Through embryo culture, intergeneric hybrids were produced between common wheat,Triticum aestivum cv. Chinese Spring (CS) (2n= 6x = 42, AABBDD) and crested wheatgrass,Agropyron desertorum (2n =4x =28, PPPP) for the fi...Through embryo culture, intergeneric hybrids were produced between common wheat,Triticum aestivum cv. Chinese Spring (CS) (2n= 6x = 42, AABBDD) and crested wheatgrass,Agropyron desertorum (2n =4x =28, PPPP) for the first time. Their F_2 and BC_1 seeds havealso been successfully obtained by selfing and backcrossing with wheat in F_1 hybrids. Theseedlings of all F_1 hybrids tended to resemble their female parent, but they had more tillers,and their spike characteristics were between their parents. Average meiotic chromosomepairing at MI of F_1 hybrids was: 6.62Ⅰ+ 8.20RingⅡ+ 4.16RodⅡ+0.57Ⅲ+ 0.35Ⅳ+0.06Ⅴ+0.03Ⅵ. The successful experience of hybridization of common wheat with A. desertorum,the reason for the high level of chromosome pairing and self-fertility in the F_1 hybrids werediscussed. The F_2 and BC_1 seeds obtained in F_1 hybrids are of great importance to theory andpractice.展开更多
文摘Through embryo culture, intergeneric hybrids were produced between common wheat,Triticum aestivum cv. Chinese Spring (CS) (2n= 6x = 42, AABBDD) and crested wheatgrass,Agropyron desertorum (2n =4x =28, PPPP) for the first time. Their F_2 and BC_1 seeds havealso been successfully obtained by selfing and backcrossing with wheat in F_1 hybrids. Theseedlings of all F_1 hybrids tended to resemble their female parent, but they had more tillers,and their spike characteristics were between their parents. Average meiotic chromosomepairing at MI of F_1 hybrids was: 6.62Ⅰ+ 8.20RingⅡ+ 4.16RodⅡ+0.57Ⅲ+ 0.35Ⅳ+0.06Ⅴ+0.03Ⅵ. The successful experience of hybridization of common wheat with A. desertorum,the reason for the high level of chromosome pairing and self-fertility in the F_1 hybrids werediscussed. The F_2 and BC_1 seeds obtained in F_1 hybrids are of great importance to theory andpractice.
文摘耐旱苔藓植物常常单个个体矮小、生物量低,如何从细小的单个个体中有效提取总DNA是进一步开展居群遗传多样性研究的关键。本研究以古尔班通古特沙漠广泛分布的刺叶墙藓(Tortula desertorum)为对象,使用快速提取法、2×CTAB法及DNeasy plant mini kit试剂盒提取法等3种方法对刺叶墙藓单个个体的总DNA进行提取。结果表明,2×CTAB法提取的DNA纯度高,凝胶电泳显示无明显降解现象,适宜作为PCR扩增的模板。利用所提取的单个个体DNA为模板,建立了优化的RAPDI、SSR反应体系。
