In this study,we tested the ability of sodium dehydroacetate(SD)to extend the shelf-life of‘Kyoho’grape.Among the different concentrations of SD tested(0,0.01,0.1 and 1.0 mmol L^(-1)),0.01 mmol L^(-1) SD was the mos...In this study,we tested the ability of sodium dehydroacetate(SD)to extend the shelf-life of‘Kyoho’grape.Among the different concentrations of SD tested(0,0.01,0.1 and 1.0 mmol L^(-1)),0.01 mmol L^(-1) SD was the most effective in prolonging the shelf-life of‘Kyoho’grape.Compared with the control,the weight loss rate,browning index and hydrogen peroxide(H_(2)O_(2))and malonaldehyde contents were significantly lower in the 0.01 mmol L^(-1) SD treatment,whereas the healthy berry rate,berry firmness,total soluble solids(TSS)content,ascorbic acid content and superoxide dismutase(SOD)activity were significantly higher.In addition,an analysis of‘Kyoho’grape DNA using methylation sensitive amplification polymorphism(MSAP)markers showed that the average DNA methylation level was significantly higher in the 0.01 mmol L^(-1) SD treatment than in the control.Together,these results indicate that 0.01 mmol L^(-1) SD could be used to extend the shelf-life of‘Kyoho’grape.Moreover,a strong connection between reactive oxygen species(ROS)metabolism and DNA methylation change during storage was revealed.展开更多
Objective The present study was undertaken to evaluate the subchronic oral toxicity of sodium dehydroacetate(DHA-Na)and to determine the point of departure(POD),which is a critical factor in the establishment of an ac...Objective The present study was undertaken to evaluate the subchronic oral toxicity of sodium dehydroacetate(DHA-Na)and to determine the point of departure(POD),which is a critical factor in the establishment of an acceptable dietary intake.Methods DHA-Na was administered once daily by gavage to Sprague–Dawley rats at dose levels of 0.0,31.0,62.0,and 124.0 mg/kg BW per day for 90 days,followed by a recovery period of 4 weeks in the control and 124.0 mg/kg BW per day groups.The outcome parameters were mortality,clinical observations,body weights,food consumption,hematology and clinical biochemistry,endocrine hormone levels,and ophthalmic,urinary,and histopathologic indicators.The benchmark dose(BMD)approach was applied to estimate the POD.Results Significant decreases were found in the 62.0 and 124.0 mg/kg BW groups in terms of the body weight and food utilization rate,whereas a significant increase was found in the thyroid stimulating hormone levels of the 124.0 mg/kg BW group.Importantly,the 95%lower confidence limit on the BMD of 51.7 mg/kg BW was modeled for a reduction in body weight.Conclusion The repeated-dose study indicated the slight systemic toxicity of DHA-Na at certain levels(62.0 and 124.0 mg/kg BW)after a 90-day oral exposure.展开更多
Many authorized food additives can affect host health,including cause chronic inflammation and metabolic disorders by influencing the gut microbiota.Sodium dehydroacetate(DHA-S),an approved yet controversial food pres...Many authorized food additives can affect host health,including cause chronic inflammation and metabolic disorders by influencing the gut microbiota.Sodium dehydroacetate(DHA-S),an approved yet controversial food preservative with strong antimicrobial properties,was globally used in multiple industries,but its influence on gut microbiota and host health remains unsettled.This study revealed that DHA-S significantly stimulated food intake but retarded body weight gain in mice,depending on the existence of gut microbiota.DHA-S-induced hyperphagia was mainly responsible for appetite-related hormonal responses,including glucagon-like peptide 1 and peptide YY.Notably,DHA-S induced lipid metabolic disturbance and low-grade local and systemic inflammation in normal mice.These adverse effects,particularly increased total cholesterol and decreased interleukin-10(IL-10),could be transferred to recipient mice that received DHA-S-modulated microbiota via fecal microbiota transplantation(FMT).Moreover,DHA-S extensively modified the gut microbiota’s pattern and function toward a pro-inflammatory profile,resulting in increased Firmicutes,decreased Bacteroidota,and reduced production of butyric acid,which further caused undesired health outcomes in normal mice.These microbial patterns and metabolic functions were not only retained during a 5-week recovery period by switching from a DHA-S-containing diet to a basal diet,but also recurred in recipient mice following FMT of DHA-S-modulated microbiota.Overall,this research is to raise caution about the overeating disorder,growth retarda-tion and pro-inflammatory effects of DHA-S and to establish a guide for its proper use,especially for the pop-ulation with a history of antibiotics treatment.展开更多
