Functional proteomics is a powerful approach to globally investigate protein functions and their biological significance.Activity-based protein profiling(ABPP)has been widely applied to explore the activity and ligand...Functional proteomics is a powerful approach to globally investigate protein functions and their biological significance.Activity-based protein profiling(ABPP)has been widely applied to explore the activity and ligandability of proteins in complex biological systems.Herein,we summarized our efforts in developing chemical probes and chemical proteomic pipelines to identify protein targets of certain post-translational modifications(PTMs),covalent drugs,natural products and cellular metabolites.Furthermore,computer-aided proteomic strategies were developed to globally identify functional residues including hyper-reactive cysteines,active sites of selenoproteins and proximal anchors for protein activation.展开更多
基金The authors acknowledge the supports by the National Natu-ral Science Foundation of China(Nos.21925701,91953109 and 21778004).
文摘Functional proteomics is a powerful approach to globally investigate protein functions and their biological significance.Activity-based protein profiling(ABPP)has been widely applied to explore the activity and ligandability of proteins in complex biological systems.Herein,we summarized our efforts in developing chemical probes and chemical proteomic pipelines to identify protein targets of certain post-translational modifications(PTMs),covalent drugs,natural products and cellular metabolites.Furthermore,computer-aided proteomic strategies were developed to globally identify functional residues including hyper-reactive cysteines,active sites of selenoproteins and proximal anchors for protein activation.
