A morphology-based growth stage system should describe the growth and development of a crop and thereby help farmers and agronomists in formulating reasonable managementmeasures conducive to the development of marketa...A morphology-based growth stage system should describe the growth and development of a crop and thereby help farmers and agronomists in formulating reasonable managementmeasures conducive to the development of marketable products.However,existing growth stage systems for soybean are either based on plant growth or covered particular phases of flower or pod development,making it difficult to use for tracking the entire growth period of individual flowers and pods.Therefore,the first flower and pod,located at the base of the primary raceme in the eighth trifoliate node of the main stem,were chosen to illustrate growth dynamics during the full reproductive period.The size and fresh weight of the primary raceme in the eighth trifoliate leaf axil,the first flower and pod,the pistils in the first flower,and seeds in the first pod were examined,and the growth of these organs was depicted.Integrating the morphological characteristics and growth features of flowers and pods,as well as existing growth stage systems,the growth and development were delineated in 13 stages.In detail,we classified the flower phase based on the relative positions of floral components,inspired by the ratio of bract to flower used for staging,refined the lag phase proposed previously,retained the use of pod length to define the early pod phase,and innovatively described the late pod phase by the seed appearance.The developmental events in each stage of flower and pod were distinctive and closely connected to the corresponding morphology.Taken together,a more detailed growth stage system for describing individual flowers and pods in soybean was established.This system will serve as a valuable research tool for describing the development,gene expression,and cellular metabolism associated with the formation of flowers,pods,and seeds.展开更多
Economic growth and fair distribution are two major goals pursued in economic development.Through a comparative analysis of economies such as those in Europe and the United States,it is found that:Economic growth does...Economic growth and fair distribution are two major goals pursued in economic development.Through a comparative analysis of economies such as those in Europe and the United States,it is found that:Economic growth does not have to be conditional on the expansion of income gap;growth will not automatically lead to fair distribution;efficiency and fairness can occur simultaneously;the transformation of the economic development mode can be achieved through income distribution.A shared development model that takes into account both growth and fairness is the choice to achieve sustained and stable economic growth.It is necessary to deepen the reform of the income distribution system,improve social security and other measures to promote the development achievements to benefit the majority of workers more,and achieve both efficiency and fairness at the same time.展开更多
Nowadays,with the continuous deepening of quality-oriented education in China and the increasing demands of parents,especially the implementation of the new curriculum design concept,tremendous changes have taken plac...Nowadays,with the continuous deepening of quality-oriented education in China and the increasing demands of parents,especially the implementation of the new curriculum design concept,tremendous changes have taken place in teaching methods,teaching contents,teaching means,and learning approaches,which pose new challenges to the professional growth of preschool teachers.At present,preschool teachers are not only required to have a sense of responsibility and love,but also to have rich professional knowledge and skills,as well as new creative thinking and research ability.Even if the preschool teachers who have performed very well in traditional education are now struggling to adapt to the actual needs of educational development,which requires preschool teachers to continuously develop in the direction of specialization.This article will focus on how to promote the professional growth and development of preschool teachers to analyze and elaborate.展开更多
BACKGROUND Newborns with low-birth-weight may lag behind those with normal-birth-weight in terms of growth,development,and nutritional status,which increases mothers’concerns about the child’s future health and lead...BACKGROUND Newborns with low-birth-weight may lag behind those with normal-birth-weight in terms of growth,development,and nutritional status,which increases mothers’concerns about the child’s future health and leads to anxiety and depression.Providing nutritional support to newborns with low-birth-weight to facilitate optimal growth and development may help alleviate maternal anxiety and depre-ssion.RESULTS No significant difference in baseline data was observed between the two groups(P>0.05).Milk intake and TBIL levels in Group S were significantly better than those in Group L at 1,2,3,and 4 weeks after nutritional support(P<0.05).No significant differences in vomiting,abdominal distension,constipation,diarrhea,and residual adverse reactions were observed between the two groups during nutritional support(P>0.05).Group S had a significantly reduce of fewer complications(i.e.,neonatal necrotizing enterocolitis and infection)than did Group L during nutritional support(P<0.05).Before nutritional support,no significant differences in developmental indicators(weight,length,and head circumference)were noted between the two groups(P>0.05).However,after nutritional support,the weight,length,and head circumference of both groups were higher than those before intervention,with Group S having significantly higher values than did Group L(P<0.05).Mothers of children who received nutritional support had decreased SAS and SDS scores,with Group S mothers having significantly lower scores than did Group L mothers(P<0.05).CONCLUSION Enteral nutrition support had significant effects on newborns weighing<2 kg within 24 hours of birth.In particular,it promoted the maturation of gastrointestinal function,enhanced nutrient absorption,promoted optimal short-term growth and development,and alleviated the mother’s anxiety and depression.展开更多
BACKGROUND Unintended pregnancy occurs when an individual or couple conceives without planning or desire,which can potentially affect a child’s physical,mental,and social well-being.This can then lead to long-term so...BACKGROUND Unintended pregnancy occurs when an individual or couple conceives without planning or desire,which can potentially affect a child’s physical,mental,and social well-being.This can then lead to long-term socioeconomic challenges for families and communities.Although its impact on child growth and development is a pressing concern,research remains limited particularly in multicenter settings.AIM To examine the long-term consequences of unintended pregnancy on the critical years of early childhood growth and development.METHODS This analytical observational study employed a case-control design and was conducted in research centers across Indonesia,encompassing those located in Central Java,Lampung,Bali,and West Nusa Tenggara.A total of 700 children aged≤5 years with histories of intended or unintended pregnancies participated.Data collection involved structured interviews and direct anthropometric and developmental assessments.Data analyses were conducted using multivariate statistics and partial least squares structural equation modeling.RESULTS Unintended pregnancy was found to have a statistically significant effect on both child growth(t=8.178;P<0.001)and child development(t=25.688;P<0.001).Key growth problems identified included underweight,undernutrition,abnormal head circumference,and stunting.Developmental challenges prominently associated with unintended pregnancy included behavioral and emotional disorders,autism spectrum disorder,attentiondeficit/hyperactivity disorder,social and motor skill deficits,as well as visual and hearing impairments.CONCLUSION Unintended pregnancy significantly affects child growth and development,underscoring the need for early intervention,quality prenatal care,and strengthened family planning policies.展开更多
