The purpose of this study was to investigate the hypoglycemic effect and mechanism of D endrobium officinale polysaccharide(DOP)on type 2 diabetes mellitus(T2DM)mice established by high-fat diet and streptozotocin.The...The purpose of this study was to investigate the hypoglycemic effect and mechanism of D endrobium officinale polysaccharide(DOP)on type 2 diabetes mellitus(T2DM)mice established by high-fat diet and streptozotocin.The results showed that DOP improved glycolipid metabolism and serum inflammation levels,and inhibited intestinal-derived lipopolysaccharide(LPS)translocation,suggesting that inhibiting LPS-mediated intestinal barrier damage may be a key target for DOP to alleviate T2DM.Interestingly,the study found that DOP reduced intestinal inflammation and oxidative stress levels,significantly up-regulated the mRNA expression of tight junction proteins Claudin-1,Occludin and zonula occluden-1(ZO-1),and ameliorated intestinal epithelial damage.In addition,DOP strongly inhibited the intestinal pathogenic bacteria and LPS-producing bacteria Helicobacter,Enterococcus and Desulfovibrio with a reduction rate of 95%,73% and 9%,respectively,and promoted the proliferation of anti-inflammatory bacteria Bifidobacterium and Lactobacillus by 139% and 8%,respectively.Taken together,the hypoglycemic effect of DOP was related to the protection of intestinal mucosal barrier,and its underlying mechanism lied in its excellent anti-inflammatory and gut microbiotamodulatory effects,providing a theoretical basis for developing DOP as a novel prebiotic in functional food for diabetes.展开更多
[ Objective ] The aim was to study the effects of polysaccharides of Dendrobium Huoshanense and Dendrobium moniliforme on oxygen free radicals and lipid peroxidation in vitro. [ Method] The scavenging action on .OH of...[ Objective ] The aim was to study the effects of polysaccharides of Dendrobium Huoshanense and Dendrobium moniliforme on oxygen free radicals and lipid peroxidation in vitro. [ Method] The scavenging action on .OH of polysaccharides, the inhibition action on O2^- of polysaccharides, MDA production of lipid peroxidation were measured by Fenton reaction, self oxidation system of C6H3（ OH）3 and TBA method respectively. [ Result] The half scavenging concentration on ·OH of potysaccharides of Dendrobium Huoshanense and Dendrobium moniliforme was 6.79 and 6.75 mg/ml respectively. As to their half inhibition concentration on O2 , the data was 3, 04 and 3.44 mg/ml respectively. Two kinds of Dendrobium polysaccharides bothhad inhibitory actions on lipid peroxidation of liver homogenate induced by auto-oxidation and inducedoxidation, and they could alleviate oxidation damages of mice liver mitochondria induced by Vc-Fe2＋ system. [Conclusion] Two kinds of Dendrobium polysacchaddes both had remarkable anti-oxidation activity in vitro.展开更多
[Objective]This study was to reveal the physiological and ecological mechanism of growth and development of test-tube seedlings.[Methods]Leaf stomata of test-tube seedlings of D.loddigesii and D.candidum at different ...[Objective]This study was to reveal the physiological and ecological mechanism of growth and development of test-tube seedlings.[Methods]Leaf stomata of test-tube seedlings of D.loddigesii and D.candidum at different growth and development stages were observed under scanning electron microscope,and their fluorescence induction response curve was assayed by using modulate fluorometer.[Results] At each growth and development stage,D.loddigesii test-tube seedling has a higher leaf stomatal density over D.candidum,while a lower stomata opening rate.Along with the growth,the size of stomata of both D.loddigesii and D.candidum did not change obviously,but their stoma density increased by 83% and 17.6% respectively.Leaf stomata opening rate reached the highest at the age of 240-day-old.Under the conditions of three settled light intensities,stomatal opening degree of D.loddigesii was increased by the intensity of illumination,the maximum aperture occurred at the light intensity of 54 μmol/m2·s.Given the same culture condition,both the maximum electron transport rate（14 μmol/m2·s）and light saturation point（318 μmol/m2·s）of D.loddigesii test-tube seedling are lower than that of D.candidum（20 μmol/m2·s,483 μmol/m2·s）.Moreover,the potential photosynthetic capacity of D.loddigesii is relatively poor.[Conclusion]The photosynthetic rate varies among different species of Dendrobium,which could be attributed to their different stomatal characteristics.Usually,the light intensity of tissue culture chamber is set up as 27 μmol/（m2·s）,where both D.loddigesii and D.candidum do not acquire an optimal state for their leaf stomatal opening.Considering the characteristics to select suitable culture condition,the stomatal aperture and photosynthetic rate could be enhanced,which can promote the growth and development of test-tube seedlings.展开更多
Dendrobium officinale is an orchid herb distinguished by its exceptional drought resistance capabilities.The remarkable drought tolerance of D.officinale stems from its structural and compositional features,including ...Dendrobium officinale is an orchid herb distinguished by its exceptional drought resistance capabilities.The remarkable drought tolerance of D.officinale stems from its structural and compositional features,including thick leaves and stems containing abundant polysaccharides and colloidal substances.Despite these adaptations,the underlying molecular mechanisms responsible for enhanced drought tolerance remain inadequately understood.This study subjected D.officinale to water restriction for periods ranging from 1 to 6 months,conducting physiological and RNA sequencing analyses to elucidate its long-term dehydration response mechanisms and identify drought-protective genes.Following 6 months of dehydration,D.officinale maintained viability,demonstrated by rapid growth resumption after merely 2 d of rehydration.Transcriptome analysis of D.officinale plants under 1-month dehydration revealed differential gene expression across various processes,predominantly in stress responses,photosynthesis,phytohormone signaling,carbon metabolism,and fructose/mannose pathways.Among these,PEROXIDASE4(POD4)and NAC37 showed significant upregulation and were selected for further investigation of their roles in drought protection.Transgenic tomato plants overexpressing D.officinale’s POD4 and NAC37 genes exhibited superior drought tolerance compared to controls,displaying enhanced vigor,increased fruit production,higher respiration rates,elevated chlorophyll levels,and reduced oxidative damage.This research demonstrates the value of exploring underutilized species for drought-tolerance genes and identifies POD4 and NAC37 as promising candidates for improving drought tolerance through breeding programs.展开更多
