-KJE8,pGro 7,pKJE7,pG-Tf2 and pTf16 containing chapcrone proteins of dnaK-dnaJ-grpE-groES-gro-E,groES-groEL,dnaK-dnaJ-grpE,groES-groEL-tig,and tig respectively,was carried out subsequently.Results:Expression of SDUG was seen when Escherichia coli strain BI.21(DE3)is used,while concentrating protein largely into the insoluble fraction.The co-expression of SDUG using chaperoiie plasmid mediated system indicated a significant increase of the protein in the soluble fraction.Of the chaperon plasniid sets,the highest amount of recombinant SDUP in the soluble fraction was seen when pGro7 was used in the presence of2 mg/mL L-arabinosc and 0.6M IPTG concentration in the culture medium and for 3 h of incubation at the temperature of 28℃.Recombinant SDUG was purified both from soluble and insoluble fractions using Ni affinity chromatography.SDS-PAGE and western blot analyses of these proteins revealed a single band having expected size of^24 kDa.Conclusions:SDUG seems to be more aggregate-prone and hydrophobic in nature and such protein can make soluble by correct selecting the inducer concentrations and induction temperature and its duration.
文摘In this study, the effect of extraction processes on the physicochemical characteristics and antioxidant potential of baobab (Adansonia digitata L.) seed oil was evaluated. The oils were extracted, on the one hand, by cold pressing, and on the other hand, with three types of organic solvents (acetone, chloroform, n-hexane). The recorded results indicated that the extraction yield of baobab oil was significantly impacted by both the extraction method and the polarity of the solvent used. In addition, chloroform provides the best extraction yield (40.12 ± 0.607). However, extraction by cold pressure preserves at best the physicochemical and bioactive properties of the extracted oils. Indeed, the pressing oil contains a content of phenolic compounds (0.047 ± 0.0024 mgEAG/g of oil) and a very high radical scavenging activity (DPPH) (31.71% ± 0.61%). For the various oils extracted, the minimum and maximum values were 0.50 and 3.17 mEq?kg-1;56.26 and 99.113 mgI2?100 g-1;1.457 and 1.465;205.37 and 233.587 mgKOH/g respectively for the peroxide, iodine, refractive and saponification values. The color parameters (L*, a* and b*) of the oils also differ depending on the nature of the organic solvent used. Principal component analysis (PCA) and correlation analysis were performed on the physicochemical properties and the antioxidant potential of the extracted oils. Therefore, the results suggest the mixed use of acetone and hexane to obtain oil comparable to that extracted by cold pressing.
文摘The anti-tumor effect of Adansonia digitata on Ehrlich ascites carcinoma cells (EAC) is still novel talk. This study is focusing on the role of the extracts of seeds and the fruit pulp of Adansonia on the antioxidants activity and the molecular changes of pro-apoptic and anti-apoptic genes expression before and after the treatment of EAC cells bearing mice. Adult female BALB/C mice were used in this study;subgrouped randomly into four groups: control group (non-tumorized);EAC tumorized group, mice was i.p. inoculated with 2.5 × 106 of EAC cells;EAC+ extract of seeds group, tumorized mice was inoculated with 2.5 × 106 of EAC cells and i.p. administered with the extract of Adansonia seeds (300 mg/kg b. wt.);EAC+ fruit pulp group, tumorized mice was inoculated with 2.5 × 106 of EAC cells and i.p. administered with the extract of Adansonia fruit pulp (300 mg/kg b. wt.). The antioxidant enzymes were inhibited in EAC cells and in ascetic fluid of tumorized mice. Also the oxidative stress was increased significantly in EAC cells bearing mice. The liver was affected with the transplantation of EAC cells as reflected by the imbalance in the antioxidants and oxidants in the EAC cells bearing mice. Moreover, the molecular changes in p53 and B-cell lymphoma (Bcl-2) genes expression were recorded in EAC cells bearing mice. The extracts of adansonia have a promising role as antioxidant action due to their antioxidant effect as they ameliorate the imbalance in antioxidants and oxidants balance. The plant extract has anti-apoptosis role by restoring the P53 and Bcl-2 genes expression. Also the plant has antitumor action as they restore tumor markers levels such as α-l-fucosidase and arginase to the normal levels.
