Objective:Human diphyllobothriosis is a global fish-borne zoonosis affecting approximately 20 million people.This study reports the first human case of Dibothriocephalus dendriticus(D.dendriticus)in China and explores...Objective:Human diphyllobothriosis is a global fish-borne zoonosis affecting approximately 20 million people.This study reports the first human case of Dibothriocephalus dendriticus(D.dendriticus)in China and explores its epidemiological and phylogenetic implications.Methods:Morphological features of eggs and proglottids were examined.The mitochondrial cox1 gene was sequenced for species identification.Phylogenetic analysis and epidemiological data were analyzed to trace the infection source.Results:The expelled tapeworm measured 50 cm in length and 0.7 cm in width.The gravid proglottid was longer than wide,with a centrally positioned uterus.Eggs measured 63.29±1.17×48.31±0.94μm(n=15)and had an operculum.The cox1 gene(PQ169609)showed 99.87%homology with D.dendriticus(AM412738.2).Morphological and molecular analyses confirmed the parasite as D.dendriticus.Consumption of raw salmon in Hong Kong Special Administrative Region(May 2023)and raw trout in Beijing Municipality(August 2022)were identified as potential infection sources.Phylogenetic analysis linked the strain to one from UK fish(KY552870),suggesting a common origin.Conclusion:This study reports the first human case of D.dendriticus in China.It highlights the emerging threat of D.dendriticus amid globalization and rising fish consumption.Strengthening food safety measures is essential to reducing infection risk.展开更多
基金Supported by the Three-Year Initiative Plan for Strengthening Public Health System Construction in Shanghai(2023-2025)Key Discipline Project(GWVI-11.1-12)the National Parasite Resource Center(NPRC-2019-194-30).
文摘Objective:Human diphyllobothriosis is a global fish-borne zoonosis affecting approximately 20 million people.This study reports the first human case of Dibothriocephalus dendriticus(D.dendriticus)in China and explores its epidemiological and phylogenetic implications.Methods:Morphological features of eggs and proglottids were examined.The mitochondrial cox1 gene was sequenced for species identification.Phylogenetic analysis and epidemiological data were analyzed to trace the infection source.Results:The expelled tapeworm measured 50 cm in length and 0.7 cm in width.The gravid proglottid was longer than wide,with a centrally positioned uterus.Eggs measured 63.29±1.17×48.31±0.94μm(n=15)and had an operculum.The cox1 gene(PQ169609)showed 99.87%homology with D.dendriticus(AM412738.2).Morphological and molecular analyses confirmed the parasite as D.dendriticus.Consumption of raw salmon in Hong Kong Special Administrative Region(May 2023)and raw trout in Beijing Municipality(August 2022)were identified as potential infection sources.Phylogenetic analysis linked the strain to one from UK fish(KY552870),suggesting a common origin.Conclusion:This study reports the first human case of D.dendriticus in China.It highlights the emerging threat of D.dendriticus amid globalization and rising fish consumption.Strengthening food safety measures is essential to reducing infection risk.
