Dented n anospheres show promisi ng potential in drug delivery,nano motors,etc.However,it is still challe nging to prepare them by homopolymer self-assembly because of the strict structural requirements of the homopol...Dented n anospheres show promisi ng potential in drug delivery,nano motors,etc.However,it is still challe nging to prepare them by homopolymer self-assembly because of the strict structural requirements of the homopolymer.Herein,we propose a strategy for preparing dented nano spheres from homopolymers by co-assembly with a short peptide.They were co-assembled from poly(2-hydroxy-3-((4-(ethoxycarbonyl)phenyl)amino)propyl methacrylate)(PHBzoMA59)and(S)-2-((S)-2-((((9H-fluoren-9-yl)methoxy)carbonyl)amino)-3-phenylpro-panamido)-3-phenyIpropanoic acid(Fmoc-FF-OH).PHBzoMA homopolymers can only self-assemble into nanospheres without dent,and the addition of a short peptide in troduced hydroge n bondi ng and complementary π-π stacki ng interactions led to the final dented nano sphere morphology.The weight fracti ons of the short peptide can be adjusted to regulate the final morphology.It was con firmed that the radius of curvature of the dent on the surface was related to the organic bubble inside the protospheres prepared at critical aggregation concentration(CAC).The orga nic bubble can be adjusted by altering the kind of orga nic solve nt and solution pH,which allowed control over the dented nano sphere dimension.The use of differe nt orga nic solve nts with various polarities allows adjustment of the interfacial tension,and hence the denting degree.This degree can also be controlled by manipulating the solution pH to(de)protonate the short peptide and homopolymer.Furthermore,the versatility of this method was highlighted by using a differe nt homopolymer and the applicability of the resulting den ted nanospheres was demonstrated by decoration with gold nanoparticles.Overall,this study provided important insights and a new simple strategy to prepare den ted n anospheres in a con trolled fashion.展开更多
Dent disease is a rare X-linked recessively inherited renal tubulopathy,caused by variants in the CLCN5(Dent disease type 1,DD1)and OCRL(Dent disease type 2,DD2)genes,and characterized by low molecular weight proteinu...Dent disease is a rare X-linked recessively inherited renal tubulopathy,caused by variants in the CLCN5(Dent disease type 1,DD1)and OCRL(Dent disease type 2,DD2)genes,and characterized by low molecular weight proteinuria,hypercalciuria,microscopic hematuria,or nephrocalcinosis.In the current study,we collected and analyzed clinical data and genetic testing results of 21 children diagnosed with Dent disease,who were hospitalized at the Department of Nephrology,Children's Hospital of Nanjing Medical University between January 2014 and August 2023,aiming to assist in the early diagnosis and treatment of these patients.Among the 21 patients,16(76.19%)had CLCN5 variants,and five(23.81%)had OCRL variants,and four of the variants were novel.All patients presented with low molecular weight proteinuria,14(66.67%)of whom had nephroticrange proteinuria.Eight patients underwent renal biopsies because of diagnostic uncertainty.We transfected the novel CLCN5 missense variant(p.G222R)and OCRL missense variant(p.I371T)plasmids into both HEK293 and HK-2 cells and found a significantly lower expression of the OCRL1 protein in the transfected cells than in the wild-type cells(P<0.05).Moreover,we observed an extremely skewed X-chromosome inactivation pattern in a female patient carrying the same novel CLCN5 variant,as assessed by the human androgen receptor gene assay.These findings provide insight into the clinical characteristics of Dent disease in Chinese patients and may shed light on its pathogenesis.展开更多
Shaoxing Shuifu Reeds is professional on producing all kinds of Air Jet Reeds and Flat Reeds,which has an annual output of 90,000 meters air-jet reeds,300,000,000 pcs of profile dents and 60,000 meters of Flat Reeds,W...Shaoxing Shuifu Reeds is professional on producing all kinds of Air Jet Reeds and Flat Reeds,which has an annual output of 90,000 meters air-jet reeds,300,000,000 pcs of profile dents and 60,000 meters of Flat Reeds,Which has been honored with China Air Jet Profile Reeds Research Center by China Textile Machinery Association in 2015.In order to reach the demands of high quality fabric and industrial textile products,the yarn is developing to high count,multicolor,various fibers,and the fabric is developing to high count,high density,environment protection.So we have researched out high density,multicolor,multinozzle,double energy saving type profile reeds to let you choose.展开更多