Heavy metal pollution has become a pervasive environmental issue affecting numerous regions worldwide.Recently,there has been significant attention given to the application of nano-enabled technologies with the purpos...Heavy metal pollution has become a pervasive environmental issue affecting numerous regions worldwide.Recently,there has been significant attention given to the application of nano-enabled technologies with the purpose of enhancing plant development and alleviating heavy metal stress.This study aimed to illustrate the potential of zinc oxide nanoparticles(ZnO-NPs)to enhance the morphological traits of D.huoshenense exposed to cadmium(Cd)stress.The chemical structure and elemental composition of the ZnO-NPs were characterised by a series of analytical methods,including X-ray diffraction,UV-Vis spectrometry,XPS,andTEM.Plant samples usedwere collected at 0,5,and 15 days in order to assess physiological and biochemical parameters under different Cd treatments.ZnONPs administered in pot experiments have been shown to enhance plant proliferation through the modulation of Cd enrichment levels.The results revealed that ZnO-NPs enhanced plant growth by increasing soluble sugars and proline levels,enhancing activities of antioxidant enzymes(SOD,POD,CAT,APX)and reducing electrolyte leakage(EL)and malondialdehyde(MDA)content.Furthermore,ZnO-NPs enhanced the net photosynthetic rate,transpiration,stomatal conductance,and chlorophyll content in leaves subjected to Cd stress at the 10-day sampling stage.Exogenous ZnO-NPs significantly elevated the expression of genes associated with flavonoid biosynthesis,potentially facilitating the accumulation of medicinal compounds to mitigate Cd stress.Taken together,these findings provide a novel perspective on the strategies employed by medicinal plants in response to Cd.展开更多
Dendrobium officinale(DO)is a well-recognized medicinal and edible plant with a long history of application in traditional medicinal practices across China and Southeast Asia.Recent studies have demonstrated that DOis...Dendrobium officinale(DO)is a well-recognized medicinal and edible plant with a long history of application in traditional medicinal practices across China and Southeast Asia.Recent studies have demonstrated that DOis abundant in diverse bioactive compounds,including polysaccharides(DOP),flavonoids,alkaloids,and bibenzyls thought to exert a range of pharmacological effects,such as anti-tumor and immunomodulatory effects.However,our comprehensive understanding of two key aspects-pharmacological functions and biosynthetic mechanisms-of DO’s major constituents remains limited,especiallywhen considered within the clinical contexts of traditional use.To address this gap,this study reviews DO’s historical applications,clinical effects,and related formulations through an analysis of ancient texts spanning nearly two millennia-with special attention to region-specific traditional medical texts.This provides a historical and empirical foundation for further exploration of its modern pharmacological potential.Given the central role of DOP in DO’s biological activities,this paper further summarizes its therapeutic applications across various diseases and the underlying mechanisms,with special emphasis on structure-activity relationships.This focus is particularly important because the structural characteristics of DOP are highly dependent on extraction and analytical methods,which have contributed to inconsistencies in pharmacological findings over the past two decades.Finally,the review highlights recent advances in the understanding of the in vivo biosynthesis and regulatory mechanisms of themajor bioactive components inDO,with a particular focus onmolecular regulation and responses to agricultural interventions.These factors are critical for the production of high-quality DO.Overall,this study develops a comprehensive knowledge framework that connects DO’s traditional applications of DO to its two key research areas:pharmacological functions and quality formation.We anticipate this framework will offer clear guidance for future research from a clinical perspective.展开更多
Background:Skin photoaging is a physiological or pathological process caused by multiple factors.Developing anti-skin photoaging drugs is a hot topic in cosmetology research fields.The purpose of this study was to exp...Background:Skin photoaging is a physiological or pathological process caused by multiple factors.Developing anti-skin photoaging drugs is a hot topic in cosmetology research fields.The purpose of this study was to explore the therapeutic effect of Dendrobium officinale(D.officinale)on skin aging.Methods:The ingredients of D.officinale were detected by UHPLC-Q-TOF/MS.The targets of D.officinale were screened by Swiss Target Prediction database.GeneCards,NCBI,and OMIM databases were utilized to find out the targets associated with skin photoaging.Overlapping targets of D.officinale and skin photoaging were obtained by Venn analysis.The ingredient-disease target network and protein-protein interaction network were constructed by using the STRING database and Cytoscape software.The key compounds and hub genes were obtained by analyzing networks.The DAVID database was applied for Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analysis of the overlapping targets.Autodock Vina software was used to simulate molecular docking and the results were visualized using Pymol.Finally,the skin photoaging models of cells and mice were established to validate the results predicted by network pharmacology.Results:In D.officinale,a total of 59 compounds and 595 targets were detected,of which 59 proteins were intersectional with skin photoaging targets.The top 10 active ingredients(Dendrophenol,Herbacetin,Lyoniresinol,Trans-ferulaldehyde,Naringenin,and so on)and 8 hub genes(AKT1,TNF,VEGFA,MAPK3,CASP3,MMP9,CTNNB1,and EGFR)were identified.All the key active compounds could bind well with core protein targets(binding energy<-5 kcal/mol).The potential therapeutic targets were related to the response to reactive oxygen species,collagen catabolic process,extracellular matrix organization,and apoptotic process,mainly.We also found that D.officinale could enhance the cell viability and activity of anti-oxidases,reduce reactive oxygen species and MMP9 levels,and stable mitochondrial membrane potential.Furthermore,D.officinale could alleviate skin photoaging injury and reduce malondialdehyde level in mice.Conclusion:D.officinale alleviated skin photoaging via regulating oxidative stress,apoptosis,and collagen catabolic process.展开更多
