Cymbaria daurica L. is a well-known traditional Mongolian medicine, which has been used to treat diabetesrelated conditions characterized by persistent thirst and hunger, copious urination, and weight loss. We aimed t...Cymbaria daurica L. is a well-known traditional Mongolian medicine, which has been used to treat diabetesrelated conditions characterized by persistent thirst and hunger, copious urination, and weight loss. We aimed to investigate the protective effects of C. daurica extracts and phenylethanoid glycosides including verbascoside and isoacteoside on INS-1 cells. We discovered phenylethanoid glycosides from n-butanol extract with large content through extraction and separation. We continue to study the protective effects of phenylethanoid glycosides including verbascoside and isoacteoside on INS-1 cells. INS-1 cells were treated with C. daurica, cell viability assay, RNA-seq technology, superoxide dismutase activity and malonaldehyde content, quantitative real time-PCR and Western blot analysis were used to study the protective effects of C. daurica. Cell viability assay resulted that n-butanol extract and verbascoside, isoacteoside showed protective effects of C. daurica. According to the RNA-seq technology to identify the differentially expressed genes in INS-1 cells, the pathway of gene enrich the protective effect of C. daurica on oxidative stress. SOD activity and the content of MDA indicated that C. daurica could enhance the antioxidant capacity of INS-1 cells. Further investigation indicated C. daurica alleviate oxidative stress by inhibiting INS-1 cell apoptosis. C. daurica may play an anti-diabetic role by inhibiting islet cell apoptosis.展开更多
Cymbaria daurica L.has a long history as a folk medicine and tea for the treatment of diabetes.However,the biological activity and mechanism of its hypoglycemic effect have not been fully elucidated.In this study,the ...Cymbaria daurica L.has a long history as a folk medicine and tea for the treatment of diabetes.However,the biological activity and mechanism of its hypoglycemic effect have not been fully elucidated.In this study,the potential mechanism of C.daurica against type 2 diabetes mellitus(T2DM)was postulated via pharmacological network analysis.Based on data mining techniques involving topological parameters,gene ontology,and pathway enrichment,we established a compound-target,protein-protein interaction,and target-pathway network to identify central targets and pathways.Pathway enrichment analysis revealed that the most important pathway associated with C.daurica in treating T2DM is the PI3K-Akt signaling pathway.Molecular docking was performed to validate the predicted results.Then,a HepG2 cell insulin resistance model and a high-fat,high-glucose diet combined with a streptozotocin-induced T2DM rat model was established to assess the fasting glucose changes and lipid profile after C.daurica treatment,respectively.Finally,real-time PCR and western blotting were used to verify changes in key targets.The anti-diabetic mechanism of the active ingredient in C.daurica may involve the regulation of IRS-2,Akt1,GLUT4,and GSK3β.展开更多
The forkhead box O(FoxO),as a conserved transcription factor,plays an indispensable role in regulating insect diapause.However,how FoxO is regulated to control diapause in insects remains unknown.In this study,we disc...The forkhead box O(FoxO),as a conserved transcription factor,plays an indispensable role in regulating insect diapause.However,how FoxO is regulated to control diapause in insects remains unknown.In this study,we discovered functional binding sites for miR-2765-3p in the 3′untranslated region of FoxO in Galeruca daurica.The luciferase reporter assay showed that miR-2765-3p targeted FoxO and suppressed its expression.The expression profiles of miR-2765-3p and FoxO displayed opposite patterns during the female developmental process.Overexpression of miR-2765-3p by the injection of the miR-2765-3p agomir into adult females reduced FoxO expression,leading to the suppression of lipid accumulation,promotion of ovarian development,and inhibition of reproductive diapause.This is similar to the phenotype that results from the depletion of FoxO by injecting dsFoxO into adult females.In addition,the repression of miR-2765-3p by injecting the miR-2765-3p antagomir increased the FoxO transcript level,leading to the stimulation of lipid accumulation,depression of ovarian development,and induction of reproductive diapause.A hormone injection assay showed that the juvenile hormone(JH)agonist(methoprene)upregulated miR-2765-3p and downregulated FoxO.Notably,injecting methoprene rescued ovarian development defects associated with miR-2765-3p inhibition.These findings indicate that the JH/miR-2765-3p/FoxO axis plays a vital role in the regulation of reproductive diapause in G.daurica.展开更多
Durante il mese di agosto 1993 si è osservata ripetutamente una coppia adulta,con quattro giovani già volanti,di Rondine rossiccia Hirundo daurica nel comunedi Gargnano (tav.IGMI 35 Ⅲ SE,Gargnano).Le osserv...Durante il mese di agosto 1993 si è osservata ripetutamente una coppia adulta,con quattro giovani già volanti,di Rondine rossiccia Hirundo daurica nel comunedi Gargnano (tav.IGMI 35 Ⅲ SE,Gargnano).Le osservazioni si sono protratte dal 18 al 27 agosto,in un oliveto coltivato,esposto a SE e situato tra la via Prea e la strada statale 45 bis《Gardesana occidenta-le》,a circa 150 m s.l.m.I giovani erano già in grado di cacciare insetti in volo,ma ritornavano spesso al posatoio abituale costituito da un tratto di cavo telefonicoattraversante l’oliveto,dove venivano ancora imbeccati dai genitori.Durante il展开更多
