Biopolymer core-shell microspheres play a crucial role in various biomedical applications,including drug delivery,tissue engineering,and diagnostics.These applications require microparticles with consistent,well-contr...Biopolymer core-shell microspheres play a crucial role in various biomedical applications,including drug delivery,tissue engineering,and diagnostics.These applications require microparticles with consistent,well-controlled size and precise shape fidelity.However,achieving high-throughput synthesis of size and shape-controlled core-shell biopolymer microgels remains a significant challenge.Herein,we present a one-step process for the high-throughput generation of monodisperse,luminescent,chitosan alginate core-shell microspheres by a novel manipulation of a centrifugal microfluidic device.We utilized the pH sensitivity of chitosan and the ionic gelation properties of alginate to create well-defined core-shell morphologies.To address particle merging issues and promote uniform particle size generation,we introduced an innovative pulsed mode operation in our centrifugal microfluidics device.We also incorporated fluorescent,nitrogen-functionalized graphene quantum dots into the core-shell structures,thereby rendering them useful for real-time imaging,which is necessary for diagnostic and therapeutic applications.To enhance biocompatibility,the alginate solution was supplemented with fish gelatin(FG).The resulting microspheres exhibited excellent structural integrity maintaining their core-shell structure after 15 days.Biocompatibility was demonstrated by C2C12 cell viability exceeding 88%after 15 days and by bacterial viability reaching the same percentage after 2 days.The system demonstrates considerable scalability,allowing for the consistent production of large quantities of microspheres without compromising functionality.The streamlined and efficient methodology simplifies the production process while unlocking new possibilities in targeted therapies,tissue regeneration,and diagnostics.展开更多
基金support of CONAHCYT(Consejo Nacional de Humanidades,Ciencias y Tecnologías,México)in the form of Graduate Program Scholarships and the support by Tecnológico de Monterrey in the form of tuition fee waiverthe funding provided by CONAHCYT in the form of scholarship as member of the National System of Researchers(SNI 1047863)+2 种基金he financial support of Federico Baur Endowed Chair in Nanotechnology(ILST002-23ID69001)the funding provided by CONAHCYT in the form of scholarship as a member of the National System of Researchers(CVU:969467)the financial support of FEMSA foundation.
文摘Biopolymer core-shell microspheres play a crucial role in various biomedical applications,including drug delivery,tissue engineering,and diagnostics.These applications require microparticles with consistent,well-controlled size and precise shape fidelity.However,achieving high-throughput synthesis of size and shape-controlled core-shell biopolymer microgels remains a significant challenge.Herein,we present a one-step process for the high-throughput generation of monodisperse,luminescent,chitosan alginate core-shell microspheres by a novel manipulation of a centrifugal microfluidic device.We utilized the pH sensitivity of chitosan and the ionic gelation properties of alginate to create well-defined core-shell morphologies.To address particle merging issues and promote uniform particle size generation,we introduced an innovative pulsed mode operation in our centrifugal microfluidics device.We also incorporated fluorescent,nitrogen-functionalized graphene quantum dots into the core-shell structures,thereby rendering them useful for real-time imaging,which is necessary for diagnostic and therapeutic applications.To enhance biocompatibility,the alginate solution was supplemented with fish gelatin(FG).The resulting microspheres exhibited excellent structural integrity maintaining their core-shell structure after 15 days.Biocompatibility was demonstrated by C2C12 cell viability exceeding 88%after 15 days and by bacterial viability reaching the same percentage after 2 days.The system demonstrates considerable scalability,allowing for the consistent production of large quantities of microspheres without compromising functionality.The streamlined and efficient methodology simplifies the production process while unlocking new possibilities in targeted therapies,tissue regeneration,and diagnostics.