To ensure the safety of consumers, prolong the shelf life of moon cake and reduce the cost of manufacturing and marketing enterprises, the optimization of preservative formula and the shelf life of cantonese style moo...To ensure the safety of consumers, prolong the shelf life of moon cake and reduce the cost of manufacturing and marketing enterprises, the optimization of preservative formula and the shelf life of cantonese style moon cake were studied. Through single and compound formula preservative test, the results showed that potassium sorbate and sodium dehydroacetate could inhibit the microorganisms in the moon cake, but the inhibitory effect of sodium dehydroacetate was stronger than that of potassium sorbate; and the combination of potassium sorbate and sodium dehydroacetate had a synergistic effect, and the best formula is ＇potassium sorbate 0.44 g/kg mixed with sodium dehydroacetate 0.38 g/kg. A batch of moon cake was prepared with the best preservative formula, then the total number of colonies, molds and coliform group in the samples were detected by random sampling at irregular intervals. The total number of molds exceeded the limit on the 187^th d, and it indicated that the shelf life of the product was 187 d. Compared with the conventional 15-day shelf life, this research greatly prolongs the sheff life of cantonese style moon cake, and cut down the cost of moon cake enterprises. It is worthy of popularization and application.展开更多
A series of new mixed ligand complexes of cobalt(II), nickel(II), copper(II) and cadmium(II) have been synthesized with 3benzyl1H4[(2 methoxybenzylidine) amino]1, 2, 4triazole5 thione (MBT), 3bezyl1H4[(4chlorobenzylid...A series of new mixed ligand complexes of cobalt(II), nickel(II), copper(II) and cadmium(II) have been synthesized with 3benzyl1H4[(2 methoxybenzylidine) amino]1, 2, 4triazole5 thione (MBT), 3bezyl1H4[(4chlorobenzylidine) amino]1, 2, 4triazole5thione (CBT), 3benzyl 1H4[(4nitrobenzylidine)amino]1, 2, 4triazole 5thione (NBT) and dehydroacetic acid sodium salt (Nadha). The mixed ligand complexes have been characterized by elemental analysis, spec troscopic spectral measurements (IR, UVVis.), molar conductance, magnetic measurements and thermal studies. The stoichiometry of these com plexes is M:L1:L2 = 1:1:1, 1:2:1 or 1:1:2 where L1 = NBT, CBT and MBT and L2 = Nadha. Tetrahedral structure was proposed for all Cd(II) mixed ligand complexes while the square planar geometry was proposed for Cu(II) mixed ligand complex with NBT. Octahedral structure was proposed for Ni(II), Co(II) mixed ligand complexes and Cu(II) mixed ligand complexes with CBT and MBT ligands. The thermal decomposition study of the prepared complexes was monitored by TG, DTG and DTA analysis in dynamic nitrogen atmosphere. TG, DTG and DTA studies confirmed the chemical formulations of theses complexes. The kinetic parameters were determined from the the thermal decomposition data using the graphical methods of CoatsRedfern and HorwitzMetzger. Thermodynamic parameters were calculated using standard relations.展开更多
基金financially supported by the National Natural Science Foundation of China(U1904113)the National Key Research and Development Program of China(2018YFD1000105)+1 种基金the Program for Innovative Research Team(in Science and Technology)in University of Henan Province,China(21IRTSTHN021)the Program for Science&Technology Innovation Talents in Universities of Henan Province,China(21HASTIT035)。
文摘In this study,we tested the ability of sodium dehydroacetate(SD)to extend the shelf-life of‘Kyoho’grape.Among the different concentrations of SD tested(0,0.01,0.1 and 1.0 mmol L^(-1)),0.01 mmol L^(-1) SD was the most effective in prolonging the shelf-life of‘Kyoho’grape.Compared with the control,the weight loss rate,browning index and hydrogen peroxide(H_(2)O_(2))and malonaldehyde contents were significantly lower in the 0.01 mmol L^(-1) SD treatment,whereas the healthy berry rate,berry firmness,total soluble solids(TSS)content,ascorbic acid content and superoxide dismutase(SOD)activity were significantly higher.In addition,an analysis of‘Kyoho’grape DNA using methylation sensitive amplification polymorphism(MSAP)markers showed that the average DNA methylation level was significantly higher in the 0.01 mmol L^(-1) SD treatment than in the control.Together,these results indicate that 0.01 mmol L^(-1) SD could be used to extend the shelf-life of‘Kyoho’grape.Moreover,a strong connection between reactive oxygen species(ROS)metabolism and DNA methylation change during storage was revealed.
基金supported by the National Key R&D Program of China[2019YFC1605203]China Food Safety Talent Competency Development Initiative:CFSA 523 Program
文摘Objective The present study was undertaken to evaluate the subchronic oral toxicity of sodium dehydroacetate(DHA-Na)and to determine the point of departure(POD),which is a critical factor in the establishment of an acceptable dietary intake.Methods DHA-Na was administered once daily by gavage to Sprague–Dawley rats at dose levels of 0.0,31.0,62.0,and 124.0 mg/kg BW per day for 90 days,followed by a recovery period of 4 weeks in the control and 124.0 mg/kg BW per day groups.The outcome parameters were mortality,clinical observations,body weights,food consumption,hematology and clinical biochemistry,endocrine hormone levels,and ophthalmic,urinary,and histopathologic indicators.The benchmark dose(BMD)approach was applied to estimate the POD.Results Significant decreases were found in the 62.0 and 124.0 mg/kg BW groups in terms of the body weight and food utilization rate,whereas a significant increase was found in the thyroid stimulating hormone levels of the 124.0 mg/kg BW group.Importantly,the 95%lower confidence limit on the BMD of 51.7 mg/kg BW was modeled for a reduction in body weight.Conclusion The repeated-dose study indicated the slight systemic toxicity of DHA-Na at certain levels(62.0 and 124.0 mg/kg BW)after a 90-day oral exposure.
