Variation in the limit dextrinase activity of barley malt, and the relationships between limit dextrinase activity and malt quality parameters were investigated using eight cultivars grown at seven diverse locations i...Variation in the limit dextrinase activity of barley malt, and the relationships between limit dextrinase activity and malt quality parameters were investigated using eight cultivars grown at seven diverse locations in China for two successive years. Limit dextrinase activity varied with genotype and location, with the levels ranging from 0.245 U/g to 0.980 U/g. The results showed that the variation in limit dextrinase activity was more attributable to the environment (location and year) than to the genotype. The response of limit dextrinase activity to the environment differed markedly among cultivars, and was reflected by large difference in coefficient of variation of cultivars across diverse locations. Regression analysis showed that limit dextrinase activity was negatively correlated with malt viscosity (r=?0.52, P<0.01), positively correlated with Kolbach index (r=0.38, P<0.01) and malt extract (r=0.30, P<0.05), but had no significant correlation with malt protein content and diastatic power.展开更多
High malting quality of barley （Hordeum vulgare L.） relies on many traits, such as β-amylase and limit dextrinase activities and β-glucan and protein fraction contents. In this study, interval mapping was utilized...High malting quality of barley （Hordeum vulgare L.） relies on many traits, such as β-amylase and limit dextrinase activities and β-glucan and protein fraction contents. In this study, interval mapping was utilized to detect quantitative trait loci （QTLs） affecting these malting quality parameters using a doubled haploid （DH） population from a cross of CM72 （six-rowed） by Gairdner （two-rowed） barley cultivars. A total of nine QTLs for eight traits were mapped to chromosomes 3H, 4H, 5H, and 7H. Five of the nine QTLs mapped to chromosome 3H, indicating a possible role ofloci on chromosome 3H on malting quality. The phenotypic variation accounted by individual QTL ranged from 8.08% to 30.25%. The loci of QTLs for D-glucan and limit dex- trinase were identified on chromosomes 4H and 5H, respectively. QTL for hordeins was coincident with the region of silica eluate （SE） protein on 3HS, while QTLs for albumins, globulins, and total protein exhibited overlapping. One locus on chromosome 3H was found to be related to （J-amylase, and two loci on chromosomes 5H and 7H were found to be associated with glutelins. The identification of these novel QTLs controlling malting quality may be useful for marker-assisted selection in improving barley malting quality.展开更多
Based on five different species of barley, the foot layer analytic method was used to examine the activity and heat-resistance of the limiting dextrinase. The study was conducted on the dynamic changes of several type...Based on five different species of barley, the foot layer analytic method was used to examine the activity and heat-resistance of the limiting dextrinase. The study was conducted on the dynamic changes of several types of the dextrinase in barley germinating process, the effect of temperature on the dextrinase and the divergence of dextrinase in different barley variety. The probability of the dextrinase that as reference index is展开更多
Global change is bringing barley with more frequency of suffering from high temperature.However,little has been known about the influence of high temperature on malt quality traits.In this study,we investigated the im...Global change is bringing barley with more frequency of suffering from high temperature.However,little has been known about the influence of high temperature on malt quality traits.In this study,we investigated the impact of 1-wk heat stress(32°C/26°C,day/night,12 h/12 h)initiating from the 7th(HT7)and 14th(HT14)days after heading on some grain and malt quality traits of two barley cultivars.In comparison with normal temperature(24°C/18°C,day/night,12 h/12 h),heat stress significantly reduced kernel weight,seed setting rate and grains per spike:HT7 having a larger effect than HT14.Meanwhile,total protein andβ-glucan contents,andβ-amylase and limit dextrinase activities were significantly increased under high temperature,with HT7-treated plants showing larger changes.Moreover,the different changes of four protein fractions under heat stress were found in the two barley cultivars,indicating the possibility of breaking positive association between protein content and enzyme activity.展开更多
基金Project supported by the National Natural Science Foundation of China (Nos. 30270779 and 30471022) and the Specialized Research Fund for the Doctoral Program of Higher Education (No. 20020335028), China
文摘Variation in the limit dextrinase activity of barley malt, and the relationships between limit dextrinase activity and malt quality parameters were investigated using eight cultivars grown at seven diverse locations in China for two successive years. Limit dextrinase activity varied with genotype and location, with the levels ranging from 0.245 U/g to 0.980 U/g. The results showed that the variation in limit dextrinase activity was more attributable to the environment (location and year) than to the genotype. The response of limit dextrinase activity to the environment differed markedly among cultivars, and was reflected by large difference in coefficient of variation of cultivars across diverse locations. Regression analysis showed that limit dextrinase activity was negatively correlated with malt viscosity (r=?0.52, P<0.01), positively correlated with Kolbach index (r=0.38, P<0.01) and malt extract (r=0.30, P<0.05), but had no significant correlation with malt protein content and diastatic power.
