Idesia polycarpa Maxim.var vestita Diels.is a dioecious tree species native to eastern Asia.There are diffi culties associated with distinguishing the sex of the plant at the seedling stage.In order to explore the mec...Idesia polycarpa Maxim.var vestita Diels.is a dioecious tree species native to eastern Asia.There are diffi culties associated with distinguishing the sex of the plant at the seedling stage.In order to explore the mechanism of sex diff erentiation in fl ower development,we conducted the transcriptome profi les of male and female fl owers at early,metaphase and late developmental stages.Approximately 123,335 unigenes with a total length of 83,996 Mb and an average length of 168 bp were assembled.The unigenes were blasted into Nr,Nt,Pfam,KOG/COG,Swiss-prot,KEGG,GO databases.Homology analysis demonstrated that I.polycarpa and black cottonwood had the highest homology with the alignment of 92,871 sequences.This study identifi ed 80 groups of transcription factor families with a total of 1475 unigenes,mainly including MYB,WRKY,AP2 and bHLH transcription factor families.KEGG pathway analysis showed that the expression of numerous plant hormones(cytokinin,gibberellin and ethylene)and fl avonoid biosynthesis pathway were diff erent at various stages of female and male fl ower development.In addition,a number of unigenes associated with fl owering were identifi ed which were key genes associated with photoperiodic,vernalization,thermosensory,gibberellin,and autonomic pathways.The results show that I.polycarpa fl oral organ development was in accordance with the ABCDE model,in which the down-regulation of the B gene family might aff ect stamen fertility in late stages of female fl ower development.qRTPCR experiments validated that the expression patterns of 15 unigenes were consistent with those in RNA-seq results.The results highlight a central role for plant sex identifi cation in seedling production and a sex-determining mechanism for dioecious plants.In addition,the transcriptome data provided a theoretical basis for I.polycarpa genetic diversity analysis and molecular-assisted breeding.展开更多
Coloration is an important phenotypic trait for multiple adaptive functions.It is interesting to fi nd white-eye(AW)and orange-eye(AO)phenotypes in the shrimp Alvinocaris longirostris inhabiting the deep-sea cold seep...Coloration is an important phenotypic trait for multiple adaptive functions.It is interesting to fi nd white-eye(AW)and orange-eye(AO)phenotypes in the shrimp Alvinocaris longirostris inhabiting the deep-sea cold seep and hydrothermal vent areas of the northwestern Pacifi c.By comparative transcriptome analyses,1491 diff erentially expressed genes(DEGs)were identified between AW and AO.Among them,many DEGs were associated with immunity,antioxidation,and detoxifi cation.Two signifi cant enzyme encoding genes,xanthine dehydrogenase,and tryptophan oxidase involved in pigment biosynthesis pathways were up-regulated in AW and AO,respectively,which might be related to the diff erences of white and orange eye phenotypes.Moreover,single nucleotide polymorphism(SNP)calling detected that genotypes of 28 SNP distributing in 14 unigenes were completely diff erent between AW and AO.Particularly,there were three and two non-synonymous mutations in immune genes crustin Pm5 and antimicrobial peptide,respectively.Results indicate that the diff erence in eye color is probably resulted from immune response to variable micro-environmental stressors encountered in the dispersal process of the shrimps,such as symbiotic microbes,pathogens,and toxic substances,and might be genetically fi xed at last.The suggested pathway preliminarily explained the formation mechanism of diff erent eye phenotypes in Alvinocaridid shrimps,providing a basis for further study on adaptive evolution of eyes in deep-sea chemosynthetic faunas.展开更多
BACKGROUND:To investigate the eff ects of early standardized enteral nutrition(EN)on the crosssectional area of erector spine muscle(ESMcsa),plasma growth diff erentiation factor-15(GDF-15),and 28-day mortality of acu...BACKGROUND:To investigate the eff ects of early standardized enteral nutrition(EN)on the crosssectional area of erector spine muscle(ESMcsa),plasma growth diff erentiation factor-15(GDF-15),and 28-day mortality of acute exacerbation of chronic obstructive pulmonary disease(AECOPD)patients with invasive mechanical ventilation(MV).METHODS:A total of 97 AECOPD patients with invasive MV were screened in the ICUs of the First People's Hospital of Lianyungang.The conventional EN group(stage Ⅰ)and early standardized EN group(stage Ⅱ)included 46 and 51 patients,respectively.ESMcsa loss and GDF-15 levels on days 1 and 7 of ICU admission and 28-day survival rates were