Drop-weight impact tests were conducted on 2024-T3 aluminum plates with five types of impactors, and then the effects of the dent on the residual ultimate strength of the 2024-T3 specimens were investigated through ax...Drop-weight impact tests were conducted on 2024-T3 aluminum plates with five types of impactors, and then the effects of the dent on the residual ultimate strength of the 2024-T3 specimens were investigated through axial compression tests. Results indicate that with increase in dent depth, the five types of dents affect the ultimate strength of the plate in different trends. Nevertheless, other than the plate global deflection caused by impacting, the dent itself has unremarkable effect on the ultimate strength. The mathematical expressions are derived regarding the relationship between impact energy factor and the dent depth factor as well as the compressive ultimate strength reduction rate and the dent depth factor.展开更多
Based on ANSYS/LS-DYNA, the dynamics analysis software, and using nonlinear dynamic finite element method, the process of a submarine pipeline impacted by dropped objects is simulated, Some conclusions are drawn： Fir...Based on ANSYS/LS-DYNA, the dynamics analysis software, and using nonlinear dynamic finite element method, the process of a submarine pipeline impacted by dropped objects is simulated, Some conclusions are drawn： First, the damage depth of suspended pipe is smaller than that of the bare pipe in case of the same fall energy, and with the increase of fall energy, the difference grows; Second, with the falling object＇s speed and mass rising, the deformation of pipeline intensifies at the impact site and the maximum vibration amplitude of submarine pipeline increases; Third, when the fall energy is equal, the smaller the contact area of falling objects and pipeline is, the greater the damage depth of pipeline becomes; Fourth, changes of seabed soil parameters （shear elastic modulus, internal friction angle, density） have less influence on the suspended pipeline＇s dent depths and maximum vibration amplitude,展开更多
The shear failure of a rigid-plastic dented clamped tubular beam under the lateral impact of a mass is investigated. Both the denting and the impact point are in the middle span of the beam. It is assumed that denting...The shear failure of a rigid-plastic dented clamped tubular beam under the lateral impact of a mass is investigated. Both the denting and the impact point are in the middle span of the beam. It is assumed that denting does not spread during the shear sliding. Numerical results show that the axial force and lateral deflection of the beam are very small at the moment of the occurence of shear failure, which means that the finite deformation effect can be neglected in the shear failure analysis. Also, some aspects of the initial impact energy are investigated.展开更多
基金by the National Science Fund for Distinguished Young Scholars,NSFC(No.21925505)Z.F.is supported by the National Natural Science Foundation of China(Nos.51803152,22075212)+2 种基金the program for professor of special appointment(Eastern Scholar)at Shanghai institutions of higher learning and Natural Science Foundation of Shanghai(19ZR1478800)Y.Z.is supported by the National Natural Science Foundation of China(No.51903190)and Shanghai Pujiang Program(No.19PJ140960)EJ.C.is supported by the China Postdoctoral Science Foundation(No.2020M671197).
文摘Dented n anospheres show promisi ng potential in drug delivery,nano motors,etc.However,it is still challe nging to prepare them by homopolymer self-assembly because of the strict structural requirements of the homopolymer.Herein,we propose a strategy for preparing dented nano spheres from homopolymers by co-assembly with a short peptide.They were co-assembled from poly(2-hydroxy-3-((4-(ethoxycarbonyl)phenyl)amino)propyl methacrylate)(PHBzoMA59)and(S)-2-((S)-2-((((9H-fluoren-9-yl)methoxy)carbonyl)amino)-3-phenylpro-panamido)-3-phenyIpropanoic acid(Fmoc-FF-OH).PHBzoMA homopolymers can only self-assemble into nanospheres without dent,and the addition of a short peptide in troduced hydroge n bondi ng and complementary π-π stacki ng interactions led to the final dented nano sphere morphology.The weight fracti ons of the short peptide can be adjusted to regulate the final morphology.It was con firmed that the radius of curvature of the dent on the surface was related to the organic bubble inside the protospheres prepared at critical aggregation concentration(CAC).The orga nic bubble can be adjusted by altering the kind of orga nic solve nt and solution pH,which allowed control over the dented nano sphere dimension.The use of differe nt orga nic solve nts with various polarities allows adjustment of the interfacial tension,and hence the denting degree.This degree can also be controlled by manipulating the solution pH to(de)protonate the short peptide and homopolymer.Furthermore,the versatility of this method was highlighted by using a differe nt homopolymer and the applicability of the resulting den ted nanospheres was demonstrated by decoration with gold nanoparticles.Overall,this study provided important insights and a new simple strategy to prepare den ted n anospheres in a con trolled fashion.
