In this project,we aim to elucidate the molecular mechanism controlling initiation and elongation of tetraploid Gossypium hirsutum fiber cells by setting up a high throughput custom-designed cDNA microarray and a syst...In this project,we aim to elucidate the molecular mechanism controlling initiation and elongation of tetraploid Gossypium hirsutum fiber cells by setting up a high throughput custom-designed cDNA microarray and a systematic gene expression profiling during cotton fiber development.We first con-structed a microarray consisting of more than 28,000 cotton UniESTs that we obtained by deep-se-quencing of several cotton ovule cDNA libraries.After probing the microarray with RNA samples from wild-type and a fuzzless-lintless mutant at different develop mental stages,we identified ca.1000 genes so mewhat involved in fiber initiation and elongation processes.展开更多
The use of a compact disk （CD） pickup head as a displacement measurement system is described. The components contained in a pickup head are explained and how they are combined to obtain the functionality of a pickup...The use of a compact disk （CD） pickup head as a displacement measurement system is described. The components contained in a pickup head are explained and how they are combined to obtain the functionality of a pickup head. The application of measuring a knife edge profile is introduced. The results reveal some insuffiencies with the current system. The cutting edge＇s radius of curvature can be estimated.展开更多
文摘In this project,we aim to elucidate the molecular mechanism controlling initiation and elongation of tetraploid Gossypium hirsutum fiber cells by setting up a high throughput custom-designed cDNA microarray and a systematic gene expression profiling during cotton fiber development.We first con-structed a microarray consisting of more than 28,000 cotton UniESTs that we obtained by deep-se-quencing of several cotton ovule cDNA libraries.After probing the microarray with RNA samples from wild-type and a fuzzless-lintless mutant at different develop mental stages,we identified ca.1000 genes so mewhat involved in fiber initiation and elongation processes.
基金the partial financial support for the work presented in this paper by the Austrian Research Promotion Agencythe Austrian COMET program supporting the Austrian Center of Competence in Mechatronics (ACCM)
文摘The use of a compact disk （CD） pickup head as a displacement measurement system is described. The components contained in a pickup head are explained and how they are combined to obtain the functionality of a pickup head. The application of measuring a knife edge profile is introduced. The results reveal some insuffiencies with the current system. The cutting edge＇s radius of curvature can be estimated.