BACKGROUND First-time mothers may encounter various problems during postpartum,which can result in negative emotions that can affect infant care.In today’s Internet era,continuous nursing services can be provided to ...BACKGROUND First-time mothers may encounter various problems during postpartum,which can result in negative emotions that can affect infant care.In today’s Internet era,continuous nursing services can be provided to mothers and their babies after delivery through Internet-based platforms.This approach can help reduce negative emotions of primiparas and promote better health for both mothers and babies.AIM To explore the effect of Internet Plus-based postpartum healthcare services on postpartum depression of primiparas and neonatal growth and development and thus provide a scientific basis for strengthening postpartum healthcare measures and better protect maternal and child health.METHODS The study retrospectively collected data of primiparas and their newborns who underwent prenatal examination and successfully delivered at the Ninth People’s Hospital of Suzhou City.The observation group included 30 primiparas and their newborns who received Internet Plus-based postpartum healthcare services between July and December 2024.According to the principle of matching(1:1)control study,the control group included 30 primiparas and their newborns who received routine postpartum healthcare services between January and June 2024.The maternal role adaptation questionnaire scores,breastfeeding rates,Edinburgh postnatal depression scale(EPDS)scores,and newborn growth and development(height,head circumference,and weight)were compared between the two groups at the time of discharge after delivery and 6-week postpartum follow-up.RESULTS Upon hospital discharge,the two groups did not demonstrate significant differences in maternal role adaptation scores,breastfeeding rates,EPDS scores,as well as newborn height,head circumference,and weight at birth(P>0.05).At the 6-week postpartum follow-up,the maternal role adaptation score and breastfeeding rate were higher in the observation group than in the control group(P<0.05).In addition,one case of postpartum depression was reported in the observation group and eight in the control group.Moreover,the control group exhibited a significant increase in EPDS scores compared with scores at hospital discharge(P<0.05),whereas the observation group showed only a marginal,nonsignificant increase in EPDS scores(P>0.05).The EPDS score of the observation group was significantly lower than that of the control group(P<0.05),indicating a lower risk of postpartum depression in the observation group.The length,head circumference,and weight of the newborns 6 weeks after birth were increased compared with those at birth,and the growth rate was higher in the observation group than in the control group(P<0.05),indicating better growth and development in the observation group.CONCLUSION Internet Plus-based postpartum healthcare services improve maternal role adaptation,increase breastfeeding rates,mitigate postpartum depression risk,and promote neonatal growth and development in primiparas.展开更多
Related to ABI3 and VP1(RAV)transcription factors belong to the AP2 and B3 superfamily.RAVs genes have been reported to be involved in plant growth and development regulation.This study screened three RAV genes from M...Related to ABI3 and VP1(RAV)transcription factors belong to the AP2 and B3 superfamily.RAVs genes have been reported to be involved in plant growth and development regulation.This study screened three RAV genes from Medicago truncatula and named one of them MtRAV1.The MtRAV1 overexpressing plants exhibits traits such as plant dwarfing,delayed flowering,reduced leaf and floral organs,increased branching,and reduced pods and seeds.Gene expression analysis results showed that overexpression of Mt RAV1 inhibited the expression of Flowering Locus T(MtFTa1),Suppressor of Overexpression of CO1(MtSOC1),GA3-oxidase1(MtGA3OX1),DWARF14(MtD14)and Carotenoid Cleavage Dioxygenase7(MtCCD7).To further investigate the regulation pathway involved by MtRAV1,RNA-sequencing(RNA-seq)and DNA affinity purification sequencing(DAP-seq)analysis were conducted.RNA-seq results indicated that MtRAV1 might affect plant growth and development by regulating some genes in photosynthesis,circadian rhythm and plant hormone signaling pathways,especially the auxin signaling pathway.Conjoint analysis of DAP-seq and RNA-seq revealed that Mt RAV1 might inhibit the expression of Ferredoxin(Mt Fd-l3),Light-harvesting Chlorophyll a/b Binding Protein 1(Mt Lhcb-l2)and Small Auxin Up-regulated RNA(Mt SAUR-l),which related to photosystem II and auxin signaling pathway.Summarily,MtRAV1 was preliminarily proven to be a key growth inhibitory factor in M.truncatula.展开更多
[Objectives]To provide a reference for the promotion of appropriate row spacing configuration modes for cotton planting in the Bortala Mongol Autonomous Prefecture of Xinjiang.[Methods]Xinluzao 63 was employed as the ...[Objectives]To provide a reference for the promotion of appropriate row spacing configuration modes for cotton planting in the Bortala Mongol Autonomous Prefecture of Xinjiang.[Methods]Xinluzao 63 was employed as the research subject to examine the effects of three different configuration modes:three rows with one film,four rows with one film,and six rows with one film,on the growth and development of cotton,as well as on yield and the amount of residual film in the field.[Results]In comparison to the configuration modes of four rows with one film and six rows with one film,the development process in the row spacing configuration mode of three rows with one film was accelerated by 1-4 d.This configuration mode exhibited variability in several agronomic traits,particularly in plant height,the number of fruiting branches per plant,and the number of leaves per plant,with the observed trend indicating T3>T2>T1.Conversely,the height of the first fruiting branch node displayed an inverse trend.In terms of yield composition,no significant differences were observed in boll weight and yield among various configuration modes.However,T3 exhibited the highest boll weight at 5.68 g and a yield of 462.67 kg/667 m 2.Additionally,significant differences were noted in harvesting density and the number of bolls per plant.T3 demonstrated the lowest harvesting density at 1.11×104 plants/666.7 m 2,the highest number of bolls per plant at 8.63,and the highest boll opening rate at 97.48%.Furthermore,T3 also resulted in the least amount of agricultural film residue during the current season.[Conclusions]Among the three planting configuration modes examined,the low-density planting configuration mode consisting of three rows and one film demonstrated a significant advantage at the individual plant level.This approach yielded results comparable to those of the high density planting configuration mode while also reducing costs.Furthermore,low density planting positively influenced the cotton boll opening rate,leading to a decreased amount of residual film and promoting ecological health within the agricultural land.展开更多
Objective:To explore the positive role of developmental care measures in promoting the growth and development of premature infants,and analyze their specific effects on improving the sleep duration and quality of prem...Objective:To explore the positive role of developmental care measures in promoting the growth and development of premature infants,and analyze their specific effects on improving the sleep duration and quality of premature infants.Methods:A total of 80 premature infants who met the inclusion criteria were selected by convenient sampling method and divided into an experimental group and a control group,with 40 cases in each group.The experimental group received comprehensive nursing intervention of developmental care during the nursing process;the control group adopted the traditional conventional nursing model.Results:There was no statistically significant difference in various physical indicators between the two groups of premature infants before nursing intervention.After nursing intervention,the scores of intellectual development level,psychomotor development index and various physical indicators of premature infants in the experimental group were significantly higher than those in the control group,and the difference was statistically significant.Conclusion:The implementation of developmental care for premature infants can significantly improve their various developmental indicators,which can not only effectively promote the rapid development of premature infants’mental and physical growth,but also improve their sleep quality.展开更多