基金funded by the National Natural Science Foundation of China (81760776)the Natural Science Foundation of Inner Mongolia Autonomous Region (2018ZD13)+2 种基金the Natural Science Foundation of Inner Mongolia Autonomous Region (2020MS08070)Science and technology development projects in key areas of Baotou science and technology plan (2020Z1016-3)Natural Science Foundation Project of Inner Mongolia Autonomous Region (2021MS03025)。
文摘Cymbaria daurica L. is a well-known traditional Mongolian medicine, which has been used to treat diabetesrelated conditions characterized by persistent thirst and hunger, copious urination, and weight loss. We aimed to investigate the protective effects of C. daurica extracts and phenylethanoid glycosides including verbascoside and isoacteoside on INS-1 cells. We discovered phenylethanoid glycosides from n-butanol extract with large content through extraction and separation. We continue to study the protective effects of phenylethanoid glycosides including verbascoside and isoacteoside on INS-1 cells. INS-1 cells were treated with C. daurica, cell viability assay, RNA-seq technology, superoxide dismutase activity and malonaldehyde content, quantitative real time-PCR and Western blot analysis were used to study the protective effects of C. daurica. Cell viability assay resulted that n-butanol extract and verbascoside, isoacteoside showed protective effects of C. daurica. According to the RNA-seq technology to identify the differentially expressed genes in INS-1 cells, the pathway of gene enrich the protective effect of C. daurica on oxidative stress. SOD activity and the content of MDA indicated that C. daurica could enhance the antioxidant capacity of INS-1 cells. Further investigation indicated C. daurica alleviate oxidative stress by inhibiting INS-1 cell apoptosis. C. daurica may play an anti-diabetic role by inhibiting islet cell apoptosis.
基金funded by the National Natural Science Foundation of China(81760776)the Natural Science Foundation of Inner Mongolia Autonomous Region(2018ZD13,2018LH08082,2019MS08208).
文摘Cymbaria daurica L.has a long history as a folk medicine and tea for the treatment of diabetes.However,the biological activity and mechanism of its hypoglycemic effect have not been fully elucidated.In this study,the potential mechanism of C.daurica against type 2 diabetes mellitus(T2DM)was postulated via pharmacological network analysis.Based on data mining techniques involving topological parameters,gene ontology,and pathway enrichment,we established a compound-target,protein-protein interaction,and target-pathway network to identify central targets and pathways.Pathway enrichment analysis revealed that the most important pathway associated with C.daurica in treating T2DM is the PI3K-Akt signaling pathway.Molecular docking was performed to validate the predicted results.Then,a HepG2 cell insulin resistance model and a high-fat,high-glucose diet combined with a streptozotocin-induced T2DM rat model was established to assess the fasting glucose changes and lipid profile after C.daurica treatment,respectively.Finally,real-time PCR and western blotting were used to verify changes in key targets.The anti-diabetic mechanism of the active ingredient in C.daurica may involve the regulation of IRS-2,Akt1,GLUT4,and GSK3β.
基金supported by the National Natural Science Foundation of China(31760517).
文摘The forkhead box O(FoxO),as a conserved transcription factor,plays an indispensable role in regulating insect diapause.However,how FoxO is regulated to control diapause in insects remains unknown.In this study,we discovered functional binding sites for miR-2765-3p in the 3′untranslated region of FoxO in Galeruca daurica.The luciferase reporter assay showed that miR-2765-3p targeted FoxO and suppressed its expression.The expression profiles of miR-2765-3p and FoxO displayed opposite patterns during the female developmental process.Overexpression of miR-2765-3p by the injection of the miR-2765-3p agomir into adult females reduced FoxO expression,leading to the suppression of lipid accumulation,promotion of ovarian development,and inhibition of reproductive diapause.This is similar to the phenotype that results from the depletion of FoxO by injecting dsFoxO into adult females.In addition,the repression of miR-2765-3p by injecting the miR-2765-3p antagomir increased the FoxO transcript level,leading to the stimulation of lipid accumulation,depression of ovarian development,and induction of reproductive diapause.A hormone injection assay showed that the juvenile hormone(JH)agonist(methoprene)upregulated miR-2765-3p and downregulated FoxO.Notably,injecting methoprene rescued ovarian development defects associated with miR-2765-3p inhibition.These findings indicate that the JH/miR-2765-3p/FoxO axis plays a vital role in the regulation of reproductive diapause in G.daurica.
文摘Durante il mese di agosto 1993 si è osservata ripetutamente una coppia adulta,con quattro giovani già volanti,di Rondine rossiccia Hirundo daurica nel comunedi Gargnano (tav.IGMI 35 Ⅲ SE,Gargnano).Le osservazioni si sono protratte dal 18 al 27 agosto,in un oliveto coltivato,esposto a SE e situato tra la via Prea e la strada statale 45 bis《Gardesana occidenta-le》,a circa 150 m s.l.m.I giovani erano già in grado di cacciare insetti in volo,ma ritornavano spesso al posatoio abituale costituito da un tratto di cavo telefonicoattraversante l’oliveto,dove venivano ancora imbeccati dai genitori.Durante il