The aim of this study was to investigate the role of Dendrobium officinale crude polysaccharides(DOPS)and D.officinale purified polysaccharides(DOPS100)in attenuating immune dysfunction in subfertile mice.The results ...The aim of this study was to investigate the role of Dendrobium officinale crude polysaccharides(DOPS)and D.officinale purified polysaccharides(DOPS100)in attenuating immune dysfunction in subfertile mice.The results of the study revealed several noteworthy findings.First,DOPS and DOPS100 treatments led to significant improvement in weight gain and reversal of fatigue-related behaviors compared to the normal group.Second,both DOPS and DOPS100 showed effectiveness in reducing immune organ swelling,modulating immunoglobulin A(IgA)and immunoglobulin M(IgM)levels,and restoring complement(C3c and C4)levels.In addition,they demonstrated a significant ability to enhance the integrity of the intestinal mechanical barrier by differentially upregulating the tight junction proteins Occludin and Zonula occludens 1(ZO-1).In addition,it was found that DOPS100 specifically enhanced the CD4^(+)-T helper 17 cell(Th17)/regulatory T cell(Treg)immune axis in the gut,as evidenced by increased expression of forkhead box protein 3(Foxp3)as well as decreased expression of retinoic acid receptor-related orphan receptor γt(RORγt),and further modulation of interleukin 10(IL-10),interleukin 22(IL-22),and interleukin 17A(IL-17A)expression levels of inflammatory factors.These findings collectively suggest that DOPS100 holds significant potential in improving sub-healthy status by repairing the intestinal barrier,restoring local immune homeostasis,and activating the intestinal immune regulatory network.The study’s outcomes provide valuable insights into the therapeutic implications of DOPS and DOPS100 in addressing immune dysfunction in sub-healthy conditions.展开更多
为比较春石斛栽培品种Dendrobium Second Love‘Tokimeki’与金钗石斛的粗多糖、精多糖(除蛋白多糖)和多糖水洗组分(过层析柱分离的水洗组分)的免疫活性差异,分别对昆明小鼠灌胃上述6种不同纯度的多糖,观察其对小鼠脏体指数、迟发性变...为比较春石斛栽培品种Dendrobium Second Love‘Tokimeki’与金钗石斛的粗多糖、精多糖(除蛋白多糖)和多糖水洗组分(过层析柱分离的水洗组分)的免疫活性差异,分别对昆明小鼠灌胃上述6种不同纯度的多糖,观察其对小鼠脏体指数、迟发性变态反应、抗体积数和小鼠腹腔巨噬细胞吞噬百分比与吞噬指数等免疫学指标的影响。结果表明:2种石斛的免疫活性随多糖纯度的提高而增强,纯度最高的多糖水洗组分免疫活性最强,且各指标均显著高于对照。D.Second Love‘Tokimeki’和金钗石斛同等纯度的多糖在胸腺指数、脾脏指数、小鼠耳肿胀率及小鼠抗体积数上均无显著差异,而D.Second Love‘Tokimeki’的3种不同纯度多糖的吞噬指数均达到了与金钗石斛多糖水洗组分无显著差异的水平。综合来看,栽培品种D.Second Love‘Tokimeki’的多糖免疫活性与金钗石斛多糖相近,具有良好的药用开发价值。展开更多
To study the antioxidant constituents from the stems of Dendrobium nobile, and to discuss theft structure-activity relationship. Compounds were isolated from a 60% ethanolic extract by various chromatographic techniqu...To study the antioxidant constituents from the stems of Dendrobium nobile, and to discuss theft structure-activity relationship. Compounds were isolated from a 60% ethanolic extract by various chromatographic techniques and were identified by spectral analysis. The antioxidant activities of compounds were evaluated by DPPH free radical scavenging assay. Five phenanthrenes and four lignans were obtained from the active fractions ofD. nobile. Their structures were identified as fimbriatone （1）, confusarin （2）, flavanthrinin （3）, 2,5-dihydroxy-4,9-dimethoxyphenanthrene （4）, 3,7-dihydroxy-2,4-dimethoxyphenanthrene （5）, syringaresinol （6）, pinoresinol （7）, medioresinol （8） and lirioresinol-A （9）, respectively. Compounds 2 and 6 exhibited more potent DPPH scavenging activities than vitamin C. All the above compounds were reported from this plant for the first time, and compounds 3, 4 and 9 were reported for the first time from the genus of Dendrobiurn. For all phenanthrenes and lignans, an electron-donating methoxyl group in the ortho position to the phenolic hydroxyl group exhibits enhanced antioxidant activities.展开更多
[Objective] This study aimed to explore the inoculation effects of Dendrobium officinale mycorrhizal fungi on their plantlets. [Method] Endophytic strains Tj1, Tj2 and Tj3 were obtained by isolation and purification f...[Objective] This study aimed to explore the inoculation effects of Dendrobium officinale mycorrhizal fungi on their plantlets. [Method] Endophytic strains Tj1, Tj2 and Tj3 were obtained by isolation and purification from mycorrhiza of wild Dendrobium officinale and inoculated on the root system of Dendrobium officinale for inoculation test. [Result] Under tissue-culture conditions, at early stage, Tj1 strain hadn＇t shown promotion effect on Dendrobium officinale, Tj2 strain had shown relatively strong promotion effects, and Tj3 strain had promoted the growth of roots; at late stage, Tj1 strain had shown relatively strong promotion effects, Tj2 strain had shown the best inoculation effects and the strongest promotion effects, while Tj3 strain had caused root and seedling rot problems of the plantlets; under outdoor conditions, after inoculation with Tj2 strain, the number of leaves and lateral buds were increased, the growth of lateral root and the increase of plant height were significant, the leaves of Dendrobium officinale plantlets were large and dark green and an obvious root enlargement phenomenon was observed. [Conclusion] The two inoculation methods both indicate that Tj2 strain has relatively strong promotion effects on the growth of Dendrobium officinale roots and shoots, the increase of plant number and plant height, and the germination of new shoots and roots, which proved the effective establishment of symbiotic relationship between Tj2 strain and Dendrobium officinale. Therefore, T2 strain has practical application values on the successful cultivation of Dendrobium officinale plantlets.展开更多
[Objective]The aim was to select the suitable hormone factors for flowering induction in vitro culture of Dendrobium officinate Kimura et Migo.[Method]The test-tube plantlets from the stems of Dendrobium officinate Ki...[Objective]The aim was to select the suitable hormone factors for flowering induction in vitro culture of Dendrobium officinate Kimura et Migo.[Method]The test-tube plantlets from the stems of Dendrobium officinate Kimura et Migo were used as the experimental materials and MS medium as the basic medium.Comparative tests have been done between single-factor hormone treatments（different concentrations of PP333 or TDZ） and multi-factor hormone treatments（different combinations of PP333,TDZ,6-BA and NAA） to research the effects of hormone factors on the flowering induction of the plantlets.[Result]Among the single-factor hormone treatments,the suitable concentration and the rate of flower buds formation of PP333 treatment were 0.2 mg/L and 8.5%,the that of TDZ treatment were 0.06 mg/L and 15.5%;the effects of multi-factor hormone treatments on the flowering induction were ordered as follow：（PP333 ＋ 6-BA ＋ NAA ＋ TDZ）〉 （PP333 ＋ 6-BA ＋ NAA）〉 （PP333 ＋ 6-BA） and（PP333 ＋ NAA） ;the most suitable treatment was PP333 0.3 mg/L ＋ 6-BA 0.5 mg/L ＋ NAA 0.5 mg/L ＋ TDZ 0.06 mg/L,the rate of flower bud formation and the rate of the blossomed flower were respectly reached to 80.4% and 90.3%.[Conclusion]PP333 and TDZ showed the important effect on the flowering induction in vitro culture of Dendrobium officinate Kimura et Migo.The effect of TDZ was better than that of PP333.It is much more conducive to the flower bud formation,when using appropriate concentration of TDZ combined with other hormones properly.展开更多