Repeated wet swelling and dry shrinkage of soil leads to the gradual occurrence of cracks and the formation of a complex fracture network.In order to study the development characteristics and quantitative analysis of ...Repeated wet swelling and dry shrinkage of soil leads to the gradual occurrence of cracks and the formation of a complex fracture network.In order to study the development characteristics and quantitative analysis of cracks in root-soil complex in different growth periods under dry-wet cycles,the alfalfa root-loess complex was in-vestigated during different growth periods under different dry-wet cycles,and a dry-wet cycle experiment was conducted.The crack rate,relative area,average width,total length,and the cracks fractal dimension in the root-soil complex were extracted;the crack development characteristics of plain soil were analyzed under the PG-DwC(dry-wet cycle caused by plant water management during plant growth period),as well as the crack development characteristics of root-soil complex under PG-DWC and EC-DWC(the dry-wet cycles caused by extreme natural conditions such as continuous rain);the effects of plant roots and dry-wet cycles on soil cracks were discussed.The results showed that the average crack width,crack rate,relative crack area,and total crack length of the alfalfa root-loess complex were higher than those of the plain soil during PG-DWC.The result indicated that compared with plain soil during PG-DWC,the presence of plant roots in alfalfa root-soil complex in the same growth period promoted the cracks development to some extent.The alfalfa root-soil complex crack parameters during different growth periods were relatively stable during PG-DWC(O dry-wet cycle).During EC-DWC(1,3,and 5 dry-wet cycles),the alfalfa root-loess complex crack parameters increased with the number of dry-wet cycles during different growth periods.Unlike PG-DWC,the EC-DWC accelerated crack development,and the degree of crack development increased with the number of dry-wet cycles.The existence of plant roots promoted crack development and expansion in the root-soil complex to a certain extent,and the dry-wet cycle certainly promoted crack development and expansion in the root-soil complex.This result contradicts the im-provement in the root-soil complex's macro-mechanical properties during plant growth,due to differences in the mechanical properties of roots and soil.The research results will provide reference for the root soil complex crack development law and the design of slope protection by vegetation.展开更多
Agrochemicals,especially plant growth regulators(PGRs),are extensively used to modulate endogenous phytohormone signals in small quantities,significantly infiuencing plant growth and development.Plant hormones typical...Agrochemicals,especially plant growth regulators(PGRs),are extensively used to modulate endogenous phytohormone signals in small quantities,significantly infiuencing plant growth and development.Plant hormones typically exhibit diverse chemical structures,with common examples including indole rings,terpenoid frameworks,adenine motifs,cyclic lactones,cyclopentanones,and steroidal compounds,which are extensively employed in pesticides.This article explores the interactions and biological activities of small molecules on proteins,enzymes,and other reactive sites involved in the biosynthesis,metabolism,transport,and signal transduction pathways of various plant hormones.Additionally,it analyzes the structure-activity relationships(SARs)of pesticides incorporating these structural motifs to elucidate the relationship between active fragments,pharmacophores,and targets,highlighting the characteristics of potent small molecules and their derivatives.This comprehensive review aims to provide novel perspectives for the development and design of pesticides,offering valuable insights for researchers in the field.展开更多
In the Turkish Economy,there were radical changes in the structure of the economy with the policies of opening up to the outside world and transition to a free market economy in the 1980s,and the last step of this ope...In the Turkish Economy,there were radical changes in the structure of the economy with the policies of opening up to the outside world and transition to a free market economy in the 1980s,and the last step of this opening up and liberalization process was realized with the decision number 32 in 1989.We can say that with the liberalization of capital movements in the 1990s,economic growth and development were tried to be achieved through hot money inflows rather than direct foreign investments.This trend made the economy more open to crises,and for the first time,a crisis occurred in the form of the 1994 economic crisis,which was understood to be caused by hot money.The same economic structure experienced a financial and economic crisis caused by hot money again in November 2000 and February 2001.While the crisis was overcome with the stand-by agreement made with the IMF(International Monetary Fund)and the announced Transition to a Strong Economy program,the economy started to grow rapidly with the ease of using foreign resources,and political stability seems to have enabled this economic growth process to continue uninterruptedly except 2009.There was also a decrease in inflation rates.The same economic structure continued in the period between 2010 and 2020,and the financing need of economic growth was met by outsourcing.However,this process was different from the previous decade and there was no economic and financial crisis other than the sudden increase in exchange rates in 2018.We can say that the sudden exchange rate increase in 2018 was perceived as a harbinger of possible exchange rate shocks in the following years.展开更多
Strip-till(ST),including straw mulching in the inter-row and localized fertilization in the intra-row,is a conservation tillage system for improving soil quality and crop growth.However,the yield advantage of maize un...Strip-till(ST),including straw mulching in the inter-row and localized fertilization in the intra-row,is a conservation tillage system for improving soil quality and crop growth.However,the yield advantage of maize under ST compared to conventional tillage(CT)remains unstable,and the strategies to increase maize yield under ST are unclear.This study aims to understand the physiological mechanism underlining maize yield formation under ST by comparing two maize cultivars,DKM753 and DK517,with contrasting yield performance in ST versus CT systems.Compared to CT,ST resulted in a 4.5%yield increase for DKM753 but a 5.6%decrease for DK517.These yield differences were primarily attributed to variations in grain number per ear(GN).During the rapid growth stage(V14-R3),i.e.,two weeks before and after silking,DKM753 showed a 6.7%increase in maximum growth rate(V_(max))and a 6.3%increase in average growth rate(V)under ST,whereas DK517 exhibited decline of 8.5%in V_(max) and 12.3%in V.Significant positive correlations are observed between V_(max) and V with GN under ST(R^(2)=0.79 and R^(2)=0.90,respectively).Enhanced dry matter accumulation in DKM753 under ST was attributed to increased leaf expansion rates,contributing to a larger photosynthate source.The straw mulching and localized nitrogen fertilization increased root-zone nitrogen availability at silking in ST compared to CT.DKM753 had a greater root system which made better use of the soil N and lead to an increased leaf nitrogen accumulation by 14.9%under ST.It is concluded that maize yield under the strip-till system is determined by grain number per ear,which can be increased by increasing nitrogen accumulation,plant growth,and ear development around silking stage.A sound root system can efficiently utilize soil nitrogen resources under the strip-till system,increasing plant nitrogen accumulation and thereby promoting plant growth.展开更多
We developed a strategy involving an electroactive biofiltration dynamic membrane(EBDM)for wastewater treatment and membrane fouling mitigation.This approach utilizes a cathode potential within an anaerobic dynamic me...We developed a strategy involving an electroactive biofiltration dynamic membrane(EBDM)for wastewater treatment and membrane fouling mitigation.This approach utilizes a cathode potential within an anaerobic dynamic membrane bioreactor to establish a growth equilibrium electroactive fouling layer.Over a 240 day operation period,the EBDM exhibited outstanding performance,characterized by an ultralow fouling rate(transmembrane pressure<2.5 kPa),superior effluent quality(chemical oxygen demand(COD)removal>93%and turbidity 2 nephelometric turbidity units(NTU)),and a 7.2%increase in methane(CH4)productivity.Morphological analysis revealed that the EBDM acted as a biofilter consisting of a structured,interconnected,multilevel dynamic membrane system with orderly clogging.In the EBDM system,the balanced-growth fouling layers presented fewer biofoulants and looser secondary protein structures.Furthermore,the applied electric field modified the physicochemical properties of the biomass,leading to a decrease in fouling potential.Quartz crystal microbalance with dissipation monitoring analysis indicated that growth equilibrium promoted a looser fouling layer with a lower adsorption mass than did the denser,viscoelastic fouling layer observed in the control reactor.Metagenomic sequencing further demonstrated that continuous electrical stimulation encouraged the development of an electroactive fouling layer with enhanced microbial metabolic functionality on the EBDM.This approach selectively modifies metabolic pathways and increases the degradation of foulants.The EBDM strategy successfully established an ordered-clogging,step-filtered,and balanced-growth electroactive fouling layer,achieving a synergistic effect in reducing membrane fouling,enhancing effluent quality,and improving CH_(4)productivity.展开更多