analyzed.RESULTS:On day 7,the ESMcsa of the early standardized EN group was significantly higher than that of the conventional EN group,while the plasma GDF-15 levels were significantly lower than those in the conventional EN group(ESMcsa:28.426±6.130 cm^(2) vs.25.205±6.127 cm^(2);GDF-15:1661.608±558.820 pg/mL vs.2541.000±634.845 pg/mL;all P<0.001).The 28-day survival rates of the patients in the early standardized EN group and conventional EN group were 80.40%and 73.90%,respectively(P=0.406).CONCLUSION:ESMcsa loss in AECOPD patients with MV was correlated with GDF-15 levels,both of which indicated acute muscular atrophy and skeletal muscle dysfunction.Early standardized EN may prevent acute muscle loss and intensive care unit-acquired weakness(ICU-AW)in AECOPD patients.展开更多
A number of high-temperature processes(e.g.,melt-rock reactions,metasomatism,partial melting)can produce signifi cant Ca isotopic fractionation and heterogeneity in the mantle,but the mechanism for such fractionation ...A number of high-temperature processes(e.g.,melt-rock reactions,metasomatism,partial melting)can produce signifi cant Ca isotopic fractionation and heterogeneity in the mantle,but the mechanism for such fractionation remains obscure.To investigate the eff ect of mantle partial melting on Ca isotopic fractionation,we reported high-precision Ca isotopic compositions of depleted mid-ocean ridge basalts(MORBs)from the East Pacifi c Rise and Ecuador Rift in the northeastern Pacifi c.Theδ44/40 Ca of these MORB samples exhibit a narrow variation from 0.84‰to 0.88‰with an average of 0.85‰±0.03‰,which are similar to those of reported MORBs(0.83‰±0.11‰)and back-arc basin basalts(BABBs,0.80‰±0.08‰)in literature,but are lower than the estimate value for the bulk silicate Earth(BSE,0.94‰±0.05‰).The lowδ44/40 Ca signatures of MORB samples in this study cannot be caused by fractional crystallization,since intermediate-mafi c diff erentiation has been demonstrated having only limited eff ects on Ca isotopic fractionation.Instead,the off set ofδ44/40 Ca between MORBs and the BSE is most likely produced by mantle partial melting.During this process,the light Ca isotopes are preferentially transferred to the melt,while the heavy ones tend to stay in the residue,which is consistent with the fact thatδ44/40 Ca of melt-depleted peridotites increases with partial melting in literature.The behavior of Ca isotopes during mantle partial melting is closely related to the inter-mineral(Cpx and Opx)Ca isotopic fractionation and melting mineral modes.Mantle partial melting is one of the common processes that can induce lowerδ44/40 Ca values in basalts and Ca isotopic heterogeneity in Earth’s mantle.展开更多
The vector transformation and pole reduction from the total-field anomaly are signifi cant for the interpretation.We examined these industry-standard processing procedures in the Fourier domain.We propose a novel iter...The vector transformation and pole reduction from the total-field anomaly are signifi cant for the interpretation.We examined these industry-standard processing procedures in the Fourier domain.We propose a novel iteration algorithm for regional magnetic anomalies transformations to derive the vertical-component data from the total-field measurements with the variation in the core-fi eld direction over the region.Additionally,we use the same algorithm to convert the calculated vertical-component data into the corresponding data at the pole and realize the processing of diff erential reduction to the pole(DRTP).Unlike Arkani-Hamed’s DRTP method,the two types of iterative algorithms have the same forms,and DRTP is realized by implementing this algorithm twice.The synthetic model’s calculation results show that the method has high accuracy,and the fi eld data processing confi rms its practicality.展开更多
In this paper, we present a brief survey on the updated theory of backward stochas-tic Volterra integral equations （BSVIEs, for short）. BSVIEs are a natural generalization of backward stochastic diff erential equati...In this paper, we present a brief survey on the updated theory of backward stochas-tic Volterra integral equations （BSVIEs, for short）. BSVIEs are a natural generalization of backward stochastic diff erential equations （BSDEs, for short）. Some interesting motivations of studying BSVIEs are recalled. With proper solution concepts, it is possible to establish the corresponding well-posedness for BSVIEs. We also survey various comparison theorems for solutions to BSVIEs.展开更多
