Endoplasmic reticulum(ER)homeostasis is vital to cell physiological functions.Glucose-regulated protein 78(GRP78)is a molecular chaperone considered a sensor of ER stress and participates in maintaining ER homeostasis...Endoplasmic reticulum(ER)homeostasis is vital to cell physiological functions.Glucose-regulated protein 78(GRP78)is a molecular chaperone considered a sensor of ER stress and participates in maintaining ER homeostasis.However,very few studies have provided detailed descriptions of crustacean GRP78 genes.The complete coding sequence of the GRP78 of Neocaridina denticulata sinensis was obtained and termed NdGRP78 in this study.Sequence analysis showed that the open reading frame of NdGRP78 encodes a protein of 656 amino acid residues,and the encoded protein includes three heat shock proteins 70(HSP70)family conservational motifs,one ATP/GTP binding site A,and one ER homolog region.Multiple sequence alignments demonstrated that the sequences of these key protein functional domains were highly conserved.Phylogenetic analysis revealed that the NdGRP78 protein belongs to a member of the HSP70 family.The NdGRP78 mRNA was detected in all tested tissues by real-time fluorescence quantitative PCR(qPCR),suggesting that the NdGRP78 mRNA was the constituent expression in N.denticulata sinensis.The in-situ hybridization result showed that the expression of NdGRP78 mRNA occurred in all types of hepatopancreas cells.Furthermore,the NdGRP78 expression was significantly increased when shrimp were transferred into Vibrio parahaemolyticus-EGFP infected conditions,low or high temperature,and hypoxia.The above results indicate that NdGRP78 expressions were associated with stress responses.This study provided a theoretical support to the good understanding of the role of NdGRP78 in response to different types of environmental stress tolerance.展开更多
Three new C-methylated and isoprenylated chalcone derivatives,dentichalcones A–C(1–3),together with six known compounds(4–9),were isolated from the twigs and leaves of Macaranga denticulata.Their structures were el...Three new C-methylated and isoprenylated chalcone derivatives,dentichalcones A–C(1–3),together with six known compounds(4–9),were isolated from the twigs and leaves of Macaranga denticulata.Their structures were elucidated by spectroscopic analysis,including 1D,2D NMR,and MS data.The known compounds,(2E)-1-(5,7-dihydroxy-2,2,6-trimethyl-2H-benzopyran-8-yl)-3-(4-methoxyphenyl)-2-propen-1-one(4),(2E)-1-(5,7-dihydroxy-2,2-dimethyl-2H-benzopyran-8-yl)-3-phenyl-2-propen-1-one(5),laxichalcone(6),macarangin(7),bonanniol A(8),and bonannione A(9),showed inhibitory activities against protein tyrosine phosphatase 1B(PTP1B)in vitro.Graphical Abstract Three new C-methylated and isoprenylated chalcone derivatives,dentichalcones A–C(1–3),together with six known compounds,were isolated from the twigs and leaves of Macaranga denticulata.Some compounds showed inhibitory activities against PTP1B in vitro.展开更多
The body surface of crustaceans is covered with a sturdy shell.The growth and development of crustaceans are realized through molting.Neocaridina denticulata sinensis is a suitable candidate for crustacean scientific ...The body surface of crustaceans is covered with a sturdy shell.The growth and development of crustaceans are realized through molting.Neocaridina denticulata sinensis is a suitable candidate for crustacean scientific research.Two calcium-associated cuticular protein genes,named NdCAP-1 and NdCAP-2,were obtained from N.denticulata sinensis.Molecular docking simulated the binding effect of both proteins and calcium ions.Semi-quantitative reverse transcription PCR results show that NdCAP-1 is expressed in D_(2-4) stage,NdCAP-2 expressed in D_(2-4) and A-B stages,and both were significantly expressed in the cephalothorax cuticle and pereiopods.Then,it was revealed that NdCAP-1 and NdCAP-2 are regulated by NdEcR-mediated 20 E signaling pathways.Knockdown of NdCAP-1 and NdCAP-2 was observed to cause surface defects.The recombinant proteins(rNdCAP-1 and rNdCAP-2),obtained by prokaryotic expression,had calcium-binding and chitin-binding ability,inhibited formation of calcium carbonate precipitate.These results show that calcium-associated cuticular proteins play important roles in cuticle formation and calcification.展开更多
基金Supported by the National Natural Science Foundation of China (Nos. 32172954, 32373121)the Key Research and Development Project of Hebei Province (No. 22323201D)+1 种基金the Science and Technology Project of Hebei Education Department (No. ZD2022093)the Natural Science Foundation of Hebei Province of China (No. D2022201003)