Human brain development is a complex process,and animal models often have significant limitations.To address this,researchers have developed pluripotent stem cell-derived three-dimensional structures,known as brain-li...Human brain development is a complex process,and animal models often have significant limitations.To address this,researchers have developed pluripotent stem cell-derived three-dimensional structures,known as brain-like organoids,to more accurately model early human brain development and disease.To enable more consistent and intuitive reproduction of early brain development,in this study,we incorporated forebrain organoid culture technology into the traditional unguided method of brain organoid culture.This involved embedding organoids in matrigel for only 7 days during the rapid expansion phase of the neural epithelium and then removing them from the matrigel for further cultivation,resulting in a new type of human brain organoid system.This cerebral organoid system replicated the temporospatial characteristics of early human brain development,including neuroepithelium derivation,neural progenitor cell production and maintenance,neuron differentiation and migration,and cortical layer patterning and formation,providing more consistent and reproducible organoids for developmental modeling and toxicology testing.As a proof of concept,we applied the heavy metal cadmium to this newly improved organoid system to test whether it could be used to evaluate the neurotoxicity of environmental toxins.Brain organoids exposed to cadmium for 7 or 14 days manifested severe damage and abnormalities in their neurodevelopmental patterns,including bursts of cortical cell death and premature differentiation.Cadmium exposure caused progressive depletion of neural progenitor cells and loss of organoid integrity,accompanied by compensatory cell proliferation at ectopic locations.The convenience,flexibility,and controllability of this newly developed organoid platform make it a powerful and affordable alternative to animal models for use in neurodevelopmental,neurological,and neurotoxicological studies.展开更多
Developing deep fragmented soft coalbed methane(CBM)can significantly enhance domestic natural gas supplies,reduce reliance on imported energy,and bolster national energy security.This manuscript provides a comprehens...Developing deep fragmented soft coalbed methane(CBM)can significantly enhance domestic natural gas supplies,reduce reliance on imported energy,and bolster national energy security.This manuscript provides a comprehensive review of commonly employed coalbed methane extraction technologies.It then delves into several critical issues in the current stage of CBM exploration and development in China,including the compatibility of existing technologies with CBM reservoirs,the characteristics and occurrence states of CBM reservoirs,critical desorption pressure,and gas generation mechanisms.Our research indicates that current CBM exploration and development technologies in China have reached an internationally advanced level,yet the industry is facing unprecedented challenges.Despite progress in low-permeability,high-value coal seams,significant breakthroughs have not been achieved in exploring other types of coal seams.For different coal reservoirs,integrated extraction technologies have been developed,such as surface pre-depressurisation and segmented hydraulic fracturing of coal seam roof strata.Additionally,techniques like large-scale volume fracturing in horizontal wells have been established,significantly enhancing reservoir stimulation effects and coalbed methane recovery rates.However,all of these technologies are fundamentally based on permeation.These technologies lack direct methods aimed at enhancing the diffusion rate of CBM,thereby failing to fully reflect the unique characteristics of CBM.Current CBM exploration and development theories and technologies are not universally applicable to all coal seams.They do not adequately account for the predominantly adsorbed state of CBM,and the complex and variable gas generation mechanisms further constrain CBM development in China.Finally,continuous exploration of new deep CBM exploration technologies is necessary.Integrating more effective reservoir stimulation technologies is essential to enhance technical adaptability concerning CBM reservoir characteristics,gas occurrence states,and gas generation mechanisms,ultimately achieving efficient CBM development.We conclude that while China possesses a substantial foundation of deep fractured CBM resources,industry development is constrained and requires continuous exploration of new CBM exploration and development technologies to utilize these resources effectively.展开更多
The organotypic retinal explant culture has been established for more than a decade and offers a range of unique advantages compared with in vivo experiments and cell cultures.However,the lack of systematic and contin...The organotypic retinal explant culture has been established for more than a decade and offers a range of unique advantages compared with in vivo experiments and cell cultures.However,the lack of systematic and continuous comparison between in vivo retinal development and the organotypic retinal explant culture makes this model controversial in postnatal retinal development studies.Thus,we aimed to verify the feasibility of using this model for postnatal retinal development studies by comparing it with the in vivo retina.In this study,we showed that postnatal retinal explants undergo normal development,and exhibit a consistent structure and timeline with retinas in vivo.Initially,we used SOX2 and PAX6 immunostaining to identify retinal progenitor cells.We then examined cell proliferation and migration by immunostaining with Ki-67 and doublecortin,respectively.Ki-67-and doublecortin-positive cells decreased in both in vivo and explants during postnatal retinogenesis,and exhibited a high degree of similarity in abundance and distribution between groups.Additionally,we used Ceh-10 homeodomain-containing homolog,glutamate-ammonia ligase(glutamine synthetase),neuronal nuclei,and ionized calcium-binding adapter molecule 1 immunostaining to examine the emergence of bipolar cells,Müller glia,mature neurons,and microglia,respectively.The timing and spatial patterns of the emergence of these cell types were remarkably consistent between in vivo and explant retinas.Our study showed that the organotypic retinal explant culture model had a high degree of consistency with the progression of in vivo early postnatal retina development.The findings confirm the accuracy and credibility of this model and support its use for long-term,systematic,and continuous observation.展开更多
The reform stems from honesty and determination. Since 2005, organ donation and transplantation in China has undergone thorough reform, which complies with legislation requirements and ethical principles established b...The reform stems from honesty and determination. Since 2005, organ donation and transplantation in China has undergone thorough reform, which complies with legislation requirements and ethical principles established by the World Health Organization(WHO). Reform in China has demonstrated the unwavering confidence and utmost determination of the Chinese government and the Chinese transplantation community. The year 2015 marked a historic turning point when voluntary donations from Chinese citizens became the sole legitimate source for organ transplantation. Since 2015, China has gradually established and refined the “Chinese Mode” and “China System” for organ donation and transplantation, fulfilling its political pledge of reform, and has garnered international recognition, and fostered a social culture which promotes organ donation. This article reviewed the history of reform on organ donation and transplantation in China, presented a new pattern of establishment of organ donation system in the new era of the country, and the direction of advances in the future.展开更多
Phenanthrene(Phe)is one of the common polycyclic aromatic hydrocarbons in the environment,and recent studies show that it can cause cardiac developmental toxicity and immunotoxicity.However,it is still unknown whether...Phenanthrene(Phe)is one of the common polycyclic aromatic hydrocarbons in the environment,and recent studies show that it can cause cardiac developmental toxicity and immunotoxicity.However,it is still unknown whether it can affect the hematopoietic development in aquatic organisms.To address this question,zebrafish(Danio rerio)were chronically exposed to Phe at different concentrations.We found that Phe caused structural damage to the renal tubules in the kidney,induced malformed erythrocytes in peripheral blood,and decreased the proportion of myeloid cells in adult zebrafish,suggesting possible negative impacts that Phe posed to hematopoietic development.Then,using in situ hybridization technology,we found that Phe decreased the expression of primitive hematopoietic marker genes,specifically gata1 and pu.1,accompanied by an obstruction of primitive erythrocyte circulation.Furthermore,Phe impaired definitive hematopoiesis,increased aberrations of the transient hematopoietic site(PBI),and reduced the generation of hematopoietic stem cells,ultimately influencing the number of erythrocytes and myeloid cells.The findings suggested that Phe could induce hematopoietic toxicity in zebrafish embryos and pose unknown ecological risks.展开更多
基金supported by the National Key Research and Development Program of China(2023YFD2301500).
