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兜兰DEFICIENS(DEF)-和GLOBOSA(GLO)-like基因的克隆及表达分析 被引量:11
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作者 李冬梅 吕复兵 +3 位作者 朱根发 操君喜 李伟锋 孙映波 《西北植物学报》 CAS CSCD 北大核心 2012年第2期215-224,共10页
以‘同色兜兰’品种为材料,采用RT-PCR和RACE技术获得了DEFICIENS(DEF)-和GLOBOSA(GLO)-like基因的cDNA全长,命名为PcDEF和PcGLO,并用半定量RT-PCR和实时PCR研究了PcDEF和PcGLO在花芽发育过程和不同组织部位的表达特性。结果表明,PcDEF... 以‘同色兜兰’品种为材料,采用RT-PCR和RACE技术获得了DEFICIENS(DEF)-和GLOBOSA(GLO)-like基因的cDNA全长,命名为PcDEF和PcGLO,并用半定量RT-PCR和实时PCR研究了PcDEF和PcGLO在花芽发育过程和不同组织部位的表达特性。结果表明,PcDEF和PcGLO的全长cDNA分别为1 039bp和934bp,分别编码224和210个氨基酸;蛋白比对表明,PcDEF和PcGLO蛋白都具有典型MADS-box蛋白的MADS和K结构域;蛋白同源性分析显示,PcDEF和PcGLO与已登录的其它兰科植物的DEF/AP3和GLO/PI蛋白的相似性分别在75%~96%和87%~98%;系统进化树分析表明,PcDEF和PcGLO分别属于B类MADS-box蛋白家族的AP3和PI亚家族。表达分析显示,PcDEF和PcGLO在花芽发育中均有表达,PcDEF在成熟花、唇瓣和花瓣中的表达量高,在蕊柱、萼片、苞叶和根中次之,在花茎和叶中较低,在子房中几乎不表达;PcGLO在各组织中均有不同丰度的表达。 展开更多
关键词 兜兰 MADS-BOX基因 deficiens基因 GLOBOSA基因 RACE 进化分析 RT-PCR
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APETALA3/DEFICIENS和PISTILLATA/GLOBOSA基因与植物花发育 被引量:4
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作者 秦巧平 尹涛 +2 位作者 陈俊伟 谢鸣 张上隆 《细胞生物学杂志》 CSCD 2006年第4期571-576,共6页
APETALA3(AP3)/DEFICIENS(DEF)和PISTILLATA(PI)/GLOBOSA(GLO)为植物花器官发育B类基因,控制双子叶植物花瓣和雄蕊的发育,它们属于MADS-box基因家族,编码转录因子,这些基因的突变能导致花瓣转变为萼片,雄蕊转变为心皮。近年来已经在多... APETALA3(AP3)/DEFICIENS(DEF)和PISTILLATA(PI)/GLOBOSA(GLO)为植物花器官发育B类基因,控制双子叶植物花瓣和雄蕊的发育,它们属于MADS-box基因家族,编码转录因子,这些基因的突变能导致花瓣转变为萼片,雄蕊转变为心皮。近年来已经在多种植物中克隆到了AP3/DEF和PI/GLO基因,AP3/DEF和PI/GLO基因在拟南芥中只在花器官中表达,而在玉米等植物维管束、叶片等组织中也有表达。现对有关AP3/DEF和PI/GLO基因表达及其在植物系统发育学研究方面的进展进行综述。 展开更多
关键词 APETALA3/deficiens PISTILLATA/GLOBOSA 基因表达 花发育
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An ancient super allele of the Vrs1 gene driving the recent success in modern barley improvement through optimising spike architecture
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作者 Jingye Cheng Rui Pan +2 位作者 Wenying Zhang Tianhua He Chengdao Li 《Journal of Integrative Agriculture》 2026年第2期602-609,共8页
Improved yield potential is the goal of barley domestication and cultivation.During this process,two-and six-rowed barley types emerged and have been utilised in breeding and production.The six-rowed type could produc... Improved yield potential is the goal of barley domestication and cultivation.During this process,two-and six-rowed barley types emerged and have been utilised in breeding and production.The six-rowed type could produce three times as many grains as its ancestral two-rowed forms,thus dominating barley cultivation for thousands of years.The deficiens form of the two-rowed type,characterised by extremely suppressed lateral spikelets,has gained dominance over the past few decades in barley-growing regions worldwide.We hypothesised that the absence of lateral spikelets in deficiens barley affects spike architecture and spike-related traits,contributing to its superior yield potential of deficiens barley cultivation.Currently,a deficiens barley variety,RGT Planet,is the most popular barley variety in the world.In this study,we used two F_(2) populations derived from crossing RGT Planet with two canonical two-rowed barley and identified the functional allele Vrs1.t1 associated with deficiens morphology.We observed that the Vrs1.t1 allele may contribute to high yield potential by optimising spike architecture through increased spikelet length,grain number,and grain size.Phylogenetic analysis suggests that the deficiens mutation was likely present from the early stages of barley cultivation in the Fertile Crescent and spread to Ethiopia and beyond with agricultural expansion.We conclude that the ancient deficiens allele Vrs1.t1 has been a critical driver for the recent success of modern barley improvement by optimising spike architecture. 展开更多
关键词 deficiens barley ne mapping Vrs1 gene row types spike architecture yield potential
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Observation on the Application of Sijunzi Decoction in Spleen-stomach Qi Deficiency Syndrome of TCM Internal Diseases
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作者 LUJing 《外文科技期刊数据库(文摘版)医药卫生》 2022年第9期190-193,共4页
Objective: this paper mainly analyzes and studies the role and effect of Sijunzi Decoction in the treatment of spleen-stomach qi deficiency syndrome of TCM internal diseases. Methods: 100 patients with spleen-stomach ... Objective: this paper mainly analyzes and studies the role and effect of Sijunzi Decoction in the treatment of spleen-stomach qi deficiency syndrome of TCM internal diseases. Methods: 100 patients with spleen-stomach qi deficiency syndrome were selected in the hospital and randomly divided into two groups, one of which was treated by ordinary methods and the other by Sijunzi Decoction. After a period of time, check the physical conditions of the two groups of patients, and the contents are mainly divided into three aspects: The first is the clinical treatment effect of the patients, the second is the relapse of the patients, and the third is the adverse reactions of the patients. According to the comparison of the treatment results of two groups of patients, we can see the clinical application effect of Sijunzi Decoction. Results: comparing the results of the two groups of patients, it can be concluded that Sijunzi Decoction has great efficacy, which can not only improve the treatment effect of patients, but also reduce the probability of recurrence of diseases, and also has a strong inhibitory effect on adverse reactions of patients. Conclusion: through this study, it can be found that Sijunzi Decoction has a significant effect in the treatment of patients with spleen and stomach qi deficiency in internal medicine of traditional Chinese medicine, which can effectively improve the disease condition of patients. Therefore, it has a strong use value and is worthy of being widely used in the follow-up clinical treatment. 展开更多
关键词 sijunzi decoction traditional Chinese medicine internal medicine diseases spleen-stomach deficien
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