Zingiber striolatum Diels （Zingiberaceae） is an edible plant resource in the Chinese folk with special efficacy in relieving diabetes and constipation, which has been documented in the Compendium ofMateria Medica. H...Zingiber striolatum Diels （Zingiberaceae） is an edible plant resource in the Chinese folk with special efficacy in relieving diabetes and constipation, which has been documented in the Compendium ofMateria Medica. However, its hypoglycemic activity and constituents have not been reported yet. In the present study, we evaluated the hypoglycemic activity of Z. striolatum in insulin-resistant HepG2 cells, and we developed ultra performance liquid chromatography coupled with tandem mass spectrometry （UPLC-MS/MS） based chemical profiling method for rapid analysis of Z. striolatum. As a result, the ethanol extract from Z. striolatum showed significant hypoglycemic activity in HepG2 cells, and 22 flavonoids compounds were tentatively characterized by comparing the retention time and mass spectrometry data. In conclusion, the method of hypoglycemic screening in insulin-resistant HepG2 cells coupled with UPLC-MS/MS is a feasible and credible technique to separate and identify the active constituents in complex matrices of traditional Chinese medicine.展开更多
基金Pearl River S&T Nova Program of Guangzhou(Grant No.201506010061)Foundation for Distinguished Young Teachers in Higher Education of Guangdongand special funds for cultivating Guangdong college students' scientific and technological innovation("Climbing plan",Grant No.pdjh2015b0277)
文摘Zingiber striolatum Diels （Zingiberaceae） is an edible plant resource in the Chinese folk with special efficacy in relieving diabetes and constipation, which has been documented in the Compendium ofMateria Medica. However, its hypoglycemic activity and constituents have not been reported yet. In the present study, we evaluated the hypoglycemic activity of Z. striolatum in insulin-resistant HepG2 cells, and we developed ultra performance liquid chromatography coupled with tandem mass spectrometry （UPLC-MS/MS） based chemical profiling method for rapid analysis of Z. striolatum. As a result, the ethanol extract from Z. striolatum showed significant hypoglycemic activity in HepG2 cells, and 22 flavonoids compounds were tentatively characterized by comparing the retention time and mass spectrometry data. In conclusion, the method of hypoglycemic screening in insulin-resistant HepG2 cells coupled with UPLC-MS/MS is a feasible and credible technique to separate and identify the active constituents in complex matrices of traditional Chinese medicine.