[Objective] This study aimed to analyse the genetic diversity of and genet- ic relationship between 33 Dendrobium species by using RSAP （Restriction Site Amplification Polymorphism） marker technique. [Method] A PCR-...[Objective] This study aimed to analyse the genetic diversity of and genet- ic relationship between 33 Dendrobium species by using RSAP （Restriction Site Amplification Polymorphism） marker technique. [Method] A PCR-reaction system was established, by which 33 pairs of primers screened from 45 pairs were used to con- duct the PCR amplification. [Results] The 33 pairs of primers produced 2 047 ampli- fied fragments, of which 2 044 were polymorphic, with a polymorphism rate of 99.8%, and each pair of primers produced 61.9 polymorphic fragments on average. The genetic similarity coefficients between species ranged from 0.081 to 0.442, sug- gesting that the experimental materials used in this study possess a wide genetic background. According to the result of RSAP, the 33 Dendrobium species were di- vided into 6 groups by the cluster analysis （UPGMA）. The result showed that the genetic relationship between D. acinaciforme and D. guangxiense was the closest, and that between D. sinense and either D. lituiflorum or D. hancockii was the fur- thest. [Conclusion] The result suggested that Dendrobium germplasms have a great genetic diversity. This study provides a molecular basis for the classification and breeding of Dendrobium plants, as well as the selection of hybridization parents in the future.展开更多
[Objective] This research aimed to establish an inductively coupled plasma mass spectrometry （ICP-MS） method for determination of five kinds of heavy metals （Pb, Cd, Cu, As and Hg） in Dendrobium candidium Wall. ex...[Objective] This research aimed to establish an inductively coupled plasma mass spectrometry （ICP-MS） method for determination of five kinds of heavy metals （Pb, Cd, Cu, As and Hg） in Dendrobium candidium Wall. ex Lindl. [Method] The samples were digested in HNO3-H202 acids system by closed vessel microwave di- gestion. At the same time, internal standard was added to avoid the matrix effect. [Result] The five kinds of heavy meals all showed good linear relationships, and the correlation coefficients ranged from 0.998 2 to 0.999 9. The detection limits of the five kinds of heavy metals were in the range of 0.008-0.750 μg/L, while the stan- dard recovery rates were in the range of 90.4%-96.9%. The relative standard devi- ations ranged from 1.6% to 8.2%. The heavy metals in the Ginseng and Astragalus national standard samples were determined by the established ICP-MS method, and the results were in conformity with the standard values. [Conclusion] The established method has simple and convenient operation and accurate and reliable results, and it meets the requirements by determination of heavy metals in Dendrobium candididium Wall. ex Lindl.展开更多
[Objective] To optimize the techniques for culturing Cr-enriched Dendrobi-um candidum protocorm. [Method] By adopting Plackett-Burman test, the effects of time, temperature, Cr-content in medium, NAA content, KT conte...[Objective] To optimize the techniques for culturing Cr-enriched Dendrobi-um candidum protocorm. [Method] By adopting Plackett-Burman test, the effects of time, temperature, Cr-content in medium, NAA content, KT content and light intensi-ty on Cr-enriched protocorm were researched. Three factors of time, Cr-content in medium and light intensity had statistical y significant effects; then steepest ascent procedures were applied to define optimal response region of these three factors; fi-nal y the optimal factors were determined by RSM analysis. [Result] The results showed that the optimal conditions for Cr-enrichment were chromium concentration at 0.37 mg/L, culture time of 59 d and il umination intensity of 1 822.22 lx. The predicted value of Cr-content in protocorm was 5.08 mg/kg. [Conclusion] RSM can optimize the techniques for culturing Cr-enriched protocorm of Dendrobium can-didum, provide the optimum process parameters and lay the foundation for further research.展开更多
In summer, Cha No. 39 of 292 bags and 205 of 244 bags were inter- planted with Dendrobium officinale on cultivation bed, respectively, whose yields and biological efficiencies reached 102.46 kg and 103.98%, and 96.77 ...In summer, Cha No. 39 of 292 bags and 205 of 244 bags were inter- planted with Dendrobium officinale on cultivation bed, respectively, whose yields and biological efficiencies reached 102.46 kg and 103.98%, and 96.77 kg and 113.32%, accordingly. The seedlings of Dendrobium officinale interplanted with Pleurotus os- treatus grew by 2.78 cm during 10 weeks on cultivation beds, and the seedlings grew by 2.68 cm in the cultivation beds without interplanting arrangement. These demonstrated that the interplanting of Dendrobium officinale and Pleurotus ostreatus has none adverse effects on growth of Dendrobium officinale.展开更多
To optimize the technique of rapid propagation of Dendrobium hybrida seedlings and to explore a hormone-free tissue culture method for D.hybrida,six kinds of mycorrhizal fungi which were isolated from the wild orchids...To optimize the technique of rapid propagation of Dendrobium hybrida seedlings and to explore a hormone-free tissue culture method for D.hybrida,six kinds of mycorrhizal fungi which were isolated from the wild orchids were made into fungal elicitors.These fungal elicitors were added into the DE medium with concentrations of 40,60 and 80 ml/L,respectively.After a 90-d culturing,the effects of fungal elicitors on the growth of D.hybrida cultivar ‘088＇ tissue culture seedlings were studied.The results showed that treatment 13（T13） extremely significantly increased the fresh weight,but other treatment groups had no significant effects.In addition,T1,T5,T9,T11 and T13 extremely significantly influenced the contents of chlorophyll a and chlorophyll a ＋ b.However,T1 and T11 had extremely significantly effect on the content of chlorophyll b.Combining the effects on fresh weight and chlorophyll content,it could be concluded T13（40 ml/L of Y05） has promoting effects on the growth of D.hybrida tissue culture seedlings.展开更多