基金supported by the National Natural Science Foundation of China(Grant No.82200744)the Nanjing Science and Technology Development Funding(Grant No.YKK22159).
文摘Dent disease is a rare X-linked recessively inherited renal tubulopathy,caused by variants in the CLCN5(Dent disease type 1,DD1)and OCRL(Dent disease type 2,DD2)genes,and characterized by low molecular weight proteinuria,hypercalciuria,microscopic hematuria,or nephrocalcinosis.In the current study,we collected and analyzed clinical data and genetic testing results of 21 children diagnosed with Dent disease,who were hospitalized at the Department of Nephrology,Children's Hospital of Nanjing Medical University between January 2014 and August 2023,aiming to assist in the early diagnosis and treatment of these patients.Among the 21 patients,16(76.19%)had CLCN5 variants,and five(23.81%)had OCRL variants,and four of the variants were novel.All patients presented with low molecular weight proteinuria,14(66.67%)of whom had nephroticrange proteinuria.Eight patients underwent renal biopsies because of diagnostic uncertainty.We transfected the novel CLCN5 missense variant(p.G222R)and OCRL missense variant(p.I371T)plasmids into both HEK293 and HK-2 cells and found a significantly lower expression of the OCRL1 protein in the transfected cells than in the wild-type cells(P<0.05).Moreover,we observed an extremely skewed X-chromosome inactivation pattern in a female patient carrying the same novel CLCN5 variant,as assessed by the human androgen receptor gene assay.These findings provide insight into the clinical characteristics of Dent disease in Chinese patients and may shed light on its pathogenesis.
文摘Shaoxing Shuifu Reeds is professional on producing all kinds of Air Jet Reeds and Flat Reeds,which has an annual output of 90,000 meters air-jet reeds,300,000,000 pcs of profile dents and 60,000 meters of Flat Reeds,Which has been honored with China Air Jet Profile Reeds Research Center by China Textile Machinery Association in 2015.In order to reach the demands of high quality fabric and industrial textile products,the yarn is developing to high count,multicolor,various fibers,and the fabric is developing to high count,high density,environment protection.So we have researched out high density,multicolor,multinozzle,double energy saving type profile reeds to let you choose.
基金Projects(13R21421700,13R21421800)sponsored by Shanghai Postdoctoral Scientific Program,China
文摘Drop-weight impact tests were conducted on 2024-T3 aluminum plates with five types of impactors, and then the effects of the dent on the residual ultimate strength of the 2024-T3 specimens were investigated through axial compression tests. Results indicate that with increase in dent depth, the five types of dents affect the ultimate strength of the plate in different trends. Nevertheless, other than the plate global deflection caused by impacting, the dent itself has unremarkable effect on the ultimate strength. The mathematical expressions are derived regarding the relationship between impact energy factor and the dent depth factor as well as the compressive ultimate strength reduction rate and the dent depth factor.
文摘Based on ANSYS/LS-DYNA, the dynamics analysis software, and using nonlinear dynamic finite element method, the process of a submarine pipeline impacted by dropped objects is simulated, Some conclusions are drawn： First, the damage depth of suspended pipe is smaller than that of the bare pipe in case of the same fall energy, and with the increase of fall energy, the difference grows; Second, with the falling object＇s speed and mass rising, the deformation of pipeline intensifies at the impact site and the maximum vibration amplitude of submarine pipeline increases; Third, when the fall energy is equal, the smaller the contact area of falling objects and pipeline is, the greater the damage depth of pipeline becomes; Fourth, changes of seabed soil parameters （shear elastic modulus, internal friction angle, density） have less influence on the suspended pipeline＇s dent depths and maximum vibration amplitude,
文摘The shear failure of a rigid-plastic dented clamped tubular beam under the lateral impact of a mass is investigated. Both the denting and the impact point are in the middle span of the beam. It is assumed that denting does not spread during the shear sliding. Numerical results show that the axial force and lateral deflection of the beam are very small at the moment of the occurence of shear failure, which means that the finite deformation effect can be neglected in the shear failure analysis. Also, some aspects of the initial impact energy are investigated.
