Dehydrin(DHN)enhances plant resistance to environmental stress by regulating the synthesis of osmotic adjustment substances and scavenging reactive oxygen species.However,the role of PbDHN3 under salt stress remains u...Dehydrin(DHN)enhances plant resistance to environmental stress by regulating the synthesis of osmotic adjustment substances and scavenging reactive oxygen species.However,the role of PbDHN3 under salt stress remains unclear.In this study,salt stress induced high expression of PbDHN3,and the overexpression of PbDHN3(OE-PbDHN3)enhanced plant growth under salt stress compared to wild-type(WT)plants.OE-PbDHN3 plants exhibited higher chlorophyll content and root growth capacity than WT plants under salt stress.Transcriptome analysis revealed that PbDHN3 expression is associated with ethylene signaling pathways.OE-PbDHN3 transgenic plants substantially influenced ethylene content and the expression of related genes.Following treatment with exogenous ethephon,the transgenic lines notably inhibited the processes of ethylene synthesis and signaling transduction.OE-PbDHN3 transgenic lines treated with exogenous ethylene and the ethylene inhibitor 1-MCP demonstrated significant inhibition of ethylene synthesis and signaling transduction,while promoting root development and chlorophyll content.Under salt stress,OE-PbDHN3 downregulated the expression of ethylene biosynthesis genes PbACO1-like and PbACO2,and signal transduction genes PbEIN3-like during the initial stress phase.This early regulation mitigated the adverse effects of salt stress on the plants.These findings demonstrate that PbDHN3 ameliorates the ethylene-mediated plant growth phenotype under salt stress through regulation of ethylene synthesis and signal transduction.展开更多
A full-length cDNA of dehydrin BcDh2 from Boea crassifolia and its antisense nucleotidesequence have been transferred into tobacco (Nicotiana tabacum) NC89 under the controlof a caulifower mosaic virus 35S promoter. U...A full-length cDNA of dehydrin BcDh2 from Boea crassifolia and its antisense nucleotidesequence have been transferred into tobacco (Nicotiana tabacum) NC89 under the controlof a caulifower mosaic virus 35S promoter. Under a progressive water stress, photosyntheticrate, transpiration rate and stomatal conductance of the sense and antisense plantsreduced, and those of the control reduced much more. Photosynthetic rate, transpirationrate and stomatal conductance of all plants tested increased significantly 24 hourslater after recoveried water supply, and those of the sense and antisense plants werehigher than control. These indicated that overexpression of a dehydrin gene in tobaccomay improve tolerance to water stress for plants, however, antisense BcDh2 gene intransgenic plant did not influence physiological conditions. The results of germinationexperiment of the transgenic seeds showed that on MS medium with different concentrationPEG (8000), sense seed could more endure drought than control, while antisense seed wassensitive to drought. The results suggested that the overexpression of a dehydrin genein tobacco might improve the tolerance to water stress for plants.展开更多
Drought is one of environmental stresses which the most limiting to plant growth and productivity. Drought stress led to a series of changes including biochemical changes like accumulation of osmolit and specific prot...Drought is one of environmental stresses which the most limiting to plant growth and productivity. Drought stress led to a series of changes including biochemical changes like accumulation of osmolit and specific proteins involved in stress tolerance. One of the proteins that play a role in the mechanism of drought resistance is dehydrin protein. This study aimed to identify the protein profiles and dehydrin accumulation in 7 varieties of local Indonesian soybeans: Tanggamus, Nanti, Seulawah and Tidar (tolerant), Wilis and Burangrang (moderate) and Detam-1 (drought stress sensitive). Plants were treated with drought stress by adjusting soil water content to 25% below field capacity and compared with plants which were grown on normal condition as control plants. The results of SDS-PAGE electrophoresis showed a new protein with the molecular weight of 13 and 52 kDa were induced in Tanggamus, Nanti, Seulawah and Tidar varieties. Western blotting analysis for dehydrin showed that the quantity of the protein in the leaves of all varieties except Tanggamus decreased in drought stress conditions. The quantity of dehydrin protein in tolerant varieties higher than the protein quantity in both moderate varieties and drought sensitive.展开更多