T o investigate cold tolerance of different cultivars and species in Dendrobium Sw. in North China, 8 strains of Dendrobium officinal and other 5 Dendrobium species were used to compare their cold-hardiness index in t...T o investigate cold tolerance of different cultivars and species in Dendrobium Sw. in North China, 8 strains of Dendrobium officinal and other 5 Dendrobium species were used to compare their cold-hardiness index in this study. The content of electrolyte leakage was increasing in Dendrobium Sw. as the temperature decreasing, while the content of peroxidase(POD) activity and the soluble sugar showed a rising trend before a downward trend. Besides, external morphology was another evaluation standard of cold tolerance. Thus, there was a significant difference between Dendrobium officinal and other Dendrobium species in the cold tolerance, and their cold tolerance capacity was as follows: s1 > s100 >s13 > 1301 > s300 > Dendrobium nobile > s126 > s145 > s95 > Dendrobium lindleyi > Dendrobium chrysotoxum > Dendrobium thyrsiflorum > Dendrobium lohohense.展开更多
A new fluorenone derivative, named chrysotoxone(3) was isolated from the ethanolic extract of the stems of Dendrobium chrysotoxum (Orchidaceae), along with three known compounds. The structure of the new fluorenone wa...A new fluorenone derivative, named chrysotoxone(3) was isolated from the ethanolic extract of the stems of Dendrobium chrysotoxum (Orchidaceae), along with three known compounds. The structure of the new fluorenone was elucidated as 2,5,8trihydroxy1,4dimethoxy9fluorenone.展开更多
基金The Guangdong Basic and Applied Basic Research Foundation(2022A1515010730)the National Natural Science Foundation of China(32001647)+2 种基金the Science and Technology Plan Projects of Guangzhou City(202102100009)(the Guangzhou Key Laboratory for Intelligent Sensing and Quality Control of Agricultural Products)National Natural Science Foundation of China(31972022)the 111 Project(B17018)to conduct the project are gratefully acknowledged。
文摘The purpose of this study was to investigate the hypoglycemic effect and mechanism of D endrobium officinale polysaccharide(DOP)on type 2 diabetes mellitus(T2DM)mice established by high-fat diet and streptozotocin.The results showed that DOP improved glycolipid metabolism and serum inflammation levels,and inhibited intestinal-derived lipopolysaccharide(LPS)translocation,suggesting that inhibiting LPS-mediated intestinal barrier damage may be a key target for DOP to alleviate T2DM.Interestingly,the study found that DOP reduced intestinal inflammation and oxidative stress levels,significantly up-regulated the mRNA expression of tight junction proteins Claudin-1,Occludin and zonula occluden-1(ZO-1),and ameliorated intestinal epithelial damage.In addition,DOP strongly inhibited the intestinal pathogenic bacteria and LPS-producing bacteria Helicobacter,Enterococcus and Desulfovibrio with a reduction rate of 95%,73% and 9%,respectively,and promoted the proliferation of anti-inflammatory bacteria Bifidobacterium and Lactobacillus by 139% and 8%,respectively.Taken together,the hypoglycemic effect of DOP was related to the protection of intestinal mucosal barrier,and its underlying mechanism lied in its excellent anti-inflammatory and gut microbiotamodulatory effects,providing a theoretical basis for developing DOP as a novel prebiotic in functional food for diabetes.
文摘[ Objective ] The aim was to study the effects of polysaccharides of Dendrobium Huoshanense and Dendrobium moniliforme on oxygen free radicals and lipid peroxidation in vitro. [ Method] The scavenging action on .OH of polysaccharides, the inhibition action on O2^- of polysaccharides, MDA production of lipid peroxidation were measured by Fenton reaction, self oxidation system of C6H3（ OH）3 and TBA method respectively. [ Result] The half scavenging concentration on ·OH of potysaccharides of Dendrobium Huoshanense and Dendrobium moniliforme was 6.79 and 6.75 mg/ml respectively. As to their half inhibition concentration on O2 , the data was 3, 04 and 3.44 mg/ml respectively. Two kinds of Dendrobium polysaccharides bothhad inhibitory actions on lipid peroxidation of liver homogenate induced by auto-oxidation and inducedoxidation, and they could alleviate oxidation damages of mice liver mitochondria induced by Vc-Fe2＋ system. [Conclusion] Two kinds of Dendrobium polysacchaddes both had remarkable anti-oxidation activity in vitro.
基金Supported by the Key Projects in the National Science & Technology Pillar Program during 11th 5-year Plan Period(2006BAI06A11-11)~~
文摘[Objective]This study was to reveal the physiological and ecological mechanism of growth and development of test-tube seedlings.[Methods]Leaf stomata of test-tube seedlings of D.loddigesii and D.candidum at different growth and development stages were observed under scanning electron microscope,and their fluorescence induction response curve was assayed by using modulate fluorometer.[Results] At each growth and development stage,D.loddigesii test-tube seedling has a higher leaf stomatal density over D.candidum,while a lower stomata opening rate.Along with the growth,the size of stomata of both D.loddigesii and D.candidum did not change obviously,but their stoma density increased by 83% and 17.6% respectively.Leaf stomata opening rate reached the highest at the age of 240-day-old.Under the conditions of three settled light intensities,stomatal opening degree of D.loddigesii was increased by the intensity of illumination,the maximum aperture occurred at the light intensity of 54 μmol/m2·s.Given the same culture condition,both the maximum electron transport rate（14 μmol/m2·s）and light saturation point（318 μmol/m2·s）of D.loddigesii test-tube seedling are lower than that of D.candidum（20 μmol/m2·s,483 μmol/m2·s）.Moreover,the potential photosynthetic capacity of D.loddigesii is relatively poor.[Conclusion]The photosynthetic rate varies among different species of Dendrobium,which could be attributed to their different stomatal characteristics.Usually,the light intensity of tissue culture chamber is set up as 27 μmol/（m2·s）,where both D.loddigesii and D.candidum do not acquire an optimal state for their leaf stomatal opening.Considering the characteristics to select suitable culture condition,the stomatal aperture and photosynthetic rate could be enhanced,which can promote the growth and development of test-tube seedlings.
基金supported by the Hubei Provincial Natural Science Foundation,China(2022CFB009)the National Natural Science Foundation of China(31900242).