文摘A morphology-based growth stage system should describe the growth and development of a crop and thereby help farmers and agronomists in formulating reasonable managementmeasures conducive to the development of marketable products.However,existing growth stage systems for soybean are either based on plant growth or covered particular phases of flower or pod development,making it difficult to use for tracking the entire growth period of individual flowers and pods.Therefore,the first flower and pod,located at the base of the primary raceme in the eighth trifoliate node of the main stem,were chosen to illustrate growth dynamics during the full reproductive period.The size and fresh weight of the primary raceme in the eighth trifoliate leaf axil,the first flower and pod,the pistils in the first flower,and seeds in the first pod were examined,and the growth of these organs was depicted.Integrating the morphological characteristics and growth features of flowers and pods,as well as existing growth stage systems,the growth and development were delineated in 13 stages.In detail,we classified the flower phase based on the relative positions of floral components,inspired by the ratio of bract to flower used for staging,refined the lag phase proposed previously,retained the use of pod length to define the early pod phase,and innovatively described the late pod phase by the seed appearance.The developmental events in each stage of flower and pod were distinctive and closely connected to the corresponding morphology.Taken together,a more detailed growth stage system for describing individual flowers and pods in soybean was established.This system will serve as a valuable research tool for describing the development,gene expression,and cellular metabolism associated with the formation of flowers,pods,and seeds.
基金2024 Annual Project of Philosophy and Social Sciences Planning of Shenzhen(Project No.:SZ2024B011)2025 Special Research Project of Shenzhen Academy of Social Sciences:Institutional Mechanisms for Promoting Deep Integration Between Manufacturing and Digital Economy in Shenzhen,School-level Quality Engineering Project of Shenzhen Polytechnic(Project No.:10018021310011Z)School-level Scientific Research Project of Shenzhen Polytechnic(Project No.:6025310012S)。
文摘Economic growth and fair distribution are two major goals pursued in economic development.Through a comparative analysis of economies such as those in Europe and the United States,it is found that:Economic growth does not have to be conditional on the expansion of income gap;growth will not automatically lead to fair distribution;efficiency and fairness can occur simultaneously;the transformation of the economic development mode can be achieved through income distribution.A shared development model that takes into account both growth and fairness is the choice to achieve sustained and stable economic growth.It is necessary to deepen the reform of the income distribution system,improve social security and other measures to promote the development achievements to benefit the majority of workers more,and achieve both efficiency and fairness at the same time.
文摘Nowadays,with the continuous deepening of quality-oriented education in China and the increasing demands of parents,especially the implementation of the new curriculum design concept,tremendous changes have taken place in teaching methods,teaching contents,teaching means,and learning approaches,which pose new challenges to the professional growth of preschool teachers.At present,preschool teachers are not only required to have a sense of responsibility and love,but also to have rich professional knowledge and skills,as well as new creative thinking and research ability.Even if the preschool teachers who have performed very well in traditional education are now struggling to adapt to the actual needs of educational development,which requires preschool teachers to continuously develop in the direction of specialization.This article will focus on how to promote the professional growth and development of preschool teachers to analyze and elaborate.
文摘BACKGROUND Newborns with low-birth-weight may lag behind those with normal-birth-weight in terms of growth,development,and nutritional status,which increases mothers’concerns about the child’s future health and leads to anxiety and depression.Providing nutritional support to newborns with low-birth-weight to facilitate optimal growth and development may help alleviate maternal anxiety and depre-ssion.RESULTS No significant difference in baseline data was observed between the two groups(P>0.05).Milk intake and TBIL levels in Group S were significantly better than those in Group L at 1,2,3,and 4 weeks after nutritional support(P<0.05).No significant differences in vomiting,abdominal distension,constipation,diarrhea,and residual adverse reactions were observed between the two groups during nutritional support(P>0.05).Group S had a significantly reduce of fewer complications(i.e.,neonatal necrotizing enterocolitis and infection)than did Group L during nutritional support(P<0.05).Before nutritional support,no significant differences in developmental indicators(weight,length,and head circumference)were noted between the two groups(P>0.05).However,after nutritional support,the weight,length,and head circumference of both groups were higher than those before intervention,with Group S having significantly higher values than did Group L(P<0.05).Mothers of children who received nutritional support had decreased SAS and SDS scores,with Group S mothers having significantly lower scores than did Group L mothers(P<0.05).CONCLUSION Enteral nutrition support had significant effects on newborns weighing<2 kg within 24 hours of birth.In particular,it promoted the maturation of gastrointestinal function,enhanced nutrient absorption,promoted optimal short-term growth and development,and alleviated the mother’s anxiety and depression.
文摘BACKGROUND Unintended pregnancy occurs when an individual or couple conceives without planning or desire,which can potentially affect a child’s physical,mental,and social well-being.This can then lead to long-term socioeconomic challenges for families and communities.Although its impact on child growth and development is a pressing concern,research remains limited particularly in multicenter settings.AIM To examine the long-term consequences of unintended pregnancy on the critical years of early childhood growth and development.METHODS This analytical observational study employed a case-control design and was conducted in research centers across Indonesia,encompassing those located in Central Java,Lampung,Bali,and West Nusa Tenggara.A total of 700 children aged≤5 years with histories of intended or unintended pregnancies participated.Data collection involved structured interviews and direct anthropometric and developmental assessments.Data analyses were conducted using multivariate statistics and partial least squares structural equation modeling.RESULTS Unintended pregnancy was found to have a statistically significant effect on both child growth(t=8.178;P<0.001)and child development(t=25.688;P<0.001).Key growth problems identified included underweight,undernutrition,abnormal head circumference,and stunting.Developmental challenges prominently associated with unintended pregnancy included behavioral and emotional disorders,autism spectrum disorder,attentiondeficit/hyperactivity disorder,social and motor skill deficits,as well as visual and hearing impairments.CONCLUSION Unintended pregnancy significantly affects child growth and development,underscoring the need for early intervention,quality prenatal care,and strengthened family planning policies.
基金Supported by 2024 Academy Level Research Start up Fund,No.YK202434.