文摘Endoplasmic reticulum(ER)homeostasis is vital to cell physiological functions.Glucose-regulated protein 78(GRP78)is a molecular chaperone considered a sensor of ER stress and participates in maintaining ER homeostasis.However,very few studies have provided detailed descriptions of crustacean GRP78 genes.The complete coding sequence of the GRP78 of Neocaridina denticulata sinensis was obtained and termed NdGRP78 in this study.Sequence analysis showed that the open reading frame of NdGRP78 encodes a protein of 656 amino acid residues,and the encoded protein includes three heat shock proteins 70(HSP70)family conservational motifs,one ATP/GTP binding site A,and one ER homolog region.Multiple sequence alignments demonstrated that the sequences of these key protein functional domains were highly conserved.Phylogenetic analysis revealed that the NdGRP78 protein belongs to a member of the HSP70 family.The NdGRP78 mRNA was detected in all tested tissues by real-time fluorescence quantitative PCR(qPCR),suggesting that the NdGRP78 mRNA was the constituent expression in N.denticulata sinensis.The in-situ hybridization result showed that the expression of NdGRP78 mRNA occurred in all types of hepatopancreas cells.Furthermore,the NdGRP78 expression was significantly increased when shrimp were transferred into Vibrio parahaemolyticus-EGFP infected conditions,low or high temperature,and hypoxia.The above results indicate that NdGRP78 expressions were associated with stress responses.This study provided a theoretical support to the good understanding of the role of NdGRP78 in response to different types of environmental stress tolerance.
基金Financial support from the National Natural Science Foundation of China(Nos.81222045,21372049)the Specialized Research Fund for the Doctoral Program of Higher Education of China(20130071120104)the Shu Guang Project(No.12SG02)from Shanghai Municipal Education Commission and Shanghai Education Development Foundation is gratefully acknowledged.
文摘Three new C-methylated and isoprenylated chalcone derivatives,dentichalcones A–C(1–3),together with six known compounds(4–9),were isolated from the twigs and leaves of Macaranga denticulata.Their structures were elucidated by spectroscopic analysis,including 1D,2D NMR,and MS data.The known compounds,(2E)-1-(5,7-dihydroxy-2,2,6-trimethyl-2H-benzopyran-8-yl)-3-(4-methoxyphenyl)-2-propen-1-one(4),(2E)-1-(5,7-dihydroxy-2,2-dimethyl-2H-benzopyran-8-yl)-3-phenyl-2-propen-1-one(5),laxichalcone(6),macarangin(7),bonanniol A(8),and bonannione A(9),showed inhibitory activities against protein tyrosine phosphatase 1B(PTP1B)in vitro.Graphical Abstract Three new C-methylated and isoprenylated chalcone derivatives,dentichalcones A–C(1–3),together with six known compounds,were isolated from the twigs and leaves of Macaranga denticulata.Some compounds showed inhibitory activities against PTP1B in vitro.
基金Supported by the National Natural Science Foundation of China(Nos.32172954,32373121)the Key Research and Development Project of Hebei Province(No.22323201D)+2 种基金the Science and Technology Project of Hebei Education Department(No.ZD 2022093)the Natural Science Foundation of Hebei Province(Nos.D2022201003,D2023201002)the Hangzhou Qianjiang Special Expert fund for Prof.Jiquan ZHANG。
文摘The body surface of crustaceans is covered with a sturdy shell.The growth and development of crustaceans are realized through molting.Neocaridina denticulata sinensis is a suitable candidate for crustacean scientific research.Two calcium-associated cuticular protein genes,named NdCAP-1 and NdCAP-2,were obtained from N.denticulata sinensis.Molecular docking simulated the binding effect of both proteins and calcium ions.Semi-quantitative reverse transcription PCR results show that NdCAP-1 is expressed in D_(2-4) stage,NdCAP-2 expressed in D_(2-4) and A-B stages,and both were significantly expressed in the cephalothorax cuticle and pereiopods.Then,it was revealed that NdCAP-1 and NdCAP-2 are regulated by NdEcR-mediated 20 E signaling pathways.Knockdown of NdCAP-1 and NdCAP-2 was observed to cause surface defects.The recombinant proteins(rNdCAP-1 and rNdCAP-2),obtained by prokaryotic expression,had calcium-binding and chitin-binding ability,inhibited formation of calcium carbonate precipitate.These results show that calcium-associated cuticular proteins play important roles in cuticle formation and calcification.
