Objective:To evaluate in vitro antioxidant and apoptotic activities of Crperus rotundas(C.rotundus).Methods:The phytochemical study and the antioxidant activities of both methanol and aqueous extracts from C.rotundus ...Objective:To evaluate in vitro antioxidant and apoptotic activities of Crperus rotundas(C.rotundus).Methods:The phytochemical study and the antioxidant activities of both methanol and aqueous extracts from C.rotundus aerial part were determined.In addition,these extracts were also investigated for their cytotoxic and apoptotic activities.The major compound of the methanol extract was isolated.Both metlianol and aqueous extracts(300,150,and 50μg/mL)were evaluated for their antioxidant activity by the xanthine/xanthine oxidase assay system.However,16,8,and 4 mg/mL of each extract were tested to investigate their OH.formation scavenging potential.Aqueous extract(800,400.and 200μg/mL)and melhunol extract(350,175,and 88μg/mL)were tested against lipid peroxidation,induced by 75μM H_2O_2,The cytotoxicity(by MTT assay)and cell DNA fragmentation of both extracts were evaluated Inwards K562 and L1210 cell lines.The major compound was obtained from the butanol fraction of methanol extract and its structure was determined by KMN spectroscopic analysis.Results:The methanol and aqueous extracts showed respectively,88%and 19%inhibition of xanthine oxidase activity.Vet.the same extracts inhibited lipid peroxidation by 61.5%and 42.0%.respectively.Roth extracts inhibited OH.formation by 27.1%and 25.3%,respectively.Only methanol cxtract induced DNA degradation.Orientin was determined as the major compound isolated from the butanol fraction of metlianol extract.Conclusions:It appears that C.rotundus extracts exhibit a potential use as a natural antioxidant and an apoptosis inducer.展开更多
Hiding data in acid （DNA） can facilitate annotation of important plant the deoxyribose nucleic the authentication and variety rights. A grant of plant variety rights for a new plant variety gives you the exclusive r...Hiding data in acid （DNA） can facilitate annotation of important plant the deoxyribose nucleic the authentication and variety rights. A grant of plant variety rights for a new plant variety gives you the exclusive right to produce for sale and sell propagating material of the variety. Digital watermarking techniques have been proposed for a wide range of applications, including ownership protection, copy control, annotation, and authentication. However, existing data hiding methods for DNA change the functionalities of DNA sequences, which induce morphological changes in biological patterns. This paper proposes a high capacity data hiding scheme for DNA without changing the functionalities of DNA sequences. This scheme adaptively varies the embedding process according to the amount of hidden data. Experimental results show that the proposed scheme gives a significantly improved hiding performance than previous schemes. And the robustness and security issues are also analyzed.展开更多
One of the most interesting mysteries of the universe and of life itself is the ubiquity of the chiral nature of the molecules that form the building blocks of life as we know it.For example,amino acids,which are crit...One of the most interesting mysteries of the universe and of life itself is the ubiquity of the chiral nature of the molecules that form the building blocks of life as we know it.For example,amino acids,which are critical to the formation of proteins,are left-handed while deoxyribose,the sugar molecule essential to DNA,is right-handed.A fascinating aspect of chiral molecules is how a change in the handedness,e.g.,from left-handed to right-handed or vice-versa,could lead to a radically different reaction in a living system or the toxicity of a drug.展开更多
Deoxyribonucleic acid(DNA)is a double-strandedc macromoleculee composed of two antiparallel polynucleotide chains.Each chain is formed by deoxyribose and phosphate as the backbone and forms complementary base pairs th...Deoxyribonucleic acid(DNA)is a double-strandedc macromoleculee composed of two antiparallel polynucleotide chains.Each chain is formed by deoxyribose and phosphate as the backbone and forms complementary base pairs through hydrogen bonds.This structure ensures the stability of DNA and enables thec precise replication of genetic information[1].In biological processes,DNA collaborates with various biomolecules,including proteins,RNAs,small molecules,lipids,and carbohydrates[2].展开更多