文摘BACKGROUND First-time mothers may encounter various problems during postpartum,which can result in negative emotions that can affect infant care.In today’s Internet era,continuous nursing services can be provided to mothers and their babies after delivery through Internet-based platforms.This approach can help reduce negative emotions of primiparas and promote better health for both mothers and babies.AIM To explore the effect of Internet Plus-based postpartum healthcare services on postpartum depression of primiparas and neonatal growth and development and thus provide a scientific basis for strengthening postpartum healthcare measures and better protect maternal and child health.METHODS The study retrospectively collected data of primiparas and their newborns who underwent prenatal examination and successfully delivered at the Ninth People’s Hospital of Suzhou City.The observation group included 30 primiparas and their newborns who received Internet Plus-based postpartum healthcare services between July and December 2024.According to the principle of matching(1:1)control study,the control group included 30 primiparas and their newborns who received routine postpartum healthcare services between January and June 2024.The maternal role adaptation questionnaire scores,breastfeeding rates,Edinburgh postnatal depression scale(EPDS)scores,and newborn growth and development(height,head circumference,and weight)were compared between the two groups at the time of discharge after delivery and 6-week postpartum follow-up.RESULTS Upon hospital discharge,the two groups did not demonstrate significant differences in maternal role adaptation scores,breastfeeding rates,EPDS scores,as well as newborn height,head circumference,and weight at birth(P>0.05).At the 6-week postpartum follow-up,the maternal role adaptation score and breastfeeding rate were higher in the observation group than in the control group(P<0.05).In addition,one case of postpartum depression was reported in the observation group and eight in the control group.Moreover,the control group exhibited a significant increase in EPDS scores compared with scores at hospital discharge(P<0.05),whereas the observation group showed only a marginal,nonsignificant increase in EPDS scores(P>0.05).The EPDS score of the observation group was significantly lower than that of the control group(P<0.05),indicating a lower risk of postpartum depression in the observation group.The length,head circumference,and weight of the newborns 6 weeks after birth were increased compared with those at birth,and the growth rate was higher in the observation group than in the control group(P<0.05),indicating better growth and development in the observation group.CONCLUSION Internet Plus-based postpartum healthcare services improve maternal role adaptation,increase breastfeeding rates,mitigate postpartum depression risk,and promote neonatal growth and development in primiparas.
基金supported by the Key Projects in Science and Technology of Inner Mongolia,China(2021ZD0031)the China Agriculture Research System of MOF and MARA(CARS-34)+1 种基金the Performance Incentive and Guidance Special Project of Scientific Research Institution,Chongqing Science and Technology Committee,China(cstc2022jxjl80019)the Natural Science Foundation of Chongqing,China(CSTB2022NSCQ-BHX0744)。
文摘Related to ABI3 and VP1(RAV)transcription factors belong to the AP2 and B3 superfamily.RAVs genes have been reported to be involved in plant growth and development regulation.This study screened three RAV genes from Medicago truncatula and named one of them MtRAV1.The MtRAV1 overexpressing plants exhibits traits such as plant dwarfing,delayed flowering,reduced leaf and floral organs,increased branching,and reduced pods and seeds.Gene expression analysis results showed that overexpression of Mt RAV1 inhibited the expression of Flowering Locus T(MtFTa1),Suppressor of Overexpression of CO1(MtSOC1),GA3-oxidase1(MtGA3OX1),DWARF14(MtD14)and Carotenoid Cleavage Dioxygenase7(MtCCD7).To further investigate the regulation pathway involved by MtRAV1,RNA-sequencing(RNA-seq)and DNA affinity purification sequencing(DAP-seq)analysis were conducted.RNA-seq results indicated that MtRAV1 might affect plant growth and development by regulating some genes in photosynthesis,circadian rhythm and plant hormone signaling pathways,especially the auxin signaling pathway.Conjoint analysis of DAP-seq and RNA-seq revealed that Mt RAV1 might inhibit the expression of Ferredoxin(Mt Fd-l3),Light-harvesting Chlorophyll a/b Binding Protein 1(Mt Lhcb-l2)and Small Auxin Up-regulated RNA(Mt SAUR-l),which related to photosystem II and auxin signaling pathway.Summarily,MtRAV1 was preliminarily proven to be a key growth inhibitory factor in M.truncatula.
基金Supported by China Agriculture(Cotton)Research System(CARS-15-46)Intellectual Aid Xinjiang Innovation and Expansion Talent Program of Xinjiang Uygur Autonomous Region(2024500207).
文摘[Objectives]To provide a reference for the promotion of appropriate row spacing configuration modes for cotton planting in the Bortala Mongol Autonomous Prefecture of Xinjiang.[Methods]Xinluzao 63 was employed as the research subject to examine the effects of three different configuration modes:three rows with one film,four rows with one film,and six rows with one film,on the growth and development of cotton,as well as on yield and the amount of residual film in the field.[Results]In comparison to the configuration modes of four rows with one film and six rows with one film,the development process in the row spacing configuration mode of three rows with one film was accelerated by 1-4 d.This configuration mode exhibited variability in several agronomic traits,particularly in plant height,the number of fruiting branches per plant,and the number of leaves per plant,with the observed trend indicating T3>T2>T1.Conversely,the height of the first fruiting branch node displayed an inverse trend.In terms of yield composition,no significant differences were observed in boll weight and yield among various configuration modes.However,T3 exhibited the highest boll weight at 5.68 g and a yield of 462.67 kg/667 m 2.Additionally,significant differences were noted in harvesting density and the number of bolls per plant.T3 demonstrated the lowest harvesting density at 1.11×104 plants/666.7 m 2,the highest number of bolls per plant at 8.63,and the highest boll opening rate at 97.48%.Furthermore,T3 also resulted in the least amount of agricultural film residue during the current season.[Conclusions]Among the three planting configuration modes examined,the low-density planting configuration mode consisting of three rows and one film demonstrated a significant advantage at the individual plant level.This approach yielded results comparable to those of the high density planting configuration mode while also reducing costs.Furthermore,low density planting positively influenced the cotton boll opening rate,leading to a decreased amount of residual film and promoting ecological health within the agricultural land.
文摘Objective:To explore the positive role of developmental care measures in promoting the growth and development of premature infants,and analyze their specific effects on improving the sleep duration and quality of premature infants.Methods:A total of 80 premature infants who met the inclusion criteria were selected by convenient sampling method and divided into an experimental group and a control group,with 40 cases in each group.The experimental group received comprehensive nursing intervention of developmental care during the nursing process;the control group adopted the traditional conventional nursing model.Results:There was no statistically significant difference in various physical indicators between the two groups of premature infants before nursing intervention.After nursing intervention,the scores of intellectual development level,psychomotor development index and various physical indicators of premature infants in the experimental group were significantly higher than those in the control group,and the difference was statistically significant.Conclusion:The implementation of developmental care for premature infants can significantly improve their various developmental indicators,which can not only effectively promote the rapid development of premature infants’mental and physical growth,but also improve their sleep quality.
基金the Key Research and Development Project of Ningxia Hui Autonomous Region(No.2023BEG02072)for their financial support.