文摘The objective of this study was to determine the polyphenol, flavonoid and tannin content and the antioxidant power of methanolic extracts from the different parts of the Senegalese baobab fruit. Phytochemical screening revealed the presence of saponosides, tannins, alkaloids, sterols, flavonoids, coumarins and total sugars in all extracts from the fruit parts. </span><span style="font-family:""><span style="font-family:Verdana;">The total polyphe</span><span style="font-family:Verdana;">nol content was determined by the folin-ciocalteu method. This method is based on the quantification of the total concentration of hydroxyl groups present in the extract. In an alkaline medium, the reagent of folin-ciocalteu, oxidizes the phenols to ion phenolates and partially reduces its hetero-polyacids</span><span style="font-family:Verdana;">, hence the formation of a blue complex. The absorbance is read at 765 nm against</span><span style="font-family:Verdana;"> a control. The Flavonoids vere determined using </span><span style="font-family:Verdana;">aluminum trichloride and sodium hydroxide. Aluminum trichloride forms a yellow complex with flavonoids and sodium hydroxyde forms a pink complex absorbing in the visible range at 510 nm.</span></span><span style="font-family:""> </span><span style="font-family:Verdana;">The alternative colorimetric method based on reactions with vanillin in an acidic medium made it possible to determine the tannin content. Absorbances were measured at 500 nm.</span><span style="font-family:Verdana;"> The results show that the methanolic extracts of the fibers and shell have very high polyphenol, flavonoid and tannin contents. In fact, the polyphenol contents of the fiber (159.00 ± 0.93 μg EAG/mg extract) and shell (155.39 ± 0.89 μg EAG/mg extract) were much higher than those of the pulp (27.21 ± 0.26 μg EAG/mg extract) and seeds (18.36 ± 0.07 μg EAG/mg extract). In addition, the flavonoid contents of the fibers (97.64 ± 0.40 μg EQ/mg) and of the shell (86.18 ± 0.46 μg EQ/mg) were higher than those of the seeds (12.82 ± 0.04 μg EQ/mg) and pulp (5.66 ± 0.18 μg EQ/mg). The tannin contents of the fibers (256.65 ± 1.45 μg EC/mg) and of the shell (196.05 ± 25 μg EC/mg) are higher than those of the pulp (103.09 ± 0.62 μg EC/mg extract) and seeds (1.09 ± 0.04 μg EC/mg extract). The antioxidant activity of extracts from different parts of the baobab fruit has Also been achieved using two different methods (DPPH and FRAP). The trapping capacity of the DPPH radical is very advantageous for the fibers (IC50 = 2.27 μg/mL) and the shell (IC50 = 1.52 μg/mL). The FRAP test has shown that the extracts from the shell (18.47 μg/mL) and fibers (20.00 μg/mL) have a greater iron reduction capacity than that of the standard ascorbic acid (45.64 μg/mL).
文摘Besides（-）-epicatechin,epicatechin-（4β-8 ）-epicatechin（procyanidin B2）,epicatechin-（4β-6 ）-epicatechin （procyanidin B5）,epicatechin-（4β-8,2β-O-7）-epicatechin（proanthocyanidin A2） and epicatechin- （4β-8）-epicatechin-（4β-8）-epicatechin（procyanidin C1）,which were isolated before from Adansonia digitata, in this work an A-type proanthocyanidin trimer,i.e.epicatechin-（4β-8）-epicatechin-（4β-8,2β-0-7）- epicatechin,tetrameric procyanidin D1,i.e.epicatechin-（4β-8）-epicatechin-（4β-8）-epicatechin-（4β-8）- epicatechin and a polymeric compound were isolated from the pericarp（fruit wall） of the fruits for the first time from this plant.The antioxidant activity of different fractions and pure compounds was experimentally evaluated in the DPPH<sup>-</sup> assay.The ethyl acetate fraction,and most of the isolated compounds displayed a high activity(IC<sub>50</sub> 2.40-9.60μg/ml) compared with the reference antioxidant Trolox(IC<sub>50</sub> 12.18μg/ml) as a standard.
