The differential hybridization technique hasbeen widely used to identify genes that are dif-ferentially expressed.However,this approachhas several drawbacks.First,the screeningprocedures are rather labor-intensive and...The differential hybridization technique hasbeen widely used to identify genes that are dif-ferentially expressed.However,this approachhas several drawbacks.First,the screeningprocedures are rather labor-intensive and time-consuming.Second,the amount of phageDNAs transferred onto the two filters may notbe equivalent,which leads to an inaccurate se-lection of a positive clone.Third,isolation ofphage DNA is slow and cumbersome.Here,aPCR based differential screening method that展开更多
cDNA libraries were constructed from the leaves of a rice (Oryza sativa L.) salt tolerancevariety Tesan抋i 2 growing in solutions with 150 mmol/L NaCl for 3 h or without salt stress. Three salt-responsive cDNA clones,...cDNA libraries were constructed from the leaves of a rice (Oryza sativa L.) salt tolerancevariety Tesan抋i 2 growing in solutions with 150 mmol/L NaCl for 3 h or without salt stress. Three salt-responsive cDNA clones, Ts1, Ts2 and Ts3 were isolated by differential screening. Northern blottinganalysis showed that the transcription levels of Ts1 and Ts2 increased within 3 h salt stress and kept onincreasing within 24 h, while the transcription level of Ts3 reached its peak within 3 h. Sequence analysisindicated that there were no homologies between the three cDNA clones and any known gene. The threecDNA clones were mapped using a doubled haploid (DH) population derived from an indica variety ZYQ8,which was a salt tolerance parent of Tesan抋i 2, with a japonica variety JX17. Ts1, Ts2 and Ts3 werelocated on chromosomes 1, 3 and 7, respectively. It was noted that Ts1, Ts2, and Ts3 were in or near theregions of major or minor salt tolerance quantitative trait loci (QTLs), which were mapped in the same DHpopulation in a parallel study.展开更多
Dietary factors play a crucial role in irritable bowel syndrome(IBS)pathogenesis.Therefore,the dietary contraindications for patients with IBS require further supplementation.Recent investigations have revealed that g...Dietary factors play a crucial role in irritable bowel syndrome(IBS)pathogenesis.Therefore,the dietary contraindications for patients with IBS require further supplementation.Recent investigations have revealed that ginger consumption may pose a risk of aggravating the symptoms and incidence of IBS;however,the specific mechanism remains unknown.In this study,we developed experimental IBS and intestinal organoid differentiation screening models to elucidate the mechanisms underlying the gingermediated exacerbation of IBS symptoms.Subsequently,we used a knockout approach combined with click chemistry as well as virus infection to identify the toxic components of ginger and the target mechanism.Our results showed that a daily intake of 90 to 300 mg/kg ginger(equivalent to a human daily dose of O.6 to 2 g per person)may pose a risk of exacerbating IBS symptoms.Furthermore,a component derived from 6-gingerol(ginger's main ingredient)through in vivo gastric acid and heat processing inhibited the formation of the elF3 transcription initiation complex by covalently binding to the Cys^(58)site of elF3A,a key factor regulating intestinal crypt stem celldifferentiation,further reducing the goblet cellnumber and related mucus layer thickness and increasing lipopolysaccharide infiltration and low-grade inflammation in the ileum crypts,thereby exacerbating the symptoms of IBS in mice.Our study suggests that dietary ginger aggravates IBS and provides safety evaluation methods for the proper use of foods in specific populations.展开更多
文摘The differential hybridization technique hasbeen widely used to identify genes that are dif-ferentially expressed.However,this approachhas several drawbacks.First,the screeningprocedures are rather labor-intensive and time-consuming.Second,the amount of phageDNAs transferred onto the two filters may notbe equivalent,which leads to an inaccurate se-lection of a positive clone.Third,isolation ofphage DNA is slow and cumbersome.Here,aPCR based differential screening method that
文摘cDNA libraries were constructed from the leaves of a rice (Oryza sativa L.) salt tolerancevariety Tesan抋i 2 growing in solutions with 150 mmol/L NaCl for 3 h or without salt stress. Three salt-responsive cDNA clones, Ts1, Ts2 and Ts3 were isolated by differential screening. Northern blottinganalysis showed that the transcription levels of Ts1 and Ts2 increased within 3 h salt stress and kept onincreasing within 24 h, while the transcription level of Ts3 reached its peak within 3 h. Sequence analysisindicated that there were no homologies between the three cDNA clones and any known gene. The threecDNA clones were mapped using a doubled haploid (DH) population derived from an indica variety ZYQ8,which was a salt tolerance parent of Tesan抋i 2, with a japonica variety JX17. Ts1, Ts2 and Ts3 werelocated on chromosomes 1, 3 and 7, respectively. It was noted that Ts1, Ts2, and Ts3 were in or near theregions of major or minor salt tolerance quantitative trait loci (QTLs), which were mapped in the same DHpopulation in a parallel study.
基金supported by the National Natural Science Foundation of China(grant numbers 82125037,8197-3498,82104508,and 81774283)Science Foundation for Distinguished Young Scholars of Jiangsu Province(BK20231528)+5 种基金the National Key Research and Development Program of China(2023YFC2308200)Key R&D Program of Jiangsu Province(grant number BE2022815)Jiangsu Provincial Medical Innovation Center(grant number CXZX202225)Young Elite Scientists Sponsorship Program of the China Association for Science and Technology(grant number 2021-QNRC2-B17)333 high-level talents project of Jiangsu Province for Y.Y.(BRA202202)Suzhou GuSu Medical Talent Project(GSWS2022100).
文摘Dietary factors play a crucial role in irritable bowel syndrome(IBS)pathogenesis.Therefore,the dietary contraindications for patients with IBS require further supplementation.Recent investigations have revealed that ginger consumption may pose a risk of aggravating the symptoms and incidence of IBS;however,the specific mechanism remains unknown.In this study,we developed experimental IBS and intestinal organoid differentiation screening models to elucidate the mechanisms underlying the gingermediated exacerbation of IBS symptoms.Subsequently,we used a knockout approach combined with click chemistry as well as virus infection to identify the toxic components of ginger and the target mechanism.Our results showed that a daily intake of 90 to 300 mg/kg ginger(equivalent to a human daily dose of O.6 to 2 g per person)may pose a risk of exacerbating IBS symptoms.Furthermore,a component derived from 6-gingerol(ginger's main ingredient)through in vivo gastric acid and heat processing inhibited the formation of the elF3 transcription initiation complex by covalently binding to the Cys^(58)site of elF3A,a key factor regulating intestinal crypt stem celldifferentiation,further reducing the goblet cellnumber and related mucus layer thickness and increasing lipopolysaccharide infiltration and low-grade inflammation in the ileum crypts,thereby exacerbating the symptoms of IBS in mice.Our study suggests that dietary ginger aggravates IBS and provides safety evaluation methods for the proper use of foods in specific populations.