文摘Repeated wet swelling and dry shrinkage of soil leads to the gradual occurrence of cracks and the formation of a complex fracture network.In order to study the development characteristics and quantitative analysis of cracks in root-soil complex in different growth periods under dry-wet cycles,the alfalfa root-loess complex was in-vestigated during different growth periods under different dry-wet cycles,and a dry-wet cycle experiment was conducted.The crack rate,relative area,average width,total length,and the cracks fractal dimension in the root-soil complex were extracted;the crack development characteristics of plain soil were analyzed under the PG-DwC(dry-wet cycle caused by plant water management during plant growth period),as well as the crack development characteristics of root-soil complex under PG-DWC and EC-DWC(the dry-wet cycles caused by extreme natural conditions such as continuous rain);the effects of plant roots and dry-wet cycles on soil cracks were discussed.The results showed that the average crack width,crack rate,relative crack area,and total crack length of the alfalfa root-loess complex were higher than those of the plain soil during PG-DWC.The result indicated that compared with plain soil during PG-DWC,the presence of plant roots in alfalfa root-soil complex in the same growth period promoted the cracks development to some extent.The alfalfa root-soil complex crack parameters during different growth periods were relatively stable during PG-DWC(O dry-wet cycle).During EC-DWC(1,3,and 5 dry-wet cycles),the alfalfa root-loess complex crack parameters increased with the number of dry-wet cycles during different growth periods.Unlike PG-DWC,the EC-DWC accelerated crack development,and the degree of crack development increased with the number of dry-wet cycles.The existence of plant roots promoted crack development and expansion in the root-soil complex to a certain extent,and the dry-wet cycle certainly promoted crack development and expansion in the root-soil complex.This result contradicts the im-provement in the root-soil complex's macro-mechanical properties during plant growth,due to differences in the mechanical properties of roots and soil.The research results will provide reference for the root soil complex crack development law and the design of slope protection by vegetation.
基金The financial support from the National Key Research and Development Program of China(No.2023YFD1700600)。
文摘Agrochemicals,especially plant growth regulators(PGRs),are extensively used to modulate endogenous phytohormone signals in small quantities,significantly infiuencing plant growth and development.Plant hormones typically exhibit diverse chemical structures,with common examples including indole rings,terpenoid frameworks,adenine motifs,cyclic lactones,cyclopentanones,and steroidal compounds,which are extensively employed in pesticides.This article explores the interactions and biological activities of small molecules on proteins,enzymes,and other reactive sites involved in the biosynthesis,metabolism,transport,and signal transduction pathways of various plant hormones.Additionally,it analyzes the structure-activity relationships(SARs)of pesticides incorporating these structural motifs to elucidate the relationship between active fragments,pharmacophores,and targets,highlighting the characteristics of potent small molecules and their derivatives.This comprehensive review aims to provide novel perspectives for the development and design of pesticides,offering valuable insights for researchers in the field.
文摘In the Turkish Economy,there were radical changes in the structure of the economy with the policies of opening up to the outside world and transition to a free market economy in the 1980s,and the last step of this opening up and liberalization process was realized with the decision number 32 in 1989.We can say that with the liberalization of capital movements in the 1990s,economic growth and development were tried to be achieved through hot money inflows rather than direct foreign investments.This trend made the economy more open to crises,and for the first time,a crisis occurred in the form of the 1994 economic crisis,which was understood to be caused by hot money.The same economic structure experienced a financial and economic crisis caused by hot money again in November 2000 and February 2001.While the crisis was overcome with the stand-by agreement made with the IMF(International Monetary Fund)and the announced Transition to a Strong Economy program,the economy started to grow rapidly with the ease of using foreign resources,and political stability seems to have enabled this economic growth process to continue uninterruptedly except 2009.There was also a decrease in inflation rates.The same economic structure continued in the period between 2010 and 2020,and the financing need of economic growth was met by outsourcing.However,this process was different from the previous decade and there was no economic and financial crisis other than the sudden increase in exchange rates in 2018.We can say that the sudden exchange rate increase in 2018 was perceived as a harbinger of possible exchange rate shocks in the following years.
基金the National Key Research and Development Program of China(2022YFD1500705)National Natural Science Foundation of China(U19A2035)for financial support。
文摘Strip-till(ST),including straw mulching in the inter-row and localized fertilization in the intra-row,is a conservation tillage system for improving soil quality and crop growth.However,the yield advantage of maize under ST compared to conventional tillage(CT)remains unstable,and the strategies to increase maize yield under ST are unclear.This study aims to understand the physiological mechanism underlining maize yield formation under ST by comparing two maize cultivars,DKM753 and DK517,with contrasting yield performance in ST versus CT systems.Compared to CT,ST resulted in a 4.5%yield increase for DKM753 but a 5.6%decrease for DK517.These yield differences were primarily attributed to variations in grain number per ear(GN).During the rapid growth stage(V14-R3),i.e.,two weeks before and after silking,DKM753 showed a 6.7%increase in maximum growth rate(V_(max))and a 6.3%increase in average growth rate(V)under ST,whereas DK517 exhibited decline of 8.5%in V_(max) and 12.3%in V.Significant positive correlations are observed between V_(max) and V with GN under ST(R^(2)=0.79 and R^(2)=0.90,respectively).Enhanced dry matter accumulation in DKM753 under ST was attributed to increased leaf expansion rates,contributing to a larger photosynthate source.The straw mulching and localized nitrogen fertilization increased root-zone nitrogen availability at silking in ST compared to CT.DKM753 had a greater root system which made better use of the soil N and lead to an increased leaf nitrogen accumulation by 14.9%under ST.It is concluded that maize yield under the strip-till system is determined by grain number per ear,which can be increased by increasing nitrogen accumulation,plant growth,and ear development around silking stage.A sound root system can efficiently utilize soil nitrogen resources under the strip-till system,increasing plant nitrogen accumulation and thereby promoting plant growth.
基金Financial support by Natural Science Foundation of China(52430001)is acknowledged.
文摘We developed a strategy involving an electroactive biofiltration dynamic membrane(EBDM)for wastewater treatment and membrane fouling mitigation.This approach utilizes a cathode potential within an anaerobic dynamic membrane bioreactor to establish a growth equilibrium electroactive fouling layer.Over a 240 day operation period,the EBDM exhibited outstanding performance,characterized by an ultralow fouling rate(transmembrane pressure<2.5 kPa),superior effluent quality(chemical oxygen demand(COD)removal>93%and turbidity 2 nephelometric turbidity units(NTU)),and a 7.2%increase in methane(CH4)productivity.Morphological analysis revealed that the EBDM acted as a biofilter consisting of a structured,interconnected,multilevel dynamic membrane system with orderly clogging.In the EBDM system,the balanced-growth fouling layers presented fewer biofoulants and looser secondary protein structures.Furthermore,the applied electric field modified the physicochemical properties of the biomass,leading to a decrease in fouling potential.Quartz crystal microbalance with dissipation monitoring analysis indicated that growth equilibrium promoted a looser fouling layer with a lower adsorption mass than did the denser,viscoelastic fouling layer observed in the control reactor.Metagenomic sequencing further demonstrated that continuous electrical stimulation encouraged the development of an electroactive fouling layer with enhanced microbial metabolic functionality on the EBDM.This approach selectively modifies metabolic pathways and increases the degradation of foulants.The EBDM strategy successfully established an ordered-clogging,step-filtered,and balanced-growth electroactive fouling layer,achieving a synergistic effect in reducing membrane fouling,enhancing effluent quality,and improving CH_(4)productivity.
基金supported by the National Key R&D Program of China,No.2019YFA0110300(to ZG)the National Natural Science Foundation of China,Nos.81773302(to YF),32070862(to ZG).
