The treatment of hepatocellular carcinoma(HCC)faces challenges of low response rates to targeted drugs and immune checkpoint inhibitors,which are influenced by complicated microenvironment of HCC.In this study,the com...The treatment of hepatocellular carcinoma(HCC)faces challenges of low response rates to targeted drugs and immune checkpoint inhibitors,which are influenced by complicated microenvironment of HCC.In this study,the complex tumor microenvironment was identified by using tissue microarray(TMA),spatial transcriptomes and single-cell sequencing.High expression of CC chemokine receptor 7(CCR7)in tumor cells predicted lower Overall Survival(OS).Conversely,CRISPR-Cas9-mediated knockout of CCR7 enhanced the sensitivity of HCC to sorafenib in preclinical experiments,resulting from the inhibition of epithelial-mesenchymal transition through the AKT and ERK signaling pathways.展开更多
Maize （Zea raays L.） is one of the most important crops because of the remarkable properties of its hybrid, which is responsible for the high commercial value of hybrid maize. The genetic basis of heterosis （hybrid...Maize （Zea raays L.） is one of the most important crops because of the remarkable properties of its hybrid, which is responsible for the high commercial value of hybrid maize. The genetic basis of heterosis （hybrid vigor） is not well understood. A differential display technique was performed to identify genes with differential expression across twelve maize inbred lines and thirty-three hybrids during ear development. An incomplete diallel design was used to investigate the relationship between the global framework of differential gene expression and heterosis. It was found that the genes belonging to MONO pattern （i.e., genes expressed in both parental lines and in hybrid） was the highest in percentage among the total five patterns and illustrated that the properties of differentially expressed genes are not entirely responsible for heterosis. Furthermore,a larger number of differentially expressed genes in hybrid, which serves as a major reservoir for generating novel phenotypes that exhibit heterosis of certain agronomic traits during early development and differentiation of maize ear. Moreover, there were some silent genesin hybrids that are responsible for the arrest or abortion of spikelets and for the increase in kernels weight.展开更多
基金funded by the National Natural Science Foundation of China[31971111,U24A20729,31471147 from Chunmin Liang,82373313 from Shuangjian Qiu,82090053 from Jieyi Shi,32270886 from Zuoyun Wang).
文摘The treatment of hepatocellular carcinoma(HCC)faces challenges of low response rates to targeted drugs and immune checkpoint inhibitors,which are influenced by complicated microenvironment of HCC.In this study,the complex tumor microenvironment was identified by using tissue microarray(TMA),spatial transcriptomes and single-cell sequencing.High expression of CC chemokine receptor 7(CCR7)in tumor cells predicted lower Overall Survival(OS).Conversely,CRISPR-Cas9-mediated knockout of CCR7 enhanced the sensitivity of HCC to sorafenib in preclinical experiments,resulting from the inhibition of epithelial-mesenchymal transition through the AKT and ERK signaling pathways.
文摘Maize （Zea raays L.） is one of the most important crops because of the remarkable properties of its hybrid, which is responsible for the high commercial value of hybrid maize. The genetic basis of heterosis （hybrid vigor） is not well understood. A differential display technique was performed to identify genes with differential expression across twelve maize inbred lines and thirty-three hybrids during ear development. An incomplete diallel design was used to investigate the relationship between the global framework of differential gene expression and heterosis. It was found that the genes belonging to MONO pattern （i.e., genes expressed in both parental lines and in hybrid） was the highest in percentage among the total five patterns and illustrated that the properties of differentially expressed genes are not entirely responsible for heterosis. Furthermore,a larger number of differentially expressed genes in hybrid, which serves as a major reservoir for generating novel phenotypes that exhibit heterosis of certain agronomic traits during early development and differentiation of maize ear. Moreover, there were some silent genesin hybrids that are responsible for the arrest or abortion of spikelets and for the increase in kernels weight.