基金supported by National Natural Science Foundation of China(Grant No.32072224)Zhejiang Agricultural and Forestry University Scientific Research Development Fund Project(Grant No.2024LFR024).
文摘Many authorized food additives can affect host health,including cause chronic inflammation and metabolic disorders by influencing the gut microbiota.Sodium dehydroacetate(DHA-S),an approved yet controversial food preservative with strong antimicrobial properties,was globally used in multiple industries,but its influence on gut microbiota and host health remains unsettled.This study revealed that DHA-S significantly stimulated food intake but retarded body weight gain in mice,depending on the existence of gut microbiota.DHA-S-induced hyperphagia was mainly responsible for appetite-related hormonal responses,including glucagon-like peptide 1 and peptide YY.Notably,DHA-S induced lipid metabolic disturbance and low-grade local and systemic inflammation in normal mice.These adverse effects,particularly increased total cholesterol and decreased interleukin-10(IL-10),could be transferred to recipient mice that received DHA-S-modulated microbiota via fecal microbiota transplantation(FMT).Moreover,DHA-S extensively modified the gut microbiota’s pattern and function toward a pro-inflammatory profile,resulting in increased Firmicutes,decreased Bacteroidota,and reduced production of butyric acid,which further caused undesired health outcomes in normal mice.These microbial patterns and metabolic functions were not only retained during a 5-week recovery period by switching from a DHA-S-containing diet to a basal diet,but also recurred in recipient mice following FMT of DHA-S-modulated microbiota.Overall,this research is to raise caution about the overeating disorder,growth retarda-tion and pro-inflammatory effects of DHA-S and to establish a guide for its proper use,especially for the pop-ulation with a history of antibiotics treatment.
基金Supported by Project of the Department of Education of Fujian Province(JB13317)~~
文摘To ensure the safety of consumers, prolong the shelf life of moon cake and reduce the cost of manufacturing and marketing enterprises, the optimization of preservative formula and the shelf life of cantonese style moon cake were studied. Through single and compound formula preservative test, the results showed that potassium sorbate and sodium dehydroacetate could inhibit the microorganisms in the moon cake, but the inhibitory effect of sodium dehydroacetate was stronger than that of potassium sorbate; and the combination of potassium sorbate and sodium dehydroacetate had a synergistic effect, and the best formula is ＇potassium sorbate 0.44 g/kg mixed with sodium dehydroacetate 0.38 g/kg. A batch of moon cake was prepared with the best preservative formula, then the total number of colonies, molds and coliform group in the samples were detected by random sampling at irregular intervals. The total number of molds exceeded the limit on the 187^th d, and it indicated that the shelf life of the product was 187 d. Compared with the conventional 15-day shelf life, this research greatly prolongs the sheff life of cantonese style moon cake, and cut down the cost of moon cake enterprises. It is worthy of popularization and application.
文摘A series of new mixed ligand complexes of cobalt(II), nickel(II), copper(II) and cadmium(II) have been synthesized with 3benzyl1H4[(2 methoxybenzylidine) amino]1, 2, 4triazole5 thione (MBT), 3bezyl1H4[(4chlorobenzylidine) amino]1, 2, 4triazole5thione (CBT), 3benzyl 1H4[(4nitrobenzylidine)amino]1, 2, 4triazole 5thione (NBT) and dehydroacetic acid sodium salt (Nadha). The mixed ligand complexes have been characterized by elemental analysis, spec troscopic spectral measurements (IR, UVVis.), molar conductance, magnetic measurements and thermal studies. The stoichiometry of these com plexes is M:L1:L2 = 1:1:1, 1:2:1 or 1:1:2 where L1 = NBT, CBT and MBT and L2 = Nadha. Tetrahedral structure was proposed for all Cd(II) mixed ligand complexes while the square planar geometry was proposed for Cu(II) mixed ligand complex with NBT. Octahedral structure was proposed for Ni(II), Co(II) mixed ligand complexes and Cu(II) mixed ligand complexes with CBT and MBT ligands. The thermal decomposition study of the prepared complexes was monitored by TG, DTG and DTA analysis in dynamic nitrogen atmosphere. TG, DTG and DTA studies confirmed the chemical formulations of theses complexes. The kinetic parameters were determined from the the thermal decomposition data using the graphical methods of CoatsRedfern and HorwitzMetzger. Thermodynamic parameters were calculated using standard relations.