基金Supported by Natural Science Foundation of Hainan Province(317205)Key Research and Development Project of Hainan Province(ZDYF2016065)
文摘The origin,introduction,cultivation,active ingredients,use and advances in propagation methods of Adansonia digitata are reviewed in this paper,with focus on the rapid propagation techniques of plant tissues,to provide theoretical basis for seedling breeding and promotion of A.digitata.
文摘Objective: To investigate the effect of different fractions from the aqueous methanolic root bark of Adansonia digitata (Linn) on the estrous cycle of female Wistar rats and to elucidate their phytochemical content. Methods: Crude aqueous methanol extract of Adansonia digitata (Linn) root bark was fractionated by column chromatography using various ratios of hexane, acetyl acetate, methanol and distilled water. Fractions with similar trails on a thin layer chromatography were pooled into four fractions. Each fraction was then screened for its phytochemical content. Forty mature Wistar female rats weighing (135±28) g with regular estrous cycles were given each fraction at two dose levels while a control group received distilled water of equal volume. Treatments were given per os for 15 days starting from the day of estrus. Vaginal smears were observed daily for the duration of each cycle, frequency of occurrence of each stage of the estrous cycle and number of cycles for 15 days before and during treatment. At the end of treatment, rats were humanely sacrificed. Ovaries and uteri were weighed and processed for histopathology. Results: Hexane acetyl acetate and acetyl acetate methanol fractions at 150 mg/kg body weight and 300 mg/kg body weight prolonged the occurrence of proestrus and diestrus and produced atretic cyst-like ovaries at 300 mg/kg body weight. Acetyl acetate methanol treated rats also had low relative ovarian weights when compared to the control. Conclusions: Sterols and triterpenes in hexane acetyl acetate and acetyl acetate methanol fractions of the root bark may be responsible for the disruption of estrous cycle in female Wistar rats and may have a contraceptive effect.
文摘Objective:To evaluate the effects of aqueous leaf extract of Adansonia(A.)digitata L on dexamethasone-induced testicular damage in male Wistar rats.Methods:Twenty adult male Wistar rats weighing 170-190 g were divided into four groups.GroupⅠreceived 0.5 mL of phosphate buffer orally for 28 days and served as the normal control group;groupⅡreceived 10 mg/kg of dexamethasone(a synthetic glucocorticoid)intraperitoneally for 7 days and 0.5 mL of phosphate buffer orally for 21 days,groupⅢreceived 10 mg/kg of dexamethasone for 7 days and 800 mg/kg of A.digitata extract orally for 21 days;groupⅣreceived 10 mg/kg of dexamethasone for 7 days and 300 mg/kg of vitamin-E orally for 21 days.Dexamethasone was administered intra-peritoneally for 7 days and all administration lasted for 28 days.The rats were sacrificed by anesthesia with diethyl ether and the testes of each animal were harvested.The testis was homogenized in 0.25 M sucrose at 4℃for biochemical and histological analyses.Results:Administration of dexamethasone significantly decreased body weight,glucose-6-phosphate dehydrogenase(G6PDH),lactate dehydrogenase(LDH),superoxide dismutase(SOD),and glutathione peroxidase(GPx)(P<0.05),and significantly increased malondialdehyde(MDA)activities(P<0.05).The degeneration in the population of spermatogonia and vacuolation and abnormal widening of the interstitial spaces were observed in the rats treated with dexamethasone.However,administration of A.digitata significantly increased SOD,GPx,G6PDH,and LDH levels,significantly decreased MDA activities and improved the histoarchitecture of the testis(P<0.05).Conclusions:A.digitata may have an ameliorative effect on dexamethasone-induced testicular damage in Wistar rats because of its anti-inflammatory and antioxidant properties.