文摘Dendrobium officinale is an orchid herb distinguished by its exceptional drought resistance capabilities.The remarkable drought tolerance of D.officinale stems from its structural and compositional features,including thick leaves and stems containing abundant polysaccharides and colloidal substances.Despite these adaptations,the underlying molecular mechanisms responsible for enhanced drought tolerance remain inadequately understood.This study subjected D.officinale to water restriction for periods ranging from 1 to 6 months,conducting physiological and RNA sequencing analyses to elucidate its long-term dehydration response mechanisms and identify drought-protective genes.Following 6 months of dehydration,D.officinale maintained viability,demonstrated by rapid growth resumption after merely 2 d of rehydration.Transcriptome analysis of D.officinale plants under 1-month dehydration revealed differential gene expression across various processes,predominantly in stress responses,photosynthesis,phytohormone signaling,carbon metabolism,and fructose/mannose pathways.Among these,PEROXIDASE4(POD4)and NAC37 showed significant upregulation and were selected for further investigation of their roles in drought protection.Transgenic tomato plants overexpressing D.officinale’s POD4 and NAC37 genes exhibited superior drought tolerance compared to controls,displaying enhanced vigor,increased fruit production,higher respiration rates,elevated chlorophyll levels,and reduced oxidative damage.This research demonstrates the value of exploring underutilized species for drought-tolerance genes and identifies POD4 and NAC37 as promising candidates for improving drought tolerance through breeding programs.
基金supported by the Open Fund of Anhui Engineering Research Center for Ecoagriculture of Traditional Chinese Medicine(WXZR202318)High-level Talents Research Initiation Fund of West Anhui University(WGKQ2022025)+3 种基金Quality Engineering Project of West Anhui University(wxxy2024011)Quality Engineering Project of Anhui Province(2024zybj032)Development of Big Data Integration and Analysis Platform for Traditional Chinese Medicine Genomics(0045025050)Anhui Innovation and Entrepreneurship Training Program for College Students(S202510376030).
文摘Heavy metal pollution has become a pervasive environmental issue affecting numerous regions worldwide.Recently,there has been significant attention given to the application of nano-enabled technologies with the purpose of enhancing plant development and alleviating heavy metal stress.This study aimed to illustrate the potential of zinc oxide nanoparticles(ZnO-NPs)to enhance the morphological traits of D.huoshenense exposed to cadmium(Cd)stress.The chemical structure and elemental composition of the ZnO-NPs were characterised by a series of analytical methods,including X-ray diffraction,UV-Vis spectrometry,XPS,andTEM.Plant samples usedwere collected at 0,5,and 15 days in order to assess physiological and biochemical parameters under different Cd treatments.ZnONPs administered in pot experiments have been shown to enhance plant proliferation through the modulation of Cd enrichment levels.The results revealed that ZnO-NPs enhanced plant growth by increasing soluble sugars and proline levels,enhancing activities of antioxidant enzymes(SOD,POD,CAT,APX)and reducing electrolyte leakage(EL)and malondialdehyde(MDA)content.Furthermore,ZnO-NPs enhanced the net photosynthetic rate,transpiration,stomatal conductance,and chlorophyll content in leaves subjected to Cd stress at the 10-day sampling stage.Exogenous ZnO-NPs significantly elevated the expression of genes associated with flavonoid biosynthesis,potentially facilitating the accumulation of medicinal compounds to mitigate Cd stress.Taken together,these findings provide a novel perspective on the strategies employed by medicinal plants in response to Cd.
基金funded by the Scientific and technological innovation project of China Academy of Chinese Medical Sciences(CI2023E002,CI2024E003)National Natural Science Foundation of China(82304663)+1 种基金the Fundamental Research Funds for the Central Public Welfare Research Institutes(ZZ16-XRZ-072,ZZ17-YQ-025,ZXKT22052,and ZXKT22060)the National Key Research and Development Program(2022YFC3501803).
文摘Dendrobium officinale(DO)is a well-recognized medicinal and edible plant with a long history of application in traditional medicinal practices across China and Southeast Asia.Recent studies have demonstrated that DOis abundant in diverse bioactive compounds,including polysaccharides(DOP),flavonoids,alkaloids,and bibenzyls thought to exert a range of pharmacological effects,such as anti-tumor and immunomodulatory effects.However,our comprehensive understanding of two key aspects-pharmacological functions and biosynthetic mechanisms-of DO’s major constituents remains limited,especiallywhen considered within the clinical contexts of traditional use.To address this gap,this study reviews DO’s historical applications,clinical effects,and related formulations through an analysis of ancient texts spanning nearly two millennia-with special attention to region-specific traditional medical texts.This provides a historical and empirical foundation for further exploration of its modern pharmacological potential.Given the central role of DOP in DO’s biological activities,this paper further summarizes its therapeutic applications across various diseases and the underlying mechanisms,with special emphasis on structure-activity relationships.This focus is particularly important because the structural characteristics of DOP are highly dependent on extraction and analytical methods,which have contributed to inconsistencies in pharmacological findings over the past two decades.Finally,the review highlights recent advances in the understanding of the in vivo biosynthesis and regulatory mechanisms of themajor bioactive components inDO,with a particular focus onmolecular regulation and responses to agricultural interventions.These factors are critical for the production of high-quality DO.Overall,this study develops a comprehensive knowledge framework that connects DO’s traditional applications of DO to its two key research areas:pharmacological functions and quality formation.We anticipate this framework will offer clear guidance for future research from a clinical perspective.
文摘Background:Skin photoaging is a physiological or pathological process caused by multiple factors.Developing anti-skin photoaging drugs is a hot topic in cosmetology research fields.The purpose of this study was to explore the therapeutic effect of Dendrobium officinale(D.officinale)on skin aging.Methods:The ingredients of D.officinale were detected by UHPLC-Q-TOF/MS.The targets of D.officinale were screened by Swiss Target Prediction database.GeneCards,NCBI,and OMIM databases were utilized to find out the targets associated with skin photoaging.Overlapping targets of D.officinale and skin photoaging were obtained by Venn analysis.The ingredient-disease target network and protein-protein interaction network were constructed by using the STRING database and Cytoscape software.The key compounds and hub genes were obtained by analyzing networks.The DAVID database was applied for Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analysis of the overlapping targets.Autodock Vina software was used to simulate molecular docking and the results were visualized using Pymol.Finally,the skin photoaging models of cells and mice were established to validate the results predicted by network pharmacology.Results:In D.officinale,a total of 59 compounds and 595 targets were detected,of which 59 proteins were intersectional with skin photoaging targets.The top 10 active ingredients(Dendrophenol,Herbacetin,Lyoniresinol,Trans-ferulaldehyde,Naringenin,and so on)and 8 hub genes(AKT1,TNF,VEGFA,MAPK3,CASP3,MMP9,CTNNB1,and EGFR)were identified.All the key active compounds could bind well with core protein targets(binding energy<-5 kcal/mol).The potential therapeutic targets were related to the response to reactive oxygen species,collagen catabolic process,extracellular matrix organization,and apoptotic process,mainly.We also found that D.officinale could enhance the cell viability and activity of anti-oxidases,reduce reactive oxygen species and MMP9 levels,and stable mitochondrial membrane potential.Furthermore,D.officinale could alleviate skin photoaging injury and reduce malondialdehyde level in mice.Conclusion:D.officinale alleviated skin photoaging via regulating oxidative stress,apoptosis,and collagen catabolic process.