Dehydrins(DHNs),as members of the late embryogenesis abundant protein family,play critical roles in the protection of seeds from dehydration and plant adaptation to multiple abiotic stresses.Vitrification is a basic m...Dehydrins(DHNs),as members of the late embryogenesis abundant protein family,play critical roles in the protection of seeds from dehydration and plant adaptation to multiple abiotic stresses.Vitrification is a basic method in plant cryopreservation and is characterized by forming a glassy state to prevent lethal ice crystals produced during cryogenic storage.In this study,ApSK3 type DHN was genetically transformed into embryogenic calluses(EC)of Agapanthus praecox by overexpression(OE)and RNA interference(RNAi)techniques to evaluate the in vivo protective effect of DHNs during cryopreservation.The cell viability showed a completely opposite trend in OE and RNAi cell lines,the cell relative death ratio was decreased by 20.0%in ApSK3-OE EC and significantly increased by 66.15%in ApSK3-RNAi cells after cryopreservation.Overexpression of ApSK3 increased the content of non-enzymatic antioxidants(AsA and GSH)and up-regulated the expression of CAT,SOD,POD,and GPX genes,while ApSK3-RNAi cells decreased antioxidant enzyme activities and FeSOD,POD,and APX genes expression during cryopreservation.These findings suggest that ApSK3 affects ROS metabolism through chelating metal ions(Cu^(2+)and Fe^(3+)),alleviates H_(2)O_(2)and OH·excessive generation,activates the antioxidant system,and improves cellular REDOX balance and membrane lipid peroxidation damage of plant cells during cryopreservation.DHNs can effectively improve cell stress tolerance and have great potential for in vivo or in vitro applications in plant cryopreservation.展开更多
A 500-bp cDNA fragment was amplified via RT-PCR from drought-induced total RNA of the drought-tolerant B. crassifolia Hemsl. using primers based on the sequence of published dehydrin conserved region. By using 5’ RAC...A 500-bp cDNA fragment was amplified via RT-PCR from drought-induced total RNA of the drought-tolerant B. crassifolia Hemsl. using primers based on the sequence of published dehydrin conserved region. By using 5’ RACE, full-length coding region (1 148 bp) of BDN1 gene was produced. It is a new member of the dehydrin gene family. Southern analysis indicated that BDN1 is present in the 6. crassifolia genome as a single-copy gene. Northern analysis revealed that its expression is inducible by drought and cold stresses as well as ABA application.展开更多
油莎豆播种期长、产量高,是目前已知唯一在块茎器官中积累大量油脂的特种经济作物,具有广阔的综合利用前景。为了进一步提高油莎豆的品质和产量,积极筛选和鉴定相关的抗性基因,研究基于转录组数据,以油莎豆热研1号为材料,采用RT-PCR技...油莎豆播种期长、产量高,是目前已知唯一在块茎器官中积累大量油脂的特种经济作物,具有广阔的综合利用前景。为了进一步提高油莎豆的品质和产量,积极筛选和鉴定相关的抗性基因,研究基于转录组数据,以油莎豆热研1号为材料,采用RT-PCR技术克隆了油莎豆脱水素基因CeDHN1包含完整ORF在内的708 bp cDNA序列。序列分析表明,CeDHN1预测编码235个氨基酸,理论分子量为25.97 kDa,等电点为5.2,总平均疏水指数为-1.383,不稳定系数为42.81,属于不稳定亲水蛋白。CeDHN1蛋白含有3个保守区域,即2个K片段和一个S片段,属于SKn型脱水素。亚细胞定位结果显示,CeDHN1定位在烟草叶片的细胞核。qRT-PCR分析显示,CeDHN1在油莎豆的根中的表达丰度最高,且显著高于其他组织。这些结果将为下一步的功能鉴定奠定坚实的基础。展开更多
基金funded by the Earmarked Fund for CARS(CARS-28-07)the Agricultural Variety Improvement Project of Shandong Province,China(2022LZGC011)the Qingdao Agricultural University Doctoral Start-Up Fund,China.
文摘Dehydrin(DHN)enhances plant resistance to environmental stress by regulating the synthesis of osmotic adjustment substances and scavenging reactive oxygen species.However,the role of PbDHN3 under salt stress remains unclear.In this study,salt stress induced high expression of PbDHN3,and the overexpression of PbDHN3(OE-PbDHN3)enhanced plant growth under salt stress compared to wild-type(WT)plants.OE-PbDHN3 plants exhibited higher chlorophyll content and root growth capacity than WT plants under salt stress.Transcriptome analysis revealed that PbDHN3 expression is associated with ethylene signaling pathways.OE-PbDHN3 transgenic plants substantially influenced ethylene content and the expression of related genes.Following treatment with exogenous ethephon,the transgenic lines notably inhibited the processes of ethylene synthesis and signaling transduction.OE-PbDHN3 transgenic lines treated with exogenous ethylene and the ethylene inhibitor 1-MCP demonstrated significant inhibition of ethylene synthesis and signaling transduction,while promoting root development and chlorophyll content.Under salt stress,OE-PbDHN3 downregulated the expression of ethylene biosynthesis genes PbACO1-like and PbACO2,and signal transduction genes PbEIN3-like during the initial stress phase.This early regulation mitigated the adverse effects of salt stress on the plants.These findings demonstrate that PbDHN3 ameliorates the ethylene-mediated plant growth phenotype under salt stress through regulation of ethylene synthesis and signal transduction.
基金supported by the National High Technology Research and Development Program of China(2001AA212161,2002AA224011)the National Natural Science Foundation of China(30170747).
