Objective To analyze the changes of intestinal flora of rats with minimal hepatic encephalopathy(MHE)after the treatment of Rhubarb Decoction using 16SrDNA sequencing technology,and to explore the changes of intestina...Objective To analyze the changes of intestinal flora of rats with minimal hepatic encephalopathy(MHE)after the treatment of Rhubarb Decoction using 16SrDNA sequencing technology,and to explore the changes of intestinal flora of MHE,as well as the target of action of Rhubarb Decoction.Methods Totally 45 rats were randomly divided into the normal control group(NC),the MHE model group(MHE),and the treatment group(MHEY)for the 30 g of Mume fructus and 30 g of vinegar-made Rhubarb,15 in each group.Rat in the MHE and HE groups were induced by intraperitoneal injection of tetrachloromethane(CCl,)and thioacetamide(TAA).After successful modeling,10 rats in each group were selected for comparative analysis.The water maze experiment was used to evaluate the learning and memory abilities of rats in each group before and after treatment,and biochemical indices were analyzed.16SrDNA sequencing technology was used to analyze the changes in the number,structure,and abundance of the intestinal flora of rats in each group.Results Compared with the NC group,the water maze test search time was prolonged,the levels of aspartate transaminase(AST),alanine aminotransferase(ALT),total bilirubin(TBIL),total bile acid(TBA),and blood ammonia increased,ALB decreased(P<0.05)in the MHEgroup.Compared with the MHE group,the water maze test search time was shortened,the levels of AST,ALT,TBIL,TBA,and blood ammonia decreased,ALB increased(P<0.05)in the MHEYgroup.16SrDNA technology revealed that,comparedwith group NC,Firmicutes andCyanobacteria were down-regulated,Bacteroidetes and Actinobacteria were up-regulated in MHE group and MHEY group.While the good bacteria proliferated in group MHEY,the detrimental bacteria rose dramaticallyin groupNMHE.AAdditionally,actinomyces in group MHEY had a declining tendency,and lactobacillus emerged as the dominating species following treatment of Rhubarb Decoction.The Clostridium genus,Clostridia order,Ruminococcaceae,Verrucomycetes family,Trichospiridae family,etc.were among the microbial markers of the MHEYYgroup.Conclusion Rhubarb Decoction can reduce the number of harmful intestinal flora in rats with mild hepatic encephalopathy.MHE can be recovered by acidifying the intestinal tract and restoring intestinal flora balance using the"Tongfu-opening method"of Chinese medicine.展开更多
文摘Objective To analyze the changes of intestinal flora of rats with minimal hepatic encephalopathy(MHE)after the treatment of Rhubarb Decoction using 16SrDNA sequencing technology,and to explore the changes of intestinal flora of MHE,as well as the target of action of Rhubarb Decoction.Methods Totally 45 rats were randomly divided into the normal control group(NC),the MHE model group(MHE),and the treatment group(MHEY)for the 30 g of Mume fructus and 30 g of vinegar-made Rhubarb,15 in each group.Rat in the MHE and HE groups were induced by intraperitoneal injection of tetrachloromethane(CCl,)and thioacetamide(TAA).After successful modeling,10 rats in each group were selected for comparative analysis.The water maze experiment was used to evaluate the learning and memory abilities of rats in each group before and after treatment,and biochemical indices were analyzed.16SrDNA sequencing technology was used to analyze the changes in the number,structure,and abundance of the intestinal flora of rats in each group.Results Compared with the NC group,the water maze test search time was prolonged,the levels of aspartate transaminase(AST),alanine aminotransferase(ALT),total bilirubin(TBIL),total bile acid(TBA),and blood ammonia increased,ALB decreased(P<0.05)in the MHEgroup.Compared with the MHE group,the water maze test search time was shortened,the levels of AST,ALT,TBIL,TBA,and blood ammonia decreased,ALB increased(P<0.05)in the MHEYgroup.16SrDNA technology revealed that,comparedwith group NC,Firmicutes andCyanobacteria were down-regulated,Bacteroidetes and Actinobacteria were up-regulated in MHE group and MHEY group.While the good bacteria proliferated in group MHEY,the detrimental bacteria rose dramaticallyin groupNMHE.AAdditionally,actinomyces in group MHEY had a declining tendency,and lactobacillus emerged as the dominating species following treatment of Rhubarb Decoction.The Clostridium genus,Clostridia order,Ruminococcaceae,Verrucomycetes family,Trichospiridae family,etc.were among the microbial markers of the MHEYYgroup.Conclusion Rhubarb Decoction can reduce the number of harmful intestinal flora in rats with mild hepatic encephalopathy.MHE can be recovered by acidifying the intestinal tract and restoring intestinal flora balance using the"Tongfu-opening method"of Chinese medicine.
