The objective of the study was to evaluate the anti-diabetic activities of methanol extracts of Aloe camperi (AC), Meriandra dianthera (MD) and a polyherbal drug (PH) in diabetes induced Wistar albino rats. A single d...The objective of the study was to evaluate the anti-diabetic activities of methanol extracts of Aloe camperi (AC), Meriandra dianthera (MD) and a polyherbal drug (PH) in diabetes induced Wistar albino rats. A single dose of alloxan monohydrate (150 mg/kg, i.p.) was used to induce diabetes mellitus (DM). Diabetes was confirmed by the elevated blood glucose levels determined after 72 h of induction. Animals with mean fasting blood glucose (FBG) level more than 200 mg/dl were recruited for the experiment. The herbal extracts at doses of 200 and 400 mg/kg and standard drugmetformin (5 mg/kg) were administered orally to the diabetic rats for 21 days and the FBG level was estimated on 0, 7, 14 and 21 days. The herbal extracts showed dose-dependent fall in FBG levels and the result exhibited very significant (P < 0.001) decreases in FBG level by the end of the experimental day as compared to the diabetic control. The highest antihyperglycemic effect was observed by MD extract at 400 mg/kg and was comparable to the standard drug. Oral glucose tolerance test (OGTT) was also conducted on normal rats and thus glucose at 2 g/kg per body weight was loaded via oral gavage to all groups 30 min after extract administration. All the groups showed significant increase (P P The hyperglycemia with glucose challenge was significantly brought down (P < 0.001) by all herbal extracts at 60 and 120 min relative to the negative control. Moreover, acute oral toxicity tests was conducted based on the protocols of OECD-425 and thus the LD50 of the herbal extracts was estimated to be greater than 2000 mg/kg. Statistical analysis was performed using One-Way ANOVA followed by Dunnett’s test for multiple comparisons, and values of P < 0.05 were considered as statistically significant.展开更多
利用水蒸汽蒸馏法提取宁德产小鱼仙草(M osla d ianthera M ax im)挥发油,得油率为0.50%.应用色谱/质谱/数据系统联用方法分析了其挥发油的化学组成,检测到93个峰,共鉴定了62种化学成分并测定其相对含量,占总含量的98.114%.其中,香荆芥...利用水蒸汽蒸馏法提取宁德产小鱼仙草(M osla d ianthera M ax im)挥发油,得油率为0.50%.应用色谱/质谱/数据系统联用方法分析了其挥发油的化学组成,检测到93个峰,共鉴定了62种化学成分并测定其相对含量,占总含量的98.114%.其中,香荆芥酚(27.229%)、香芹酮(12.326%)、百里香酚(11.027%)、β-石竹烯(7.246%)、柠檬烯(6.209%)、2-甲基-5-(1-异丙基)-乙酰苯酚(5.932%)、间伞花烃(4.982%)、艹律草烯(4.496%)为主要成分.对7种供试细菌的体外抑菌实验表明,该挥发油对金黄色葡萄球菌(S taphy lococcus aureus)、大肠杆菌(E scherich ia coli)、藤黄八叠球菌(S arcina lutea)、枯草芽孢杆菌(B acillussubtilis)、普通变形杆菌(P roteas vu lg aris)、短小芽孢杆菌(B acillus pum ilus)、甘薯薯瘟病原细菌(F asariumaxy ssp oram)有一定的抑制作用.展开更多
文摘The objective of the study was to evaluate the anti-diabetic activities of methanol extracts of Aloe camperi (AC), Meriandra dianthera (MD) and a polyherbal drug (PH) in diabetes induced Wistar albino rats. A single dose of alloxan monohydrate (150 mg/kg, i.p.) was used to induce diabetes mellitus (DM). Diabetes was confirmed by the elevated blood glucose levels determined after 72 h of induction. Animals with mean fasting blood glucose (FBG) level more than 200 mg/dl were recruited for the experiment. The herbal extracts at doses of 200 and 400 mg/kg and standard drugmetformin (5 mg/kg) were administered orally to the diabetic rats for 21 days and the FBG level was estimated on 0, 7, 14 and 21 days. The herbal extracts showed dose-dependent fall in FBG levels and the result exhibited very significant (P < 0.001) decreases in FBG level by the end of the experimental day as compared to the diabetic control. The highest antihyperglycemic effect was observed by MD extract at 400 mg/kg and was comparable to the standard drug. Oral glucose tolerance test (OGTT) was also conducted on normal rats and thus glucose at 2 g/kg per body weight was loaded via oral gavage to all groups 30 min after extract administration. All the groups showed significant increase (P P The hyperglycemia with glucose challenge was significantly brought down (P < 0.001) by all herbal extracts at 60 and 120 min relative to the negative control. Moreover, acute oral toxicity tests was conducted based on the protocols of OECD-425 and thus the LD50 of the herbal extracts was estimated to be greater than 2000 mg/kg. Statistical analysis was performed using One-Way ANOVA followed by Dunnett’s test for multiple comparisons, and values of P < 0.05 were considered as statistically significant.