文摘A full-length cDNA of dehydrin BcDh2 from Boea crassifolia and its antisense nucleotidesequence have been transferred into tobacco (Nicotiana tabacum) NC89 under the controlof a caulifower mosaic virus 35S promoter. Under a progressive water stress, photosyntheticrate, transpiration rate and stomatal conductance of the sense and antisense plantsreduced, and those of the control reduced much more. Photosynthetic rate, transpirationrate and stomatal conductance of all plants tested increased significantly 24 hourslater after recoveried water supply, and those of the sense and antisense plants werehigher than control. These indicated that overexpression of a dehydrin gene in tobaccomay improve tolerance to water stress for plants, however, antisense BcDh2 gene intransgenic plant did not influence physiological conditions. The results of germinationexperiment of the transgenic seeds showed that on MS medium with different concentrationPEG (8000), sense seed could more endure drought than control, while antisense seed wassensitive to drought. The results suggested that the overexpression of a dehydrin genein tobacco might improve the tolerance to water stress for plants.
文摘Drought is one of environmental stresses which the most limiting to plant growth and productivity. Drought stress led to a series of changes including biochemical changes like accumulation of osmolit and specific proteins involved in stress tolerance. One of the proteins that play a role in the mechanism of drought resistance is dehydrin protein. This study aimed to identify the protein profiles and dehydrin accumulation in 7 varieties of local Indonesian soybeans: Tanggamus, Nanti, Seulawah and Tidar (tolerant), Wilis and Burangrang (moderate) and Detam-1 (drought stress sensitive). Plants were treated with drought stress by adjusting soil water content to 25% below field capacity and compared with plants which were grown on normal condition as control plants. The results of SDS-PAGE electrophoresis showed a new protein with the molecular weight of 13 and 52 kDa were induced in Tanggamus, Nanti, Seulawah and Tidar varieties. Western blotting analysis for dehydrin showed that the quantity of the protein in the leaves of all varieties except Tanggamus decreased in drought stress conditions. The quantity of dehydrin protein in tolerant varieties higher than the protein quantity in both moderate varieties and drought sensitive.
基金This work was supported by the National Natural Science Foundation of China[Grant Nos.31870686,31971705,and 31670693]the Natural Science Foundation of Shanghai[Grant No.21ZR1434200].
文摘Dehydrins(DHNs),as members of the late embryogenesis abundant protein family,play critical roles in the protection of seeds from dehydration and plant adaptation to multiple abiotic stresses.Vitrification is a basic method in plant cryopreservation and is characterized by forming a glassy state to prevent lethal ice crystals produced during cryogenic storage.In this study,ApSK3 type DHN was genetically transformed into embryogenic calluses(EC)of Agapanthus praecox by overexpression(OE)and RNA interference(RNAi)techniques to evaluate the in vivo protective effect of DHNs during cryopreservation.The cell viability showed a completely opposite trend in OE and RNAi cell lines,the cell relative death ratio was decreased by 20.0%in ApSK3-OE EC and significantly increased by 66.15%in ApSK3-RNAi cells after cryopreservation.Overexpression of ApSK3 increased the content of non-enzymatic antioxidants(AsA and GSH)and up-regulated the expression of CAT,SOD,POD,and GPX genes,while ApSK3-RNAi cells decreased antioxidant enzyme activities and FeSOD,POD,and APX genes expression during cryopreservation.These findings suggest that ApSK3 affects ROS metabolism through chelating metal ions(Cu^(2+)and Fe^(3+)),alleviates H_(2)O_(2)and OH·excessive generation,activates the antioxidant system,and improves cellular REDOX balance and membrane lipid peroxidation damage of plant cells during cryopreservation.DHNs can effectively improve cell stress tolerance and have great potential for in vivo or in vitro applications in plant cryopreservation.
文摘A 500-bp cDNA fragment was amplified via RT-PCR from drought-induced total RNA of the drought-tolerant B. crassifolia Hemsl. using primers based on the sequence of published dehydrin conserved region. By using 5’ RACE, full-length coding region (1 148 bp) of BDN1 gene was produced. It is a new member of the dehydrin gene family. Southern analysis indicated that BDN1 is present in the 6. crassifolia genome as a single-copy gene. Northern analysis revealed that its expression is inducible by drought and cold stresses as well as ABA application.
文摘油莎豆播种期长、产量高,是目前已知唯一在块茎器官中积累大量油脂的特种经济作物,具有广阔的综合利用前景。为了进一步提高油莎豆的品质和产量,积极筛选和鉴定相关的抗性基因,研究基于转录组数据,以油莎豆热研1号为材料,采用RT-PCR技术克隆了油莎豆脱水素基因CeDHN1包含完整ORF在内的708 bp cDNA序列。序列分析表明,CeDHN1预测编码235个氨基酸,理论分子量为25.97 kDa,等电点为5.2,总平均疏水指数为-1.383,不稳定系数为42.81,属于不稳定亲水蛋白。CeDHN1蛋白含有3个保守区域,即2个K片段和一个S片段,属于SKn型脱水素。亚细胞定位结果显示,CeDHN1定位在烟草叶片的细胞核。qRT-PCR分析显示,CeDHN1在油莎豆的根中的表达丰度最高,且显著高于其他组织。这些结果将为下一步的功能鉴定奠定坚实的基础。