基金supported by the National Natural Science Foundation of China(82274134)the National Key R&D Plan(2017YFC1702200,2017YFC1702202)+1 种基金Key R&D Program Projects in Zhejiang Province(2020C04020)the Key Research and Development Program of Zhejiang Province(2025C02183).
文摘The aim of this study was to investigate the role of Dendrobium officinale crude polysaccharides(DOPS)and D.officinale purified polysaccharides(DOPS100)in attenuating immune dysfunction in subfertile mice.The results of the study revealed several noteworthy findings.First,DOPS and DOPS100 treatments led to significant improvement in weight gain and reversal of fatigue-related behaviors compared to the normal group.Second,both DOPS and DOPS100 showed effectiveness in reducing immune organ swelling,modulating immunoglobulin A(IgA)and immunoglobulin M(IgM)levels,and restoring complement(C3c and C4)levels.In addition,they demonstrated a significant ability to enhance the integrity of the intestinal mechanical barrier by differentially upregulating the tight junction proteins Occludin and Zonula occludens 1(ZO-1).In addition,it was found that DOPS100 specifically enhanced the CD4^(+)-T helper 17 cell(Th17)/regulatory T cell(Treg)immune axis in the gut,as evidenced by increased expression of forkhead box protein 3(Foxp3)as well as decreased expression of retinoic acid receptor-related orphan receptor γt(RORγt),and further modulation of interleukin 10(IL-10),interleukin 22(IL-22),and interleukin 17A(IL-17A)expression levels of inflammatory factors.These findings collectively suggest that DOPS100 holds significant potential in improving sub-healthy status by repairing the intestinal barrier,restoring local immune homeostasis,and activating the intestinal immune regulatory network.The study’s outcomes provide valuable insights into the therapeutic implications of DOPS and DOPS100 in addressing immune dysfunction in sub-healthy conditions.
文摘To study the antioxidant constituents from the stems of Dendrobium nobile, and to discuss theft structure-activity relationship. Compounds were isolated from a 60% ethanolic extract by various chromatographic techniques and were identified by spectral analysis. The antioxidant activities of compounds were evaluated by DPPH free radical scavenging assay. Five phenanthrenes and four lignans were obtained from the active fractions ofD. nobile. Their structures were identified as fimbriatone （1）, confusarin （2）, flavanthrinin （3）, 2,5-dihydroxy-4,9-dimethoxyphenanthrene （4）, 3,7-dihydroxy-2,4-dimethoxyphenanthrene （5）, syringaresinol （6）, pinoresinol （7）, medioresinol （8） and lirioresinol-A （9）, respectively. Compounds 2 and 6 exhibited more potent DPPH scavenging activities than vitamin C. All the above compounds were reported from this plant for the first time, and compounds 3, 4 and 9 were reported for the first time from the genus of Dendrobiurn. For all phenanthrenes and lignans, an electron-donating methoxyl group in the ortho position to the phenolic hydroxyl group exhibits enhanced antioxidant activities.
基金Supported by the Scientific Research Project of Neijiang Normal Unverisity(No:07NJZ-03)~~
文摘[Objective] This study aimed to explore the inoculation effects of Dendrobium officinale mycorrhizal fungi on their plantlets. [Method] Endophytic strains Tj1, Tj2 and Tj3 were obtained by isolation and purification from mycorrhiza of wild Dendrobium officinale and inoculated on the root system of Dendrobium officinale for inoculation test. [Result] Under tissue-culture conditions, at early stage, Tj1 strain hadn＇t shown promotion effect on Dendrobium officinale, Tj2 strain had shown relatively strong promotion effects, and Tj3 strain had promoted the growth of roots; at late stage, Tj1 strain had shown relatively strong promotion effects, Tj2 strain had shown the best inoculation effects and the strongest promotion effects, while Tj3 strain had caused root and seedling rot problems of the plantlets; under outdoor conditions, after inoculation with Tj2 strain, the number of leaves and lateral buds were increased, the growth of lateral root and the increase of plant height were significant, the leaves of Dendrobium officinale plantlets were large and dark green and an obvious root enlargement phenomenon was observed. [Conclusion] The two inoculation methods both indicate that Tj2 strain has relatively strong promotion effects on the growth of Dendrobium officinale roots and shoots, the increase of plant number and plant height, and the germination of new shoots and roots, which proved the effective establishment of symbiotic relationship between Tj2 strain and Dendrobium officinale. Therefore, T2 strain has practical application values on the successful cultivation of Dendrobium officinale plantlets.
基金Supported by Plan Project of Science and Technology Committee of Guangxi Province (0322024-3A) Scientific Research Foundation for Returned Scholars of Guangxi Province~~
文摘[Objective]The aim was to select the suitable hormone factors for flowering induction in vitro culture of Dendrobium officinate Kimura et Migo.[Method]The test-tube plantlets from the stems of Dendrobium officinate Kimura et Migo were used as the experimental materials and MS medium as the basic medium.Comparative tests have been done between single-factor hormone treatments（different concentrations of PP333 or TDZ） and multi-factor hormone treatments（different combinations of PP333,TDZ,6-BA and NAA） to research the effects of hormone factors on the flowering induction of the plantlets.[Result]Among the single-factor hormone treatments,the suitable concentration and the rate of flower buds formation of PP333 treatment were 0.2 mg/L and 8.5%,the that of TDZ treatment were 0.06 mg/L and 15.5%;the effects of multi-factor hormone treatments on the flowering induction were ordered as follow：（PP333 ＋ 6-BA ＋ NAA ＋ TDZ）〉 （PP333 ＋ 6-BA ＋ NAA）〉 （PP333 ＋ 6-BA） and（PP333 ＋ NAA） ;the most suitable treatment was PP333 0.3 mg/L ＋ 6-BA 0.5 mg/L ＋ NAA 0.5 mg/L ＋ TDZ 0.06 mg/L,the rate of flower bud formation and the rate of the blossomed flower were respectly reached to 80.4% and 90.3%.[Conclusion]PP333 and TDZ showed the important effect on the flowering induction in vitro culture of Dendrobium officinate Kimura et Migo.The effect of TDZ was better than that of PP333.It is much more conducive to the flower bud formation,when using appropriate concentration of TDZ combined with other hormones properly.