文摘The evolution of some quality markers of baobab (Adansonia digitata L.) was studied. Baobab oil extracted by pressing was packaged in 30 mL non-amber glass bottles and stored at different temperatures: 20°C, 30°C and 45°C. The physicochemical parameters (acid, peroxide, iodine, saponification, refraction and color indices) were determined during three months’ storage. A significant 5% increase in acid, peroxide and saponification value was observed over time. However, the iodine value decreased. The yellowing and refractive index remained stable while storage at 45°C caused the most significant changes in chemical parameters. The results obtained show that oils stored at 20°C had the lowest acid (2.42 ± 0.26 mg KOH/g) and peroxide (0.82 ± 0.25 mEqO<sub>2</sub>/Kg) value after three months of follow-up.
文摘Background:The goal of the current study was to identify the potential therapeutic targets by analyzing the production of oxidants and their effects on the antioxidant system of Setaria digitata,a filarial worm that was collected from the peritoneal cavity of recently butchered calves.Methods:S.digitata was collected and the mitochondria-like particles(MLP),somatic isolates and the post-nuclear fraction were prepared for investigation.The radical intensity and the antioxidant enzyme activity in these fractions were evaluated.The effect of diethylcarbamazine(DEC),Q0,omeprazole,and L-methyl arginine on NOS production in the worm was also evaluated.Results:Experiments showed that coelomic fluid and whole worm showed significant levels of Nitric oxide compared to other fractions.The cytosol of the worm showed higher NOS activity than MLP.Superoxide dismutase and nitric oxide(NO)levels were found to be higher in MLP fraction of the worm.It was also found that DEC did not have any effect on the prevention of NO,while Q0,omeprazole,and L-methyl arginine treatment showed a decrease in NO production significantly.Conclusion:The results showed that by using the existing drugs we could reduce the propagation of S.digitata by inhibiting the production of NO.
基金supported by the European Community’s 7th Framework Programme through the grant to the budget of the Integrated Infrastructure Initiative HYDRALAB-IV, Contract No. 261520
文摘Inert surrogates can avoid husbandry and adaptation problems of live vegetation in laboratories. Surrogates are generally used for experiments on vegetation-hydrodynamics interactions, but it is unclear how well they replicate field conditions. Here, surrogates for the brown macroalgae Laminaria digitata were developed to reproduce its hydraulic roughness. Plant shape, stiffness and buoyancy of L. digitata were evaluated and compared to the properties of inert materials. Different surrogate materials and shapes were exposed to unidirectional flow. It is concluded that buoyancy is an important factor in low flow conditions and a basic shape might be sufficient to model complex shaped plants resulting in the same streamlined shape.
文摘The baobab, Adansonia digitata L., plays an important role in the economy of local populations. Nowadays, baobab seed oil is highly prized for its many cosmetic and therapeutic applications, and for its composition of unsaturated fatty acids, sterols, and tocopherols. However, it undergoes numerous reactions during production, processing, transport, and storage, leading to undesirable products that make it unstable. The aim of this study was to provide local processors with innovative solutions for the treatment of unrefined vegetable oils. To this end, an experimental device for filtering crude oil on activated carbon made from fruit capsules was designed. The results obtained after the treatment show a significant decrease at (p < 5%) in acid value (1.62 to 0.58 mg KOH/g), peroxide value (4.40a to 0.50c mEqO<sub>2</sub>/Kg), chlorophyll concentration (1.81 to 0.50 mg/Kg) and primary and secondary oxidation products. According to these results, activated carbon’s adsorptive power eliminates oxidation products and certain pro-oxidants such as chlorophyll, resulting in a cleaner, more stable and better storable oil.
