An octameric water moiety which consists of a chairlike water hexamer and two pendent water molecules in the 1,4-diaxial positions and shows a similar structure to the hydrocarbon （lr,4r）-1,4-dimethylcyclohexane, is...An octameric water moiety which consists of a chairlike water hexamer and two pendent water molecules in the 1,4-diaxial positions and shows a similar structure to the hydrocarbon （lr,4r）-1,4-dimethylcyclohexane, is unambiguously trapped in a 2D Cu（II） mixed-ligand coordination polymer, {[Cu2（bpp）2（HzO）2（bpda）2]＇6H2O}n （1） （bpp = 1,3-bis（4-pyridyl）propane and H2bpda = 2,2＇-biphenyldicarboxylic acid）. The water octamer can be extended into a hybrid carboxylate-water decamer when carboxylic oxygen atoms from bpda2- are involved. Interestingly, the present hybrid decamer bears a similar structural topology to a butterfly （H2O）10 cluster. The reversible dehydration/hydration of 1 is determined by X-ray powder diffraction studies.展开更多
Development of fingerprints based on DNA markers is necessary for proper identification and standardization of plant species. These techniques are widely used to develop an unquestionable method of plant identificatio...Development of fingerprints based on DNA markers is necessary for proper identification and standardization of plant species. These techniques are widely used to develop an unquestionable method of plant identification to protect the patents and quality control for industry. In this study, fifteen commercially important medicinal plants of Pakistan were collected from botanical garden of Qarshi Industries (Pvt.) Ltd, Pakistan. The objective was to optimize the extraction of genomic DNA for use in a PCR-based random amplified polymorphic DNA marker approach. The initial protocol used 60 decamers to amplify scorable amplicons;only nine markers produced significant bands in genomic DNA of medicinal plants. These markers generated 51 bands ranging between 250 and 1600 bp. The most important property of genomic markers is polymorphism to enable specific identification;all the used markers showed 100% polymorphism across 15 different plants. Further, six decamers amplified specific bands to reliably identify 8 species. The amplified bands were arranged in a binary matrix and analyzed by DNAMAN version 5.2.2 statistical software. A homology tree was constructed using binary data for nine markers, and four major clusters/clades were observed. The Rose, Mentha and Stevia accessions had shown clear clustering and grouped in major clusters/clads I, II and III respectively. Sixty decamers amplified 51 polymorphic loci in the genomes of 15 commercially valuable accessions. Moreover clear phylogenetic construction was observed in the generation of homolog tree. This protocol could therefore be useful to provide a baseline to authenticate, identify and perform phylogenetic analysis of important medicinal plants used in the Pakistani herbal medicine industry.展开更多
基金supported by the National Natural Science Foundation of China (50971063,21053001)the Natural Science Foundation of Fujian Province (2011J01047,2003F006,2010J01042)+1 种基金the Scientific Research Foundation for the Returned Overseas Chinese Scholars,State Education Ministry of Chinathe Startup Package Funding of Huaqiao University (10BS210 and 11BS102)
文摘An octameric water moiety which consists of a chairlike water hexamer and two pendent water molecules in the 1,4-diaxial positions and shows a similar structure to the hydrocarbon （lr,4r）-1,4-dimethylcyclohexane, is unambiguously trapped in a 2D Cu（II） mixed-ligand coordination polymer, {[Cu2（bpp）2（HzO）2（bpda）2]＇6H2O}n （1） （bpp = 1,3-bis（4-pyridyl）propane and H2bpda = 2,2＇-biphenyldicarboxylic acid）. The water octamer can be extended into a hybrid carboxylate-water decamer when carboxylic oxygen atoms from bpda2- are involved. Interestingly, the present hybrid decamer bears a similar structural topology to a butterfly （H2O）10 cluster. The reversible dehydration/hydration of 1 is determined by X-ray powder diffraction studies.
文摘Development of fingerprints based on DNA markers is necessary for proper identification and standardization of plant species. These techniques are widely used to develop an unquestionable method of plant identification to protect the patents and quality control for industry. In this study, fifteen commercially important medicinal plants of Pakistan were collected from botanical garden of Qarshi Industries (Pvt.) Ltd, Pakistan. The objective was to optimize the extraction of genomic DNA for use in a PCR-based random amplified polymorphic DNA marker approach. The initial protocol used 60 decamers to amplify scorable amplicons;only nine markers produced significant bands in genomic DNA of medicinal plants. These markers generated 51 bands ranging between 250 and 1600 bp. The most important property of genomic markers is polymorphism to enable specific identification;all the used markers showed 100% polymorphism across 15 different plants. Further, six decamers amplified specific bands to reliably identify 8 species. The amplified bands were arranged in a binary matrix and analyzed by DNAMAN version 5.2.2 statistical software. A homology tree was constructed using binary data for nine markers, and four major clusters/clades were observed. The Rose, Mentha and Stevia accessions had shown clear clustering and grouped in major clusters/clads I, II and III respectively. Sixty decamers amplified 51 polymorphic loci in the genomes of 15 commercially valuable accessions. Moreover clear phylogenetic construction was observed in the generation of homolog tree. This protocol could therefore be useful to provide a baseline to authenticate, identify and perform phylogenetic analysis of important medicinal plants used in the Pakistani herbal medicine industry.