Haliotis discus hannai is an important marine economic species in China.Its egg color was found to be associated with economic traits,which provides a new idea for breeding.However,the molecular mechanism of the egg-c...Haliotis discus hannai is an important marine economic species in China.Its egg color was found to be associated with economic traits,which provides a new idea for breeding.However,the molecular mechanism of the egg-color formation has not been reported.Thus,the pigment composition and comparative transcriptome analyses of H.discus hannai with green and gray egg color were conducted using high-performance liquid chromatography(HPLC)and RNA-Seq methods.Results show that individuals with green and gray eggs both possess the fucoxanthin.Lutein existed in gray-egged individuals,but not in green-egged individuals.In transcriptome analysis,272310 unigenes were received from 461162 transcripts with a mean length of 985 bp and N50 of 1524 bp,respectively.A total of 185 unigenes were identifi ed as diff erentially expressed genes(DEGs).The DEGs involved in“fl avin-containing compound metabolic process”,“melanosome”,“glutathione metabolism”,and“cytochrome b6f complex”were likely related to the formation of the egg color.Our results provide foundational information for the functional analysis of egg-color related genes and are benefi cial to the selective breeding of H.discus hannai.展开更多
Larix olgensis A.Henry is a fast-growing tree used for aff orestation in northeastern China and has great ecological and economic value.For studying developmental genes in the xylem of this species,we investigated the...Larix olgensis A.Henry is a fast-growing tree used for aff orestation in northeastern China and has great ecological and economic value.For studying developmental genes in the xylem of this species,we investigated the Myb transcription factor family,one of the largest families of transcription factors in plants,which plays an important role in the regulation of lignifi cation in plant secondary walls.By sequencing a L.olgensis cDNA library using the Illumina HiSeq2500 high-throughput sequencing platform,we obtained 58,683 unigene sequences,of which 16,554 unigenes were longer than 1000 bp,accounting for 28.2%of the total database.The alignment of these genes with the GO,COG,KEGG,Swiss-Prot and NR databases resulted in annotated 29,350 unigenes.We obtained a total of 1460 differentially expressed genes,of which 453 were upregulated and 1007 were downregulated at the two developmental stages analyzed.The gene annotations showed a wide range of biological functions and metabolic pathways.The 10 Myb transcription factors that were obtained from the diff erentially expressed genes were analyzed by real-time quantitative PCR(qRT-PCR).The results showed that four Myb transcription factors may be associated with xylem development in L.olgensis.Due to the large genome size of conifers,genomics research on these species has lagged behind that for other plant groups.Our data provide the basis for further studies on xylem development in L.olgensis.展开更多
文摘Idesia polycarpa Maxim.var vestita Diels.is a dioecious tree species native to eastern Asia.There are diffi culties associated with distinguishing the sex of the plant at the seedling stage.In order to explore the mechanism of sex diff erentiation in fl ower development,we conducted the transcriptome profi les of male and female fl owers at early,metaphase and late developmental stages.Approximately 123,335 unigenes with a total length of 83,996 Mb and an average length of 168 bp were assembled.The unigenes were blasted into Nr,Nt,Pfam,KOG/COG,Swiss-prot,KEGG,GO databases.Homology analysis demonstrated that I.polycarpa and black cottonwood had the highest homology with the alignment of 92,871 sequences.This study identifi ed 80 groups of transcription factor families with a total of 1475 unigenes,mainly including MYB,WRKY,AP2 and bHLH transcription factor families.KEGG pathway analysis showed that the expression of numerous plant hormones(cytokinin,gibberellin and ethylene)and fl avonoid biosynthesis pathway were diff erent at various stages of female and male fl ower development.In addition,a number of unigenes associated with fl owering were identifi ed which were key genes associated with photoperiodic,vernalization,thermosensory,gibberellin,and autonomic pathways.The results show that I.polycarpa fl oral organ development was in accordance with the ABCDE model,in which the down-regulation of the B gene family might aff ect stamen fertility in late stages of female fl ower development.qRTPCR experiments validated that the expression patterns of 15 unigenes were consistent with those in RNA-seq results.The results highlight a central role for plant sex identifi cation in seedling production and a sex-determining mechanism for dioecious plants.In addition,the transcriptome data provided a theoretical basis for I.polycarpa genetic diversity analysis and molecular-assisted breeding.
