实际电力系统运行工况复杂多变,存在各种不确定性因素,现有继电保护模型在构建时,往往难以全面准确地考虑这些复杂因素,导致模型与实际系统运行情况存在偏差。文章聚焦基于分布式数字实时仿真(Distributed Digital Real-Time Simulation...实际电力系统运行工况复杂多变,存在各种不确定性因素,现有继电保护模型在构建时,往往难以全面准确地考虑这些复杂因素,导致模型与实际系统运行情况存在偏差。文章聚焦基于分布式数字实时仿真(Distributed Digital Real-Time Simulation,DDRTS)图模库一体化技术的继电保护模型构建研究。运用DDRTS图模库一体化技术,通过搭建一次系统图形模型、二次系统及保护数字模型,并建立模型关联配置数据库,构建完整的继电保护模型。经实验验证,该模型性能良好,能满足电力系统对继电保护的严苛要求,为电力系统安全稳定运行提供有力支撑,也为相关领域技术研究提供新思路。展开更多
The differentiation of human leukemia K562 cells can be induced by matrine. To investigate the mechanism of the induced cell differentiation, an improved DDRT PCR technique was applied to screen the differentially exp...The differentiation of human leukemia K562 cells can be induced by matrine. To investigate the mechanism of the induced cell differentiation, an improved DDRT PCR technique was applied to screen the differentially expressed genes between K562 cells and the matrine treated cells. The sequences of the fragments of the differentially expressed genes were obtained by sequencing following the T A cloned fragments identified by restriction endonuclease, and analyzed with bioinformatics. The results indicated that the multiple gene expression known already changed in the process of induced cell differentiation and an unknown gene fragment was cloned. It suggested that the differentially expressed genes acted as cell differentiation related genes involved in the early phase of the induced cell differentiation.展开更多
[Objective] The aim was to explore the reasons of false positives in Different Display Reverse Transcription（DDRT）analysis.[Method] Soybean varieties ＂Jilin 30＂ and ＂Tongnong 13＂ were used as materials to carry ...[Objective] The aim was to explore the reasons of false positives in Different Display Reverse Transcription（DDRT）analysis.[Method] Soybean varieties ＂Jilin 30＂ and ＂Tongnong 13＂ were used as materials to carry out analysis on false positives in DDRT analysis.[Result] An important origin of false positives appeared in DDRT analysis was the non-specific amplification caused by the combination of single primer and cDNA.The parallel PCR test of single primer should be set so as to verify whether the obtained fragments were the false positives or the PCR productions combined with single primer.[Conclusion] This study had provided basis for improving the success rate of DDRT experiment.展开更多
文摘实际电力系统运行工况复杂多变,存在各种不确定性因素,现有继电保护模型在构建时,往往难以全面准确地考虑这些复杂因素,导致模型与实际系统运行情况存在偏差。文章聚焦基于分布式数字实时仿真(Distributed Digital Real-Time Simulation,DDRTS)图模库一体化技术的继电保护模型构建研究。运用DDRTS图模库一体化技术,通过搭建一次系统图形模型、二次系统及保护数字模型,并建立模型关联配置数据库,构建完整的继电保护模型。经实验验证,该模型性能良好,能满足电力系统对继电保护的严苛要求,为电力系统安全稳定运行提供有力支撑,也为相关领域技术研究提供新思路。
文摘The differentiation of human leukemia K562 cells can be induced by matrine. To investigate the mechanism of the induced cell differentiation, an improved DDRT PCR technique was applied to screen the differentially expressed genes between K562 cells and the matrine treated cells. The sequences of the fragments of the differentially expressed genes were obtained by sequencing following the T A cloned fragments identified by restriction endonuclease, and analyzed with bioinformatics. The results indicated that the multiple gene expression known already changed in the process of induced cell differentiation and an unknown gene fragment was cloned. It suggested that the differentially expressed genes acted as cell differentiation related genes involved in the early phase of the induced cell differentiation.
文摘[Objective] The aim was to explore the reasons of false positives in Different Display Reverse Transcription（DDRT）analysis.[Method] Soybean varieties ＂Jilin 30＂ and ＂Tongnong 13＂ were used as materials to carry out analysis on false positives in DDRT analysis.[Result] An important origin of false positives appeared in DDRT analysis was the non-specific amplification caused by the combination of single primer and cDNA.The parallel PCR test of single primer should be set so as to verify whether the obtained fragments were the false positives or the PCR productions combined with single primer.[Conclusion] This study had provided basis for improving the success rate of DDRT experiment.
