Differentiating pasteurized milk and reconsti-tuted milk by scientific approach was necessary to defend consumer from economic fraud of wrong labeling. In this paper 2DGE (2 Dimen-sional Gel Electrophoresis)-coomassie...Differentiating pasteurized milk and reconsti-tuted milk by scientific approach was necessary to defend consumer from economic fraud of wrong labeling. In this paper 2DGE (2 Dimen-sional Gel Electrophoresis)-coomassie brilliant blue staining method was employed and sig-nificant color intensity changing was observed among raw milk, pasteurized milk, UHT milk and reconstituted milk. For example, the intensity of 10 protein spots including casein and lac-toglobulin reduced more than two folds from pasteurized milk to reconstituted milk. However, DIGE (Differential Gel Electrophoresis) assay showed that the majority protein remained simi-lar level from pasteurized milk to reconstituted milk. Therefore the color fading of coomassie brilliant blue stained 2D gels may be due to other biochemical reaction, such as Maillard reaction, instead of protein degradation. Stability of 2DGE pattern was confirmed by running six gels of the same sample in parallel and software analysis showed that all proteins were at similar level. Two commercialized pasteurized milk samples and one reconstituted milk sample were tested by 2DGE-coomassie blue staining method and re-constituted milk could be easily identified.展开更多
Fruit of Lonicera caerulea L. is a rich source of phenolic compounds such as phenolic acids as well as anthocyanins,proanthocyanidins and other flavonoids,which display potential health-promoting effects. However,its ...Fruit of Lonicera caerulea L. is a rich source of phenolic compounds such as phenolic acids as well as anthocyanins,proanthocyanidins and other flavonoids,which display potential health-promoting effects. However,its genetics and genomic information is limited. Transcriptome and digital gene expression( DGE) profiles of fruit at four different development stages were compared. Related genes with anthocyanin biosynthesis were compared using real-time quantitative PCR( qRT-PCR). RNA-Seq generated 6. 26-Gb clean reads,which was then de novo assembled into 45,656 UniG enes with a mean length 837 bp. A total of 7 928 unigenes were annotated into 32 known metabolic or signaling pathways in which a few primary,intermediate,and secondary metabolic pathways are directly related to fruit quality. The result of DGE and qRT-PCR indicates that UFGT( comp20132_c0) may play a key role in honeysuckle coloration. This provides a reference for the study of complicated metabolism in non-model perennial species.展开更多
文摘Differentiating pasteurized milk and reconsti-tuted milk by scientific approach was necessary to defend consumer from economic fraud of wrong labeling. In this paper 2DGE (2 Dimen-sional Gel Electrophoresis)-coomassie brilliant blue staining method was employed and sig-nificant color intensity changing was observed among raw milk, pasteurized milk, UHT milk and reconstituted milk. For example, the intensity of 10 protein spots including casein and lac-toglobulin reduced more than two folds from pasteurized milk to reconstituted milk. However, DIGE (Differential Gel Electrophoresis) assay showed that the majority protein remained simi-lar level from pasteurized milk to reconstituted milk. Therefore the color fading of coomassie brilliant blue stained 2D gels may be due to other biochemical reaction, such as Maillard reaction, instead of protein degradation. Stability of 2DGE pattern was confirmed by running six gels of the same sample in parallel and software analysis showed that all proteins were at similar level. Two commercialized pasteurized milk samples and one reconstituted milk sample were tested by 2DGE-coomassie blue staining method and re-constituted milk could be easily identified.
基金Supported by Agricultural Science and Technology Innovation Project of China
文摘Fruit of Lonicera caerulea L. is a rich source of phenolic compounds such as phenolic acids as well as anthocyanins,proanthocyanidins and other flavonoids,which display potential health-promoting effects. However,its genetics and genomic information is limited. Transcriptome and digital gene expression( DGE) profiles of fruit at four different development stages were compared. Related genes with anthocyanin biosynthesis were compared using real-time quantitative PCR( qRT-PCR). RNA-Seq generated 6. 26-Gb clean reads,which was then de novo assembled into 45,656 UniG enes with a mean length 837 bp. A total of 7 928 unigenes were annotated into 32 known metabolic or signaling pathways in which a few primary,intermediate,and secondary metabolic pathways are directly related to fruit quality. The result of DGE and qRT-PCR indicates that UFGT( comp20132_c0) may play a key role in honeysuckle coloration. This provides a reference for the study of complicated metabolism in non-model perennial species.