基金Supported by the National Key R&D Program of China(No.2018YFC0310802)the National Natural Science Foundation of China(No.31872215)+2 种基金the Senior User Project of R/V Kexue(No.KEXUE2019GZ02)the Strategic Priority Research Program of the Chinese Academy of Sciences(CAS)(No.XDA22050302)the Key Research Program of Frontier Sciences,CAS(No.QYZDB-SSW-DQC036)。
文摘Coloration is an important phenotypic trait for multiple adaptive functions.It is interesting to fi nd white-eye(AW)and orange-eye(AO)phenotypes in the shrimp Alvinocaris longirostris inhabiting the deep-sea cold seep and hydrothermal vent areas of the northwestern Pacifi c.By comparative transcriptome analyses,1491 diff erentially expressed genes(DEGs)were identified between AW and AO.Among them,many DEGs were associated with immunity,antioxidation,and detoxifi cation.Two signifi cant enzyme encoding genes,xanthine dehydrogenase,and tryptophan oxidase involved in pigment biosynthesis pathways were up-regulated in AW and AO,respectively,which might be related to the diff erences of white and orange eye phenotypes.Moreover,single nucleotide polymorphism(SNP)calling detected that genotypes of 28 SNP distributing in 14 unigenes were completely diff erent between AW and AO.Particularly,there were three and two non-synonymous mutations in immune genes crustin Pm5 and antimicrobial peptide,respectively.Results indicate that the diff erence in eye color is probably resulted from immune response to variable micro-environmental stressors encountered in the dispersal process of the shrimps,such as symbiotic microbes,pathogens,and toxic substances,and might be genetically fi xed at last.The suggested pathway preliminarily explained the formation mechanism of diff erent eye phenotypes in Alvinocaridid shrimps,providing a basis for further study on adaptive evolution of eyes in deep-sea chemosynthetic faunas.
基金funded by the Social Development Project of Jiangsu Provincial Department of Science and Technology(BE2020670)the Social Development Project of Lianyungang Science and Technology(SF2117).
文摘BACKGROUND:To investigate the eff ects of early standardized enteral nutrition(EN)on the crosssectional area of erector spine muscle(ESMcsa),plasma growth diff erentiation factor-15(GDF-15),and 28-day mortality of acute exacerbation of chronic obstructive pulmonary disease(AECOPD)patients with invasive mechanical ventilation(MV).METHODS:A total of 97 AECOPD patients with invasive MV were screened in the ICUs of the First People's Hospital of Lianyungang.The conventional EN group(stage Ⅰ)and early standardized EN group(stage Ⅱ)included 46 and 51 patients,respectively.ESMcsa loss and GDF-15 levels on days 1 and 7 of ICU admission and 28-day survival rates were analyzed.RESULTS:On day 7,the ESMcsa of the early standardized EN group was significantly higher than that of the conventional EN group,while the plasma GDF-15 levels were significantly lower than those in the conventional EN group(ESMcsa:28.426±6.130 cm^(2) vs.25.205±6.127 cm^(2);GDF-15:1661.608±558.820 pg/mL vs.2541.000±634.845 pg/mL;all P<0.001).The 28-day survival rates of the patients in the early standardized EN group and conventional EN group were 80.40%and 73.90%,respectively(P=0.406).CONCLUSION:ESMcsa loss in AECOPD patients with MV was correlated with GDF-15 levels,both of which indicated acute muscular atrophy and skeletal muscle dysfunction.Early standardized EN may prevent acute muscle loss and intensive care unit-acquired weakness(ICU-AW)in AECOPD patients.
基金Supported by the National Natural Science Foundation of China(Nos.41773009,41873002)the Stake Key Laboratory of Geological Processes and Mineral Resources(No.GPMR201708)+2 种基金the National Science Foundation for Post-doctoral Scientists of China(No.2018M640660)the Taishan Scholar Program of Shandong(No.TS201712075)the AoShan Talents Cultivation Program Supported by Qingdao National Laboratory for Marine Science and Technology(No.2017ASTCP-OS07)。
文摘A number of high-temperature processes(e.g.,melt-rock reactions,metasomatism,partial melting)can produce signifi cant Ca isotopic fractionation and heterogeneity in the mantle,but the mechanism for such fractionation remains obscure.To investigate the eff ect of mantle partial melting on Ca isotopic fractionation,we reported high-precision Ca isotopic compositions of depleted mid-ocean ridge basalts(MORBs)from the East Pacifi c Rise and Ecuador Rift in the northeastern Pacifi c.Theδ44/40 Ca of these MORB samples exhibit a narrow variation from 0.84‰to 0.88‰with an average of 0.85‰±0.03‰,which are similar to those of reported MORBs(0.83‰±0.11‰)and back-arc basin basalts(BABBs,0.80‰±0.08‰)in literature,but are lower than the estimate value for the bulk silicate Earth(BSE,0.94‰±0.05‰).The lowδ44/40 Ca signatures of MORB samples in this study cannot be caused by fractional crystallization,since intermediate-mafi c diff erentiation has been demonstrated having only limited eff ects on Ca isotopic fractionation.Instead,the off set ofδ44/40 Ca between MORBs and the BSE is most likely produced by mantle partial melting.During this process,the light Ca isotopes are preferentially transferred to the melt,while the heavy ones tend to stay in the residue,which is consistent with the fact thatδ44/40 Ca of melt-depleted peridotites increases with partial melting in literature.The behavior of Ca isotopes during mantle partial melting is closely related to the inter-mineral(Cpx and Opx)Ca isotopic fractionation and melting mineral modes.Mantle partial melting is one of the common processes that can induce lowerδ44/40 Ca values in basalts and Ca isotopic heterogeneity in Earth’s mantle.