基金the "Ministere Francais des Affaires Etrangeres"(Action Integree de Cooperation Inter universitaire Franco-Tunisienne,PHC UTIQUE 07 G0836 PAR)the Ministry of Higher Education.Scientific Research and Technology in Tunisia for the financial assistance
文摘Objective:To evaluate in vitro antioxidant and apoptotic activities of Crperus rotundas(C.rotundus).Methods:The phytochemical study and the antioxidant activities of both methanol and aqueous extracts from C.rotundus aerial part were determined.In addition,these extracts were also investigated for their cytotoxic and apoptotic activities.The major compound of the methanol extract was isolated.Both metlianol and aqueous extracts(300,150,and 50μg/mL)were evaluated for their antioxidant activity by the xanthine/xanthine oxidase assay system.However,16,8,and 4 mg/mL of each extract were tested to investigate their OH.formation scavenging potential.Aqueous extract(800,400.and 200μg/mL)and melhunol extract(350,175,and 88μg/mL)were tested against lipid peroxidation,induced by 75μM H_2O_2,The cytotoxicity(by MTT assay)and cell DNA fragmentation of both extracts were evaluated Inwards K562 and L1210 cell lines.The major compound was obtained from the butanol fraction of methanol extract and its structure was determined by KMN spectroscopic analysis.Results:The methanol and aqueous extracts showed respectively,88%and 19%inhibition of xanthine oxidase activity.Vet.the same extracts inhibited lipid peroxidation by 61.5%and 42.0%.respectively.Roth extracts inhibited OH.formation by 27.1%and 25.3%,respectively.Only methanol cxtract induced DNA degradation.Orientin was determined as the major compound isolated from the butanol fraction of metlianol extract.Conclusions:It appears that C.rotundus extracts exhibit a potential use as a natural antioxidant and an apoptosis inducer.
基金supported by the National Science Council,Taiwan under Grant No.NSC 99-2221-E-468-007,NSC 99-2221-E-024-010,NSC 99-2221-E-468-021,and NSC 99-2632-E-468-001-MY3
文摘Hiding data in acid （DNA） can facilitate annotation of important plant the deoxyribose nucleic the authentication and variety rights. A grant of plant variety rights for a new plant variety gives you the exclusive right to produce for sale and sell propagating material of the variety. Digital watermarking techniques have been proposed for a wide range of applications, including ownership protection, copy control, annotation, and authentication. However, existing data hiding methods for DNA change the functionalities of DNA sequences, which induce morphological changes in biological patterns. This paper proposes a high capacity data hiding scheme for DNA without changing the functionalities of DNA sequences. This scheme adaptively varies the embedding process according to the amount of hidden data. Experimental results show that the proposed scheme gives a significantly improved hiding performance than previous schemes. And the robustness and security issues are also analyzed.
文摘One of the most interesting mysteries of the universe and of life itself is the ubiquity of the chiral nature of the molecules that form the building blocks of life as we know it.For example,amino acids,which are critical to the formation of proteins,are left-handed while deoxyribose,the sugar molecule essential to DNA,is right-handed.A fascinating aspect of chiral molecules is how a change in the handedness,e.g.,from left-handed to right-handed or vice-versa,could lead to a radically different reaction in a living system or the toxicity of a drug.
基金supported by the National Natural Science Foundation of China(No.81970431)the Hunan Provincial Natural Science Foundation of China(Nos.2024JJ9370 and 2025JJ50502)+2 种基金the Postgraduate Scientific Research Innovation Project of Hunan Province(Nos.CX20230992 and CX20240840)the Municipal Financial Subsidy for Shenzhen TCM Key Discipline Constructionthe Flagship Department Construction Project for Integrated Traditional Chinese and Western Medicine.
文摘Deoxyribonucleic acid(DNA)is a double-strandedc macromoleculee composed of two antiparallel polynucleotide chains.Each chain is formed by deoxyribose and phosphate as the backbone and forms complementary base pairs through hydrogen bonds.This structure ensures the stability of DNA and enables thec precise replication of genetic information[1].In biological processes,DNA collaborates with various biomolecules,including proteins,RNAs,small molecules,lipids,and carbohydrates[2].