基金Project supported by the National Natural Science Foundation of China (Nos. 30630047 and 30771281)the Hi-Tech Research and Development Program (863) of China (No. 2006AA10Z1C3)the Ministry of Education and the State Administration of Foreign Experts Affairs (111 Project) of China (No. B06014)
文摘High malting quality of barley （Hordeum vulgare L.） relies on many traits, such as β-amylase and limit dextrinase activities and β-glucan and protein fraction contents. In this study, interval mapping was utilized to detect quantitative trait loci （QTLs） affecting these malting quality parameters using a doubled haploid （DH） population from a cross of CM72 （six-rowed） by Gairdner （two-rowed） barley cultivars. A total of nine QTLs for eight traits were mapped to chromosomes 3H, 4H, 5H, and 7H. Five of the nine QTLs mapped to chromosome 3H, indicating a possible role ofloci on chromosome 3H on malting quality. The phenotypic variation accounted by individual QTL ranged from 8.08% to 30.25%. The loci of QTLs for D-glucan and limit dex- trinase were identified on chromosomes 4H and 5H, respectively. QTL for hordeins was coincident with the region of silica eluate （SE） protein on 3HS, while QTLs for albumins, globulins, and total protein exhibited overlapping. One locus on chromosome 3H was found to be related to （J-amylase, and two loci on chromosomes 5H and 7H were found to be associated with glutelins. The identification of these novel QTLs controlling malting quality may be useful for marker-assisted selection in improving barley malting quality.
文摘Based on five different species of barley, the foot layer analytic method was used to examine the activity and heat-resistance of the limiting dextrinase. The study was conducted on the dynamic changes of several types of the dextrinase in barley germinating process, the effect of temperature on the dextrinase and the divergence of dextrinase in different barley variety. The probability of the dextrinase that as reference index is
基金the National Natural Science Foundation of China(31620103912)the earmarked fund for China Agriculture Research System(CARS-05)the Jiangsu Collaborative Innovation Center for Modern Crop Production,China(JCIC-MCP)for their financial supports to this research,including experimental implementation,sampling and data analysis.
文摘Global change is bringing barley with more frequency of suffering from high temperature.However,little has been known about the influence of high temperature on malt quality traits.In this study,we investigated the impact of 1-wk heat stress(32°C/26°C,day/night,12 h/12 h)initiating from the 7th(HT7)and 14th(HT14)days after heading on some grain and malt quality traits of two barley cultivars.In comparison with normal temperature(24°C/18°C,day/night,12 h/12 h),heat stress significantly reduced kernel weight,seed setting rate and grains per spike:HT7 having a larger effect than HT14.Meanwhile,total protein andβ-glucan contents,andβ-amylase and limit dextrinase activities were significantly increased under high temperature,with HT7-treated plants showing larger changes.Moreover,the different changes of four protein fractions under heat stress were found in the two barley cultivars,indicating the possibility of breaking positive association between protein content and enzyme activity.