基金Supported by the Hainan Natural Science Foundation(312024)Special Fund for Scientific Research in the Public Interest,China(201203071)~~
文摘[Objective] This study aimed to analyse the genetic diversity of and genet- ic relationship between 33 Dendrobium species by using RSAP （Restriction Site Amplification Polymorphism） marker technique. [Method] A PCR-reaction system was established, by which 33 pairs of primers screened from 45 pairs were used to con- duct the PCR amplification. [Results] The 33 pairs of primers produced 2 047 ampli- fied fragments, of which 2 044 were polymorphic, with a polymorphism rate of 99.8%, and each pair of primers produced 61.9 polymorphic fragments on average. The genetic similarity coefficients between species ranged from 0.081 to 0.442, sug- gesting that the experimental materials used in this study possess a wide genetic background. According to the result of RSAP, the 33 Dendrobium species were di- vided into 6 groups by the cluster analysis （UPGMA）. The result showed that the genetic relationship between D. acinaciforme and D. guangxiense was the closest, and that between D. sinense and either D. lituiflorum or D. hancockii was the fur- thest. [Conclusion] The result suggested that Dendrobium germplasms have a great genetic diversity. This study provides a molecular basis for the classification and breeding of Dendrobium plants, as well as the selection of hybridization parents in the future.
文摘[Objective] This research aimed to establish an inductively coupled plasma mass spectrometry （ICP-MS） method for determination of five kinds of heavy metals （Pb, Cd, Cu, As and Hg） in Dendrobium candidium Wall. ex Lindl. [Method] The samples were digested in HNO3-H202 acids system by closed vessel microwave di- gestion. At the same time, internal standard was added to avoid the matrix effect. [Result] The five kinds of heavy meals all showed good linear relationships, and the correlation coefficients ranged from 0.998 2 to 0.999 9. The detection limits of the five kinds of heavy metals were in the range of 0.008-0.750 μg/L, while the stan- dard recovery rates were in the range of 90.4%-96.9%. The relative standard devi- ations ranged from 1.6% to 8.2%. The heavy metals in the Ginseng and Astragalus national standard samples were determined by the established ICP-MS method, and the results were in conformity with the standard values. [Conclusion] The established method has simple and convenient operation and accurate and reliable results, and it meets the requirements by determination of heavy metals in Dendrobium candididium Wall. ex Lindl.
文摘[Objective] To optimize the techniques for culturing Cr-enriched Dendrobi-um candidum protocorm. [Method] By adopting Plackett-Burman test, the effects of time, temperature, Cr-content in medium, NAA content, KT content and light intensi-ty on Cr-enriched protocorm were researched. Three factors of time, Cr-content in medium and light intensity had statistical y significant effects; then steepest ascent procedures were applied to define optimal response region of these three factors; fi-nal y the optimal factors were determined by RSM analysis. [Result] The results showed that the optimal conditions for Cr-enrichment were chromium concentration at 0.37 mg/L, culture time of 59 d and il umination intensity of 1 822.22 lx. The predicted value of Cr-content in protocorm was 5.08 mg/kg. [Conclusion] RSM can optimize the techniques for culturing Cr-enriched protocorm of Dendrobium can-didum, provide the optimum process parameters and lay the foundation for further research.
基金Supported by Fundamental Research Funds for Guangxi Academy of Sciences(13YJ22SWS20)~~
文摘In summer, Cha No. 39 of 292 bags and 205 of 244 bags were inter- planted with Dendrobium officinale on cultivation bed, respectively, whose yields and biological efficiencies reached 102.46 kg and 103.98%, and 96.77 kg and 113.32%, accordingly. The seedlings of Dendrobium officinale interplanted with Pleurotus os- treatus grew by 2.78 cm during 10 weeks on cultivation beds, and the seedlings grew by 2.68 cm in the cultivation beds without interplanting arrangement. These demonstrated that the interplanting of Dendrobium officinale and Pleurotus ostreatus has none adverse effects on growth of Dendrobium officinale.
基金Supported by National Nonprofit Institute Research Grant of CATAS-TCGRI(1630032014027)Special Fund for Agro-scientific Research in the Public Interest(201203071)Special Project for Scientific and Technological Achievements Demonstration and Promotion in Hainan Province(SQ2013CGSF0006)~~
文摘To optimize the technique of rapid propagation of Dendrobium hybrida seedlings and to explore a hormone-free tissue culture method for D.hybrida,six kinds of mycorrhizal fungi which were isolated from the wild orchids were made into fungal elicitors.These fungal elicitors were added into the DE medium with concentrations of 40,60 and 80 ml/L,respectively.After a 90-d culturing,the effects of fungal elicitors on the growth of D.hybrida cultivar ‘088＇ tissue culture seedlings were studied.The results showed that treatment 13（T13） extremely significantly increased the fresh weight,but other treatment groups had no significant effects.In addition,T1,T5,T9,T11 and T13 extremely significantly influenced the contents of chlorophyll a and chlorophyll a ＋ b.However,T1 and T11 had extremely significantly effect on the content of chlorophyll b.Combining the effects on fresh weight and chlorophyll content,it could be concluded T13（40 ml/L of Y05） has promoting effects on the growth of D.hybrida tissue culture seedlings.
基金supported by the Three Biological Technology Co.,LTD in Tai’an,Shandong Province.
文摘T o investigate cold tolerance of different cultivars and species in Dendrobium Sw. in North China, 8 strains of Dendrobium officinal and other 5 Dendrobium species were used to compare their cold-hardiness index in this study. The content of electrolyte leakage was increasing in Dendrobium Sw. as the temperature decreasing, while the content of peroxidase(POD) activity and the soluble sugar showed a rising trend before a downward trend. Besides, external morphology was another evaluation standard of cold tolerance. Thus, there was a significant difference between Dendrobium officinal and other Dendrobium species in the cold tolerance, and their cold tolerance capacity was as follows: s1 > s100 >s13 > 1301 > s300 > Dendrobium nobile > s126 > s145 > s95 > Dendrobium lindleyi > Dendrobium chrysotoxum > Dendrobium thyrsiflorum > Dendrobium lohohense.
文摘A new fluorenone derivative, named chrysotoxone(3) was isolated from the ethanolic extract of the stems of Dendrobium chrysotoxum (Orchidaceae), along with three known compounds. The structure of the new fluorenone was elucidated as 2,5,8trihydroxy1,4dimethoxy9fluorenone.