文摘Human brain development is a complex process,and animal models often have significant limitations.To address this,researchers have developed pluripotent stem cell-derived three-dimensional structures,known as brain-like organoids,to more accurately model early human brain development and disease.To enable more consistent and intuitive reproduction of early brain development,in this study,we incorporated forebrain organoid culture technology into the traditional unguided method of brain organoid culture.This involved embedding organoids in matrigel for only 7 days during the rapid expansion phase of the neural epithelium and then removing them from the matrigel for further cultivation,resulting in a new type of human brain organoid system.This cerebral organoid system replicated the temporospatial characteristics of early human brain development,including neuroepithelium derivation,neural progenitor cell production and maintenance,neuron differentiation and migration,and cortical layer patterning and formation,providing more consistent and reproducible organoids for developmental modeling and toxicology testing.As a proof of concept,we applied the heavy metal cadmium to this newly improved organoid system to test whether it could be used to evaluate the neurotoxicity of environmental toxins.Brain organoids exposed to cadmium for 7 or 14 days manifested severe damage and abnormalities in their neurodevelopmental patterns,including bursts of cortical cell death and premature differentiation.Cadmium exposure caused progressive depletion of neural progenitor cells and loss of organoid integrity,accompanied by compensatory cell proliferation at ectopic locations.The convenience,flexibility,and controllability of this newly developed organoid platform make it a powerful and affordable alternative to animal models for use in neurodevelopmental,neurological,and neurotoxicological studies.
基金supported by the National Natural Science Foundation of China(52074045,52274074)the Science Fund for Distinguished Young Scholars of Chongqing(CSTB2022NSCQ-JQX0028).
文摘Developing deep fragmented soft coalbed methane(CBM)can significantly enhance domestic natural gas supplies,reduce reliance on imported energy,and bolster national energy security.This manuscript provides a comprehensive review of commonly employed coalbed methane extraction technologies.It then delves into several critical issues in the current stage of CBM exploration and development in China,including the compatibility of existing technologies with CBM reservoirs,the characteristics and occurrence states of CBM reservoirs,critical desorption pressure,and gas generation mechanisms.Our research indicates that current CBM exploration and development technologies in China have reached an internationally advanced level,yet the industry is facing unprecedented challenges.Despite progress in low-permeability,high-value coal seams,significant breakthroughs have not been achieved in exploring other types of coal seams.For different coal reservoirs,integrated extraction technologies have been developed,such as surface pre-depressurisation and segmented hydraulic fracturing of coal seam roof strata.Additionally,techniques like large-scale volume fracturing in horizontal wells have been established,significantly enhancing reservoir stimulation effects and coalbed methane recovery rates.However,all of these technologies are fundamentally based on permeation.These technologies lack direct methods aimed at enhancing the diffusion rate of CBM,thereby failing to fully reflect the unique characteristics of CBM.Current CBM exploration and development theories and technologies are not universally applicable to all coal seams.They do not adequately account for the predominantly adsorbed state of CBM,and the complex and variable gas generation mechanisms further constrain CBM development in China.Finally,continuous exploration of new deep CBM exploration technologies is necessary.Integrating more effective reservoir stimulation technologies is essential to enhance technical adaptability concerning CBM reservoir characteristics,gas occurrence states,and gas generation mechanisms,ultimately achieving efficient CBM development.We conclude that while China possesses a substantial foundation of deep fractured CBM resources,industry development is constrained and requires continuous exploration of new CBM exploration and development technologies to utilize these resources effectively.
基金supported by the National Natural Science Foundation of China,Nos.81901156(to ZZ),82271200(to ZZ),82171308(to XC)the Fundamental Research Funds for the Central Universities,No.xzy012022035(to ZZ)+1 种基金the Natural Science Foundation of Shaanxi Province,Nos.2021JM-261(to QK),2023-YBSF-303(to ZZ)Traditional Chinese Medicine Project of Shaanxi Province,No.2019-ZZ-JC047(to QK)。
文摘The organotypic retinal explant culture has been established for more than a decade and offers a range of unique advantages compared with in vivo experiments and cell cultures.However,the lack of systematic and continuous comparison between in vivo retinal development and the organotypic retinal explant culture makes this model controversial in postnatal retinal development studies.Thus,we aimed to verify the feasibility of using this model for postnatal retinal development studies by comparing it with the in vivo retina.In this study,we showed that postnatal retinal explants undergo normal development,and exhibit a consistent structure and timeline with retinas in vivo.Initially,we used SOX2 and PAX6 immunostaining to identify retinal progenitor cells.We then examined cell proliferation and migration by immunostaining with Ki-67 and doublecortin,respectively.Ki-67-and doublecortin-positive cells decreased in both in vivo and explants during postnatal retinogenesis,and exhibited a high degree of similarity in abundance and distribution between groups.Additionally,we used Ceh-10 homeodomain-containing homolog,glutamate-ammonia ligase(glutamine synthetase),neuronal nuclei,and ionized calcium-binding adapter molecule 1 immunostaining to examine the emergence of bipolar cells,Müller glia,mature neurons,and microglia,respectively.The timing and spatial patterns of the emergence of these cell types were remarkably consistent between in vivo and explant retinas.Our study showed that the organotypic retinal explant culture model had a high degree of consistency with the progression of in vivo early postnatal retina development.The findings confirm the accuracy and credibility of this model and support its use for long-term,systematic,and continuous observation.
文摘The reform stems from honesty and determination. Since 2005, organ donation and transplantation in China has undergone thorough reform, which complies with legislation requirements and ethical principles established by the World Health Organization(WHO). Reform in China has demonstrated the unwavering confidence and utmost determination of the Chinese government and the Chinese transplantation community. The year 2015 marked a historic turning point when voluntary donations from Chinese citizens became the sole legitimate source for organ transplantation. Since 2015, China has gradually established and refined the “Chinese Mode” and “China System” for organ donation and transplantation, fulfilling its political pledge of reform, and has garnered international recognition, and fostered a social culture which promotes organ donation. This article reviewed the history of reform on organ donation and transplantation in China, presented a new pattern of establishment of organ donation system in the new era of the country, and the direction of advances in the future.
基金supported by the National Natural Science Foundation of China(Nos.22276117 and 22076108)the Science and Technology Innovation Talent Team Project of Shanxi Province(No.202204051002024).
文摘Phenanthrene(Phe)is one of the common polycyclic aromatic hydrocarbons in the environment,and recent studies show that it can cause cardiac developmental toxicity and immunotoxicity.However,it is still unknown whether it can affect the hematopoietic development in aquatic organisms.To address this question,zebrafish(Danio rerio)were chronically exposed to Phe at different concentrations.We found that Phe caused structural damage to the renal tubules in the kidney,induced malformed erythrocytes in peripheral blood,and decreased the proportion of myeloid cells in adult zebrafish,suggesting possible negative impacts that Phe posed to hematopoietic development.Then,using in situ hybridization technology,we found that Phe decreased the expression of primitive hematopoietic marker genes,specifically gata1 and pu.1,accompanied by an obstruction of primitive erythrocyte circulation.Furthermore,Phe impaired definitive hematopoiesis,increased aberrations of the transient hematopoietic site(PBI),and reduced the generation of hematopoietic stem cells,ultimately influencing the number of erythrocytes and myeloid cells.The findings suggested that Phe could induce hematopoietic toxicity in zebrafish embryos and pose unknown ecological risks.