基金supported by the National Key R&D Program of China (No. 2017YFC0602000)the China Geological Survey Project (Nos. DD20191001 and DD20189410)。
文摘The vector transformation and pole reduction from the total-field anomaly are signifi cant for the interpretation.We examined these industry-standard processing procedures in the Fourier domain.We propose a novel iteration algorithm for regional magnetic anomalies transformations to derive the vertical-component data from the total-field measurements with the variation in the core-fi eld direction over the region.Additionally,we use the same algorithm to convert the calculated vertical-component data into the corresponding data at the pole and realize the processing of diff erential reduction to the pole(DRTP).Unlike Arkani-Hamed’s DRTP method,the two types of iterative algorithms have the same forms,and DRTP is realized by implementing this algorithm twice.The synthetic model’s calculation results show that the method has high accuracy,and the fi eld data processing confi rms its practicality.
文摘In this paper, we present a brief survey on the updated theory of backward stochas-tic Volterra integral equations （BSVIEs, for short）. BSVIEs are a natural generalization of backward stochastic diff erential equations （BSDEs, for short）. Some interesting motivations of studying BSVIEs are recalled. With proper solution concepts, it is possible to establish the corresponding well-posedness for BSVIEs. We also survey various comparison theorems for solutions to BSVIEs.
基金Supported by the Seed Improvement Project of Shandong Province of China(No.2017LZGC009)the Major Scientifi c and Technological Innovation Project of Shandong Provincial Key Research and Development Program(No.2019JZZY020706)。
文摘Haliotis discus hannai is an important marine economic species in China.Its egg color was found to be associated with economic traits,which provides a new idea for breeding.However,the molecular mechanism of the egg-color formation has not been reported.Thus,the pigment composition and comparative transcriptome analyses of H.discus hannai with green and gray egg color were conducted using high-performance liquid chromatography(HPLC)and RNA-Seq methods.Results show that individuals with green and gray eggs both possess the fucoxanthin.Lutein existed in gray-egged individuals,but not in green-egged individuals.In transcriptome analysis,272310 unigenes were received from 461162 transcripts with a mean length of 985 bp and N50 of 1524 bp,respectively.A total of 185 unigenes were identifi ed as diff erentially expressed genes(DEGs).The DEGs involved in“fl avin-containing compound metabolic process”,“melanosome”,“glutathione metabolism”,and“cytochrome b6f complex”were likely related to the formation of the egg color.Our results provide foundational information for the functional analysis of egg-color related genes and are benefi cial to the selective breeding of H.discus hannai.
文摘Larix olgensis A.Henry is a fast-growing tree used for aff orestation in northeastern China and has great ecological and economic value.For studying developmental genes in the xylem of this species,we investigated the Myb transcription factor family,one of the largest families of transcription factors in plants,which plays an important role in the regulation of lignifi cation in plant secondary walls.By sequencing a L.olgensis cDNA library using the Illumina HiSeq2500 high-throughput sequencing platform,we obtained 58,683 unigene sequences,of which 16,554 unigenes were longer than 1000 bp,accounting for 28.2%of the total database.The alignment of these genes with the GO,COG,KEGG,Swiss-Prot and NR databases resulted in annotated 29,350 unigenes.We obtained a total of 1460 differentially expressed genes,of which 453 were upregulated and 1007 were downregulated at the two developmental stages analyzed.The gene annotations showed a wide range of biological functions and metabolic pathways.The 10 Myb transcription factors that were obtained from the diff erentially expressed genes were analyzed by real-time quantitative PCR(qRT-PCR).The results showed that four Myb transcription factors may be associated with xylem development in L.olgensis.Due to the large genome size of conifers,genomics research on these species has lagged behind that for other plant groups.Our data provide the basis for further studies on xylem development in L.olgensis.
