Automated parallel manipulation of multiple micro-objects with optoelectronic tweezers(OET)has brought significant research interests recently.However,the parallel manipulation of multiple objects in complex obstacle-...Automated parallel manipulation of multiple micro-objects with optoelectronic tweezers(OET)has brought significant research interests recently.However,the parallel manipulation of multiple objects in complex obstacle-dense microenvironment using OET technology based on negative dielectrophoresis(nDEP)remain a big technical challenge.In this work,we proposed an adaptive light pattern design strategy to achieve automated parallel OET manipulation of multiple micro-objects and navigate them through obstacles to target positions with high precision and no collision.We first developed a multi-micro-object parallel manipulation OET system,capable of simultaneous image processing and microparticles path planning.To overcome microparticle collisions caused by overlapping light patterns,we employed a novel adaptive light pattern design that can dynamically adjust the layout of overlapping light patterns according to surrounding environment,ensuring enough space for each microparticle and preventing unintended escapes from the OET trap.The efficacy of this approach has been verified through systematic simulations and experiments.Utilizing this strategy,multiple polystyrene microparticles were autonomously navigated through obstacles and microchannels to their intended destinations,demonstrating the strategy’s effectiveness and potential for automated parallel micromanipulation of multiple microparticles in complex and confined microenvironments.展开更多
基金the support from Optoseeker Biotechnology(Shenzhen)Co.,Ltd.to help build the OET system and provide Optobot 500 to carry out the experiment shown in supplementary Movie S4.National Key R&D Program of China(2023YFE0112400,2022YFA1207100,2024YFC3406900)National Natural Science Foundation of China(62103050,62473245,61933008)+6 种基金Beijing Municipal Natural Science Foundation(4242060,L246030)the Chongqing Municipal Natural Science Foundation(Grant No.2024NSCQJQX0192,CSTB2024NSCQ-JQX0034)the BIT Research and Innovation Promoting Project(Grant No.2023CX01002)Innovation Program of Shanghai Municipal Education Commission(2021-01-07-00-09-E0013)Shanghai Science and Technology plan project(23ZR1422300)Shenzhen Science and Technology Program(KJZD20240903101359020)Open Research Fund of Guangdong Provincial Key Laboratory of Advanced Biomaterials(Southern University of Science and Technology,Grant No.KLAB202404002).
文摘Automated parallel manipulation of multiple micro-objects with optoelectronic tweezers(OET)has brought significant research interests recently.However,the parallel manipulation of multiple objects in complex obstacle-dense microenvironment using OET technology based on negative dielectrophoresis(nDEP)remain a big technical challenge.In this work,we proposed an adaptive light pattern design strategy to achieve automated parallel OET manipulation of multiple micro-objects and navigate them through obstacles to target positions with high precision and no collision.We first developed a multi-micro-object parallel manipulation OET system,capable of simultaneous image processing and microparticles path planning.To overcome microparticle collisions caused by overlapping light patterns,we employed a novel adaptive light pattern design that can dynamically adjust the layout of overlapping light patterns according to surrounding environment,ensuring enough space for each microparticle and preventing unintended escapes from the OET trap.The efficacy of this approach has been verified through systematic simulations and experiments.Utilizing this strategy,multiple polystyrene microparticles were autonomously navigated through obstacles and microchannels to their intended destinations,demonstrating the strategy’s effectiveness and potential for automated parallel micromanipulation of multiple microparticles in complex and confined microenvironments.
